>AFI703 (AFI703Q) /CSM/AF/AFI7-A/AFI703Q.Seq.d/
XXXXXXXXXXCAATGTACGTTATGGGTGTCAATGAAGAAACCTATGAATCATCACACGAC
GTCATTTCAAATGCATCATGTACCACCAATTGTTTAGCTCCATTAGCTAAAATTATCCAC
GAAAACTTTGGTATTGTTGAAGGTTTAATGACCACCGTTCACGCCATCACTGCCACCCAA
AAGACTGTTGATGGTCCATCTGGTAAAGATTGGAGAGCTGGTAGATCTGCCCTTTCAAAC
ATTATCCCAGCCTCAACTGGTGCCGCCAAAGCTGTCGGTAAAGTTTTACCAGCACTCAAT
GGTAAATTAACCGGTATGTCATTCCGTGTTCCAAATTGTGATGTTTCAGTCGTCGATTTA
ACCGTCCGTCTTGAAAAGAAAGCCACCTATGAAGAAATCAAAAAGGTCATGAAAGCTGCC
TCTGAATCTGACAAATACAAGAGATACATTGGTTACACTGAAGATGAAGTTGTCTCAACT
GACTTTATTGGTGATACTCACTCTTCAATTTTCGATGCTCATGCTGGTATCGCCTTAAAT
GATAACTTTGTTAAATTAGTCTCATGGTATGATAATGAAATGGGT

---MYVMGVNEETYESSHDVISNASCTTNCLAPLAKIIHENFGIVEGLMTTVHAITATQK
TVDGPSGKDWRAGRSALSNIIPASTGAAKAVGKVLPALNGKLTGMSFRVPNCDVSVVDLT
VRLEKKATYEEIKKVMKAASESDKYKRYIGYTEDEVVSTDFIGDTHSSIFDAHAGIALND
NFVKLVSWYDNEMG

Frame A:
---nvrygcq*rnl*iitrrhfkcimyhqlfssis*nyprklwyc*rfndhrsrhhchpk
dc*wsiw*rlesw*icpfkhypslnwcrqscr*sftstqw*inryvipcskl*cfsrrfn
rps*keshl*rnqkghescl*i*qiqeihwlh*r*scln*lyw*yslfnfrcscwyrlk*
*lc*islmv***ng


Frame B:
---MYVMGVNEETYESSHDVISNASCTTNCLAPLAKIIHENFGIVEGLMTTVHAITATQK
TVDGPSGKDWRAGRSALSNIIPASTGAAKAVGKVLPALNGKLTGMSFRVPNCDVSVVDLT
VRLEKKATYEEIKKVMKAASESDKYKRYIGYTEDEVVSTDFIGDTHSSIFDAHAGIALND
NFVKLVSWYDNEMG


Frame C:
---ctlwvsmkkpmnhhttsfqmhhvppiv*lh*lklstktlvllkv**ppftpslppkr
llmvhlvkigelvdlpfqtlsqpqlvppklsvkfyqhsmvn*pvchsvfqivmfqssi*p
svlkrkppmkkskrs*klplnltntrdtlvtlkmklsqltllviltlqfsmlmlvsp*mi
tlln*shgmimkw

