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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHA307
Library SH
(Link to library)
Clone ID SHA307
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16439-1
Original site URL
Representative seq. ID SHA307P
(Link to Original site)
Representative DNA sequence 
>SHA307 (SHA307Q) /CSM/SH/SHA3-A/SHA307Q.Seq.d/
TGTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTTTTTAATATAT
TTTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAAAAAAATGTCT
AAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAGCAGACAACTT
TATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTTAGTTGTAGGT
TTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTT
ACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCA
CCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTA
AATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAA
TTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTT
TGTCATGCAAATAAAATTCATTTCATTAXXXXXXXXXXATCCAAAANCAAGCTGCCAATG
TTATTGTTCCAGATTTCACTCCAAAGAAGGTTAAGATTCAAACNTCTGAAAATGAACCAG
CTCCCATCTTCAAACACTCAACAAGCTGGTGGTGATGCTGAAGATGATCAATGTGATACC
ATTTTATCACAACTTCCACAACCAAGTGAAATGGCTGGTTACAAAATTAACTCTATTCAA
TTTGAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACCTCAAATTTA
AGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGT
AAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAA
TTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTA
GGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAA
GGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAG
TTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTT
ACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAA
ATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAGAAATATTTA
TTACTGAAATTTGTTGCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
cl*lqitififlyllffliyffififlyklt*KDKKKKMSKPMDVEQEPKIDDALYSRQL
YALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQFYFS
PEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKVNEF
CHANKIHFI---

---SKXKLPMLLFQISLQRRLRFKXLKMNQLPSSNTQQAGGDAEDDQCDTILSQLPQPSE
MAGYKINSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTT
AVVAGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRF
DVDGDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSK
KPLPKDKKYLLLKFVA
Translated Amino Acid sequence (All Frames) 
Frame A:
cl*lqitififlyllffliyffififlyklt*KDKKKKMSKPMDVEQEPKIDDALYSRQL
YALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQFYFS
PEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKVNEF
CHANKIHFI---

---iqxqaanvivpdftpkkvkiqtsenepapifkhstsww*c*r*sm*yhfittsttk*
ngwlqn*lysi*kg**yqssyrfhyrylkfksyqlcnqss**t*dqryrr*nhssfsyhy
crscwfrlyrid*sysk*sfgei*ehlyefrypilwfr*tncrpkeqns*rldldtlgsf
rc*w*yhsqgvfrsl*ketwfgyfnall*sytficslh**en*rgkikdenlttl*nplk
etitkr*eifiteicc

Frame B:
vynyk*lflyfyiyyff*yiflfsffcinlhkkikrkkclnqwm*nknqklmmlyiadnf
mhylmkq*kklhqlqf*l*vykvlvlkllki*v*qvlnllhcmikn*lklkicllnfifh
qnklvklveqmhvskkl*i*iimlelmyimen*vmns*rnsml*y*pinh*hynsk*mnf
vmqikfisl---

---SKXKLPMLLFQISLQRRLRFKXLKMNQLPSSNTQQAGGDAEDDQCDTILSQLPQPSE
MAGYKINSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTT
AVVAGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRF
DVDGDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSK
KPLPKDKKYLLLKFVA

Frame C:
fiitnnyfyififiiffniffyfhffv*tyikr*keknv*tngcrtrtkn**cfi*qttl
ciis*nneknyinfsfscrftrswy*nc*rfkfsrc*icyiv**ris*n*rfvfsilfft
rtsw*sw*srcmfpkscrfk*lc*n*ct*wrik**ilkeiqccsisqstisittqse*il
sck*nsfh---

