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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHA609
Library SH
(Link to library)
Clone ID SHA609
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11113-1|Contig-U11615-1
Original site URL
Representative seq. ID SHA609P
(Link to Original site)
Representative DNA sequence 
>SHA609 (SHA609Q) /CSM/SH/SHA6-A/SHA609Q.Seq.d/
AATATAAATTTATATCTATATCACACATTGTTTTTATTTCATAATTTCATACACCCACTT
TTTTTTTTTTATGACNCAAAANCANTTTAAATAAAAATAAATTTNCCGNTNTTTGTAAAA
ATAANTAAAAAAAAAACCAANTAAAAAACCCCNTAAAAAAAAAAXXXXXXXXXXGCAAAA
TCAACCCGTTGATAAATGTGAAATTGCCATTAAAAAGATTGCACATGTATCAGCAAAAGC
ACAAAGAACCAATCTCAATGAGATTGGATTTTTAAATTTCTGTAAGCATCCAAACATTGT
CAGTTACTTAAGAAGTCATTTGGTTGATGATACCATTTGGATCGCTATGGAGTATATGCA
AGGTGGCACTTTAACCGAAGCAAGTCAAGGCCACACTTTCAATGAGTCGTGTATCGCTTA
CGTTGCAAAGGGTATGTTGGAAGGCTTAATGTATTTACATGCTCACAACATCGTTCATAG
GGATATTAAATCGGGTAACATTATGATGACCATCGATGGTAAAATCAAGATTGTCGATTT
CGGTCTTTGTGTCGACGCAAATGAAAGAAAATTAGTACATATGGCAGGTTCCCCTTTTTG
GATGTCTCCAGAGATGATTCGTGGTGAGTCCTATGGCTGTCCAACCGATATTTGGAGTTT
TGCAATTTGTCTTTTGGAATTGGCAAATGGTGAACCACCCCACAGAAAATCTTCCCTCCC
CTNCTATGTTCACCNCTGCAACTGAGGGNTGTGCAGGTNTGGACAAACCTGAAAGATGGA
CCGAACACTTTACTCATTTCTTAAATCTTTGTTTAGAAATGGATCCTTCAAAGAGATCAA
CTGCCGAACAACTTTTAAAACATCCTTGNATTAATTTATCTGAAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
i*iyiyithcfyfiisythffffmtqxxfk*k*ixrxl*k*XKKKPXKKPXKKK---

---QNQPVDKCEIAIKKIAHVSAKAQRTNLNEIGFLNFCKHPNIVSYLRSHLVDDTIWIA
MEYMQGGTLTEASQGHTFNESCIAYVAKGMLEGLMYLHAHNIVHRDIKSGNIMMTIDGKI
KIVDFGLCVDANERKLVHMAGSPFWMSPEMIRGESYGCPTDIWSFAICLLELANGEPPHR
KSSLPXYVHXCN*gxcrxgqt*kmdrtlysflkslfrngsfkeincrttfktslx*fi*k
Translated Amino Acid sequence (All Frames) 
Frame A:
ninlylyhtlflfhnfihplfffydxkxx*ikinxpxfvkixkkktx*ktp*kk---

---akstr**m*nch*kdctciskstknqsq*dwifkfl*askhcqllkksfg**yhldr
ygvyarwhfnrsksrphfq*vvyrlrckgyvgrlnvftcsqhrs*gy*ig*hyddhrw*n
qdcrfrslcrrk*kkistygrfpfldvsrddsw*vlwlsnrylefcnlsfgigkw*ttpq
kifppllcsplqlrxvqvwtnlkdgpntllis*ifv*kwilqrdqlpnnf*nilxliylk

Frame B:
i*iyiyithcfyfiisythffffmtqxxfk*k*ixrxl*k*XKKKPXKKPXKKK---

---QNQPVDKCEIAIKKIAHVSAKAQRTNLNEIGFLNFCKHPNIVSYLRSHLVDDTIWIA
MEYMQGGTLTEASQGHTFNESCIAYVAKGMLEGLMYLHAHNIVHRDIKSGNIMMTIDGKI
KIVDFGLCVDANERKLVHMAGSPFWMSPEMIRGESYGCPTDIWSFAICLLELANGEPPHR
KSSLPXYVHXCN*gxcrxgqt*kmdrtlysflkslfrngsfkeincrttfktslx*fi*k

