NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI685
Library AF
(Link to library)
Clone ID AFI685
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13863-1
Original site URL
Representative seq. ID AFI685P
(Link to Original site)
Representative DNA sequence 
>AFI685 (AFI685Q) /CSM/AF/AFI6-D/AFI685Q.Seq.d/
ATTTTTTTTAAATAATAAATAAACATATACAATGATTGATTCAGATATAGAATTAAATAA
AATAATAGTAGTACCATTAATATCAAAAGATTCATTAGATATTAATGATGCAAAAAATTA
CTATGGAACCGAGTTATACCCAAATCAACCAATTCCAACCATAGTTTCAGCTCAATCTGA
AGTAGATTCTTTAATTAATCAAACTTCTGGTAATAATGATGAAGAAAGATTAGTTTTATT
ATTAAAACAACAACAACAAGAGGTAGAAAAAGATAAAACATTTGATACAAATAAATATTT
TAACGAATTATCAACAATTTTATTTGGAAAGAATTTAATTCATTCTGAAGTAATTAGTTC
AACACAAGATATAATGTTAAAATATTTAACATATACTCGTCAAGGATTAGTAATGATTGC
AGACCAACAAACCAAAGGTAGAGGACGTGGTGTAAATAAATTCTTGTCTCCATTGGGATG
TTTGTTAATGTCATTCAAATGTAAACAAACTGATGGTAATAAATTACCATTCCTTCAATA
TTTGGCTGGTATGGCAATGGTCGAAGCAATTCACTCTTTCCCAAGTGCATCTGATTTAXX
XXXXXXXXATGAAAGTTGGTGGTGTCCTCTGTGAATCAAATTATNTTAACAATGAATTTG
ATGTTGTCATTGGNGNTGGTGTTAATCTTTCAAATTCAAATAATCCATCAACAACTATNA
ATCAATTAATTCAAAANAAAAAATATGGATTTTCATCATCATCATTGGCAACAAGNACTG
ATATTCCNATTTATATTAGTANANAANAATTGGTTTCAAAATTNTTTAACAAATTNGAAC
CAATGTTTATGGAATTTACAANANATGGCTTTAATGCAGATNTTGAAAATANATACNCTG
ACTTATGGATGCATACAAATCNAATTGTAAAATTTAAANAAANAGATAACCNTCATGTTA
AAATCNTTGGNATTTCTGANAGTGGNTTCCTTAAAGCTATTGAATGTGATGCTGATGGTA
ANACATCTTCTGACTCTGAAACCGNTGAGCTTCNCCCAGATGGNACTTCNTTTGATATTC
AAAACTTAATTTTAANGCNAAAATCCCNACNACCNAAATAGATAAAAAAAAAAAAATTTT
TAGTAATCACTTTTTTTCCCAATATTTTCTTTACAGAATAATAAATAATAAATAAATAAT
AAATAAATAATAAATATATATATTTTTTATCA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
fflnnk*TYTMIDSDIELNKIIVVPLISKDSLDINDAKNYYGTELYPNQPIPTIVSAQSE
VDSLINQTSGNNDEERLVLLLKQQQQEVEKDKTFDTNKYFNELSTILFGKNLIHSEVISS
TQDIMLKYLTYTRQGLVMIADQQTKGRGRGVNKFLSPLGCLLMSFKCKQTDGNKLPFLQY
LAGMAMVEAIHSFPSASDL---

---MKVGGVLCESNYXNNEFDVVIGXGVNLSNSNNPSTTXNQLIQXKKYGFSSSSLATXT
DIPIYISXXXLVSKXFNKXEPMFMEFTXXGFNADXENXYXDLWMHTNXIVKFKXXDNXHV
KIXGISXSGFLKAIECDADGXTSSDSETXELXPDGTSFDIQNLILXXKSXXPK*ikkkkf
lvitffpniffte**iink**innkyiyfls
Translated Amino Acid sequence (All Frames) 
Frame A:
iffk**iniynd*fryrik*nnsstinikrfiry**ckkllwnrvipkstnsnhsfssi*
srffn*snfw****rkisfiikttttrgrkr*ni*yk*if*riinnfiwkefnsf*sn*f
ntrynvkifniyssrisndcrptnqr*rtwck*ilvsigmfvnviqm*tn*w**itipsi
fgwygngrsnslfpkci*f---

---MKVGGVLCESNYXNNEFDVVIGXGVNLSNSNNPSTTXNQLIQXKKYGFSSSSLATXT
DIPIYISXXXLVSKXFNKXEPMFMEFTXXGFNADXENXYXDLWMHTNXIVKFKXXDNXHV
KIXGISXSGFLKAIECDADGXTSSDSETXELXPDGTSFDIQNLILXXKSXXPK*ikkkkf
lvitffpniffte**iink**innkyiyfls

