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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL448
Library SF
(Link to library)
Clone ID SFL448
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14948-1
Original site URL
Representative seq. ID SFL448P
(Link to Original site)
Representative DNA sequence 
>SFL448 (SFL448Q) /CSM/SF/SFL4-B/SFL448Q.Seq.d/
GATTTATCTTTATCTTACTTTTTACCATACAATTNAACTTTTTTGTATAGTTTTTTATAA
TTTTAAAGAAAAAATAAAAAGATGTCAGCTGTTGATGATCAACAAAATGCAGATAAGCAA
GTTGAACAATGGAAAATCAAAAAATTAATTAAAAATTTGGAAGCTGCCAGAGGTAATGGT
ACATCTATGATTTCATTAATTATTCGTCCAGGTGATCAAATTGCAAAAGTTAATAAAATG
TTAGCAGAAGAGTATGGTACAGCATCAAATATTAAATCAAGAGTTAATAGATTATCAGTA
TTGGGTGCAATTACATCAGCACAACAAAGATTAAAGTTATATACTAAAGTACCAGATAAT
GGTTTAGTTATTTATTGCGGTACAATGGTTACAGATGAAGGTAAAGAGAAACCAGTACGT
ATCGATATTGAACCATTCAAACCAATCAATACCTCACTCTATTTATGTGATAATAAATTC
CATACAGCACCATTGGGTGAATTATTAGAGAGTGATGAAAAGTTTGGTTTCATCCGTCGT
AGATGGTAATGGTGCACTCXXXXXXXXXXTCAAGTGTCAAATTCATTCAAGAGAAGAAAT
TAATCTCACAATTCCTTTGAAGAGATCGCACAAGACACTGGTAAGTATTGTTTCGGTATC
GCTGATACTTTGAAAGCTTTGGACTTGGGTGCTGCTCACACTTTAATCGTTTGGGAATCA
TTGGAAACAATTCGTTACTTACTTCGTTTACCAACTGGTGAAGAGAAAGTCATCTTTTTA
AATAAAGATCAAAATAAAGATGCCTCTGTCTTTAAGGATAAAGAATCTGGTCTTGACTAT
GAAATCGTCGAAGAAATGCCAATCGTTGAGTGGTTCGCCAATAACTACAAAAACTTTGGT
GCCTCTCTCGAATTCGTCACCAACAAATCTCAAGAAGGTTCACAATTCTGTAAAGGTTTT
GGTGGTTTGGGTGGTTTACTCCGTTATCAAGTAGATTTCGCTCAACTCAATGATTTCGAT
AATCCAGATGAGAATGAATACGATGATAGTGATTCAGACTTTTGACCAATTGAAAAAACC
AGCAACAGAAATGGTACTGGTTTGGTCTCCTCCACTTTTAAGGTTGCCCCTTCCTTCTCT
ACCATTCAAAAACAACAACAACAACAACAAAAATCACTTTCACCAAAAACAACATTAACA
ACAACTATTACAACAACTACAACAACAACAACAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iylyltfyhtixlfcivfynfkekikrcqllminkmqisklnngkskn*lkiwklpevmv
hl*fh*lfvqviklqklikc*qksmvqhqilnqelidyqywvqlhqhnkd*syilkyqim
v*lfiavqwlqmkvkrnqyvsilnhsnqsiphsiyviinsiqhhwvny*RVMKSLVSSVV
DGNGAL---

---KCQIHSREEINLTIPLKRSHKTLVSIVSVSLIL*klwtwvlltl*sfgnhwkqfvty
fvyqlvkrkssf*ikikikmplslriknlvltmksskkcqslsgspittktlvplsnssp
tnlkkvhnsvkvlvvwvvysvik*islnsmisiiqmrmntmiviqtfdqlkkpatemvlv
wsppllrlplpslpfknnnnnnknhfhqkqh*qqllqqlqqqqq
Translated Amino Acid sequence (All Frames) 
Frame A:
dlslsyflpynxtflysfl*f*rknkkmsavddqqnadkqveqwkikkliknleaargng
tsmisliirpgdqiakvnkmlaeeygtasniksrvnrlsvlgaitsaqqrlklytkvpdn
glviycgtmvtdegkekpvridiepfkpintslylcdnkfhtaplgellesdekfgfirr
rw*wct---

