>SHA240 (SHA240Q) /CSM/SH/SHA2-B/SHA240Q.Seq.d/
TGGCCTACTGGAAAAAAAAAAGTTATTATATTATAATTTCATTATATATTTTTCATTTTA
TTAAAATTAATAATATATAATATATAATATATATATTACTTTTACTAATATATATTTTTT
TAATTTAAAAAAATGTTAATAAATAAAATTTTATTTTTATTTTTTATTCTATCACTAACA
ATATTTAATATTGTTGATGCTGGTTATACTTTATCTGTTAAAGGAAAAAAGTTAATATAT
ACTGGTGCCAATGTANATTTCAAAATTACTGAAATTTTGTTAACTTCCCAAAAAATATTA
CCAGATTACTCAATTACATTCACTTGTGAAATTCCATCTGCCAATAGCCCTGTTTGTACN
AGTATGGATATGGACTTTACAAAATTAGCTGGAAAATTAACTCTAAGGGGAGCAAATTTA
AAAACCCCAAATATAGTAATTGCCTATGATATTAAAGCCAATCAAATTCCACTACCATTA
CCAACTGAGGTTAAATGGGAACCACCAACAATGGCTGGTACAGCTGCTCTTATGACXXXX
XXXXXXTTGCCAAGTACATTATTGAGTGATTCATATGCCACCATTTCAGTTGATGGTATT
AAAAACTCAGACTCTGACCAACTTGATGTCGAGCTTCAACCAGTTGTCAAATCAATTACT
CAATCACAAACCTCTGGATCAACTGTGACAATTAAAGGTTCATTCTTTAATAAAAAGGAC
ACTGGTTTGAAAATTATGGTTGGAAATCATTCATGTGATTCTTTGACTATTGTAGATACC
CAAACACTTACTTGCTCTGCTCCACCAGGAAGTGGTATTAAAAATGCTGTTTCATTCATG
GTTAAAGATGCTGAATCAAAATTATCACCACCATTGTTTTTAGATTATAGTCCACCAACC
ATTATGTCATCAACAAGTGTAAAACAAGTTGAAGGTGGTCAAGTTACTATTTATGGTACC
AATTTTGAAAATACTTTGATTACAGTAAATGTTGGAAGTAAATCATGTGAAGATGTTTCT
GTAATAGATTCAACCAAACTTACTTGTTCAATTTCAAAATCAACAATAGATCCTTCATTA
ACAAATAAGAAATTGGCCGTAAATGTTACTGTAAATGGTCAATCTGCTAATGGTGAAGTA
TTTGAATATGATAATGAAGCACTCAATACTGAAACTCCTAAACCAACATTAGCACCAACT
ATAAAACCAACTGAAAAACCAACTGAAAAACCAACTGAAAAACCAACAACATCTCCAATA
GATAAACACATTGAAAATAGTTCTTCTAAAATTTCTAATTCATTAATTTTAATTTTCTCA
TTATATTACAATCCAAANATTTAAAA

wptgkkkviil*fhyiffillkliiyni*yiyyfy*yiff*FKKMLINKILFLFFILSLT
IFNIVDAGYTLSVKGKKLIYTGANVXFKITEILLTSQKILPDYSITFTCEIPSANSPVCT
SMDMDFTKLAGKLTLRGANLKTPNIVIAYDIKANQIPLPLPTEVKWEPPTMAGTAALM--
-

---LPSTLLSDSYATISVDGIKNSDSDQLDVELQPVVKSITQSQTSGSTVTIKGSFFNKK
DTGLKIMVGNHSCDSLTIVDTQTLTCSAPPGSGIKNAVSFMVKDAESKLSPPLFLDYSPP
TIMSSTSVKQVEGGQVTIYGTNFENTLITVNVGSKSCEDVSVIDSTKLTCSISKSTIDPS
LTNKKLAVNVTVNGQSANGEVFEYDNEALNTETPKPTLAPTIKPTEKPTEKPTEKPTTSP
IDKHIENSSSKISNSLILIFSLYYNPXI*

