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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHA130
Library SH
(Link to library)
Clone ID SHA130
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11515-1
Original site URL
Representative seq. ID SHA130P
(Link to Original site)
Representative DNA sequence 
>SHA130 (SHA130Q) /CSM/SH/SHA1-B/SHA130Q.Seq.d/
CAATATAATACAACTAATACAACTACACCTAACACAACCCAAAATTATGATTATAGTAAT
ACTAGTGGTAATAGAAATAGTGGTCAATATGATCAATATACTACAACTAATACAACCAGT
GCTAATACTTATGGTTATGATAATTCTAAAACACATTCACCAACACCATCAAATACTCCA
TCTGCAACTATTAATTCTTGGGATTATAATCAACAACAACCACAACCACAACAACCACAA
TCAGTATATAATAATCCATCATCATCAACCACAAATACAACTTACAATAATCCATCAACA
ACAACAACAACATCATCACCAACAAATACAAACACAACTTATAATAATCCATCATCAGCC
ACAAATACAAATTATAATAATAGAAATTCATGGGGATATGATCAATCAACTTATAATAAC
TCATCATATAATAATAATACTGATACATATTCAAATCAAACTCAACAACAAGAATATCAA
GTACTTTATGATAAAAAATATCAAGAAGAAGAAGAAAAGAGAAGAAAAGAATATGAAGAA
TCATGGAGAGTTTATTATGAACAACAAGCAGCTTATGAGAAAGAACAAGAAAGAATTAGA
AATGAAGAGATGGAAAXXXXXXXXXXAAGTTGAAGCGAGGGTTACAAAATTATTTAAAAG
TGAAGAAATTAATAAGGTATTTTCAAATTTAAAAAGTATCCTTCAAATTAGTACAGAGAT
TACTCATCTTTTAGAAGAAAGATTATCAGTTTCACCTGTTAAAATTGGTGATATTTTTGT
TAAATTTGCACCAATTTTTAAAATTTATGTGGAATATGTAAATAATTTTGATAAAGTAGC
ACCACAATTAAAAGCAATGACAGAGAATCCACAATCAAAGAAATTCTTTTCAGAACAAAG
AAATAAATCAAGAGTAGCAACTGATATTAATAGTTTATTAATTATGCCAGTTCAACGTTG
TCCAAGATATGAATTATTATTAAGGGAAATTTTAAAACACACTCAAGAGGATCATGTTGA
ATTTAAAAATATTAAAAGTGCTTATGAATCAATCAAAGATATTAATAAGTATATTAATGA
TAGAAAGAGAAATGTTGATAATAGAGCAAAATTATTAGAGTTACAAAAAGAAATTAAAAA
TGCACCAGAATTAATTGAATCTCATAGATATTTTGTTAGAGAGTCACAATGTAATATCTC
TGCAAATAAGAAAAGTGAAAGTGGTGGTTTTTATTTATTCTTTTTCAATGATATGGTGTT
AGTCACAAAGAAATCTAGCCTTTTTTCAAATTATAAATACGTTTACACAATTCCTTGAAA
AATGCAGACATTAAAGATATTAGTTCAAANATTCAATGATACGTATTATTGTGGTTCTCT
GAATCAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
QYNTTNTTTPNTTQNYDYSNTSGNRNSGQYDQYTTTNTTSANTYGYDNSKTHSPTPSNTP
SATINSWDYNQQQPQPQQPQSVYNNPSSSTTNTTYNNPSTTTTTSSPTNTNTTYNNPSSA
TNTNYNNRNSWGYDQSTYNNSSYNNNTDTYSNQTQQQEYQVLYDKKYQEEEEKRRKEYEE
SWRVYYEQQAAYEKEQERIRNEEME---

