>AFI668 (AFI668Q) /CSM/AF/AFI6-C/AFI668Q.Seq.d/
ATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCAT
CAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGA
CCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGC
ATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGG
TAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGG
TAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCC
AAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGC
TGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAA
AATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAA
ATTAGCTATGCAAGAATTTATGATTTTACCAGXXXXXXXXXXCAAGATGCTATCAATGTT
GGTGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTA
AAACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCT
GCCTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGAC
GGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAA
TATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAA
TTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAA
AGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAA
ATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGT
GTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTT
GGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAA
TACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTA
ACTTTCCGTAAATTTTAATTTTACTCATAATA

LLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGA
STGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTP
NKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNK
LAMQEFMILP---

---QDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASEFKVENGYD
LDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVG
DDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETE
DTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFRKF*fys*

Frame A:
itlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssirc
inwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwyp
k*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**
isyariydft---

---QDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASEFKVENGYD
LDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVG
DDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETE
DTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFRKF*fys*

Frame B:
LLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGA
STGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTP
NKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNK
LAMQEFMILP---

---kmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlplnsklkmvti
ltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*qhqlifkllv
miyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswlaivqvklk
ipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vktitmlv*lsvnfnfthn

Frame C:
yfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvh
qlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpq
ikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin
*lcknl*fyq---

---rcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl*iqs*kwlrs
*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*insis*ysncw*
*ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgchg*psfr*n*r
ylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*kp*lcwfnfp*ilillii

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI668 (AFI668Q) /CSM/AF/AFI6-C/AFI668Q.Seq.d/                       2466   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1407   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1407   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1407   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1407   0.0
CHB226 (CHB226Q) /CSM/CH/CHB2-B/CHB226Q.Seq.d/                       1407   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1407   0.0
AFG692 (AFG692Q) /CSM/AF/AFG6-D/AFG692Q.Seq.d/                       1407   0.0
AFG292 (AFG292Q) /CSM/AF/AFG2-D/AFG292Q.Seq.d/                       1407   0.0
SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/                       1402   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X80474|X80474.1 N.frontalis enolase gene.                          64   9e-74  12
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   6e-71  11
CN587505|CN587505.1 USDA-FP_130580 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP098_G04, mRNA sequence.   101   7e-64  5
AY522934|AY522934.1 Artemia sp. SBH266677 2-phospho-D-glycerate hydrolase mRNA, partial cds.    70   1e-62  9
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-60  10
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-60  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   1e-60  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   1e-58  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   5e-52  5
CN756890|CN756890.1 ID0AAA19DG07RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA19DG07 5', mRNA sequence.   100   6e-50  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   461   0.0
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   336   e-154
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   325   e-151
BC071359_1(BC071359|pid:none) Danio rerio enolase 1, (alpha), mR...   315   e-147
EU306867_1(EU306867|pid:none) Cavia porcellus alpha-enolase (Eno...   316   e-146
BC059434_1(BC059434|pid:none) Danio rerio zgc:73056, mRNA (cDNA ...   315   e-146
BC092869_1(BC092869|pid:none) Danio rerio enolase 3, (beta, musc...   318   e-146
AY398342_1(AY398342|pid:none) Danio rerio clone RK122A2D02 enola...   318   e-146
BT044834_1(BT044834|pid:none) Salmo salar clone ssal-rgf-505-072...   313   e-146
BC054169_1(BC054169|pid:none) Xenopus laevis enolase 1, alpha, m...   310   e-146

 psg: 0.95  gvh: 0.47  alm: 0.33  top: 0.60  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for AFI668 is cyt

>AFI668F.Seq
ATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCAT
CAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGA
CCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGC
ATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGG
TAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGG
TAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCC
AAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGC
TGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAA
AATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAA
ATTAGCTATGCAAGAATTTATGATTTTACCAG----------

>AFI668Z.Seq
----------CAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCA
AAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGG
TTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGA
TCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGG
TGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGA
TCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGG
TGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATG
TAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGC
TCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGA
AGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGC
TCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTT
AGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATA

>AFI668P.Seq
ATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCAT
CAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGA
CCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGC
ATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGG
TAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGG
TAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCC
AAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGC
TGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAA
AATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAA
ATTAGCTATGCAAGAATTTATGATTTTACCAG----------CAAGATGCTATCAATGTT
GGTGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTA
AAACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCT
GCCTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGAC
GGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAA
TATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAA
TTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAA
AGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAA
ATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGT
GTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTT
GGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAA
TACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTA
ACTTTCCGTAAATTTTAATTTTACTCATAATA