Frame A:
wptgkkkviil*fhyiffillkliiyni*yiyyfy*yiff*FKKMLINKILFLFFILSLT
IFNIVDAGYTLSVKGKKLIYTGANVXFKITEILLTSQKILPDYSITFTCEIPSANSPVCT
SMDMDFTKLAGKLTLRGANLKTPNIVIAYDIKANQIPLPLPTEVKWEPPTMAGTAALM--
-

---LPSTLLSDSYATISVDGIKNSDSDQLDVELQPVVKSITQSQTSGSTVTIKGSFFNKK
DTGLKIMVGNHSCDSLTIVDTQTLTCSAPPGSGIKNAVSFMVKDAESKLSPPLFLDYSPP
TIMSSTSVKQVEGGQVTIYGTNFENTLITVNVGSKSCEDVSVIDSTKLTCSISKSTIDPS
LTNKKLAVNVTVNGQSANGEVFEYDNEALNTETPKPTLAPTIKPTEKPTEKPTEKPTTSP
IDKHIENSSSKISNSLILIFSLYYNPXI*

Frame B:
gllekkkllyynfiiyfsfy*n**yiiyniyitftniyffnlkkc**ikfyfyflfyh*q
ylillmlvilyllkeks*yilvpmxiskllkfc*lpkkyyqitqlhslvkfhlpialfvx
vwiwtlqn*len*l*geqi*kpqi**lpmilkpikfhyhyqlrlngnhqqwlvqlll*--
-

---cqvhy*vihmppfqlmvlktqtltnlmssfnqlsnqllnhkpldql*qlkvhslikr
tlv*klwleiihvil*ll*ipkhllallhqevvlkmlfhswlkmlnqnyhhhcf*iivhq
plchqqv*nklkvvkllfmvpilkil*lq*mlevnhvkmfl**iqpnllvqfqnqq*ilh
*qirnwp*mll*mvnllmvkylnmimkhsilkllnqh*hql*nqlknqlknqlknqqhlq
*intlkivllkflih*f*fshyitiqxfk

Frame C:
aywkkksyyiiislyifhfikinni*yiiyilllliyifli*knvnk*nfififysitnn
i*yc*cwlyfic*rkkvniywcqcxfqny*nfvnfpknitrllnyihl*nsicq*pclyx
ygyglykiswkinskgskfknpkysncl*y*sqsnsttitn*g*mgttnngwyscsyd--
-

