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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI740
Library AF
(Link to library)
Clone ID AFI740
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13845-1
Original site URL
Representative seq. ID AFI740P
(Link to Original site)
Representative DNA sequence 
>AFI740 (AFI740Q) /CSM/AF/AFI7-B/AFI740Q.Seq.d/
ATNACACACACAAAAACATTTTTAGATAATTATTTAGACCACACACATTTTACACGCACG
CACAATACGTTAGAATATATAATTATATTAATATACGATAAAAAAACATTATACAGCAAA
CGCAAATTATTGTATCTTTCAAATTAAAAAAAAAAXXXXXXXXXXATATTCCAGATTTAG
ATGATTTCCAAGATGATAATATAATTGAAGAAGAGGATCCAGCTGTATTATCAAAAAATA
ATAAAACAACAACTACTACAACAGCTAACAACAATAATAATAATAATTCAGAAAATAAAG
TTGAAGATAATGATAATATTTTACGNACTCGNACATATGATATTTCAATCACTTATGATA
AATATTATCAAACNCCAAGAGTTTGGTTATTTGGATATGATGAAAATAGAAAACCATTGA
AACCAGAAGAAATCTTTGAAGATATTTCAGAAGATCATGCACATAAAACTGTCACAATCG
ATAGCCACCCACATTTAGGTATNTCATTTGCATACATTCATCCATGTAGACATGCTGCCG
TCATGAAAAAATTGGTTGATAGACAATCCGAAAATGGTAAAGAAC
sequence update 2001. 6. 2
Translated Amino Acid sequence 
xhtqkhf*iii*tthilhartir*ni*ly*YTIKKHYTANANYCIFQIKKK---

---IPDLDDFQDDNIIEEEDPAVLSKNNKTTTTTTANNNNNNNSENKVEDNDNILRTRTY
DISITYDKYYQTPRVWLFGYDENRKPLKPEEIFEDISEDHAHKTVTIDSHPHLGXSFAYI
HPCRHAAVMKKLVDRQSENGKE
Translated Amino Acid sequence (All Frames) 
Frame A:
xthtktfldnyldhthftrthntleyiiiliydkktlyskrkllylsn*kk---

---ifqi*miskmii*lkkriqlyyqkiikqqllqqlttiiiiiqkiklkimiifyxlxh
mifqslminiikxqefgyldmmkienh*nqkkslkifqkimhiklsqsiathi*vxhlht
fihvdmlps*knwlidnpkmvkn

Frame B:
xhtqkhf*iii*tthilhartir*ni*ly*YTIKKHYTANANYCIFQIKKK---

---ysrfr*fpr**yn*rrgssciikk**nnnyyns*qq****frk*s*r***yftxsxi
*yfnhl**ilsnxkslviwi**k*ktietrrnl*ryfrrsct*nchnr*pptfryxicih
ssm*tccrhekig**tirkw*r

