>AFI709 (AFI709Q) /CSM/AF/AFI7-A/AFI709Q.Seq.d/
ACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAGTATCAGGTAATACA
CCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAGAAAAAGATAGACAA
TTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAGCAGTTATGGAAGCA
TTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTTCAAGATATTATGGT
GGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAGCATTAAAAGCATTC
CGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGTGGTAGTCCAGCCAAT
TTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGGGTTTAGATTTACCA
TCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGATTTCAGCATCATCA
ATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAAXXXXXXXXXXGCTG
CTCAATTATTGGATTCACCATTCGATTATTGTGATGTTGTCACCAGTACCACTCATAAAA
CCTTAAGAGGACCACGTTCCAGGAATCATCTTCTTTAGAAGAGGTAAAAGAGTCGATGGT
AATGGTAAAGAAATTGAAGAGTACGACATTGAATCAAAGATCAATTTCGCAGTTTTCCCA
TCACTCCAAGGTGGTCCACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCTCAAAGAA
GCCGACTCTCAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGCTGCCATT
GGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGACAATCATTTA
ATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGCTGCCGAT
ATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGCAATTTCACCA
GGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGCCGATTTT
GAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCAAGGTCGT
GTCGGTAAGAAATTGGTTGATTTTGTTGTTGAAATTAATAAATCAAAAGAATTATTAGAT
TTACGTAAAGAAGTTGAAGAATTCTCTTCAAAATTCACTTTACCAGGTATTTAAATAATA
AGGTAAAAAAC

tqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEA
LGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPAN
FAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGL---

---AAQLLDSPFDYCDVVTSTTHKTLRGPRSRNHLL*kr*ksrw*w*rn*rvrh*ikdqf
rsfpitprwst*kcycwccccsqrsrlsriqrvcpss*kecrchw*csheqrl*igykwy
*qsfnslgfktkgthw**i*kscryr*yhrq*kcrpw*yqcnftrwysywficshk*rfk
gsrf*kdc*fp**nrfnls*nsrscr*eig*fcc*n**ikriirft*rs*rilfkihftr
ylnnkvkn

Frame A:
tqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEA
LGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPAN
FAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGL---

---AAQLLDSPFDYCDVVTSTTHKTLRGPRSRNHLL*kr*ksrw*w*rn*rvrh*ikdqf
rsfpitprwst*kcycwccccsqrsrlsriqrvcpss*kecrchw*csheqrl*igykwy
*qsfnslgfktkgthw**i*kscryr*yhrq*kcrpw*yqcnftrwysywficshk*rfk
gsrf*kdc*fp**nrfnls*nsrscr*eig*fcc*n**ikriirft*rs*rilfkihftr
ylnnkvkn

Frame B:
hk*lynihihikwvqyqvihh*kklimksln**iekkidnlkv*n*lplkilhqeqlwkh
*dhilqinmqkviqvqdimvvqkllmn*klfvkreh*khsvwmrvngvlmfnhtvvvqpi
sqftqhy*dhtiv*wv*iyhqvvi*lmvikpirrrfqhhqsfsnqchiklvlmv*---

---llnywihhsiivmlspvplikp*edhvpgiiffrrgkrvdgngkeieeydieskinf
avfpslqggphenviagvavalkeadsqefkeyalqvkknaaaignalmnkgyklvtngt
dnhlilwdlrpkeltgnkfekaadianitvnknavhgdtnaispggirigssaltsrglk
eadfekiadfldrivsisleiqgrvgkklvdfvveinkskelldlrkeveefsskftlpg
i*iir*k

Frame C:
tsnyiiyiyi*ngfsir*ytikrs***nl*ine*rkr*ti*rfrincl*kfyikssygsi
ritfyk*icrrlsrfkilwwyrsc**irnslskesiksipfg*e*mgc*cstiqw*ssqf
rslhsiikttrsyngfrftirwsfnswlsnr*eedfsiinlfrinaisnwc*wf---

