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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI692
Library AF
(Link to library)
Clone ID AFI692
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFI692P
(Link to Original site)
Representative DNA sequence 
>AFI692 (AFI692Q) /CSM/AF/AFI6-D/AFI692Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACXXXXXXXXXXCGTAAA
GGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAAT
CGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGA
AGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAA
TCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAATACGCTGAAGA
CAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAA
ATCTGGTTCCATCTTTAACAATACGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTT
CTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGA
AGCCGCCAAAGCTGAANANAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGC
TGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGT
TAAAACTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQY---

---VKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKA
KKIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNTIVXDSVE
EAKDFXEKTFVANQEAEKKMFDDLEAAKAEXRKKADEKLAAEKAAEKEAEEADEEEEEVA
EEDLVKTDXKKEEVKKSTKKV
Translated Amino Acid sequence (All Frames) 
Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQY---

---rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*mes
kenpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrxrlcr
rs*rfl*kdlccqprsrkenv*rprsrqs*xxkes*rkiscrksc*krsrrs**rrrrsr
*rrfs*n*xqkrrsqkinqks

Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lint---

---VKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKA
KKIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNTIVXDSVE
EAKDFXEKTFVANQEAEKKMFDDLEAAKAEXRKKADEKLAAEKAAEKEAEEADEEEEEVA
EEDLVKTDXKKEEVKKSTKKV

Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssih---

---*rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkq
rksqiqntkvngxth*litqntlktmnstslmisvlsvsnygklnlvpsltirssptlsk
klkisxkrpllptkkpkrkclttskppklxxerkltkn*lpkkllkkkpkklmkkkkksl
kki*lklmxkkkksknqpkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        932   0.0
VFM885 (VFM885Q) /CSM/VF/VFM8-D/VFM885Q.Seq.d/                        932   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        932   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        932   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        932   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        932   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        932   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        932   0.0
VFH220 (VFH220Q) /CSM/VF/VFH2-A/VFH220Q.Seq.d/                        932   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                        932   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   932   0.0    4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   2e-23  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    74   2e-15  3
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
BT013428|BT013428.1 Lycopersicon esculentum clone 132068F, mRNA sequence.    74   4e-10  2
CK247437|CK247437.1 EST731074 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCAT37 5' end, mRNA sequence.    66   4e-09  3
CK253744|CK253744.1 EST737381 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCC781 5' end, mRNA sequence.    66   4e-09  3
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   6e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   6e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   6e-09  1
dna update 2004. 9.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   329   1e-88
BT072764_1(BT072764|pid:none) Salmo salar clone ssal-rgf-539-230...   185   6e-81
L24159_1(L24159|pid:none) Schistosoma mansoni calreticulin gene,...   147   1e-65
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   208   3e-52
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   207   6e-52
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   207   6e-52
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   205   2e-51
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   205   3e-51
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   205   3e-51
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   204   5e-51
protein update 2009. 4.23
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for AFI692 is exc
5' end seq. ID AFI692F
5' end seq. 
>AFI692F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACAC----------
Length of 5' end seq. 584
3' end seq. ID AFI692Z
3' end seq. 
>AFI692Z.Seq
----------CGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACG
ATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAG
ACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAG
CAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACC
CAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCG
AATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATACGATCGTCNCCGACTCTGTCG
AAGAAGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAA
TGTTTGACGACCTCGAAGCCGCCAAAGCTGAANANAGAAAGAAAGCTGACGAAAAATTAG
CTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCG
CTGAAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAA
AAGTT
Length of 3' end seq. 595
Connected seq. ID AFI692P
Connected seq. 
>AFI692P.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACAC----------CGTAAA
GGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAAT
CGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGA
AGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAA
TCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAATACGCTGAAGA
CAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAA
ATCTGGTTCCATCTTTAACAATACGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTT
CTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGA
AGCCGCCAAAGCTGAANANAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGC
TGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGT
TAAAACTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTT
Length of connected seq. 1179
Full length Seq ID -
Full length Seq. -
Length of full length seq. -