Frame C:
ykfisishivfis*fhtptfffl*xkxxlnknkfxxxcknx*kknqxknpxkkk---

---kinplinvklplkrlhmyqqkhkepismrldf*isvsiqtlsvt*eviwlmipfgsl
wsickval*pkqvkatlsmsrvsltlqrvcwka*ciymlttsfigilnrvtl**psmvks
rlsisvfvstqmken*yiwqvplfgclqr*fvvspmavqpifgvlqfvfwnwqmvnhpte
nlpspxmftxategcagxdkperwtehfthflnlclemdpskrstaeqllkhpxinlsen
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHA609 (SHA609Q) /CSM/SH/SHA6-A/SHA609Q.Seq.d/                       1384   0.0
AHH860 (AHH860Q) /CSM/AH/AHH8-C/AHH860Q.Seq.d/                       1344   0.0
SHA357 (SHA357Q) /CSM/SH/SHA3-C/SHA357Q.Seq.d/                       1342   0.0
SHC466 (SHC466Q) /CSM/SH/SHC4-C/SHC466Q.Seq.d/                       1336   0.0
AHM540 (AHM540Q) /CSM/AH/AHM5-B/AHM540Q.Seq.d/                       1332   0.0
SHA833 (SHA833Q) /CSM/SH/SHA8-B/SHA833Q.Seq.d/                       1330   0.0
AHM337 (AHM337Q) /CSM/AH/AHM3-B/AHM337Q.Seq.d/                       1328   0.0
AHK894 (AHK894Q) /CSM/AH/AHK8-D/AHK894Q.Seq.d/                       1328   0.0
CHI388 (CHI388Q) /CSM/CH/CHI3-D/CHI388Q.Seq.d/                       1326   0.0
AHO687 (AHO687Q) /CSM/AH/AHO6-D/AHO687Q.Seq.d/                       1326   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF238312|AF238312.1 Dictyostelium discoideum putative serine-threonine protein kinase MkcB (mkcB) gene, complete cds.    62   2e-18  5
U60169|U60169.1 Dictyostelium discoideum putative protein kinase MkcA (mkcA) gene, complete cds.    36   0.020  4
CO370227|CO370227.1 RTK1_66_H12.b1_A029 Roots minus potassium Pinus taeda cDNA clone RTK1_66_H12_A029 3', mRNA sequence.    48   0.32   1
AF093689|AF093689.1 Dictyostelium discoideum MEK kinase alpha (mkkA) mRNA, complete cds.    34   0.81   2
CK255071|CK255071.1 EST738708 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCCI38 5' end, mRNA sequence.    46   1.3    1
CK254711|CK254711.1 EST738348 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCCF56 3' end, mRNA sequence.    46   1.3    1
CK254710|CK254710.1 EST738347 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCCF56 5' end, mRNA sequence.    46   1.3    1
CK254079|CK254079.1 EST737716 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCCA43 5' end, mRNA sequence.    46   1.3    1
CK254025|CK254025.1 EST737662 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCCA01 5' end, mRNA sequence.    46   1.3    1
CK252149|CK252149.1 EST735786 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCBS95 3' end, mRNA sequence.    46   1.3    1
dna update 2005.12.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF092132_1(AF092132|pid:none) Homo sapiens PAK2 mRNA, complete c...   151   6e-44
AM943852_1(AM943852|pid:none) Homo sapiens mRNA for PAK3cb prote...   147   8e-43
AM943853_1(AM943853|pid:none) Mus musculus mRNA for PAK3c protei...   147   8e-43
(Q7YQL3) RecName: Full=Serine/threonine-protein kinase PAK 3;   ...   147   8e-43
(Q61036) RecName: Full=Serine/threonine-protein kinase PAK 3;   ...   147   8e-43
AF155651_1(AF155651|pid:none) Human P21 activated kinase-3 mRNA.      147   8e-43
AF082297_1(AF082297|pid:none) Mus musculus Cdc42/Rac effector ki...   147   8e-43
AJ496262_1(AJ496262|pid:none) Mus musculus mRNA for p21-activate...   147   8e-43
AF485330_1(AF485330|pid:none) Xenopus laevis Pak3 serine/threoni...   147   1e-42
AM943855_1(AM943855|pid:none) Xenopus laevis mRNA for PAK3a prot...   147   1e-42
protein update 2009. 4.14
PSORT 

