>SFL243 (SFL243Q) /CSM/SF/SFL2-B/SFL243Q.Seq.d/
XXXXXXXXXXATCTAGTAAAAATATTACAGTCAATCCAATTAATTTAATTAAAAAAGTAG
AGATTAAAACTGGTGAATTTGGTATTACACCATACAATATACCAACACTCACTGAATCTT
ATAAAAGATATGTTACATTAGAAACTATTAGAGATTTAAAAGAAACTTCTTGTAGAGTTT
CTGATACAAAGGATTTAGGTGAAGATATAAATATAGCACAAGTACCATATGAATTGCCAG
ATGGTAATTTATTAGAGATTGGTTCAGATAGATTTCAAATTCCTGAATTATTATTTAATC
CAACACCATTGAATGAACAAATTTTAAATAACAATAATAATAATAGCAATGGTGAGAATA
CAACAACATCATCAGTTGTTCAACAACCACAATATCAAGGATTAGGAAGAATGATTATAG
AATCATTAGAGAAAAGTGATACAGATATTCGTAAAGAATTAATAAGTAATTTAATTATAA
CTGGTGGTAATACATTATTTGCAGGTTTCCAAGAAAGATTAGTTAGAGACATTTCTGAAA
TCTCTTATAATAAACCAAAAATCGTTGCAAATCAAACTGAAAAAAAGAATTCAGTTTGGA
TTGGTGGTTCTGTTCTTGGTTCATTAGNTACTGCTCCTC

---SSKNITVNPINLIKKVEIKTGEFGITPYNIPTLTESYKRYVTLETIRDLKETSCRVS
DTKDLGEDINIAQVPYELPDGNLLEIGSDRFQIPELLFNPTPLNEQILNNNNNNSNGENT
TTSSVVQQPQYQGLGRMIIESLEKSDTDIRKELISNLIITGGNTLFAGFQERLVRDISEI
SYNKPKIVANQTEKKNSVWIGGSVLGSLXTAP

Frame A:
---SSKNITVNPINLIKKVEIKTGEFGITPYNIPTLTESYKRYVTLETIRDLKETSCRVS
DTKDLGEDINIAQVPYELPDGNLLEIGSDRFQIPELLFNPTPLNEQILNNNNNNSNGENT
TTSSVVQQPQYQGLGRMIIESLEKSDTDIRKELISNLIITGGNTLFAGFQERLVRDISEI
SYNKPKIVANQTEKKNSVWIGGSVLGSLXTAP


Frame B:
---lvkilqsiqli*lkk*rlklvnlvlhhtiyqhslnlikdmlh*kllei*kkllvefl
iqri*vki*i*hkyhmncqmviy*rlvqidfkflnyyliqhh*mnkf*itiiiiamvriq
qhhqlfnnhnikd*ee*l*nh*rkviqifvkn**vi*l*lvvihylqvskkd*letflks
liinqkslqiklkkriqfglvvlflvh*xlll


Frame C:
---**kyysqsn*fn*ksrd*nw*iwyytiqytnth*il*kicyirny*rfkrnfl*sf*
ykgfr*rykyststi*iarw*firdwfr*isns*iii*sntie*tnfk*q****qw*eyn
niiscstttisrirkndyriirek*yrys*rink*fnynww*yiicrfprkis*rhf*nl
l**tknrcksn*kkefsldwwfcswfixycs

