>SHA256 (SHA256Q) /CSM/SH/SHA2-C/SHA256Q.Seq.d/
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTGCATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAA
ANGGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCANA
AATATGTCTGTTATNGCTCATGTNGATCATGGTAAAACTACTCTTACTGATTCATTAATT
CAAAGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGT
CCANATGAACAATTACGNGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAA
ANGCCAAAAGXXXXXXXXXXTCTACATGAAATCTGAACCCATTATCGTCAGACCTAACTG
ATGATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATC
TCTATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAG
AATATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGGTGTTCAATATCTTCATGAA
ATTAAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGT
GAGAAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATT
CATAGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTA
TCAGCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGT
GGTATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAA
CGTCGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTT
GGTCTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTT
GATCATTGGAGTTCAATTGGTATCGTTGGTCAAGANAAAAAAA

visiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*NKKK
XVNFTIDQIRSIMDRRENIXNMSVXAHVDHGKTTLTDSLIQRAGIIAXKVSGNAGYMSCR
PXEQLRGITIKSSSVSLHFEXPK---

---YMKSEPIIVRPN**y**wfggf*src*kscqlsl**tflg*e*cs*hlviwsri*gs
kcfs*qnqrvfnifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cfmpmqfievv
vnlfqqlvv*cmppnyqlnqhfwnqciwlilvllkvvlvvstlysiveevlslvknvvlv
lhysisklifl*wnhlvslliyvqvllvklslnvfliigvqlvslvkxkk

Frame A:
visiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*NKKK
XVNFTIDQIRSIMDRRENIXNMSVXAHVDHGKTTLTDSLIQRAGIIAXKVSGNAGYMSCR
PXEQLRGITIKSSSVSLHFEXPK---

---st*nlnplssdltddidnglvafnqdvksranylydkhswdkndavniwsfgpeykg
pnvlvnktkgcsiss*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*
wsiysnnsscnvclptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsyw
ysiiqyqslsscngiiwshc*ftfkycwssfpsmcf*slefnwyrwsrxk

Frame B:
syq**illhiqnkinkt*tlffpcigqtliclctiilfkllllkfilktliskktktkkk
xsilq*ikfvqlwivxkisxicllxlmximvkllllih*fkelvllxirylvmqdichvv
xmnnyxvlp*shhlfhftlkxqk---

---lhei*thyrqt*lmilimvwwllikmlkvvpiisminilgirmmqltfghlvqnirv
qmf*ltkpkgvqylheikdsmvsafqwisnegvlcgekmrsirfnvydvmlhadaihrgs
gqfipttrrvmyasqlsaqptllepmylvdistpesgisgiysvlnrrrgvvigeerrig
tplfnikaylpvmesfgltadlrsstagqafpqcvfdhwssigivgqxkk

Frame C:
hinnkyyyiyktkliklklyfspalakl*fafvqlfslnsyy*nsf*klsflkklkqkkx
gqfynrsnsfnygssxkyxkyvcyxscxsw*nysy*finskswyycx*giw*criyvmss
x*titxyyhkviicftsl*xak---

