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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL233
Library SF
(Link to library)
Clone ID SFL233
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID SFL233P
(Link to Original site)
Representative DNA sequence 
>SFL233 (SFL233Q) /CSM/SF/SFL2-B/SFL233Q.Seq.d/
ATCGCTTCCGACTTCGGTTAATAACGAAAAAGATTTTCGCCTTTTACTAAAATACTTCCG
TGAACCGAAGTCATAAAGGCAGCTTTCACTATTTTTTTGAATAGGTCTTCCTATTCCAAA
AACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAAGATGTTCAAGCTTTAG
TTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGACGATGCTCCACGTG
CTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTC
AAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAAT
ACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATA
CTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGTTCTCTTAACCGAAGCTC
XXXXXXXXXXGAACAAANAATTAACTGCTTTAGCNCCNTCAACCATGAAAATTAAAATCN
TTGNTCCCCCANAACGTAAANACNCTGTNTGGATTGGTGGATNTATTTTAGNTTCNCTNT
CAACNTTCCNACAAATGTGGATNTCNAAANAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
rfrlrlitkkifafy*ntsvnrshkgsfhyffe*vflfqkqin*ik*KIKMDGEDVQALV
IDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKY
PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEA---

---NKXLTALAPSTMKIKIXXPPXRKXXVWIGGXILXSLSTFXQMWXSKX
Translated Amino Acid sequence (All Frames) 
Frame A:
iasdfg**rkrfspftkilp*tevikaaftiflnrssysknk*iklnkk*kwmvkmfkl*
llitvlvcvkpvllvtmlhvlfshqllvvqdtlvlwlvwvkkthm*vmkpnqrevs*psn
tqlntvslptgmiwkksgiilstmnsvlhqkntqfs*pkl---

---eqxincfsxxnhen*nxxspxt*xxcxdwwxyfxfxxnxptnvdxxx

Frame B:
slptsvnnekdfrlllkyfrepks*rqlslff*iglpipktnkln*iknkngw*rcssfs
y**rfwyv*srfcw*rcstccfpincwsskthwcygwygskrlicr**spikerylnpqi
pn*tryryqlg*ygknlasyflq*tpcctrrtpsslnrs---

---NKXLTALAPSTMKIKIXXPPXRKXXVWIGGXILXSLSTFXQMWXSKX

Frame C:
rfrlrlitkkifafy*ntsvnrshkgsfhyffe*vflfqkqin*ik*KIKMDGEDVQALV
IDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKY
PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEA---

---txn*ll*xxqp*klkslxpxnvxtlxglvdxf*xxxqxsxkcgxxx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM527 (VFM527Q) /CSM/VF/VFM5-B/VFM527Q.Seq.d/                        648   0.0
VFL462 (VFL462Q) /CSM/VF/VFL4-C/VFL462Q.Seq.d/                        648   0.0
VFL353 (VFL353Q) /CSM/VF/VFL3-C/VFL353Q.Seq.d/                        648   0.0
VFL311 (VFL311Q) /CSM/VF/VFL3-A/VFL311Q.Seq.d/                        648   0.0
VFK419 (VFK419Q) /CSM/VF/VFK4-A/VFK419Q.Seq.d/                        648   0.0
VFK366 (VFK366Q) /CSM/VF/VFK3-C/VFK366Q.Seq.d/                        648   0.0
VFK110 (VFK110Q) /CSM/VF/VFK1-A/VFK110Q.Seq.d/                        648   0.0
VFJ294 (VFJ294Q) /CSM/VF/VFJ2-D/VFJ294Q.Seq.d/                        648   0.0
VFI680 (VFI680Q) /CSM/VF/VFI6-D/VFI680Q.Seq.d/                        648   0.0
SLH494 (SLH494Q) /CSM/SL/SLH4-D/SLH494Q.Seq.d/                        648   0.0
own update 2004. 7.23
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   573   0.0    5
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.         648   0.0    2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   648   0.0    3
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.       589   0.0    2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   585   0.0    5
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   529   0.0    6
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   640   e-180  1
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   517   e-170  4
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   502   e-165  2
X03282|X03282.1 dictyostelium discoideum gene for actin A12.      492   e-165  2
dna update 2004. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A61043(A61043)actin CA15 - sea squirt (Styela clava) (fragments)      253   2e-66
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   230   2e-59
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   230   2e-59
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   229   6e-59
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   228   8e-59
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        226   5e-58
AB111103_1(AB111103|pid:none) Candida boidinii ACT1 gene for act...   224   2e-57
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   224   2e-57
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   224   2e-57
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   223   3e-57
protein update 2009. 5.28
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SFL233 is cyt
5' end seq. ID SFL233F
5' end seq. 
>SFL233F.Seq
ATCGCTTCCGACTTCGGTTAATAACGAAAAAGATTTTCGCCTTTTACTAAAATACTTCCG
TGAACCGAAGTCATAAAGGCAGCTTTCACTATTTTTTTGAATAGGTCTTCCTATTCCAAA
AACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAAGATGTTCAAGCTTTAG
TTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGACGATGCTCCACGTG
CTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTC
AAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAAT
ACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATA
CTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGTTCTCTTAACCGAAGCTC
----------
Length of 5' end seq. 480
3' end seq. ID SFL233Z
3' end seq. 
>SFL233Z.Seq
----------GAACAAANAATTAACTGCTTTAGCNCCNTCAACCATGAAAATTAAAATCN
TTGNTCCCCCANAACGTAAANACNCTGTNTGGATTGGTGGATNTATTTTAGNTTCNCTNT
CAACNTTCCNACAAATGTGGATNTCNAAANAA
Length of 3' end seq. 142
Connected seq. ID SFL233P
Connected seq. 
>SFL233P.Seq
ATCGCTTCCGACTTCGGTTAATAACGAAAAAGATTTTCGCCTTTTACTAAAATACTTCCG
TGAACCGAAGTCATAAAGGCAGCTTTCACTATTTTTTTGAATAGGTCTTCCTATTCCAAA
AACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAAGATGTTCAAGCTTTAG
TTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGACGATGCTCCACGTG
CTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTC
AAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAAT
ACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATA
CTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGTTCTCTTAACCGAAGCTC
----------GAACAAANAATTAACTGCTTTAGCNCCNTCAACCATGAAAATTAAAATCN
TTGNTCCCCCANAACGTAAANACNCTGTNTGGATTGGTGGATNTATTTTAGNTTCNCTNT
CAACNTTCCNACAAATGTGGATNTCNAAANAA
Length of connected seq. 622
Full length Seq ID -
Full length Seq. -
Length of full length seq. -