>AFA889 (AFA889Q) /CSM/AF/AFA8-D/AFA889Q.Seq.d/
XXXXXXXXXXTTTTCAATGGCGACTTTGTGGATAGGGGTAGTTTTAGTTTTGAAATTATC
ATCACTCTATTGGCTTATAAATTATTGTACCCAAATCATATGCATTTGACAAGGGGTAAC
CATGAGACAACCGATATGAATCGTTTCTATGGTTTCCAAGGCGAAGTGGTTGCAAAATAC
AGTGAGATGGTATTCGATCTATTCTCAGAGTTGTTCAATTGGTTCCCATTGGCATTCGTG
TTGGATGAATCGTTTATGGTGGTACATGGTGGTTTGTTTGGTAAGGATGGTGTCACACTT
GACGATATTAGAAAGATTAAACGTAATGCACCAGATCCAAAAGATAATAAATTGGTTCAA
TGCTTACTTTGGTCTGATCCTCAATCAAATCCTGGTATTGCTCCTTCTTCAAGAGGTGTA
GGTGTTTACTTTGGACCTGATGTCACCAGACGTTTCCTCAAGGAAAATAATCTATCAACT
ATCATTAGATCTCATGAAGTTAAAGAAAAAGGTTATCAAATCGATGATGATGGTTCTCTC
ATCACTGTTTTCTCTGCTCCAAATTATTGTGATCAATCTGGTAATCTTGGCTCATTTATA
AATATAACTGAAGATAAAATTAAAATTACAACTTTTGAAGCTGTGAACATCCGAATATAC
CACCAA

---FNGDFVDRGSFSFEIIITLLAYKLLYPNHMHLTRGNHETTDMNRFYGFQGEVVAKYS
EMVFDLFSELFNWFPLAFVLDESFMVVHGGLFGKDGVTLDDIRKIKRNAPDPKDNKLVQC
LLWSDPQSNPGIAPSSRGVGVYFGPDVTRRFLKENNLSTIIRSHEVKEKGYQIDDDGSLI
TVFSAPNYCDQSGNLGSFINITEDKIKITTFEAVNIRIYHQ

Frame A:
---fqwrlcg*g*f*f*nyhhsigl*iivpksyafdkg*p*dnryesflwfprrsgckiq
*dgirsilrvvqlvpigirvg*ivyggtwwfvw*gwcht*ry*kd*t*ctrskr**igsm
ltlv*ssikswycsffkrcrcllwt*chqtfpqgk*sinyh*is*s*rkrlsnr**wfsh
hcflcskll*siw*swliykyn*r*n*nynf*scehpnipp


Frame B:
---FNGDFVDRGSFSFEIIITLLAYKLLYPNHMHLTRGNHETTDMNRFYGFQGEVVAKYS
EMVFDLFSELFNWFPLAFVLDESFMVVHGGLFGKDGVTLDDIRKIKRNAPDPKDNKLVQC
LLWSDPQSNPGIAPSSRGVGVYFGPDVTRRFLKENNLSTIIRSHEVKEKGYQIDDDGSLI
TVFSAPNYCDQSGNLGSFINITEDKIKITTFEAVNIRIYHQ


Frame C:
---smatlwigvvlvlklsslywlinyctqiici*qgvtmrqpi*ivsmvskakwlqntv
rwysiysqscsigshwhscwmnrlwwymvvclvrmvshltilerlnvmhqiqkiinwfna
yfglilnqilvllllqev*vftldlmspdvssrkiiyqlsldlmklkkkviksmmmvlss
lfsllqiivinlvilahl*i*lkiklklqllkl*tseytt

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA889 (AFA889Q) /CSM/AF/AFA8-D/AFA889Q.Seq.d/                       1300   0.0
SSE754 (SSE754Q) /CSM/SS/SSE7-C/SSE754Q.Seq.d/                       1279   0.0
VFI734 (VFI734Q) /CSM/VF/VFI7-B/VFI734Q.Seq.d/                       1277   0.0
AFO461 (AFO461Q) /CSM/AF/AFO4-C/AFO461Q.Seq.d/                       1277   0.0
SFG624 (SFG624Q) /CSM/SF/SFG6-A/SFG624Q.Seq.d/                       1271   0.0
AFL662 (AFL662Q) /CSM/AF/AFL6-C/AFL662Q.Seq.d/                       1271   0.0
VFF491 (VFF491Q) /CSM/VF/VFF4-D/VFF491Q.Seq.d/                       1269   0.0
CFC823 (CFC823Q) /CSM/CF/CFC8-A/CFC823Q.Seq.d/                       1267   0.0
SFD165 (SFD165Q) /CSM/SF/SFD1-C/SFD165Q.Seq.d/                       1263   0.0
CFG219 (CFG219Q) /CSM/CF/CFG2-A/CFG219Q.Seq.d/                       1227   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