---csiigftirll*cchqyhs*nlkrttfqesssleevkesmvmvkklksttlnqrsis
qfshhskvvhmkmlllvllllskkptlknsksmpfklkrmplplvmls*tkvinwlqmvl
tii*ffgi*dqrnslvinlkklpislispsikmpsmvipmqfhqvvfvlvhllsqvev*r
kpilkrllisliesfqsllkfkvvsvrnwlilllklinqkny*iyvkklknslqnslyqv
fk**gkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI709 (AFI709Q) /CSM/AF/AFI7-A/AFI709Q.Seq.d/                       2460   0.0
VFG867 (VFG867Q) /CSM/VF/VFG8-C/VFG867Q.Seq.d/                       1441   0.0
VFC539 (VFC539Q) /CSM/VF/VFC5-B/VFC539Q.Seq.d/                       1441   0.0
SFD348 (SFD348Q) /CSM/SF/SFD3-B/SFD348Q.Seq.d/                       1441   0.0
SFB690 (SFB690Q) /CSM/SF/SFB6-D/SFB690Q.Seq.d/                       1441   0.0
CFJ182 (CFJ182Q) /CSM/CF/CFJ1-D/CFJ182Q.Seq.d/                       1441   0.0
CFF296 (CFF296Q) /CSM/CF/CFF2-D/CFF296Q.Seq.d/                       1441   0.0
CFC879 (CFC879Q) /CSM/CF/CFC8-D/CFC879Q.Seq.d/                       1441   0.0
CFC685 (CFC685Q) /CSM/CF/CFC6-D/CFC685Q.Seq.d/                       1441   0.0
AFN802 (AFN802Q) /CSM/AF/AFN8-A/AFN802Q.Seq.d/                       1441   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF009966|AF009966.1 Candida albicans serine hydroxymethyl transferase II (SHMII) gene, complete cds.    58   2e-20  6
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    58   5e-17  20
BI418085|BI418085.1 LjNEST24d6r Lotus japonicus nodule library 5 and 7 week-old Lotus japonicus cDNA 5', mRNA sequence.    60   2e-13  3
CB408430|CB408430.1 IPG006D12_249762 JH III-treated male I. pini midguts Ips pini cDNA clone IPG006D12 5, mRNA sequence.    62   3e-12  3
BP187516|BP187516.1 Dugesia japonica cDNA, clone: 00331_HN, expressed in the head.    52   7e-12  3
AF009965|AF009965.1 Candida albicans serine hydroxymethyl-transferase I (SHM1) gene, complete cds.    38   9e-12  7
CV017773|CV017773.1 tbt_009554 Normalized Nicotiana tabacum cDNA library Nicotiana tabacum cDNA clone tbt_009554 5', mRNA sequence.    66   9e-12  2
BQ489771|BQ489771.1 14-E9425-006-009-K03-T3 Sugar beet MPIZ-ADIS-006 Lambda Zap II library Beta vulgaris cDNA clone K-3-9, mRNA sequence.    50   9e-12  3
AL408772|AL408772.1 T7 end of clone AV0AA010F02 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    58   1e-10  3
AI960890|AI960890.1 sc92a10.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-787 5' similar to TR:O04952 O04952 SERINE HYDROXYMETHYLTRANSFERASE ;, mRNA sequence.    52   1e-10  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT045792_1(BT045792|pid:none) Salmo salar clone ssal-rgf-531-152...   399   e-110
(Q9SZJ5) RecName: Full=Serine hydroxymethyltransferase, mitochon...   384   e-105
CR954219_30(CR954219|pid:none) Ostreococcus tauri strain OTTH059...   382   e-104
CT005253_136(CT005253|pid:none) Leishmania major strain Friedlin...   379   e-104
CS497796_1(CS497796|pid:none) Sequence 69 from Patent WO20070117...   376   e-102
AC117988_17(AC117988|pid:none) Oryza sativa chromosome 3 BAC OSJ...   374   e-102
CP000596_241(CP000596|pid:none) Ostreococcus lucimarinus CCE9901...   370   e-101
BC022874_1(BC022874|pid:none) Homo sapiens serine hydroxymethylt...   363   9e-99
AY035117_1(AY035117|pid:none) Arabidopsis thaliana putative glyc...   353   7e-96
(P34899) RecName: Full=Serine hydroxymethyltransferase, mitochon...   351   3e-95

 psg: 0.81  gvh: 0.40  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for AFI709 is cyt

>AFI709F.Seq
ACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAGTATCAGGTAATACA
CCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAGAAAAAGATAGACAA
TTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAGCAGTTATGGAAGCA
TTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTTCAAGATATTATGGT
GGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAGCATTAAAAGCATTC
CGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGTGGTAGTCCAGCCAAT
TTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGGGTTTAGATTTACCA
TCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGATTTCAGCATCATCA
ATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAA----------

>AFI709Z.Seq
----------GCTGCTCAATTATTGGATTCACCATTCGATTATTGTGATGTTGTCACCAG
TACCACTCATAAAACCTTAAGAGGACCACGTTCCAGGAATCATCTTCTTTAGAAGAGGTA
AAAGAGTCGATGGTAATGGTAAAGAAATTGAAGAGTACGACATTGAATCAAAGATCAATT
TCGCAGTTTTCCCATCACTCCAAGGTGGTCCACATGAAAATGTTATTGCTGGTGTTGCTG
TTGCTCTCAAAGAAGCCGACTCTCAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGA
ATGCCGCTGCCATTGGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTA
CTGACAATCATTTAATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTG
AAAAAGCTGCCGATATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCA
ATGCAATTTCACCAGGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAA
AGGAAGCCGATTTTGAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTG
AAATTCAAGGTCGTGTCGGTAAGAAATTGGTTGATTTTGTTGTTGAAATTAATAAATCAA
AAGAATTATTAGATTTACGTAAAGAAGTTGAAGAATTCTCTTCAAAATTCACTTTACCAG
GTATTTAAATAATAAGGTAAAAAAC

>AFI709P.Seq
ACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAGTATCAGGTAATACA
CCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAGAAAAAGATAGACAA
TTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAGCAGTTATGGAAGCA
TTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTTCAAGATATTATGGT
GGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAGCATTAAAAGCATTC
CGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGTGGTAGTCCAGCCAAT
TTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGGGTTTAGATTTACCA
TCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGATTTCAGCATCATCA
ATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAA----------GCTG
CTCAATTATTGGATTCACCATTCGATTATTGTGATGTTGTCACCAGTACCACTCATAAAA
CCTTAAGAGGACCACGTTCCAGGAATCATCTTCTTTAGAAGAGGTAAAAGAGTCGATGGT
AATGGTAAAGAAATTGAAGAGTACGACATTGAATCAAAGATCAATTTCGCAGTTTTCCCA
TCACTCCAAGGTGGTCCACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCTCAAAGAA
GCCGACTCTCAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGCTGCCATT
GGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGACAATCATTTA
ATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGCTGCCGAT
ATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGCAATTTCACCA
GGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGCCGATTTT
GAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCAAGGTCGT
GTCGGTAAGAAATTGGTTGATTTTGTTGTTGAAATTAATAAATCAAAAGAATTATTAGAT
TTACGTAAAGAAGTTGAAGAATTCTCTTCAAAATTCACTTTACCAGGTATTTAAATAATA
AGGTAAAAAAC