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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL427
Library SF
(Link to library)
Clone ID SFL427
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09878-1
Original site URL
Representative seq. ID SFL427P
(Link to Original site)
Representative DNA sequence 
>SFL427 (SFL427Q) /CSM/SF/SFL4-B/SFL427Q.Seq.d/
CATCCTTTCTAAATCGAAATAACTTTAATTAATTAGGTTTTAGTTTATACAATATATAGG
GAGAAAATATATAATACAGAATGAGTCTTGTACAAAAAATCAAAGAGGTCGAGGATGAGA
TGGCTCGTACCCAAAAAAATAAAGCTACAAGCTTTCATTTAGGTGTATTGAAAGCAAAAT
TAGCAAAATATAAAAGAGAATTATTGTTAGGACCAAGTAAAGGTGCAGCAGCAGGTGCAG
GTGAAGGTTTCGATGTATCAAAAGCAGGTGATGCTCGTGTTGGTTTAATTGGTTTCCCAT
CAGTTGGTAAATCAACTTTATTAACTAAATTAACTGGTACCTCATCTGAAGTTGCCTCAT
ACGAATTCACAACTTTAACATGTATTCCTGGTGTAATTAATTATAAAGGTGCTAAAATTC
AATTATTAGATTTACCAGGTATTATTGAAGGTGCAAAAGATGGTAAAGGTAGAGGTXXXX
XXXXXXTAGAGAGAGAGTTGGATGGTTTTGGTATTAGATTGAATAAACAACCCACCACCA
ATCACATTCAAAAAGAAGGAGAAGGGTGGTATTAACTTTTCACATACACCAAATGTCAAT
CCAACCCAATTGGATAGTGAAACTGTGAAAGCAATCTGTGCAGAATATAAAATTCACAAT
GCAGATGTGATTTTACGTGGAAATTGTACAGTGGATGAATTCATCGATGTCATTGAAGGC
AATCGTATCTACGTTCCATGTATTTACGTTTTAAATAAGATCGATGCTATCTCTATCGAA
GAGTTGGACCTTTTAGATAAAATTCCTCATTATGTTCCAATCTCTTCTCATTTGGAATGG
AATTTAGATGCCCTCCTCGATAAAATTTGGGAATACTTAAAATTGATTAGAGTTTACACT
AAACCAAAAGGTCTCATTCCAGATTACAATGAACCAGTCGTCATTAGAGGTGGTGAAGAA
GCTTCAATTGAAACTTTTTGTAATCATATTCATAATTCAATTATTAGACAATTTAGATAT
GCTTTGGTTTGGGGTTCTTCAGCTAAACATAACCCTCAACGTTGTGGTAAGGATCACGTT
TTAGCAGATAAGATATGTTCAAATGTAAAAAATAAGATC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
sflnrnnfn*lgfslyni*geni*YRMSLVQKIKEVEDEMARTQKNKATSFHLGVLKAKL
AKYKRELLLGPSKGAAAGAGEGFDVSKAGDARVGLIGFPSVGKSTLLTKLTGTSSEVASY
EFTTLTCIPGVINYKGAKIQLLDLPGIIEGAKDGKGRG---

---ERELDGFGIRLNKQPTTNHIQKEGEGWY*lftytkcqsnpig**ncesnlcri*nsq
crcdftwklysg*ihrch*rqsylrsmylrfk*drcylyrrvgpfr*nsslcsnlfsfgm
efrcppr*nlgilkid*slh*tkrshsrlq*tsrh*rw*rsfn*nfl*sys*fny*ti*i
cfglgffs*t*pstlw*gsrfsr*dmfkckk*d
Translated Amino Acid sequence (All Frames) 
Frame A:
hpf*ieitlin*vlvytiyrekiynte*vlykkskrsrmrwlvpkkiklqafi*vy*kqn
*qnikenyc*dqvkvqqqvqvkvsmyqkqvmlvlv*lvshqlvnqly*ln*lvphlklph
tnsql*hvflv*liikvlkfny*iyqvllkvqkmvkve---

---*reswmvlvld*innpppitfkkkekgginfshtpnvnptqldsetvkaicaeykih
nadvilrgnctvdefidviegnriyvpciyvlnkidaisieeldlldkiphyvpisshle
wnldalldkiweylklirvytkpkglipdynepvvirggeeasietfcnhihnsiirqfr
yalvwgssakhnpqrcgkdhvladkicsnvknki

Frame B:
ilsksk*l*lirf*fiqyigrkyiiqnesctknqrgrg*dgsypkk*syklsfrcieski
ski*kriivrtk*rcssrcr*rfrciksr*cscwfnwfpisw*infin*inwyli*scli
rihnfnmyswcn*l*rc*nsiirftryy*rckrw*r*r---

---rervgwfwy*ie*tthhqshskrrrrvvltfhihqmsiqpnwivkl*kqsvqnikft
mqm*fyveivqwmnssmslkaivstfhvftf*irsmlslskswtf*ikflimfqslliwn
gi*mpssikfgnt*n*leftlnqkvsfqitmnqsslevvkklqlklfviifiiqlldnld
mlwfgvlqlnitlnvvvritf*qiryvqm*kir

