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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL469
Library SF
(Link to library)
Clone ID SFL469
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16523-1
Original site URL
Representative seq. ID SFL469P
(Link to Original site)
Representative DNA sequence 
>SFL469 (SFL469Q) /CSM/SF/SFL4-C/SFL469Q.Seq.d/
GTGCTTCACTTGATAGTGTAAATTTTGGTAGAGGCGGTGGCTGGGGAAGTGGAAACCAAG
GTGTACTTTGTGGAACACATTATTGTCCACCAGGTTCAACTTGTGAAAGTAAACATGGTC
ACTATATTTGTAGACCAGGTGGTTTAATAACTGCTCCAAGTGGTGGTGATAGTGGTGGTA
TTTGGACAGGTGGTGATAAACATAAAAAAGATTGTGGTGGCACAGGATGTTGTAAAGAAG
GTCAATATTGTACCAAACTTGATGGAAAAGAACAATGTGTATATTATCCAGATGACAAAG
GTCCACAATGTGGTGGTTCATTCTGTAGAGAAGGTGAAATTTGTGTTTTAGAACATGGTG
TTTTGGGTTGTCTTGAAAATCCAGTTGAAGATAGTAAAGCAAATTGTGGNTTATTCCATT
GTAATTACAATGAATATTGXXXXXXXXXXTGCTAAANCAGTTCAACATAANGCCCAACAA
CAACATGCCAAACCAGCTCAACATGCCGCTCACCAACAACATGCCAANCCAGCTCAACAT
GTCGCTCAACATCAACAACANCANCACGCCAAACCAGCTGCTCATACTGCTGCCAAACCA
GTTCAACATAATGCCCAACAACAACATGCTAAACCAGCTGCTCATACTGCTGCCAAACCA
GTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCCCATGGCGCCAAACCAGCT
GTTAATGCTGCCAGACCAGTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCT
CATACTGCTGCCAAACCAGTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCC
AAACCAGCCAAATTAACCAATGCCCAAAAATTACAACAAGAACACCAAAGACAAGAAGCT
CTCGCAAAACAACAAGCTCGTATTAGACAACAAAATCTTGTCAAACAAGCTGCCCAAAAG
AAACAAACCTCTCAACGTGCTGCCTCTAAAAATCAAGCTAGACCAGCAACTCAAAAGAGA
AATTAAATTTATTATTTAGTATAATTATACAATTGAAAAATTATAAATTGAGATTTCTTT
TTGTTGGTGATAGGTATTCAATAGATACACGAACTTTCAAATCATTTTTCAATTTCTTTA
TTTACCAATTGTTAAAACTCATTTTATTTATTTTTTCTCTTACAATCTCTTTTTCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ASLDSVNFGRGGGWGSGNQGVLCGTHYCPPGSTCESKHGHYICRPGGLITAPSGGDSGGI
WTGGDKHKKDCGGTGCCKEGQYCTKLDGKEQCVYYPDDKGPQCGGSFCREGEICVLEHGV
LGCLENPVEDSKANCGLFHCNYNEY---

---AKXVQHXAQQQHAKPAQHAAHQQHAXPAQHVAQHQQXXHAKPAAHTAAKPVQHNAQQ
QHAKPAAHTAAKPVQHNAAQQHAKPAAHGAKPAVNAARPVQHNAAQQHAKPAAHTAAKPV
QHNAAQQHAKPAAKPAKLTNAQKLQQEHQRQEALAKQQARIRQQNLVKQAAQKKQTSQRA
ASKNQARPATQKRN*iyylv*lyn*kiin*dffllvigiq*ihelsnhfsislftnc*ns
fylffllqslf
Translated Amino Acid sequence (All Frames) 
Frame A:
vlhliv*ilveavagevetkvyfvehiivhqvqlvkvnmvtifvdqvv**llqvvvivvv
fgqvvinikkivvaqdvvkkvnivpnlmeknnvyiiqmtkvhnvvvhsvekvkfvf*nmv
fwvvlkiqlkivkqivxysivitmni---

---c*xsst*xptttcqtsstcrspttcqxsstcrststtxxrqtscsyccqtsst*cpt
ttc*tscsyccqtsstqccsttc*tscpwrqtsc*ccqtsstqccsttc*tscsyccqts
stqccsttc*tscqtsqinqcpkittrtpktrssrkttssy*ttkscqtscpketnlstc
cl*kss*tsnskeklnllfsiiiqlknyklrflfvgdrysidtrtfksffnffiyqllkl
ilfifsltisfs

Frame B:
cft**ckfw*rrwlgkwkprctlwntllstrfnl*k*twslyl*trwfnncskww**wwy
ldrw**t*krlwwhrml*rrsilyqt*wkrtmcilsr*qrstmwwfil*rr*nlcfrtwc
fgls*kss*r**sklwxipl*lq*il---