---VEARVTKLFKSEEINKVFSNLKSILQISTEITHLLEERLSVSPVKIGDIFVKFAPIF
KIYVEYVNNFDKVAPQLKAMTENPQSKKFFSEQRNKSRVATDINSLLIMPVQRCPRYELL
LREILKHTQEDHVEFKNIKSAYESIKDINKYINDRKRNVDNRAKLLELQKEIKNAPELIE
SHRYFVRESQCNISANKKSESGGFYLFFFNDMVLVTKKSSLFSNYKYVYTIP*kmqtlki
lvqxfndtyycgslnq
Translated Amino Acid sequence (All Frames) 
Frame A:
QYNTTNTTTPNTTQNYDYSNTSGNRNSGQYDQYTTTNTTSANTYGYDNSKTHSPTPSNTP
SATINSWDYNQQQPQPQQPQSVYNNPSSSTTNTTYNNPSTTTTTSSPTNTNTTYNNPSSA
TNTNYNNRNSWGYDQSTYNNSSYNNNTDTYSNQTQQQEYQVLYDKKYQEEEEKRRKEYEE
SWRVYYEQQAAYEKEQERIRNEEME---

---klkrglqnylkvkkliryfqi*kvsfklvqrllif*kkdyqfhllklvifllnlhqf
lkfmwnm*iilik*hhn*kq*qrihnqrnsfqnkeinqe*qlilivy*lcqfnvvqdmny
y*gkf*ntlkrimlnlkilkvlmnqskilisilmieremliieqny*sykkklkmhqn*l
nlidilleshnvislqirkvkvvvfiysfsmiwc*sqrnlaffqiintftqflekcrh*r
y*fkxsmiriivvl*in

Frame B:
niiqliqlhltqpkimiivilvvieivvnminilqliqpvlilmvmiilkhihqhhqilh
lqllilgiiinnnhnhnnhnqyiiihhhqpqiqltiihqqqqqhhhqqiqtqliiihhqp
qiqiiiieihgdminqliithhiiiilihiqiklnnknikyfmiknikkkkkreeknmkn
hgefimnnkqlmrknkkelemkrwk---

---s*segykii*k*rn**gifkfkkypsn*yrdyssfrrkiisftc*nw*yfc*ictnf
*nlcgick*f**ssttiksndrestikeilfrtkk*ikssn*y**finyasstlski*ii
ikgnfkthsrgsc*i*ky*kcl*inqry**vy***kekc***skiirvtkrn*kctrin*
is*ifc*rvtm*ylck*ek*kwwflfilfq*ygvshkei*pffkl*irlhnslknadikd
issxiq*yvllwfses

Frame C:
i*yn*ynyt*hnpkl*l**y*w**k*wsi*siyyn*ynqc*ylwl**f*ntftntikysi
cny*flgl*stttttttttisi**siiinhkynlq*sinnnnniitnkykhnl**siish
kykl***kfmgi*sinl**lii***y*yifksnsttrisstl**kisrrrrkekkri*ri
mesll*ttssl*ertrkn*k*rdg---