Frame B:
fflnnk*TYTMIDSDIELNKIIVVPLISKDSLDINDAKNYYGTELYPNQPIPTIVSAQSE
VDSLINQTSGNNDEERLVLLLKQQQQEVEKDKTFDTNKYFNELSTILFGKNLIHSEVISS
TQDIMLKYLTYTRQGLVMIADQQTKGRGRGVNKFLSPLGCLLMSFKCKQTDGNKLPFLQY
LAGMAMVEAIHSFPSASDL---

---*klvvssvnqixltmnlmlslxxvlifqiqiihqqlxin*fkxknmdfhhhhwqqxl
ifxfilvxxnwfqnxltnxnqclwnlqxmalmqxlkixtltygciqixl*nlxkxitxml
kslxflxvxslkllnvmlmvxhlltlkpxsfxqmxlxlifkt*f*xxnpxxxnr*kkknf
**slffpifslqnnk**innk*iiniyify

Frame C:
ff*iinkhiq*liqi*n*ik***yh*yqkih*ilmmqkitmepsytqinqfqp*fqlnlk
*il*likllvimmkkd*fyy*nnnnkr*kkikhliqiniltnyqqfyleri*filk*lvq
hki*c*ni*hilvkd***lqtnkpkvedvv*insclhwdvc*chsnvnklmvinyhsfni
wlvwqwskqftlsqvhli---