Frame C:
sflnrnnfn*lgfslyni*geni*YRMSLVQKIKEVEDEMARTQKNKATSFHLGVLKAKL
AKYKRELLLGPSKGAAAGAGEGFDVSKAGDARVGLIGFPSVGKSTLLTKLTGTSSEVASY
EFTTLTCIPGVINYKGAKIQLLDLPGIIEGAKDGKGRG---

---ERELDGFGIRLNKQPTTNHIQKEGEGWY*lftytkcqsnpig**ncesnlcri*nsq
crcdftwklysg*ihrch*rqsylrsmylrfk*drcylyrrvgpfr*nsslcsnlfsfgm
efrcppr*nlgilkid*slh*tkrshsrlq*tsrh*rw*rsfn*nfl*sys*fny*ti*i
cfglgffs*t*pstlw*gsrfsr*dmfkckk*d
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL427 (SFL427Q) /CSM/SF/SFL4-B/SFL427Q.Seq.d/                       2117   0.0
VFA873 (VFA873Q) /CSM/VF/VFA8-D/VFA873Q.Seq.d/                       1225   0.0
VFB390 (VFB390Q) /CSM/VF/VFB3-D/VFB390Q.Seq.d/                       1219   0.0
VFO810 (VFO810Q) /CSM/VF/VFO8-A/VFO810Q.Seq.d/                       1205   0.0
SFD415 (SFD415Q) /CSM/SF/SFD4-A/SFD415Q.Seq.d/                       1179   0.0
VFN791 (VFN791Q) /CSM/VF/VFN7-D/VFN791Q.Seq.d/                       1178   0.0
SFF587 (SFF587Q) /CSM/SF/SFF5-D/SFF587Q.Seq.d/                       1178   0.0
SLB732 (SLB732Q) /CSM/SL/SLB7-B/SLB732Q.Seq.d/                       1160   0.0
VFI139 (VFI139Q) /CSM/VF/VFI1-B/VFI139Q.Seq.d/                       1112   0.0
AFB134 (AFB134Q) /CSM/AF/AFB1-B/AFB134Q.Seq.d/                       1110   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BM398238|BM398238.1 5009-0-42-F11.t.1 Chilcoat/Turkewitz cDNA (large fraction)Tetrahymena thermophila cDNA, mRNA sequence.    54   4e-29  6
BM167590|BM167590.1 EST570113 PyBS Plasmodium yoelii yoelii cDNA clone PYCON24 5' end, mRNA sequence.   137   4e-28  1
CK250070|CK250070.1 EST733707 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCBD21 5' end, mRNA sequence.    44   2e-24  6
BM401388|BM401388.1 5009-0-9-H05.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    52   5e-24  5
CK272276|CK272276.1 EST718354 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAD422 5' end, mRNA sequence.    44   5e-21  6
AQ854898|AQ854898.1 CpG2108B CpIOWAgDNA1 Cryptosporidium parvum genomic similar to developmentally regulated GTP-binding protein, DNA sequence.   103   5e-18  1
BQ515194|BQ515194.1 EST622609 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMIQ66 3' end, mRNA sequence.    44   6e-15  4
BX561726|BX561726.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse57d12_q1c.    62   1e-14  4
BM170653|BM170653.1 EST573176 PyBS Plasmodium yoelii yoelii cDNA clone PYCQJ35 5' end, mRNA sequence.    90   8e-14  1
CD988033|CD988033.1 QAO8a04.yg QAO Zea mays cDNA clone QAO8a04, mRNA sequence.    56   2e-13  3
dna update 2004. 1. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF407338_1(AF407338|pid:none) Lentinula edodes developmentally r...   182   2e-88
CP000081_526(CP000081|pid:none) Leishmania major chromosome 35, ...   191   8e-85
AM502253_524(AM502253|pid:none) Leishmania infantum chromosome 35.    189   7e-84
AM910993_119(AM910993|pid:none) Plasmodium knowlesi strain H chr...   161   1e-79
FM992692_447(FM992692|pid:none) Candida dubliniensis CD36 chromo...   167   4e-79
AE017346_325(AE017346|pid:none) Cryptococcus neoformans var. neo...   165   5e-78
AK066361_1(AK066361|pid:none) Oryza sativa Japonica Group cDNA c...   266   7e-70
AC146748_16(AC146748|pid:none) Medicago truncatula clone mth2-65...   264   4e-69
EU964619_1(EU964619|pid:none) Zea mays clone 280387 developmenta...   264   4e-69
EU236700_1(EU236700|pid:none) Pisum sativum developmentally regu...   263   6e-69
protein update 2009. 5.28
PSORT 