---VEARVTKLFKSEEINKVFSNLKSILQISTEITHLLEERLSVSPVKIGDIFVKFAPIF
KIYVEYVNNFDKVAPQLKAMTENPQSKKFFSEQRNKSRVATDINSLLIMPVQRCPRYELL
LREILKHTQEDHVEFKNIKSAYESIKDINKYINDRKRNVDNRAKLLELQKEIKNAPELIE
SHRYFVRESQCNISANKKSESGGFYLFFFNDMVLVTKKSSLFSNYKYVYTIP*kmqtlki
lvqxfndtyycgslnq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHA130 (SHA130Q) /CSM/SH/SHA1-B/SHA130Q.Seq.d/                       1616   0.0
SHL631 (SHL631Q) /CSM/SH/SHL6-B/SHL631Q.Seq.d/                       1431   0.0
SSF856 (SSF856Q) /CSM/SS/SSF8-C/SSF856Q.Seq.d/                       1128   0.0
SHK851 (SHK851Q) /CSM/SH/SHK8-C/SHK851Q.Seq.d/                       1090   0.0
SHB511 (SHB511Q) /CSM/SH/SHB5-A/SHB511Q.Seq.d/                       1025   0.0
SHC432 (SHC432Q) /CSM/SH/SHC4-B/SHC432Q.Seq.d/                        997   0.0
SFJ674 (SFJ674Q) /CSM/SF/SFJ6-D/SFJ674Q.Seq.d/                        995   0.0
SHL216 (SHL216Q) /CSM/SH/SHL2-A/SHL216Q.Seq.d/                        948   0.0
AFH770 (AFH770Q) /CSM/AF/AFH7-C/AFH770Q.Seq.d/                        779   0.0
AHP429 (AHP429Q) /CSM/AH/AHP4-B/AHP429Q.Seq.d/                        745   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.   716   0.0    10
AC005308|AC005308.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces.    34   1e-05  13
AC005139|AC005139.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 5 unordered pieces.    36   2e-04  16
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    40   2e-04  17
BA000026|BA000026.2 Mycoplasma penetrans HF-2 DNA, complete genome.    34   3e-04  24
BH134015|BH134015.1 ENTOF91TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   5e-04  2
AZ678024|AZ678024.1 ENTGA39TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   5e-04  2
AZ531395|AZ531395.1 ENTBX39TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   5e-04  2
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           38   7e-04  17
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    36   7e-04  17
dna update 2005.12. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXH8) RecName: Full=Guanine exchange factor for Rac 30;            113   2e-23
T18289(T18289) racGAP protein - slime mold (Dictyostelium discoi...   113   2e-23
AK149626_1(AK149626|pid:none) Mus musculus bone marrow macrophag...   110   1e-22
(Q91ZT5) RecName: Full=FYVE, RhoGEF and PH domain-containing pro...   110   1e-22
AF402613_1(AF402613|pid:none) Mus musculus actin-binding protein...   110   1e-22
AB173388_1(AB173388|pid:none) Macaca fascicularis brain cDNA clo...   109   3e-22
(Q96M96) RecName: Full=FYVE, RhoGEF and PH domain-containing pro...   109   3e-22
AK057294_1(AK057294|pid:none) Homo sapiens cDNA FLJ32732 fis, cl...   109   3e-22
AK303334_1(AK303334|pid:none) Homo sapiens cDNA FLJ56188 complet...   106   2e-21
BC045552_1(BC045552|pid:none) Homo sapiens FYVE, RhoGEF and PH d...   105   3e-21
protein update 2009. 4.14
PSORT 

