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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHA443
Library SH
(Link to library)
Clone ID SHA443
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16429-1
Original site URL
Representative seq. ID SHA443P
(Link to Original site)
Representative DNA sequence 
>SHA443 (SHA443Q) /CSM/SH/SHA4-B/SHA443Q.Seq.d/
AAAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTANAATTCTTTTGGAATC
AGCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAGGATGTTGAAAATATCTTAAA
TTGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAXXXXXXXXXXTTTCAAAGAGAC
CTGCCATTACAAATGCTTATTGTCTTTTGAATCTTGGTGATAGTATTACCACTGATCATA
TTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAG
GTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGG
TTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCAC
AANCAACNTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATC
AATCAGAAGGTCATCAACTCATTGTTTTGGNTGGTAGTGATTATGGCTNTGGTAGTTNTA
GAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCANTGCCATNTCAT
TTGAANGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAAC
CAGGTCAANAT
sequence update 2002.10.25
Translated Amino Acid sequence 
KLQDPRIEKLPYSIXILLESAVRNCDNFEVHEKDVENILNWENTANKVEIPF---

---SKRPAITNAYCLLNLGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRR
GNDEIMVRGTFANTRIVNKLAPAVGPQXTYVPTGELMFISDAAEKYQSEGHQLIVLXGSD
YGXGSXRDWAAKGPYLQGIKCVXAXSFEXIHRSNLVGMGIIPLQFQPGQX
Translated Amino Acid sequence (All Frames) 
Frame A:
ktsrpkn*kitilhxnsfgisss*l**l*strkgc*kylklgkhck*s*nsf---

---fqrdlplqmlivf*ilvivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlv
evmmklwlevplpilvllinwlqplvhxqxmyqlvs*clfqmlqkninqkvinslfwxvv
imaxvvxeiglpkvhtfkelsvsxpxhlxvfidpislvwvlshcnsnqvx

Frame B:
KLQDPRIEKLPYSIXILLESAVRNCDNFEVHEKDVENILNWENTANKVEIPF---

---fketchykcllsfesw**yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*
r***nyg*rylcqysyc**igssrwstxnxctnw*vnvyfrccrkisirrssthcfgw**
lwlw*x*rlgcqrsipsrn*vcxchxi*xys*iqsrwygyypiaiptrsx