 psg: 0.37  gvh: 0.30  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 24.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for SHA609 is nuc
5' end seq. ID SHA609F
5' end seq. 
>SHA609F.Seq
AATATAAATTTATATCTATATCACACATTGTTTTTATTTCATAATTTCATACACCCACTT
TTTTTTTTTTATGACNCAAAANCANTTTAAATAAAAATAAATTTNCCGNTNTTTGTAAAA
ATAANTAAAAAAAAAACCAANTAAAAAACCCCNTAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 174
3' end seq. ID SHA609Z
3' end seq. 
>SHA609Z.Seq
NNNNNNNNNNGCAAAATCAACCCGTTGATAAATGTGAAATTGCCATTAAAAAGATTGCAC
ATGTATCAGCAAAAGCACAAAGAACCAATCTCAATGAGATTGGATTTTTAAATTTCTGTA
AGCATCCAAACATTGTCAGTTACTTAAGAAGTCATTTGGTTGATGATACCATTTGGATCG
CTATGGAGTATATGCAAGGTGGCACTTTAACCGAAGCAAGTCAAGGCCACACTTTCAATG
AGTCGTGTATCGCTTACGTTGCAAAGGGTATGTTGGAAGGCTTAATGTATTTACATGCTC
ACAACATCGTTCATAGGGATATTAAATCGGGTAACATTATGATGACCATCGATGGTAAAA
TCAAGATTGTCGATTTCGGTCTTTGTGTCGACGCAAATGAAAGAAAATTAGTACATATGG
CAGGTTCCCCTTTTTGGATGTCTCCAGAGATGATTCGTGGTGAGTCCTATGGCTGTCCAA
CCGATATTTGGAGTTTTGCAATTTGTCTTTTGGAATTGGCAAATGGTGAACCACCCCACA
GAAAATCTTCCCTCCCCTNCTATGTTCACCNCTGCAACTGAGGGNTGTGCAGGTNTGGAC
AAACCTGAAAGATGGACCGAACACTTTACTCATTTCTTAAATCTTTGTTTAGAAATGGAT
CCTTCAAAGAGATCAACTGCCGAACAACTTTTAAAACATCCTTGNATTAATTTATCTGAA
AAT
Length of 3' end seq. 723
Connected seq. ID SHA609P
Connected seq. 
>SHA609P.Seq
AATATAAATTTATATCTATATCACACATTGTTTTTATTTCATAATTTCATACACCCACTT
TTTTTTTTTTATGACNCAAAANCANTTTAAATAAAAATAAATTTNCCGNTNTTTGTAAAA
ATAANTAAAAAAAAAACCAANTAAAAAACCCCNTAAAAAAAAAA----------GCAAAA
TCAACCCGTTGATAAATGTGAAATTGCCATTAAAAAGATTGCACATGTATCAGCAAAAGC
ACAAAGAACCAATCTCAATGAGATTGGATTTTTAAATTTCTGTAAGCATCCAAACATTGT
CAGTTACTTAAGAAGTCATTTGGTTGATGATACCATTTGGATCGCTATGGAGTATATGCA
AGGTGGCACTTTAACCGAAGCAAGTCAAGGCCACACTTTCAATGAGTCGTGTATCGCTTA
CGTTGCAAAGGGTATGTTGGAAGGCTTAATGTATTTACATGCTCACAACATCGTTCATAG
GGATATTAAATCGGGTAACATTATGATGACCATCGATGGTAAAATCAAGATTGTCGATTT
CGGTCTTTGTGTCGACGCAAATGAAAGAAAATTAGTACATATGGCAGGTTCCCCTTTTTG
GATGTCTCCAGAGATGATTCGTGGTGAGTCCTATGGCTGTCCAACCGATATTTGGAGTTT
TGCAATTTGTCTTTTGGAATTGGCAAATGGTGAACCACCCCACAGAAAATCTTCCCTCCC
CTNCTATGTTCACCNCTGCAACTGAGGGNTGTGCAGGTNTGGACAAACCTGAAAGATGGA
CCGAACACTTTACTCATTTCTTAAATCTTTGTTTAGAAATGGATCCTTCAAAGAGATCAA
CTGCCGAACAACTTTTAAAACATCCTTGNATTAATTTATCTGAAAAT
Length of connected seq. 877
Full length Seq ID -
Full length Seq. -
Length of full length seq. -