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK382 (VSK382Q) /CSM/VS/VSK3-D/VSK382Q.Seq.d/                       1140   0.0
VFO172 (VFO172Q) /CSM/VF/VFO1-C/VFO172Q.Seq.d/                       1140   0.0
VFL807 (VFL807Q) /CSM/VF/VFL8-A/VFL807Q.Seq.d/                       1140   0.0
VFG415 (VFG415Q) /CSM/VF/VFG4-A/VFG415Q.Seq.d/                       1140   0.0
VFD893 (VFD893Q) /CSM/VF/VFD8-D/VFD893Q.Seq.d/                       1140   0.0
VFC590 (VFC590Q) /CSM/VF/VFC5-D/VFC590Q.Seq.d/                       1140   0.0
VFB773 (VFB773Q) /CSM/VF/VFB7-D/VFB773Q.Seq.d/                       1140   0.0
SLG754 (SLG754Q) /CSM/SL/SLG7-C/SLG754Q.Seq.d/                       1140   0.0
SLE568 (SLE568Q) /CSM/SL/SLE5-C/SLE568Q.Seq.d/                       1140   0.0
SLB229 (SLB229Q) /CSM/SL/SLB2-B/SLB229Q.Seq.d/                       1140   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1140   0.0    1
U55243|U55243.1 Dictyostelium discoideum glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, partial cds.  1098   0.0    1
BD218177|BD218177.1 Plasmid for gene expression in pichia ciferri and transformation method using the same.   137   2e-43  3
AR411837|AR411837.1 Sequence 5 from patent US 6638735.            137   2e-43  3
AF053300|AF053300.1 Pichia ciferrii glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene, complete cds.   137   2e-43  3
BI816321|BI816321.1 PfESToaa39a10.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:O96369 O96369 GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ;, mRNA sequence.    78   4e-43  5
BI815601|BI815601.1 PfESToaa30e06.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:O96369 O96369 GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ;, mRNA sequence.    78   4e-43  5
AF479765|AF479765.1 Plasmodium falciparum HB3 glyceraldehyde-3-phosphate dehydrogenase mRNA, complete cds.    78   4e-42  5
AF348158|AF348158.1 Plasmodium falciparum strain FCC-1/HN glyceraldehyde-3-phosphate dehydrogenase mRNA, complete cds.    78   5e-42  5
AF030440|AF030440.1 Plasmodium falciparum glyceraldehyde-3-phosphate dehydrogenase mRNA, complete cds.    78   1e-41  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94469) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   383   e-105
U55243_1(U55243|pid:none) Dictyostelium discoideum glyceraldehyd...   365   e-100
AJ246023_1(AJ246023|pid:none) Marchantia polymorpha mRNA for gly...   304   1e-81
DQ873395_1(DQ873395|pid:none) Marchantia polymorpha cytosolic gl...   301   8e-81
BT051304_1(BT051304|pid:none) Medicago truncatula clone MTYF1_F2...   300   1e-80
(P34922) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase,...   300   1e-80
(Q8NK47) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   298   5e-80
(P25861) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase,...   298   5e-80
JQ1287(JQ1287;JS0614) glyceraldehyde-3-phosphate dehydrogenase (...   298   7e-80
BT052789_1(BT052789|pid:none) Medicago truncatula clone MTYFH_FI...   298   7e-80

 psg: 0.75  gvh: 0.54  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for AFI703 is cyt

>AFI703Z.Seq
----------CAATGTACGTTATGGGTGTCAATGAAGAAACCTATGAATCATCACACGAC
GTCATTTCAAATGCATCATGTACCACCAATTGTTTAGCTCCATTAGCTAAAATTATCCAC
GAAAACTTTGGTATTGTTGAAGGTTTAATGACCACCGTTCACGCCATCACTGCCACCCAA
AAGACTGTTGATGGTCCATCTGGTAAAGATTGGAGAGCTGGTAGATCTGCCCTTTCAAAC
ATTATCCCAGCCTCAACTGGTGCCGCCAAAGCTGTCGGTAAAGTTTTACCAGCACTCAAT
GGTAAATTAACCGGTATGTCATTCCGTGTTCCAAATTGTGATGTTTCAGTCGTCGATTTA
ACCGTCCGTCTTGAAAAGAAAGCCACCTATGAAGAAATCAAAAAGGTCATGAAAGCTGCC
TCTGAATCTGACAAATACAAGAGATACATTGGTTACACTGAAGATGAAGTTGTCTCAACT
GACTTTATTGGTGATACTCACTCTTCAATTTTCGATGCTCATGCTGGTATCGCCTTAAAT
GATAACTTTGTTAAATTAGTCTCATGGTATGATAATGAAATGGGT