---YMKSEPIIVRPN**y**wfggf*src*kscqlsl**tflg*e*cs*hlviwsri*gs
kcfs*qnqrvfnifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cfmpmqfievv
vnlfqqlvv*cmppnyqlnqhfwnqciwlilvllkvvlvvstlysiveevlslvknvvlv
lhysisklifl*wnhlvslliyvqvllvklslnvfliigvqlvslvkxkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHA256 (SHA256Q) /CSM/SH/SHA2-C/SHA256Q.Seq.d/                       1742   0.0
VHJ795 (VHJ795Q) /CSM/VH/VHJ7-D/VHJ795Q.Seq.d/                       1711   0.0
VHB364 (VHB364Q) /CSM/VH/VHB3-C/VHB364Q.Seq.d/                       1338   0.0
AHM516 (AHM516Q) /CSM/AH/AHM5-A/AHM516Q.Seq.d/                       1338   0.0
AHB514 (AHB514Q) /CSM/AH/AHB5-A/AHB514Q.Seq.d/                       1338   0.0
AHD146 (AHD146Q) /CSM/AH/AHD1-B/AHD146Q.Seq.d/                       1336   0.0
AHB739 (AHB739Q) /CSM/AH/AHB7-B/AHB739Q.Seq.d/                       1336   0.0
VHP840 (VHP840Q) /CSM/VH/VHP8-B/VHP840Q.Seq.d/                       1322   0.0
AHL348 (AHL348Q) /CSM/AH/AHL3-B/AHL348Q.Seq.d/                       1322   0.0
AHC445 (AHC445Q) /CSM/AH/AHC4-B/AHC445Q.Seq.d/                       1322   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   141   1e-81  8
X15386|X15386.1 Dictyostelium discoideum H6 gene mRNA.            244   1e-70  3
BI863585|BI863585.1 kx46a06.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   4e-18  4
BI863575|BI863575.1 kx45h07.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CHICK Q90705 ELONGATION FACTOR 2 ;, mRNA sequence.    66   9e-15  3
BI451383|BI451383.1 kx23e09.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   9e-15  3
BM056853|BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA sequence.    92   3e-14  1
DR447725|DR447725.1 AR1047E10 A. gomesiana hemocytes normalized library Acanthoscurria gomesiana cDNA clone AR1047E10 5', mRNA sequence.    88   6e-14  2
DR443579|DR443579.1 AR2018A03 A. gomesiana hemocytes library Acanthoscurria gomesiana cDNA clone AR2018A03 5', mRNA sequence.    88   6e-14  2
BM058578|BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, mRNA sequence.    90   1e-13  1
BM058379|BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence.    90   1e-13  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   206   4e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   205   3e-65
BC063919_1(BC063919|pid:none) Xenopus tropicalis eukaryotic tran...   205   4e-65
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   204   4e-65
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   204   1e-64
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   204   1e-64
AK159514_1(AK159514|pid:none) Mus musculus osteoclast-like cell ...   204   1e-64
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   204   1e-64
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   204   1e-64
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   204   1e-64

 psg: 0.47  gvh: 0.43  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar

>> prediction for SHA256 is cyt

>SHA256F.Seq
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTGCATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAA
ANGGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCANA
AATATGTCTGTTATNGCTCATGTNGATCATGGTAAAACTACTCTTACTGATTCATTAATT
CAAAGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGT
CCANATGAACAATTACGNGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAA
ANGCCAAAAGNNNNNNNNNN

>SHA256Z.Seq
NNNNNNNNNNTCTACATGAAATCTGAACCCATTATCGTCAGACCTAACTGATGATATTGA
TAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAA
ACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGG
TCCAAATGTTTTAGTTAACAAAACCAAAGGGTGTTCAATATCTTCATGAAATTAAAGACT
CTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGA
GATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTA
GTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAAC
CAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTG
GTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTG
GTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTG
CTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGA
GTTCAATTGGTATCGTTGGTCAAGANAAAAAAA

>SHA256P.Seq
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTGCATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAA
ANGGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCANA
AATATGTCTGTTATNGCTCATGTNGATCATGGTAAAACTACTCTTACTGATTCATTAATT
CAAAGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGT
CCANATGAACAATTACGNGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAA
ANGCCAAAAG----------TCTACATGAAATCTGAACCCATTATCGTCAGACCTAACTG
ATGATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATC
TCTATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAG
AATATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGGTGTTCAATATCTTCATGAA
ATTAAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGT
GAGAAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATT
CATAGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTA
TCAGCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGT
GGTATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAA
CGTCGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTT
GGTCTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTT
GATCATTGGAGTTCAATTGGTATCGTTGGTCAAGANAAAAAAA