Frame C:
xthknifr*lfrphtfythaqyvriynyinir*kniiqqtqiivsfklkkk---

---IPDLDDFQDDNIIEEEDPAVLSKNNKTTTTTTANNNNNNNSENKVEDNDNILRTRTY
DISITYDKYYQTPRVWLFGYDENRKPLKPEEIFEDISEDHAHKTVTIDSHPHLGXSFAYI
HPCRHAAVMKKLVDRQSENGKE
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI445 (VFI445Q) /CSM/VF/VFI4-B/VFI445Q.Seq.d/                        573   e-162
SLB748 (SLB748Q) /CSM/SL/SLB7-B/SLB748Q.Seq.d/                        573   e-162
SFI461 (SFI461Q) /CSM/SF/SFI4-C/SFI461Q.Seq.d/                        573   e-162
SFH850 (SFH850Q) /CSM/SF/SFH8-C/SFH850Q.Seq.d/                        573   e-162
CFH171 (CFH171Q) /CSM/CF/CFH1-C/CFH171Q.Seq.d/                        573   e-162
CFB657 (CFB657Q) /CSM/CF/CFB6-C/CFB657Q.Seq.d/                        573   e-162
AFM817 (AFM817Q) /CSM/AF/AFM8-A/AFM817Q.Seq.d/                        573   e-162
AFI740 (AFI740Q) /CSM/AF/AFI7-B/AFI740Q.Seq.d/                        573   e-162
AFN465 (AFN465Q) /CSM/AF/AFN4-C/AFN465Q.Seq.d/                        571   e-162
AFL613 (AFL613Q) /CSM/AF/AFL6-A/AFL613Q.Seq.d/                        571   e-162
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU582697|BU582697.1 rc67g08.y2 Meloidogyne hapla egg SL1 TOPO v1 Meloidogyne hapla cDNA similar to WP:CE25486 Y55F3AM.4 ;, mRNA sequence.    60   6e-10  2
CK242486|CK242486.1 rx10g04.y1 Meloidogyne paranaensis egg SMART pGEM Meloidogyne paranaensis cDNA 5' similar to TR:Q9VVS6 Q9VVS6 CG6877 PROTEIN. ;, mRNA sequence.    68   2e-07  1
CN254864|CN254864.1 BIP1132 BIP up-regulated phloem library after plant infestation with Myzus persicae Apium graveolens cDNA, mRNA sequence.    68   2e-07  1
BE608405|BE608405.1 sq30e12.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-6983 5' similar to TR:O43035 O43035 HYPOTHETICAL 31.9 KD PROTEIN. ;, mRNA sequence.    48   4e-07  3
BG041867|BG041867.1 saa42b08.y1 Gm-c1059 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1059-1600 5' similar to TR:Q9VVS6 Q9VVS6 CG6877 PROTEIN. ;, mRNA sequence.    48   4e-07  3
BQ612216|BQ612216.1 sap80h04.y1 Gm-c1087 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1087-7255 5' similar to TR:Q9VVS6 Q9VVS6 CG6877 PROTEIN. ;, mRNA sequence.    48   5e-07  3
CA799373|CA799373.1 sat32h02.y1 Gm-c1056 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1056-3411 5' similar to TR:Q9VVS6 Q9VVS6 CG6877 PROTEIN. ;, mRNA sequence.    48   5e-07  3
BI969174|BI969174.1 GM830007B10F05 Gm-r1083 Glycine max cDNA clone Gm-r1083-2577 3', mRNA sequence.    48   8e-07  3
BW391717|BW391717.1 Ciona intestinalis cDNA, clone:ciem806h23, 3'end, single read.    58   8e-06  2
BM891034|BM891034.1 sam22a12.y1 Gm-c1068 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1068-5400 5' similar to TR:Q9VVS6 Q9VVS6 CG6877 PROTEIN. ;, mRNA sequence.    48   2e-05  3
dna update 2004. 9.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU972874_1(EU972874|pid:none) Zea mays clone 389483 autophagy-re...   134   1e-30
BT052164_1(BT052164|pid:none) Medicago truncatula clone MTYF9_FA...   134   1e-30
FJ747649_1(FJ747649|pid:none) Triticum aestivum ATG3 protein (AT...   134   2e-30
AY350711_16(AY350711|pid:none) Sisymbrium irio clone BAC Sio11E1...   134   2e-30
(Q0WWQ1) RecName: Full=Autophagy-related protein 3; AltName: Ful...   133   3e-30
FJ416327_1(FJ416327|pid:none) Bombyx mori autophagy related prot...   132   7e-30
(Q6GQE7) RecName: Full=Autophagy-related protein 3; AltName: Ful...   127   2e-28
(Q0VCL3) RecName: Full=Autophagy-related protein 3;  &BC120110_1...   127   2e-28
(Q9NT62) RecName: Full=Autophagy-related protein 3; AltName: Ful...   127   2e-28
AF175224_1(AF175224|pid:none) Rattus norvegicus preconditioning-...   127   2e-28
protein update 2009. 4.23
PSORT 

 psg: 0.79  gvh: 0.18  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for AFI740 is nuc
5' end seq. ID AFI740F
5' end seq. 
>AFI740F.Seq
ATNACACACACAAAAACATTTTTAGATAATTATTTAGACCACACACATTTTACACGCACG
CACAATACGTTAGAATATATAATTATATTAATATACGATAAAAAAACATTATACAGCAAA
CGCAAATTATTGTATCTTTCAAATTAAAAAAAAAA----------
Length of 5' end seq. 155
3' end seq. ID AFI740Z
3' end seq. 
>AFI740Z.Seq
----------ATATTCCAGATTTAGATGATTTCCAAGATGATAATATAATTGAAGAAGAG
GATCCAGCTGTATTATCAAAAAATAATAAAACAACAACTACTACAACAGCTAACAACAAT
AATAATAATAATTCAGAAAATAAAGTTGAAGATAATGATAATATTTTACGNACTCGNACA
TATGATATTTCAATCACTTATGATAAATATTATCAAACNCCAAGAGTTTGGTTATTTGGA
TATGATGAAAATAGAAAACCATTGAAACCAGAAGAAATCTTTGAAGATATTTCAGAAGAT
CATGCACATAAAACTGTCACAATCGATAGCCACCCACATTTAGGTATNTCATTTGCATAC
ATTCATCCATGTAGACATGCTGCCGTCATGAAAAAATTGGTTGATAGACAATCCGAAAAT
GGTAAAGAAC
Length of 3' end seq. 420
Connected seq. ID AFI740P
Connected seq. 
>AFI740P.Seq
ATNACACACACAAAAACATTTTTAGATAATTATTTAGACCACACACATTTTACACGCACG
CACAATACGTTAGAATATATAATTATATTAATATACGATAAAAAAACATTATACAGCAAA
CGCAAATTATTGTATCTTTCAAATTAAAAAAAAAA----------ATATTCCAGATTTAG
ATGATTTCCAAGATGATAATATAATTGAAGAAGAGGATCCAGCTGTATTATCAAAAAATA
ATAAAACAACAACTACTACAACAGCTAACAACAATAATAATAATAATTCAGAAAATAAAG
TTGAAGATAATGATAATATTTTACGNACTCGNACATATGATATTTCAATCACTTATGATA
AATATTATCAAACNCCAAGAGTTTGGTTATTTGGATATGATGAAAATAGAAAACCATTGA
AACCAGAAGAAATCTTTGAAGATATTTCAGAAGATCATGCACATAAAACTGTCACAATCG
ATAGCCACCCACATTTAGGTATNTCATTTGCATACATTCATCCATGTAGACATGCTGCCG
TCATGAAAAAATTGGTTGATAGACAATCCGAAAATGGTAAAGAAC
Length of connected seq. 575
Full length Seq ID -
Full length Seq. -
Length of full length seq. -