---ssvkfiqekklisqfl*rdrtrhw*vlfryr*yfesfglgccshfnrlgiignnsll
tsftnw*reshlfk*rsk*rclcl*g*riws*l*nrrrnanr*vvrq*lqklwclsrirh
qqisrrftil*rfwwfgwftplssrfrstq*fr*sr*e*ir***frlltn*knqqqkwyw
fgllhf*gcpfllyhskttttttkitftknninnnyynnynnnn

Frame B:
iylyltfyhtixlfcivfynfkekikrcqllminkmqisklnngkskn*lkiwklpevmv
hl*fh*lfvqviklqklikc*qksmvqhqilnqelidyqywvqlhqhnkd*syilkyqim
v*lfiavqwlqmkvkrnqyvsilnhsnqsiphsiyviinsiqhhwvny*RVMKSLVSSVV
DGNGAL---

---qvsnsfkrrn*shnsfeeiaqdtgkycfgiadtlkaldlgaahtlivwesletiryl
lrlptgeekviflnkdqnkdasvfkdkesgldyeiveempivewfannyknfgaslefvt
nksqegsqfckgfgglggllryqvdfaqlndfdnpdeneyddsdsdf*piektsnrngtg
lvsstfkvapsfstiqkqqqqqqkslspkttltttitttttttt

Frame C:
fifillftiqxnffv*ffiilkkk*kdvsc**stkcr*as*tmenqkin*kfgscqr*wy
iydfinyssr*sncks**nvsrrvwysiky*iks**iisigcnyisttkikviy*str*w
fsyllryngyr*r*retstyry*tiqtnqyltlfm***ipystig*iire**kvwfhps*
mvmvh---