---akyiie*fichhfs*wy*klrl*pt*crastscqinysitnlwincdn*rfil**kg
hwfenygwksfm*ffdycrypntyllcstrkwy*kccfihg*rc*ikiittivfrl*stn
hyvinkckts*rwssyylwyqf*kyfdyskcwk*im*rcfcnrfnqtylfnfkinnrsfi
nk*eigrkcyckwsic*w*si*i***stqy*ns*tnistnyktn*ktn*ktn*ktnnisn
r*th*k*ff*nf*finfnfliilqskxlk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHA240 (SHA240Q) /CSM/SH/SHA2-B/SHA240Q.Seq.d/                       1965   0.0
SHL869 (SHL869Q) /CSM/SH/SHL8-C/SHL869Q.Seq.d/                       1281   0.0
SHK779 (SHK779Q) /CSM/SH/SHK7-D/SHK779Q.Seq.d/                       1281   0.0
SHG859 (SHG859Q) /CSM/SH/SHG8-C/SHG859Q.Seq.d/                       1281   0.0
SHG451 (SHG451Q) /CSM/SH/SHG4-C/SHG451Q.Seq.d/                       1281   0.0
SHD289 (SHD289Q) /CSM/SH/SHD2-D/SHD289Q.Seq.d/                       1281   0.0
SHB402 (SHB402Q) /CSM/SH/SHB4-A/SHB402Q.Seq.d/                       1281   0.0
SHA422 (SHA422Q) /CSM/SH/SHA4-A/SHA422Q.Seq.d/                       1281   0.0
SHA372 (SHA372Q) /CSM/SH/SHA3-C/SHA372Q.Seq.d/                       1281   0.0
SHA315 (SHA315Q) /CSM/SH/SHA3-A/SHA315Q.Seq.d/                       1281   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    38   0.15   11
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    32   1.7    15
CZ518228|CZ518228.1 GMW2-73G17a.b1 GMW2 Glycine max genomic, genomic survey sequence.    46   1.9    1
AW375848|AW375848.1 RC3-CT0197-220999-022-A10 CT0197 Homo sapiens cDNA, mRNA sequence.    40   4.0    2
BX248396|BX248396.10 Zebrafish DNA sequence from clone CH211-219M13.    34   4.4    8
AL103054|AL103054.1 Drosophila melanogaster genome survey sequence T7 end of BAC  BACN10O21 of DrosBAC library from Drosophila melanogaster (fruit  fly).    32   4.8    2
BF297343|BF297343.1 050PbE09 Pb cDNA #17, Tommaso Pace, Marta Ponzi, and Clara Frontali Plasmodium berghei cDNA 5', mRNA sequence.    44   7.6    1
CX078364|CX078364.1 UCRCS08_8F05_b Parent Washington Navel Orange Callus cDNA Library UCRCS08-2 Citrus sinensis cDNA clone UCRCS08-8F05-L10-1-5.b, mRNA sequence.    44   7.6    1
CK295567|CK295567.1 EST758281 Nicotiana benthamiana mixed tissue cDNA library, normalized, full-length Nicotiana benthamiana cDNA clone NBMCV80 5' end, mRNA sequence.    44   7.6    1
AA535387|AA535387.1 nf94h11.s1 NCI_CGAP_Co3 Homo sapiens cDNA clone IMAGE:927621 3', mRNA sequence.    44   7.6    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117076_23(AC117076|pid:none) Dictyostelium discoideum chromoso...    91   9e-17
AC115598_15(AC115598|pid:none) Dictyostelium discoideum chromoso...    71   1e-10
AC116100_1(AC116100|pid:none) Dictyostelium discoideum chromosom...    69   3e-10
(Q06885) RecName: Full=Glycoprotein gp100; AltName: Full=P29F8; ...    62   3e-08
FJ374909_1(FJ374909|pid:none) Dictyostelium discoideum strain QS...    62   4e-08
AC116963_25(AC116963|pid:none) Dictyostelium discoideum chromoso...    60   1e-07
FJ374921_1(FJ374921|pid:none) Dictyostelium discoideum strain QS...    60   2e-07
FJ374902_1(FJ374902|pid:none) Dictyostelium discoideum strain QS...    59   3e-07
FJ374971_1(FJ374971|pid:none) Dictyostelium discoideum strain AX...    59   5e-07
FJ374905_1(FJ374905|pid:none) Dictyostelium discoideum strain QS...    58   8e-07

 psg: 1.02  gvh: 0.61  alm: 0.23  top: 0.40  tms: 0.07  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SHA240 is end

>SHA240F.Seq
TGGCCTACTGGAAAAAAAAAAGTTATTATATTATAATTTCATTATATATTTTTCATTTTA
TTAAAATTAATAATATATAATATATAATATATATATTACTTTTACTAATATATATTTTTT
TAATTTAAAAAAATGTTAATAAATAAAATTTTATTTTTATTTTTTATTCTATCACTAACA
ATATTTAATATTGTTGATGCTGGTTATACTTTATCTGTTAAAGGAAAAAAGTTAATATAT
ACTGGTGCCAATGTANATTTCAAAATTACTGAAATTTTGTTAACTTCCCAAAAAATATTA
CCAGATTACTCAATTACATTCACTTGTGAAATTCCATCTGCCAATAGCCCTGTTTGTACN
AGTATGGATATGGACTTTACAAAATTAGCTGGAAAATTAACTCTAAGGGGAGCAAATTTA
AAAACCCCAAATATAGTAATTGCCTATGATATTAAAGCCAATCAAATTCCACTACCATTA
CCAACTGAGGTTAAATGGGAACCACCAACAATGGCTGGTACAGCTGCTCTTATGACNNNN
NNNNNN