---eswwcpl*iklx*q*i*cchwxwc*sfkfk*sinnyxsinskxkiwifiiiignkx*
ysxly*xxxigfkix*qixtnvygiyxxwl*crx*kxix*lmdayksncki*xxr*psc*
nxwxf*xwxp*sy*m*c*w*xif*l*nr*asprwxfx*ysklnfxakipttxidkkkkif
snhffsqyflyriinnk*iink**iyiffi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI685 (AFI685Q) /CSM/AF/AFI6-D/AFI685Q.Seq.d/                       1826   0.0
SLA592 (SLA592Q) /CSM/SL/SLA5-D/SLA592Q.Seq.d/                       1053   0.0
SFJ562 (SFJ562Q) /CSM/SF/SFJ5-C/SFJ562Q.Seq.d/                       1053   0.0
SFD294 (SFD294Q) /CSM/SF/SFD2-D/SFD294Q.Seq.d/                       1053   0.0
AFL119 (AFL119Q) /CSM/AF/AFL1-A/AFL119Q.Seq.d/                        993   0.0
AFJ338 (AFJ338Q) /CSM/AF/AFJ3-B/AFJ338Q.Seq.d/                        896   0.0
AFL454 (AFL454Q) /CSM/AF/AFL4-C/AFL454Q.Seq.d/                        894   0.0
AFM643 (AFM643Q) /CSM/AF/AFM6-B/AFM643Q.Seq.d/                        864   0.0
AFN413 (AFN413Q) /CSM/AF/AFN4-A/AFN413Q.Seq.d/                        807   0.0
AFH395 (AFH395Q) /CSM/AF/AFH3-D/AFH395Q.Seq.d/                        805   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    46   4e-09  13
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   3e-08  14
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    38   4e-08  17
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    32   1e-06  14
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    34   5e-05  14
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          38   6e-05  26
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   1e-04  15
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   1e-04  9
AE014842|AE014842.1 Plasmodium falciparum 3D7 chromosome 11 section 7 of 8 of the complete sequence.    36   2e-04  14
AC115607|AC115607.2 Dictyostelium discoideum chromosome 2 map 4559693-4624405 strain AX4, complete sequence.    32   2e-04  10
dna update 2004. 9.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP008208_425(AP008208|pid:none) Oryza sativa (japonica cultivar-...    76   3e-23
AC006053_17(AC006053|pid:none) Arabidopsis thaliana chromosome 2...    75   2e-22
G84651(G84651)biotin holocarboxylase synthetase [imported] - Ara...    75   1e-20
CP000582_324(CP000582|pid:none) Ostreococcus lucimarinus CCE9901...    76   7e-20
(P50747) RecName: Full=Biotin--protein ligase;          EC=6.3.4...    76   2e-19
BC153857_1(BC153857|pid:none) Bos taurus similar to holocarboxyl...    76   1e-17
AP005804_18(AP005804|pid:none) Oryza sativa Japonica Group genom...    64   1e-17
(Q920N2) RecName: Full=Biotin--protein ligase;          EC=6.3.4...    75   2e-17
AK168925_1(AK168925|pid:none) Mus musculus 16 days embryo kidney...    75   2e-17
AC007505_1(AC007505|pid:none) Arabidopsis thaliana chromosome I ...    79   4e-17
protein update 2009. 4.23
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFI685 is cyt
5' end seq. ID AFI685F
5' end seq. 
>AFI685F.Seq
ATTTTTTTTAAATAATAAATAAACATATACAATGATTGATTCAGATATAGAATTAAATAA
AATAATAGTAGTACCATTAATATCAAAAGATTCATTAGATATTAATGATGCAAAAAATTA
CTATGGAACCGAGTTATACCCAAATCAACCAATTCCAACCATAGTTTCAGCTCAATCTGA
AGTAGATTCTTTAATTAATCAAACTTCTGGTAATAATGATGAAGAAAGATTAGTTTTATT
ATTAAAACAACAACAACAAGAGGTAGAAAAAGATAAAACATTTGATACAAATAAATATTT
TAACGAATTATCAACAATTTTATTTGGAAAGAATTTAATTCATTCTGAAGTAATTAGTTC
AACACAAGATATAATGTTAAAATATTTAACATATACTCGTCAAGGATTAGTAATGATTGC
AGACCAACAAACCAAAGGTAGAGGACGTGGTGTAAATAAATTCTTGTCTCCATTGGGATG
TTTGTTAATGTCATTCAAATGTAAACAAACTGATGGTAATAAATTACCATTCCTTCAATA
TTTGGCTGGTATGGCAATGGTCGAAGCAATTCACTCTTTCCCAAGTGCATCTGATTTA--
--------
Length of 5' end seq. 598
3' end seq. ID AFI685Z
3' end seq. 
>AFI685Z.Seq
----------ATGAAAGTTGGTGGTGTCCTCTGTGAATCAAATTATNTTAACAATGAATT
TGATGTTGTCATTGGNGNTGGTGTTAATCTTTCAAATTCAAATAATCCATCAACAACTAT
NAATCAATTAATTCAAAANAAAAAATATGGATTTTCATCATCATCATTGGCAACAAGNAC
TGATATTCCNATTTATATTAGTANANAANAATTGGTTTCAAAATTNTTTAACAAATTNGA
ACCAATGTTTATGGAATTTACAANANATGGCTTTAATGCAGATNTTGAAAATANATACNC
TGACTTATGGATGCATACAAATCNAATTGTAAAATTTAAANAAANAGATAACCNTCATGT
TAAAATCNTTGGNATTTCTGANAGTGGNTTCCTTAAAGCTATTGAATGTGATGCTGATGG
TAANACATCTTCTGACTCTGAAACCGNTGAGCTTCNCCCAGATGGNACTTCNTTTGATAT
TCAAAACTTAATTTTAANGCNAAAATCCCNACNACCNAAATAGATAAAAAAAAAAAAATT
TTTAGTAATCACTTTTTTTCCCAATATTTTCTTTACAGAATAATAAATAATAAATAAATA
ATAAATAAATAATAAATATATATATTTTTTATCA
Length of 3' end seq. 624
Connected seq. ID AFI685P
Connected seq. 
>AFI685P.Seq
ATTTTTTTTAAATAATAAATAAACATATACAATGATTGATTCAGATATAGAATTAAATAA
AATAATAGTAGTACCATTAATATCAAAAGATTCATTAGATATTAATGATGCAAAAAATTA
CTATGGAACCGAGTTATACCCAAATCAACCAATTCCAACCATAGTTTCAGCTCAATCTGA
AGTAGATTCTTTAATTAATCAAACTTCTGGTAATAATGATGAAGAAAGATTAGTTTTATT
ATTAAAACAACAACAACAAGAGGTAGAAAAAGATAAAACATTTGATACAAATAAATATTT
TAACGAATTATCAACAATTTTATTTGGAAAGAATTTAATTCATTCTGAAGTAATTAGTTC
AACACAAGATATAATGTTAAAATATTTAACATATACTCGTCAAGGATTAGTAATGATTGC
AGACCAACAAACCAAAGGTAGAGGACGTGGTGTAAATAAATTCTTGTCTCCATTGGGATG
TTTGTTAATGTCATTCAAATGTAAACAAACTGATGGTAATAAATTACCATTCCTTCAATA
TTTGGCTGGTATGGCAATGGTCGAAGCAATTCACTCTTTCCCAAGTGCATCTGATTTA--
--------ATGAAAGTTGGTGGTGTCCTCTGTGAATCAAATTATNTTAACAATGAATTTG
ATGTTGTCATTGGNGNTGGTGTTAATCTTTCAAATTCAAATAATCCATCAACAACTATNA
ATCAATTAATTCAAAANAAAAAATATGGATTTTCATCATCATCATTGGCAACAAGNACTG
ATATTCCNATTTATATTAGTANANAANAATTGGTTTCAAAATTNTTTAACAAATTNGAAC
CAATGTTTATGGAATTTACAANANATGGCTTTAATGCAGATNTTGAAAATANATACNCTG
ACTTATGGATGCATACAAATCNAATTGTAAAATTTAAANAAANAGATAACCNTCATGTTA
AAATCNTTGGNATTTCTGANAGTGGNTTCCTTAAAGCTATTGAATGTGATGCTGATGGTA
ANACATCTTCTGACTCTGAAACCGNTGAGCTTCNCCCAGATGGNACTTCNTTTGATATTC
AAAACTTAATTTTAANGCNAAAATCCCNACNACCNAAATAGATAAAAAAAAAAAAATTTT
TAGTAATCACTTTTTTTCCCAATATTTTCTTTACAGAATAATAAATAATAAATAAATAAT
AAATAAATAATAAATATATATATTTTTTATCA
Length of connected seq. 1222
Full length Seq ID -
Full length Seq. -
Length of full length seq. -