---pkxscqcycsrfhskeg*dsnx*k*tsshlqtlnklvvmlkminvipfyhnfhnqvk
wlvtkltlfnlkrmmipiiisislplpqi*elptmqsvqlinirpkvspvksfql*lpll
p*llvsfvsn*lklfkiklwrnirapl*i*vshslvslnqlppqrtkfvkaglghfgivs
mlmvislsrsf*islkrnmvwifqcslvklhfymlsslirklkrkd*r*kshnsmkpsqr
nhyqkirniyy*nllq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHA307 (SHA307Q) /CSM/SH/SHA3-A/SHA307Q.Seq.d/                       2313   0.0
SHA779 (SHA779Q) /CSM/SH/SHA7-D/SHA779Q.Seq.d/                       2264   0.0
VHA132 (VHA132Q) /CSM/VH/VHA1-B/VHA132Q.Seq.d/                       1475   0.0
SHD220 (SHD220Q) /CSM/SH/SHD2-A/SHD220Q.Seq.d/                       1475   0.0
CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS124Q.Seq.d/                       1473   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1469   0.0
AHQ886 (AHQ886Q) /CSM/AH/AHQ8-D/AHQ886Q.Seq.d/                       1469   0.0
AHN324 (AHN324Q) /CSM/AH/AHN3-A/AHN324Q.Seq.d/                       1467   0.0
VHA328 (VHA328Q) /CSM/VH/VHA3-B/VHA328Q.Seq.d/                       1465   0.0
SHC572 (SHC572Q) /CSM/SH/SHC5-C/SHC572Q.Seq.d/                       1459   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CB933531|CB933531.1 ri31b11.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodisequence.    64   4e-12  2
AZ930776|AZ930776.1 474.dhz58e03.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz58e03.s1, DNA sequence.    68   1e-11  2
DR174736|DR174736.1 HR0003_M02:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   3e-06  2
DR174741|DR174741.1 HR0003_M08:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   3e-06  2
DR174755|DR174755.1 HR0003_N01:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   4e-06  2
AC134210|AC134210.1 Rattus norvegicus clone CH230-197N6, *** SEQUENCING IN PROGRESS ***, 60 unordered pieces.    58   5e-04  1
AC108277|AC108277.6 Rattus norvegicus clone CH230-27E16, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    58   5e-04  1
BZ303839|BZ303839.1 KD2618.q1 Kluyveromyces delphensis Random Genomic Library Kluyveromyces delphensis genomic clone KD2618, DNA sequence.    48   0.002  2
DR028930|DR028930.1 bda010063_A05_11.b1_024.ab1 Antrodia cinnamomea cDNA Library Antrodia cinnamomea cDNA, mRNA sequence.    52   0.003  2
BH154952|BH154952.1 ENTTP11TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   0.011  2
dna update 2005.12. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   419   e-115
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   233   1e-59
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   220   3e-58
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   220   7e-56
AE016819_666(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   208   6e-55
AM270347_41(AM270347|pid:none) Aspergillus niger contig An15c020...   203   2e-54
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   214   5e-54
CR382122_592(CR382122|pid:none) Kluyveromyces lactis strain NRRL...   205   6e-54
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   212   2e-53
BX908809_46(BX908809|pid:none) Neurospora crassa DNA linkage gro...   210   8e-53
protein update 2009. 4.14
PSORT 

 psg: 0.50  gvh: 0.52  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.21  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  8.0 %: peroxisomal
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for SHA307 is cyt
5' end seq. ID SHA307F
5' end seq. 
>SHA307F.Seq
TGTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTTTTTAATATAT
TTTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAAAAAAATGTCT
AAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAGCAGACAACTT
TATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTTAGTTGTAGGT
TTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTT
ACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCA
CCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTA
AATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAA
TTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTT
TGTCATGCAAATAAAATTCATTTCATTANNNNNNNNNN
Length of 5' end seq. 578
3' end seq. ID SHA307Z
3' end seq. 
>SHA307Z.Seq
NNNNNNNNNNATCCAAAANCAAGCTGCCAATGTTATTGTTCCAGATTTCACTCCAAAGAA
GGTTAAGATTCAAACNTCTGAAAATGAACCAGCTCCCATCTTCAAACACTCAACAAGCTG
GTGGTGATGCTGAAGATGATCAATGTGATACCATTTTATCACAACTTCCACAACCAAGTG
AAATGGCTGGTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATC
ATATCGATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAG
CTGATAAACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTA
CTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTT
TGGAGAAATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAAC
CAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTT
TCGATGTTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATG
GTTTGGATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATA
AGAAAACTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAA
AGAAACCATTACCAAAAGATAAGAAATATTTATTACTGAAATTTGTTGCAA
Length of 3' end seq. 771
Connected seq. ID SHA307P
Connected seq. 
>SHA307P.Seq
TGTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTTTTTAATATAT
TTTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAAAAAAATGTCT
AAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAGCAGACAACTT
TATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTTAGTTGTAGGT
TTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTT
ACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCA
CCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTA
AATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAA
TTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTT
TGTCATGCAAATAAAATTCATTTCATTA----------ATCCAAAANCAAGCTGCCAATG
TTATTGTTCCAGATTTCACTCCAAAGAAGGTTAAGATTCAAACNTCTGAAAATGAACCAG
CTCCCATCTTCAAACACTCAACAAGCTGGTGGTGATGCTGAAGATGATCAATGTGATACC
ATTTTATCACAACTTCCACAACCAAGTGAAATGGCTGGTTACAAAATTAACTCTATTCAA
TTTGAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACCTCAAATTTA
AGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGT
AAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAA
TTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTA
GGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAA
GGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAG
TTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTT
ACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAA
ATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAGAAATATTTA
TTACTGAAATTTGTTGCAA
Length of connected seq. 1329
Full length Seq ID -
Full length Seq. -
Length of full length seq. -