---AKXVQHXAQQQHAKPAQHAAHQQHAXPAQHVAQHQQXXHAKPAAHTAAKPVQHNAQQ
QHAKPAAHTAAKPVQHNAAQQHAKPAAHGAKPAVNAARPVQHNAAQQHAKPAAHTAAKPV
QHNAAQQHAKPAAKPAKLTNAQKLQQEHQRQEALAKQQARIRQQNLVKQAAQKKQTSQRA
ASKNQARPATQKRN*iyylv*lyn*kiin*dffllvigiq*ihelsnhfsislftnc*ns
fylffllqslf

Frame C:
ASLDSVNFGRGGGWGSGNQGVLCGTHYCPPGSTCESKHGHYICRPGGLITAPSGGDSGGI
WTGGDKHKKDCGGTGCCKEGQYCTKLDGKEQCVYYPDDKGPQCGGSFCREGEICVLEHGV
LGCLENPVEDSKANCGLFHCNYNEY---

---lxqfnixpnnnmpnqlnmpltnnmpxqlnmslninnxxtpnqllillpnqfnimpnn
nmlnqllillpnqfntmllnnmlnqlpmapnqllmlpdqfntmllnnmlnqllillpnqf
ntmllnnmlnqlpnqpn*pmpknynkntkdkklsqnnklvldnkilsnklpkrnkplnvl
plkikldqqlkreikfii*ynytiekl*ieisfcw**vfnrytnfqiifqflylpivkth
fiyffsynlff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL469 (SFL469Q) /CSM/SF/SFL4-C/SFL469Q.Seq.d/                       2127   0.0
CFJ668 (CFJ668Q) /CSM/CF/CFJ6-C/CFJ668Q.Seq.d/                       1287   0.0
CFA448 (CFA448Q) /CSM/CF/CFA4-B/CFA448Q.Seq.d/                       1287   0.0
CFA402 (CFA402Q) /CSM/CF/CFA4-A/CFA402Q.Seq.d/                       1287   0.0
SFH246 (SFH246Q) /CSM/SF/SFH2-B/SFH246Q.Seq.d/                       1281   0.0
SFJ585 (SFJ585Q) /CSM/SF/SFJ5-D/SFJ585Q.Seq.d/                       1279   0.0
CFI839 (CFI839Q) /CSM/CF/CFI8-B/CFI839Q.Seq.d/                       1277   0.0
AHF579 (AHF579Q) /CSM/AH/AHF5-D/AHF579Q.Seq.d/                       1275   0.0
SFE454 (SFE454Q) /CSM/SF/SFE4-C/SFE454Q.Seq.d/                       1273   0.0
AHJ722 (AHJ722Q) /CSM/AH/AHJ7-A/AHJ722Q.Seq.d/                       1273   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.  1287   0.0    14
X57149|X57149.1 D.discoideum EB4 gene for prespore vesicle protein.   819   0.0    12
M11028|M11028.1 Slime mold (D.discoideum) prespore EB4 gene.      212   e-131  6
BU714294|BU714294.1 SJAACBF11 Adult SjC 7/94 Schistosoma japonicum cDNA, mRNA sequence.    34   5e-04  3
BX842673|BX842673.1 Mouse DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP23-53F17.    38   0.007  6
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   0.008  16
AC131787|AC131787.3 Mus musculus BAC clone RP24-397K14 from chromosome 18, complete sequence.    36   0.013  7
AC100919|AC100919.1 Mus musculus clone RP23-71H20, LOW-PASS SEQUENCE SAMPLING.    40   0.047  4
AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    32   0.054  8
AC120429|AC120429.4 Mus musculus chromosome 18 clone RP24-546J19 map 18, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    36   0.059  7
dna update 2004. 1. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

S28358(S28358;S20090) prespore vesicle protein - slime mold (Dic...   268   2e-70
(P08798) RecName: Full=Prespore vesicle protein;          Short=...   268   2e-70
(O70244) RecName: Full=Cubilin; AltName: Full=Intrinsic factor-c...    44   0.014
(Q9JLB4) RecName: Full=Cubilin; AltName: Full=Intrinsic factor-c...    42   0.032
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    42   0.042
(Q9TU53) RecName: Full=Cubilin; Flags: Precursor;  &AF137068_1(A...    41   0.093
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        39   0.27
(Q7ZXL5) RecName: Full=Protein kinase C-binding protein NELL2; A...    39   0.46
(O73775) RecName: Full=Fibulin-1;          Short=FIBL-1; Flags: ...    38   0.60
(Q54G96) RecName: Full=Uncharacterized protein DDB_G0290301;           38   0.60
protein update 2009. 5.28
PSORT 