>SHA240Z.Seq
NNNNNNNNNNTTGCCAAGTACATTATTGAGTGATTCATATGCCACCATTTCAGTTGATGG
TATTAAAAACTCAGACTCTGACCAACTTGATGTCGAGCTTCAACCAGTTGTCAAATCAAT
TACTCAATCACAAACCTCTGGATCAACTGTGACAATTAAAGGTTCATTCTTTAATAAAAA
GGACACTGGTTTGAAAATTATGGTTGGAAATCATTCATGTGATTCTTTGACTATTGTAGA
TACCCAAACACTTACTTGCTCTGCTCCACCAGGAAGTGGTATTAAAAATGCTGTTTCATT
CATGGTTAAAGATGCTGAATCAAAATTATCACCACCATTGTTTTTAGATTATAGTCCACC
AACCATTATGTCATCAACAAGTGTAAAACAAGTTGAAGGTGGTCAAGTTACTATTTATGG
TACCAATTTTGAAAATACTTTGATTACAGTAAATGTTGGAAGTAAATCATGTGAAGATGT
TTCTGTAATAGATTCAACCAAACTTACTTGTTCAATTTCAAAATCAACAATAGATCCTTC
ATTAACAAATAAGAAATTGGCCGTAAATGTTACTGTAAATGGTCAATCTGCTAATGGTGA
AGTATTTGAATATGATAATGAAGCACTCAATACTGAAACTCCTAAACCAACATTAGCACC
AACTATAAAACCAACTGAAAAACCAACTGAAAAACCAACTGAAAAACCAACAACATCTCC
AATAGATAAACACATTGAAAATAGTTCTTCTAAAATTTCTAATTCATTAATTTTAATTTT
CTCATTATATTACAATCCAAANATTTAAAA

>SHA240P.Seq
TGGCCTACTGGAAAAAAAAAAGTTATTATATTATAATTTCATTATATATTTTTCATTTTA
TTAAAATTAATAATATATAATATATAATATATATATTACTTTTACTAATATATATTTTTT
TAATTTAAAAAAATGTTAATAAATAAAATTTTATTTTTATTTTTTATTCTATCACTAACA
ATATTTAATATTGTTGATGCTGGTTATACTTTATCTGTTAAAGGAAAAAAGTTAATATAT
ACTGGTGCCAATGTANATTTCAAAATTACTGAAATTTTGTTAACTTCCCAAAAAATATTA
CCAGATTACTCAATTACATTCACTTGTGAAATTCCATCTGCCAATAGCCCTGTTTGTACN
AGTATGGATATGGACTTTACAAAATTAGCTGGAAAATTAACTCTAAGGGGAGCAAATTTA
AAAACCCCAAATATAGTAATTGCCTATGATATTAAAGCCAATCAAATTCCACTACCATTA
CCAACTGAGGTTAAATGGGAACCACCAACAATGGCTGGTACAGCTGCTCTTATGAC----
------TTGCCAAGTACATTATTGAGTGATTCATATGCCACCATTTCAGTTGATGGTATT
AAAAACTCAGACTCTGACCAACTTGATGTCGAGCTTCAACCAGTTGTCAAATCAATTACT
CAATCACAAACCTCTGGATCAACTGTGACAATTAAAGGTTCATTCTTTAATAAAAAGGAC
ACTGGTTTGAAAATTATGGTTGGAAATCATTCATGTGATTCTTTGACTATTGTAGATACC
CAAACACTTACTTGCTCTGCTCCACCAGGAAGTGGTATTAAAAATGCTGTTTCATTCATG
GTTAAAGATGCTGAATCAAAATTATCACCACCATTGTTTTTAGATTATAGTCCACCAACC
ATTATGTCATCAACAAGTGTAAAACAAGTTGAAGGTGGTCAAGTTACTATTTATGGTACC
AATTTTGAAAATACTTTGATTACAGTAAATGTTGGAAGTAAATCATGTGAAGATGTTTCT
GTAATAGATTCAACCAAACTTACTTGTTCAATTTCAAAATCAACAATAGATCCTTCATTA
ACAAATAAGAAATTGGCCGTAAATGTTACTGTAAATGGTCAATCTGCTAATGGTGAAGTA
TTTGAATATGATAATGAAGCACTCAATACTGAAACTCCTAAACCAACATTAGCACCAACT
ATAAAACCAACTGAAAAACCAACTGAAAAACCAACTGAAAAACCAACAACATCTCCAATA
GATAAACACATTGAAAATAGTTCTTCTAAAATTTCTAATTCATTAATTTTAATTTTCTCA
TTATATTACAATCCAAANATTTAAAA