 psg: 0.72  gvh: 0.26  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.21
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 32.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for SHA130 is cyt
5' end seq. ID SHA130F
5' end seq. 
>SHA130F.Seq
CAATATAATACAACTAATACAACTACACCTAACACAACCCAAAATTATGATTATAGTAAT
ACTAGTGGTAATAGAAATAGTGGTCAATATGATCAATATACTACAACTAATACAACCAGT
GCTAATACTTATGGTTATGATAATTCTAAAACACATTCACCAACACCATCAAATACTCCA
TCTGCAACTATTAATTCTTGGGATTATAATCAACAACAACCACAACCACAACAACCACAA
TCAGTATATAATAATCCATCATCATCAACCACAAATACAACTTACAATAATCCATCAACA
ACAACAACAACATCATCACCAACAAATACAAACACAACTTATAATAATCCATCATCAGCC
ACAAATACAAATTATAATAATAGAAATTCATGGGGATATGATCAATCAACTTATAATAAC
TCATCATATAATAATAATACTGATACATATTCAAATCAAACTCAACAACAAGAATATCAA
GTACTTTATGATAAAAAATATCAAGAAGAAGAAGAAAAGAGAAGAAAAGAATATGAAGAA
TCATGGAGAGTTTATTATGAACAACAAGCAGCTTATGAGAAAGAACAAGAAAGAATTAGA
AATGAAGAGATGGAAANNNNNNNNNN
Length of 5' end seq. 626
3' end seq. ID SHA130Z
3' end seq. 
>SHA130Z.Seq
NNNNNNNNNNAAGTTGAAGCGAGGGTTACAAAATTATTTAAAAGTGAAGAAATTAATAAG
GTATTTTCAAATTTAAAAAGTATCCTTCAAATTAGTACAGAGATTACTCATCTTTTAGAA
GAAAGATTATCAGTTTCACCTGTTAAAATTGGTGATATTTTTGTTAAATTTGCACCAATT
TTTAAAATTTATGTGGAATATGTAAATAATTTTGATAAAGTAGCACCACAATTAAAAGCA
ATGACAGAGAATCCACAATCAAAGAAATTCTTTTCAGAACAAAGAAATAAATCAAGAGTA
GCAACTGATATTAATAGTTTATTAATTATGCCAGTTCAACGTTGTCCAAGATATGAATTA
TTATTAAGGGAAATTTTAAAACACACTCAAGAGGATCATGTTGAATTTAAAAATATTAAA
AGTGCTTATGAATCAATCAAAGATATTAATAAGTATATTAATGATAGAAAGAGAAATGTT
GATAATAGAGCAAAATTATTAGAGTTACAAAAAGAAATTAAAAATGCACCAGAATTAATT
GAATCTCATAGATATTTTGTTAGAGAGTCACAATGTAATATCTCTGCAAATAAGAAAAGT
GAAAGTGGTGGTTTTTATTTATTCTTTTTCAATGATATGGTGTTAGTCACAAAGAAATCT
AGCCTTTTTTCAAATTATAAATACGTTTACACAATTCCTTGAAAAATGCAGACATTAAAG
ATATTAGTTCAAANATTCAATGATACGTATTATTGTGGTTCTCTGAATCAAT
Length of 3' end seq. 772
Connected seq. ID SHA130P
Connected seq. 
>SHA130P.Seq
CAATATAATACAACTAATACAACTACACCTAACACAACCCAAAATTATGATTATAGTAAT
ACTAGTGGTAATAGAAATAGTGGTCAATATGATCAATATACTACAACTAATACAACCAGT
GCTAATACTTATGGTTATGATAATTCTAAAACACATTCACCAACACCATCAAATACTCCA
TCTGCAACTATTAATTCTTGGGATTATAATCAACAACAACCACAACCACAACAACCACAA
TCAGTATATAATAATCCATCATCATCAACCACAAATACAACTTACAATAATCCATCAACA
ACAACAACAACATCATCACCAACAAATACAAACACAACTTATAATAATCCATCATCAGCC
ACAAATACAAATTATAATAATAGAAATTCATGGGGATATGATCAATCAACTTATAATAAC
TCATCATATAATAATAATACTGATACATATTCAAATCAAACTCAACAACAAGAATATCAA
GTACTTTATGATAAAAAATATCAAGAAGAAGAAGAAAAGAGAAGAAAAGAATATGAAGAA
TCATGGAGAGTTTATTATGAACAACAAGCAGCTTATGAGAAAGAACAAGAAAGAATTAGA
AATGAAGAGATGGAAA----------AAGTTGAAGCGAGGGTTACAAAATTATTTAAAAG
TGAAGAAATTAATAAGGTATTTTCAAATTTAAAAAGTATCCTTCAAATTAGTACAGAGAT
TACTCATCTTTTAGAAGAAAGATTATCAGTTTCACCTGTTAAAATTGGTGATATTTTTGT
TAAATTTGCACCAATTTTTAAAATTTATGTGGAATATGTAAATAATTTTGATAAAGTAGC
ACCACAATTAAAAGCAATGACAGAGAATCCACAATCAAAGAAATTCTTTTCAGAACAAAG
AAATAAATCAAGAGTAGCAACTGATATTAATAGTTTATTAATTATGCCAGTTCAACGTTG
TCCAAGATATGAATTATTATTAAGGGAAATTTTAAAACACACTCAAGAGGATCATGTTGA
ATTTAAAAATATTAAAAGTGCTTATGAATCAATCAAAGATATTAATAAGTATATTAATGA
TAGAAAGAGAAATGTTGATAATAGAGCAAAATTATTAGAGTTACAAAAAGAAATTAAAAA
TGCACCAGAATTAATTGAATCTCATAGATATTTTGTTAGAGAGTCACAATGTAATATCTC
TGCAAATAAGAAAAGTGAAAGTGGTGGTTTTTATTTATTCTTTTTCAATGATATGGTGTT
AGTCACAAAGAAATCTAGCCTTTTTTCAAATTATAAATACGTTTACACAATTCCTTGAAA
AATGCAGACATTAAAGATATTAGTTCAAANATTCAATGATACGTATTATTGTGGTTCTCT
GAATCAAT
Length of connected seq. 1378
Full length Seq ID -
Full length Seq. -
Length of full length seq. -