>SFL222 (SFL222Q) /CSM/SF/SFL2-A/SFL222Q.Seq.d/
TTACCTCATGTACTGAGGCAATTGAAATGATCTCAAAACTTCCACAAGAAGTATTTGAAA
AAAGTACAAGAACAGCAACAAAGAAATTATTAGCATCAATTGGTTTAGTGACAATTGGTA
CATATTTAGTATATGCACTTCCATGGTATTTGTTACCAATTGGATGGATATTTATGGGTA
CAGCATGTTGTGGATTATTCTCTGTTGGGTATGCATGTGGTAATGATTTATTTTTCAAGA
ATAAATTTGTTAACTATGTAGTTGGAACAATTTGTATGTTACCATTAATGTATCCATTAG
CCTATTGGAAGAAAAGAATTGAAAAGAAAGCAACAGCAAGTCGTGATTTCGTTCGTGAGA
TTGCAAGTGGTAATTTTTGGTTCCTCAGTTCATTACTTCAATGGTTATCATTAAATTTCA
GTTTCAAATTCTCCAAATCAATGATGGTCTCAGTCTCATTCCTTTATGTATTCATCGCAT
TATTCTTCCCAATTATGACCTACAGTATGGGCATTTGGGGTTTATTCAAGTACTATTTGA
TCCCATTGTTTGTGTACCACTTTTGGATGTCAACATTCCTCAAGGCTTCATCTCTCTCAA
XXXXXXXXXXTCCAACATAAATATTTCATTCCATTGGCATTCAGTTTGGGTTGGTTCTTA
CCAATGTTCATTTGTGGTTTTGGTTGGAATGATTGGAAAGGTGGATTTTTCATCGCTGGT
GTTGCCTCCAAAGTACTCATGATGCAATGTACCTTTTGTATCAACTCTTTGGCTCATTAT
CTTGGTGAAGCAACTTACACCGACCAAAGATCTCCACGTGACTCTTTTATCACATCTTTG
GTCACTTTTGGTGAAGGTTATCATAACTTTCATCATGAATTCCCATATGATTACAGAAAT
GGTATTCATATGTCTGCTTACGATCCAGGTAAGTGGTTAATCAATTTCCTTAGTTGGTTT
GGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTAAAATTCAA
ATGCAAGAGAAAACCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAACCATTGGAT
CAATTACCATCATTCACTCGTGCTCAAGTTAAAGAGAAATGTCAAAAAGAAAAGAAACAA
CTCTTAATCATTGAAGATGTTATCTATGATATGTCTGAATTTGCTGAAAAACATCCAGGT
GGTAAACAATACATTGACGGTTATATTGGTAAAGATGCAACTAAAGCTTTTAATGGTTTA
GTTTAAATCATTCATTTGCTGCTAGAAATATTGGATCTTTATAGAG

TSCTEAIEMISKLPQEVFEKSTRTATKKLLASIGLVTIGTYLVYALPWYLLPIGWIFMGT
ACCGLFSVGYACGNDLFFKNKFVNYVVGTICMLPLMYPLAYWKKRIEKKATASRDFVREI
ASGNFWFLSSLLQWLSLNFSFKFSKSMMVSVSFLYVFIALFFPIMTYSMGIWGLFKYYLI
PLFVYHFWMSTFLKASSLS---

---QHKYFIPLAFSLGWFLPMFICGFGWNDWKGGFFIAGVASKVLMMQCTFCINSLAHYL
GEATYTDQRSPRDSFITSLVTFGEGYHNFHHEFPYDYRNGIHMSAYDPGKWLINFLSWFG
FAYELKRFPSELFAKGKIQMQEKTIKKLRDQLYWGKPLDQLPSFTRAQVKEKCQKEKKQL
LIIEDVIYDMSEFAEKHPGGKQYIDGYIGKDATKAFNGLV*iihllleildlyr

Frame A:
lphvlrqlk*sqnfhkkylkkvqeqqqrny*hqlv**qlvhi*ymhfhgicyqldgylwv
qhvvdysllgmhvvmiyfsrinlltm*leqfvcyh*cih*pigrkelkrkqqqvvisfvr
lqvvifgssvhyfngyh*isvsnspnq*wsqshsfmysshyssql*ptvwafgvyssti*
shclcttfgcqhssrlhlsq---

---sninisfhwhsvwvgsyqcsfvvlvgmigkvdfsslvlppkys*cnvpfvstlwlii
lvkqltptkdlhvtllshlwsllvkviitfimnshmitemvficlltiqvsg*sislvgl
vspmn*rdsqvnysqkvkfkckrkpsknyvinsigvnhwinyhhslvlklkrnvkkkrnn
s*slkmlsmiclnllkniqvvnntltvilvkmqlkllmv*fksficc*kywifie

Frame B:
ylmy*gn*ndlktstrsi*kkyknsnkeiisinwfsdnwyifsictsmvfvtnwmdiygy
smlwiilcwvcmw**fifqe*ic*lcswnnlyvtinvsislleekn*kesnsks*frs*d
ckw*flvpqfitsmviikfqfqilqindglsliplcihriilpnydlqyghlgfiqvlfd
pivcvplldvnipqgfisl---