 psg: 0.45  gvh: 0.49  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for SFL427 is cyt
5' end seq. ID SFL427F
5' end seq. 
>SFL427F.Seq
CATCCTTTCTAAATCGAAATAACTTTAATTAATTAGGTTTTAGTTTATACAATATATAGG
GAGAAAATATATAATACAGAATGAGTCTTGTACAAAAAATCAAAGAGGTCGAGGATGAGA
TGGCTCGTACCCAAAAAAATAAAGCTACAAGCTTTCATTTAGGTGTATTGAAAGCAAAAT
TAGCAAAATATAAAAGAGAATTATTGTTAGGACCAAGTAAAGGTGCAGCAGCAGGTGCAG
GTGAAGGTTTCGATGTATCAAAAGCAGGTGATGCTCGTGTTGGTTTAATTGGTTTCCCAT
CAGTTGGTAAATCAACTTTATTAACTAAATTAACTGGTACCTCATCTGAAGTTGCCTCAT
ACGAATTCACAACTTTAACATGTATTCCTGGTGTAATTAATTATAAAGGTGCTAAAATTC
AATTATTAGATTTACCAGGTATTATTGAAGGTGCAAAAGATGGTAAAGGTAGAGGT----
------
Length of 5' end seq. 476
3' end seq. ID SFL427Z
3' end seq. 
>SFL427Z.Seq
----------TAGAGAGAGAGTTGGATGGTTTTGGTATTAGATTGAATAAACAACCCACC
ACCAATCACATTCAAAAAGAAGGAGAAGGGTGGTATTAACTTTTCACATACACCAAATGT
CAATCCAACCCAATTGGATAGTGAAACTGTGAAAGCAATCTGTGCAGAATATAAAATTCA
CAATGCAGATGTGATTTTACGTGGAAATTGTACAGTGGATGAATTCATCGATGTCATTGA
AGGCAATCGTATCTACGTTCCATGTATTTACGTTTTAAATAAGATCGATGCTATCTCTAT
CGAAGAGTTGGACCTTTTAGATAAAATTCCTCATTATGTTCCAATCTCTTCTCATTTGGA
ATGGAATTTAGATGCCCTCCTCGATAAAATTTGGGAATACTTAAAATTGATTAGAGTTTA
CACTAAACCAAAAGGTCTCATTCCAGATTACAATGAACCAGTCGTCATTAGAGGTGGTGA
AGAAGCTTCAATTGAAACTTTTTGTAATCATATTCATAATTCAATTATTAGACAATTTAG
ATATGCTTTGGTTTGGGGTTCTTCAGCTAAACATAACCCTCAACGTTGTGGTAAGGATCA
CGTTTTAGCAGATAAGATATGTTCAAATGTAAAAAATAAGATC
Length of 3' end seq. 633
Connected seq. ID SFL427P
Connected seq. 
>SFL427P.Seq
CATCCTTTCTAAATCGAAATAACTTTAATTAATTAGGTTTTAGTTTATACAATATATAGG
GAGAAAATATATAATACAGAATGAGTCTTGTACAAAAAATCAAAGAGGTCGAGGATGAGA
TGGCTCGTACCCAAAAAAATAAAGCTACAAGCTTTCATTTAGGTGTATTGAAAGCAAAAT
TAGCAAAATATAAAAGAGAATTATTGTTAGGACCAAGTAAAGGTGCAGCAGCAGGTGCAG
GTGAAGGTTTCGATGTATCAAAAGCAGGTGATGCTCGTGTTGGTTTAATTGGTTTCCCAT
CAGTTGGTAAATCAACTTTATTAACTAAATTAACTGGTACCTCATCTGAAGTTGCCTCAT
ACGAATTCACAACTTTAACATGTATTCCTGGTGTAATTAATTATAAAGGTGCTAAAATTC
AATTATTAGATTTACCAGGTATTATTGAAGGTGCAAAAGATGGTAAAGGTAGAGGT----
------TAGAGAGAGAGTTGGATGGTTTTGGTATTAGATTGAATAAACAACCCACCACCA
ATCACATTCAAAAAGAAGGAGAAGGGTGGTATTAACTTTTCACATACACCAAATGTCAAT
CCAACCCAATTGGATAGTGAAACTGTGAAAGCAATCTGTGCAGAATATAAAATTCACAAT
GCAGATGTGATTTTACGTGGAAATTGTACAGTGGATGAATTCATCGATGTCATTGAAGGC
AATCGTATCTACGTTCCATGTATTTACGTTTTAAATAAGATCGATGCTATCTCTATCGAA
GAGTTGGACCTTTTAGATAAAATTCCTCATTATGTTCCAATCTCTTCTCATTTGGAATGG
AATTTAGATGCCCTCCTCGATAAAATTTGGGAATACTTAAAATTGATTAGAGTTTACACT
AAACCAAAAGGTCTCATTCCAGATTACAATGAACCAGTCGTCATTAGAGGTGGTGAAGAA
GCTTCAATTGAAACTTTTTGTAATCATATTCATAATTCAATTATTAGACAATTTAGATAT
GCTTTGGTTTGGGGTTCTTCAGCTAAACATAACCCTCAACGTTGTGGTAAGGATCACGTT
TTAGCAGATAAGATATGTTCAAATGTAAAAAATAAGATC
Length of connected seq. 1109
Full length Seq ID -
Full length Seq. -
Length of full length seq. -