 psg: 0.84  gvh: 0.52  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.11  mip: 0.08
 nuc: 0.02  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SFL469 is cyt
5' end seq. ID SFL469F
5' end seq. 
>SFL469F.Seq
GTGCTTCACTTGATAGTGTAAATTTTGGTAGAGGCGGTGGCTGGGGAAGTGGAAACCAAG
GTGTACTTTGTGGAACACATTATTGTCCACCAGGTTCAACTTGTGAAAGTAAACATGGTC
ACTATATTTGTAGACCAGGTGGTTTAATAACTGCTCCAAGTGGTGGTGATAGTGGTGGTA
TTTGGACAGGTGGTGATAAACATAAAAAAGATTGTGGTGGCACAGGATGTTGTAAAGAAG
GTCAATATTGTACCAAACTTGATGGAAAAGAACAATGTGTATATTATCCAGATGACAAAG
GTCCACAATGTGGTGGTTCATTCTGTAGAGAAGGTGAAATTTGTGTTTTAGAACATGGTG
TTTTGGGTTGTCTTGAAAATCCAGTTGAAGATAGTAAAGCAAATTGTGGNTTATTCCATT
GTAATTACAATGAATATTG----------
Length of 5' end seq. 439
3' end seq. ID SFL469Z
3' end seq. 
>SFL469Z.Seq
----------TGCTAAANCAGTTCAACATAANGCCCAACAACAACATGCCAAACCAGCTC
AACATGCCGCTCACCAACAACATGCCAANCCAGCTCAACATGTCGCTCAACATCAACAAC
ANCANCACGCCAAACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACATAATGCCCAAC
AACAACATGCTAAACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACACAATGCTGCTC
AACAACATGCTAAACCAGCTGCCCATGGCGCCAAACCAGCTGTTAATGCTGCCAGACCAG
TTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCTCATACTGCTGCCAAACCAG
TTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCCAAACCAGCCAAATTAACCA
ATGCCCAAAAATTACAACAAGAACACCAAAGACAAGAAGCTCTCGCAAAACAACAAGCTC
GTATTAGACAACAAAATCTTGTCAAACAAGCTGCCCAAAAGAAACAAACCTCTCAACGTG
CTGCCTCTAAAAATCAAGCTAGACCAGCAACTCAAAAGAGAAATTAAATTTATTATTTAG
TATAATTATACAATTGAAAAATTATAAATTGAGATTTCTTTTTGTTGGTGATAGGTATTC
AATAGATACACGAACTTTCAAATCATTTTTCAATTTCTTTATTTACCAATTGTTAAAACT
CATTTTATTTATTTTTTCTCTTACAATCTCTTTTTCA
Length of 3' end seq. 747
Connected seq. ID SFL469P
Connected seq. 
>SFL469P.Seq
GTGCTTCACTTGATAGTGTAAATTTTGGTAGAGGCGGTGGCTGGGGAAGTGGAAACCAAG
GTGTACTTTGTGGAACACATTATTGTCCACCAGGTTCAACTTGTGAAAGTAAACATGGTC
ACTATATTTGTAGACCAGGTGGTTTAATAACTGCTCCAAGTGGTGGTGATAGTGGTGGTA
TTTGGACAGGTGGTGATAAACATAAAAAAGATTGTGGTGGCACAGGATGTTGTAAAGAAG
GTCAATATTGTACCAAACTTGATGGAAAAGAACAATGTGTATATTATCCAGATGACAAAG
GTCCACAATGTGGTGGTTCATTCTGTAGAGAAGGTGAAATTTGTGTTTTAGAACATGGTG
TTTTGGGTTGTCTTGAAAATCCAGTTGAAGATAGTAAAGCAAATTGTGGNTTATTCCATT
GTAATTACAATGAATATTG----------TGCTAAANCAGTTCAACATAANGCCCAACAA
CAACATGCCAAACCAGCTCAACATGCCGCTCACCAACAACATGCCAANCCAGCTCAACAT
GTCGCTCAACATCAACAACANCANCACGCCAAACCAGCTGCTCATACTGCTGCCAAACCA
GTTCAACATAATGCCCAACAACAACATGCTAAACCAGCTGCTCATACTGCTGCCAAACCA
GTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCCCATGGCGCCAAACCAGCT
GTTAATGCTGCCAGACCAGTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCT
CATACTGCTGCCAAACCAGTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCC
AAACCAGCCAAATTAACCAATGCCCAAAAATTACAACAAGAACACCAAAGACAAGAAGCT
CTCGCAAAACAACAAGCTCGTATTAGACAACAAAATCTTGTCAAACAAGCTGCCCAAAAG
AAACAAACCTCTCAACGTGCTGCCTCTAAAAATCAAGCTAGACCAGCAACTCAAAAGAGA
AATTAAATTTATTATTTAGTATAATTATACAATTGAAAAATTATAAATTGAGATTTCTTT
TTGTTGGTGATAGGTATTCAATAGATACACGAACTTTCAAATCATTTTTCAATTTCTTTA
TTTACCAATTGTTAAAACTCATTTTATTTATTTTTTCTCTTACAATCTCTTTTTCA
Length of connected seq. 1186
Full length Seq ID -
Full length Seq. -
Length of full length seq. -