Frame C:
nfktqelknyhtplxffwnqqfvivitlkytkrmlkis*igktlqiklkfls---

---SKRPAITNAYCLLNLGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRR
GNDEIMVRGTFANTRIVNKLAPAVGPQXTYVPTGELMFISDAAEKYQSEGHQLIVLXGSD
YGXGSXRDWAAKGPYLQGIKCVXAXSFEXIHRSNLVGMGIIPLQFQPGQX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHA443 (SHA443Q) /CSM/SH/SHA4-B/SHA443Q.Seq.d/                       1211   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                        944   0.0
VHB318 (VHB318Q) /CSM/VH/VHB3-A/VHB318Q.Seq.d/                        944   0.0
VHA714 (VHA714Q) /CSM/VH/VHA7-A/VHA714Q.Seq.d/                        944   0.0
VHA182 (VHA182Q) /CSM/VH/VHA1-D/VHA182Q.Seq.d/                        944   0.0
SHK673 (SHK673Q) /CSM/SH/SHK6-D/SHK673Q.Seq.d/                        944   0.0
SHG655 (SHG655Q) /CSM/SH/SHG6-C/SHG655Q.Seq.d/                        944   0.0
SHG376 (SHG376Q) /CSM/SH/SHG3-D/SHG376Q.Seq.d/                        944   0.0
SHG304 (SHG304Q) /CSM/SH/SHG3-A/SHG304Q.Seq.d/                        944   0.0
SHE776 (SHE776Q) /CSM/SH/SHE7-D/SHE776Q.Seq.d/                        944   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    70   3e-24  4
BQ511380|BQ511380.2 EST618795 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMHR19 5' end, mRNA sequence.    58   1e-16  3
BI927352|BI927352.1 EST547241 tomato flower, 3 - 8 mm buds Lycopersicon esculentum cDNA clone cTOB22E7 5' end, mRNA sequence.    58   2e-15  3
AW622039|AW622039.1 EST312837 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX14G21 5', mRNA sequence.    58   3e-15  3
CK719688|CK719688.1 19969 Swollen Stolon Solanum tuberosum cDNA, mRNA sequence.    58   3e-15  3
BG889764|BG889764.1 EST515615 cSTD Solanum tuberosum cDNA clone cSTD15K20 5' sequence, mRNA sequence.    58   3e-15  3
AI775579|AI775579.1 EST256679 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER15P4, mRNA sequence.    58   6e-14  2
DT601625|DT601625.1 ram01-4ms1-c11 Ram01 Ribes americanum cDNA clone ram01-4ms1-c11 5', mRNA sequence.    48   5e-13  3
AW455290|AW455290.1 EST311828 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX10C9 5', mRNA sequence.    58   9e-13  2
BM062502|BM062502.1 KS01043F01 KS01 Capsicum annuum cDNA, mRNA sequence.    58   1e-12  2
dna update 2005.12. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   322   8e-87
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   224   1e-57
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   224   1e-57
BC043991_1(BC043991|pid:none) Xenopus laevis aconitase 1, mRNA (...   224   2e-57
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   224   2e-57
BC168529_1(BC168529|pid:none) Xenopus tropicalis cDNA clone MGC:...   223   3e-57
(Q01059) RecName: Full=Cytoplasmic aconitate hydratase;         ...   222   9e-57
EU816775_1(EU816775|pid:none) Perkinsus marinus aconitase-1 mRNA...   222   9e-57
(Q90875) RecName: Full=Cytoplasmic aconitate hydratase;         ...   221   1e-56
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   220   3e-56
protein update 2009. 4.14
PSORT 

 psg: 0.87  gvh: 0.39  alm: 0.50  top: 0.33  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for SHA443 is cyt
5' end seq. ID SHA443F
5' end seq. 
>SHA443F.Seq
AAAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTANAATTCTTTTGGAATC
AGCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAGGATGTTGAAAATATCTTAAA
TTGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCANNNNNNNNNN
Length of 5' end seq. 168
3' end seq. ID SHA443Z
3' end seq. 
>SHA443Z.Seq
NNNNNNNNNNTTTCAAAGAGACCTGCCATTACAAATGCTTATTGTCTTTTGAATCTTGGT
GATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCC
GCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGT
AGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAA
TTGGCTCCAGCCGTTGGTCCACAANCAACNTATGTACCAACTGGTGAGTTAATGTTTATT
TCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGNTGGTAGT
GATTATGGCTNTGGTAGTTNTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATT
AAGTGTGTCANTGCCATNTCATTTGAANGTATTCATAGATCCAATCTCGTTGGTATGGGT
ATTATCCCATTGCAATTCCAACCAGGTCAANAT
Length of 3' end seq. 513
Connected seq. ID SHA443P
Connected seq. 
>SHA443P.Seq
AAAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTANAATTCTTTTGGAATC
AGCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAGGATGTTGAAAATATCTTAAA
TTGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCA----------TTTCAAAGAGAC
CTGCCATTACAAATGCTTATTGTCTTTTGAATCTTGGTGATAGTATTACCACTGATCATA
TTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAG
GTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGG
TTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCAC
AANCAACNTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATC
AATCAGAAGGTCATCAACTCATTGTTTTGGNTGGTAGTGATTATGGCTNTGGTAGTTNTA
GAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCANTGCCATNTCAT
TTGAANGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAAC
CAGGTCAANAT
Length of connected seq. 661
Full length Seq ID -
Full length Seq. -
Length of full length seq. -