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL243 (SFL243Q) /CSM/SF/SFL2-B/SFL243Q.Seq.d/                       1199   0.0
CFH524 (CFH524Q) /CSM/CF/CFH5-A/CFH524Q.Seq.d/                       1160   0.0
SFC783 (SFC783Q) /CSM/SF/SFC7-D/SFC783Q.Seq.d/                       1144   0.0
SFF737 (SFF737Q) /CSM/SF/SFF7-B/SFF737Q.Seq.d/                       1138   0.0
AFG296 (AFG296Q) /CSM/AF/AFG2-D/AFG296Q.Seq.d/                       1138   0.0
AFM734 (AFM734Q) /CSM/AF/AFM7-B/AFM734Q.Seq.d/                       1132   0.0
SFB730 (SFB730Q) /CSM/SF/SFB7-B/SFB730Q.Seq.d/                        955   0.0
CFK273 (CFK273Q) /CSM/CF/CFK2-D/CFK273Q.Seq.d/                        878   0.0
AFL224 (AFL224Q) /CSM/AF/AFL2-A/AFL224Q.Seq.d/                        769   0.0
AFA162 (AFA162Q) /CSM/AF/AFA1-C/AFA162Q.Seq.d/                        654   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    46   9e-09  6
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    44   6e-08  5
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    42   8e-08  6
AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.    48   5e-07  4
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    46   1e-06  7
AE014850|AE014850.1 Plasmodium falciparum 3D7 chromosome 12, section 7 of 9 of the complete sequence.    38   1e-06  11
AC116032|AC116032.2 Dictyostelium discoideum chromosome 2 map 4158743-4189373 strain AX4, complete sequence.    32   2e-06  9
AE014838|AE014838.1 Plasmodium falciparum 3D7 chromosome 11 section 3 of 8 of the complete sequence.    38   2e-06  12
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    32   3e-06  16
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           42   4e-06  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT038602_1(BT038602|pid:none) Zea mays full-length cDNA clone ZM...   139   7e-32
(Q84M92) RecName: Full=Actin-related protein 4;  &AK228098_1(AK2...   138   1e-31
AY087788_1(AY087788|pid:none) Arabidopsis thaliana clone 38419 m...   137   2e-31
AC152123_6(AC152123|pid:none) Genomic sequence for broccoli [Bra...   136   4e-31
CR954204_278(CR954204|pid:none) Ostreococcus tauri strain OTTH05...   115   8e-25
CP001330_208(CP001330|pid:none) Micromonas sp. RCC299 chromosome...   115   8e-25
DQ980427_1(DQ980427|pid:none) Peridinium limbatum clone plactin2...   107   4e-22
EU742777_1(EU742777|pid:none) Amphidinium carterae strain CCMP13...   107   4e-22
EU742685_1(EU742685|pid:none) Amphidinium carterae strain CCMP13...   107   4e-22
DQ980429_1(DQ980429|pid:none) Peridinium limbatum clone plactin2...   105   8e-22

 psg: 0.79  gvh: 0.25  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SFL243 is nuc

>SFL243Z.Seq
----------ATCTAGTAAAAATATTACAGTCAATCCAATTAATTTAATTAAAAAAGTAG
AGATTAAAACTGGTGAATTTGGTATTACACCATACAATATACCAACACTCACTGAATCTT
ATAAAAGATATGTTACATTAGAAACTATTAGAGATTTAAAAGAAACTTCTTGTAGAGTTT
CTGATACAAAGGATTTAGGTGAAGATATAAATATAGCACAAGTACCATATGAATTGCCAG
ATGGTAATTTATTAGAGATTGGTTCAGATAGATTTCAAATTCCTGAATTATTATTTAATC
CAACACCATTGAATGAACAAATTTTAAATAACAATAATAATAATAGCAATGGTGAGAATA
CAACAACATCATCAGTTGTTCAACAACCACAATATCAAGGATTAGGAAGAATGATTATAG
AATCATTAGAGAAAAGTGATACAGATATTCGTAAAGAATTAATAAGTAATTTAATTATAA
CTGGTGGTAATACATTATTTGCAGGTTTCCAAGAAAGATTAGTTAGAGACATTTCTGAAA
TCTCTTATAATAAACCAAAAATCGTTGCAAATCAAACTGAAAAAAAGAATTCAGTTTGGA
TTGGTGGTTCTGTTCTTGGTTCATTAGNTACTGCTCCTC