---KCQIHSREEINLTIPLKRSHKTLVSIVSVSLIL*klwtwvlltl*sfgnhwkqfvty
fvyqlvkrkssf*ikikikmplslriknlvltmksskkcqslsgspittktlvplsnssp
tnlkkvhnsvkvlvvwvvysvik*islnsmisiiqmrmntmiviqtfdqlkkpatemvlv
wsppllrlplpslpfknnnnnnknhfhqkqh*qqllqqlqqqqq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL448 (SFL448Q) /CSM/SF/SFL4-B/SFL448Q.Seq.d/                       1901   0.0
VFM203 (VFM203Q) /CSM/VF/VFM2-A/VFM203Q.Seq.d/                       1124   0.0
VFK364 (VFK364Q) /CSM/VF/VFK3-C/VFK364Q.Seq.d/                       1124   0.0
VFI434 (VFI434Q) /CSM/VF/VFI4-B/VFI434Q.Seq.d/                       1124   0.0
CHG846 (CHG846Q) /CSM/CH/CHG8-B/CHG846Q.Seq.d/                       1124   0.0
AFH772 (AFH772Q) /CSM/AF/AFH7-C/AFH772Q.Seq.d/                       1124   0.0
AFE158 (AFE158Q) /CSM/AF/AFE1-C/AFE158Q.Seq.d/                       1124   0.0
AFD417 (AFD417Q) /CSM/AF/AFD4-A/AFD417Q.Seq.d/                       1120   0.0
VFO568 (VFO568Q) /CSM/VF/VFO5-C/VFO568Q.Seq.d/                       1116   0.0
VFK387 (VFK387Q) /CSM/VF/VFK3-D/VFK387Q.Seq.d/                       1116   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF298834|AF298834.1 Dictyostelium discoideum eukaryotic release factor 1 (erf1) mRNA, complete cds.  1043   0.0    3
X54055|X54055.1 X.laevis gene for suppressor.                      88   6e-24  4
Z14253|Z14253.1 X.laevis XLCL1 mRNA.                               88   7e-24  4
BC061387|BC061387.1 Silurana tropicalis cDNA clone MGC:75958 IMAGE:5384090, complete cds.    88   8e-23  5
BI095303|BI095303.1 dac03g10.y1 NICHD XGC Emb2 Xenopus laevis cDNA clone IMAGE:4406202 5' similar to SW:ERF1_HUMAN P46055 EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1 ;, mRNA sequence.    88   2e-19  2
BQ387670|BQ387670.1 NISC_mn25b04.y1 NICHD XGC Ov1 Xenopus laevis cDNA clone IMAGE:5074494 5', mRNA sequence.    88   2e-19  2
BJ623442|BJ623442.1 Xenopus laevis cDNA clone:XL204l03, 5' end.    88   2e-19  2
BI449943|BI449943.1 dac75e08.y1 RIKEN Xenopus egg Xenopus laevis cDNA clone IMAGE:4436054 5' similar to SW:ERF1_HUMAN P46055 EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1 ;, mRNA sequence.    88   2e-19  2
BQ384641|BQ384641.1 NISC_mn07h03.y1 NICHD XGC Ov1 Xenopus laevis cDNA clone IMAGE:5049460 5', mRNA sequence.    88   2e-19  2
BJ618767|BJ618767.1 Xenopus laevis cDNA clone:XL186h15, 5' end.    88   2e-19  2
dna update 2004. 1. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF298834_1(AF298834|pid:none) Dictyostelium discoideum eukaryoti...   300   5e-80
BT044018_1(BT044018|pid:none) Salmo salar clone HM5_1032 eukaryo...   234   5e-60
BC013717_1(BC013717|pid:none) Mus musculus eukaryotic translatio...   234   6e-60
DQ241835_1(DQ241835|pid:none) Solanum tuberosum clone 180C08 euk...   233   1e-59
(P62498) RecName: Full=Eukaryotic peptide chain release factor s...   233   1e-59
(P62495) RecName: Full=Eukaryotic peptide chain release factor s...   233   1e-59
(P35614) RecName: Full=Eukaryotic peptide chain release factor s...   232   2e-59
AF451327_1(AF451327|pid:none) Aspergillus nidulans eukaryotic po...   232   2e-59
DQ311356_1(DQ311356|pid:none) Bombyx mori eukaryotic translation...   231   5e-59
AB052893_1(AB052893|pid:none) Pneumocystis carinii mRNA for euka...   231   5e-59
protein update 2009. 5.28
PSORT 