CA933944|CA933944.1 MTU3TS.P14.F11 Aspen stem cDNA Library Populus tremuloides cDNA, mRNA sequence.    54  3e-05  2
BU041737|BU041737.1 PP_LEa0010F03f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0010F03f, mRNA sequence.    60  4e-05  1
CB821514|CB821514.1 EST 2365 Half-Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone be002m12 3', mRNA sequence.    60  4e-05  1
BF272976|BF272976.1 GA__Eb0016H03f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Eb0016H03f, mRNA sequence.    48  4e-05  2
BQ916199|BQ916199.1 QHB17B15.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB17B15, mRNA sequence.    58  1e-04  1
AJ561748|AJ561748.1 Cryptosporidium parvum GSS, PAC clone pica_0004_g07, T7 end  sequence.    34  7e-04  3
BU822525|BU822525.1 UB38BPH04 Populus tremula cambium cDNA library Populus tremula cDNA 5 prime, mRNA sequence.    54  0.002  1
BU895987|BU895987.1 X033H08 Populus wood cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    54  0.002  1
AV202542|AV202542.1 Caenorhabditis elegans cDNA clone:yk551g8 : 5' end, single read.    44  0.005  2
C68648|C68648.1 C.elegans cDNA clone yk306a6 : 5' end, single read.    44  0.005  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR848198_1(CR848198|pid:none) Xenopus tropicalis finished cDNA, ...   257   2e-67
BC080162_1(BC080162|pid:none) Xenopus tropicalis ppp5c protein, ...   257   2e-67
CR859667_1(CR859667|pid:none) Pongo abelii mRNA; cDNA DKFZp459E1...   255   9e-67
(P53041) RecName: Full=Serine/threonine-protein phosphatase 5;  ...   254   1e-66
AK297095_1(AK297095|pid:none) Homo sapiens cDNA FLJ55717 complet...   254   1e-66
AK223222_1(AK223222|pid:none) Homo sapiens mRNA for protein phos...   254   1e-66
BC000750_1(BC000750|pid:none) Homo sapiens protein phosphatase 5...   254   1e-66
AK293402_1(AK293402|pid:none) Homo sapiens cDNA FLJ55048 complet...   254   1e-66
(Q84K11) RecName: Full=Serine/threonine protein phosphatase 5;  ...   254   2e-66
BC078786_1(BC078786|pid:none) Rattus norvegicus protein phosphat...   253   4e-66

 psg: 0.75  gvh: 0.37  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFA889 is cyt

>AFA889Z.Seq
----------TTTTCAATGGCGACTTTGTGGATAGGGGTAGTTTTAGTTTTGAAATTATC
ATCACTCTATTGGCTTATAAATTATTGTACCCAAATCATATGCATTTGACAAGGGGTAAC
CATGAGACAACCGATATGAATCGTTTCTATGGTTTCCAAGGCGAAGTGGTTGCAAAATAC
AGTGAGATGGTATTCGATCTATTCTCAGAGTTGTTCAATTGGTTCCCATTGGCATTCGTG
TTGGATGAATCGTTTATGGTGGTACATGGTGGTTTGTTTGGTAAGGATGGTGTCACACTT
GACGATATTAGAAAGATTAAACGTAATGCACCAGATCCAAAAGATAATAAATTGGTTCAA
TGCTTACTTTGGTCTGATCCTCAATCAAATCCTGGTATTGCTCCTTCTTCAAGAGGTGTA
GGTGTTTACTTTGGACCTGATGTCACCAGACGTTTCCTCAAGGAAAATAATCTATCAACT
ATCATTAGATCTCATGAAGTTAAAGAAAAAGGTTATCAAATCGATGATGATGGTTCTCTC
ATCACTGTTTTCTCTGCTCCAAATTATTGTGATCAATCTGGTAATCTTGGCTCATTTATA
AATATAACTGAAGATAAAATTAAAATTACAACTTTTGAAGCTGTGAACATCCGAATATAC
CACCAA