 psg: 0.79  gvh: 0.49  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 12.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for SFL448 is nuc
5' end seq. ID SFL448F
5' end seq. 
>SFL448F.Seq
GATTTATCTTTATCTTACTTTTTACCATACAATTNAACTTTTTTGTATAGTTTTTTATAA
TTTTAAAGAAAAAATAAAAAGATGTCAGCTGTTGATGATCAACAAAATGCAGATAAGCAA
GTTGAACAATGGAAAATCAAAAAATTAATTAAAAATTTGGAAGCTGCCAGAGGTAATGGT
ACATCTATGATTTCATTAATTATTCGTCCAGGTGATCAAATTGCAAAAGTTAATAAAATG
TTAGCAGAAGAGTATGGTACAGCATCAAATATTAAATCAAGAGTTAATAGATTATCAGTA
TTGGGTGCAATTACATCAGCACAACAAAGATTAAAGTTATATACTAAAGTACCAGATAAT
GGTTTAGTTATTTATTGCGGTACAATGGTTACAGATGAAGGTAAAGAGAAACCAGTACGT
ATCGATATTGAACCATTCAAACCAATCAATACCTCACTCTATTTATGTGATAATAAATTC
CATACAGCACCATTGGGTGAATTATTAGAGAGTGATGAAAAGTTTGGTTTCATCCGTCGT
AGATGGTAATGGTGCACTC----------
Length of 5' end seq. 559
3' end seq. ID SFL448Z
3' end seq. 
>SFL448Z.Seq
----------TCAAGTGTCAAATTCATTCAAGAGAAGAAATTAATCTCACAATTCCTTTG
AAGAGATCGCACAAGACACTGGTAAGTATTGTTTCGGTATCGCTGATACTTTGAAAGCTT
TGGACTTGGGTGCTGCTCACACTTTAATCGTTTGGGAATCATTGGAAACAATTCGTTACT
TACTTCGTTTACCAACTGGTGAAGAGAAAGTCATCTTTTTAAATAAAGATCAAAATAAAG
ATGCCTCTGTCTTTAAGGATAAAGAATCTGGTCTTGACTATGAAATCGTCGAAGAAATGC
CAATCGTTGAGTGGTTCGCCAATAACTACAAAAACTTTGGTGCCTCTCTCGAATTCGTCA
CCAACAAATCTCAAGAAGGTTCACAATTCTGTAAAGGTTTTGGTGGTTTGGGTGGTTTAC
TCCGTTATCAAGTAGATTTCGCTCAACTCAATGATTTCGATAATCCAGATGAGAATGAAT
ACGATGATAGTGATTCAGACTTTTGACCAATTGAAAAAACCAGCAACAGAAATGGTACTG
GTTTGGTCTCCTCCACTTTTAAGGTTGCCCCTTCCTTCTCTACCATTCAAAAACAACAAC
AACAACAACAAAAATCACTTTCACCAAAAACAACATTAACAACAACTATTACAACAACTA
CAACAACAACAACAA
Length of 3' end seq. 665
Connected seq. ID SFL448P
Connected seq. 
>SFL448P.Seq
GATTTATCTTTATCTTACTTTTTACCATACAATTNAACTTTTTTGTATAGTTTTTTATAA
TTTTAAAGAAAAAATAAAAAGATGTCAGCTGTTGATGATCAACAAAATGCAGATAAGCAA
GTTGAACAATGGAAAATCAAAAAATTAATTAAAAATTTGGAAGCTGCCAGAGGTAATGGT
ACATCTATGATTTCATTAATTATTCGTCCAGGTGATCAAATTGCAAAAGTTAATAAAATG
TTAGCAGAAGAGTATGGTACAGCATCAAATATTAAATCAAGAGTTAATAGATTATCAGTA
TTGGGTGCAATTACATCAGCACAACAAAGATTAAAGTTATATACTAAAGTACCAGATAAT
GGTTTAGTTATTTATTGCGGTACAATGGTTACAGATGAAGGTAAAGAGAAACCAGTACGT
ATCGATATTGAACCATTCAAACCAATCAATACCTCACTCTATTTATGTGATAATAAATTC
CATACAGCACCATTGGGTGAATTATTAGAGAGTGATGAAAAGTTTGGTTTCATCCGTCGT
AGATGGTAATGGTGCACTC----------TCAAGTGTCAAATTCATTCAAGAGAAGAAAT
TAATCTCACAATTCCTTTGAAGAGATCGCACAAGACACTGGTAAGTATTGTTTCGGTATC
GCTGATACTTTGAAAGCTTTGGACTTGGGTGCTGCTCACACTTTAATCGTTTGGGAATCA
TTGGAAACAATTCGTTACTTACTTCGTTTACCAACTGGTGAAGAGAAAGTCATCTTTTTA
AATAAAGATCAAAATAAAGATGCCTCTGTCTTTAAGGATAAAGAATCTGGTCTTGACTAT
GAAATCGTCGAAGAAATGCCAATCGTTGAGTGGTTCGCCAATAACTACAAAAACTTTGGT
GCCTCTCTCGAATTCGTCACCAACAAATCTCAAGAAGGTTCACAATTCTGTAAAGGTTTT
GGTGGTTTGGGTGGTTTACTCCGTTATCAAGTAGATTTCGCTCAACTCAATGATTTCGAT
AATCCAGATGAGAATGAATACGATGATAGTGATTCAGACTTTTGACCAATTGAAAAAACC
AGCAACAGAAATGGTACTGGTTTGGTCTCCTCCACTTTTAAGGTTGCCCCTTCCTTCTCT
ACCATTCAAAAACAACAACAACAACAACAAAAATCACTTTCACCAAAAACAACATTAACA
ACAACTATTACAACAACTACAACAACAACAACAA
Length of connected seq. 1224
Full length Seq ID -
Full length Seq. -
Length of full length seq. -