---pt*ifhsigiqfglvltnvhlwfwle*lerwifhrwcclqsthdamyllyqlfgsls
w*snlhrpkist*lfyhifghfw*rls*lss*ipi*lqkwysyvclrsr*vvnqfp*lvw
frl*ikeipk*iirkr*nsnarenhqkit*stllg*tigsitiihscss*remskrkett
lnh*rcyl*yv*ic*ktsrw*tih*rlyw*rcn*sf*wfslnhsfaarnigsl*

Frame C:
TSCTEAIEMISKLPQEVFEKSTRTATKKLLASIGLVTIGTYLVYALPWYLLPIGWIFMGT
ACCGLFSVGYACGNDLFFKNKFVNYVVGTICMLPLMYPLAYWKKRIEKKATASRDFVREI
ASGNFWFLSSLLQWLSLNFSFKFSKSMMVSVSFLYVFIALFFPIMTYSMGIWGLFKYYLI
PLFVYHFWMSTFLKASSLS---

---QHKYFIPLAFSLGWFLPMFICGFGWNDWKGGFFIAGVASKVLMMQCTFCINSLAHYL
GEATYTDQRSPRDSFITSLVTFGEGYHNFHHEFPYDYRNGIHMSAYDPGKWLINFLSWFG
FAYELKRFPSELFAKGKIQMQEKTIKKLRDQLYWGKPLDQLPSFTRAQVKEKCQKEKKQL
LIIEDVIYDMSEFAEKHPGGKQYIDGYIGKDATKAFNGLV*iihllleildlyr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL222 (SFL222Q) /CSM/SF/SFL2-A/SFL222Q.Seq.d/                       2529   0.0
CHO352 (CHO352Q) /CSM/CH/CHO3-C/CHO352Q.Seq.d/                       1366   0.0
VHM540 (VHM540Q) /CSM/VH/VHM5-B/VHM540Q.Seq.d/                       1358   0.0
CHN780 (CHN780Q) /CSM/CH/CHN7-D/CHN780Q.Seq.d/                       1358   0.0
VHL116 (VHL116Q) /CSM/VH/VHL1-A/VHL116Q.Seq.d/                       1342   0.0
VHO360 (VHO360Q) /CSM/VH/VHO3-C/VHO360Q.Seq.d/                       1332   0.0
VHD238 (VHD238Q) /CSM/VH/VHD2-B/VHD238Q.Seq.d/                       1332   0.0
VHC619 (VHC619Q) /CSM/VH/VHC6-A/VHC619Q.Seq.d/                       1332   0.0
VHB877 (VHB877Q) /CSM/VH/VHB8-D/VHB877Q.Seq.d/                       1332   0.0
VHA359 (VHA359Q) /CSM/VH/VHA3-C/VHA359Q.Seq.d/                       1332   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    74   6e-09  1
AR016873|AR016873.1 Sequence 1 from patent US 5777201.             56   5e-08  3
AL033501|AL033501.1 C.albicans cosmid Ca41C10.                     70   9e-08  1
Z72577|Z72577.1 S.cerevisiae chromosome VII reading frame ORF YGL055w.    56   3e-07  3
AQ874658|AQ874658.1 V112D8 mTn-3xHA/lacZ Insertion Library, strain Y2278 Saccharomyces cerevisiae genomic 5', genomic survey sequence.    56   4e-07  2
J05676|J05676.1 Saccharomyces cerevisiae delta-9 fatty acid desaturase (OLE1) gene, complete cds.    56   3e-04  2
AL396412|AL396412.1 T3 end of clone AR0AA030B05 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    56   0.001  1
AL117199|AL117199.1 Caenorhabditis elegans YAC Y43F11A.            42   0.003  2
BM027442|BM027442.1 GIT0000801 Root-induced cDNA library from Glomus intraradices Glomus intraradices cDNA, mRNA sequence.    54   0.005  1
AB024576|AB024576.1 Pichia angusta H-OLE1 gene for delta9-fatty acid desaturase, complete cds.    54   0.005  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY995173_1(AY995173|pid:none) Amylomyces rouxii delta-9 desatura...   234   5e-60
AB196774_1(AB196774|pid:none) Mortierella alpina gene for omega9...   226   1e-57
AF085500_1(AF085500|pid:none) Mortierella alpina delta nine desa...   219   2e-55
Y18553_1(Y18553|pid:none) Mortierella alpina ole1 gene for stear...   218   3e-55
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   216   2e-54
AB195980_1(AB195980|pid:none) Mortierella alpina gene for omega9...   214   5e-54
AP007151_392(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   211   4e-53
AM920435_140(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   208   4e-52
CR382123_238(CR382123|pid:none) Kluyveromyces lactis strain NRRL...   207   5e-52
AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   206   1e-51

 psg: 0.75  gvh: 0.50  alm: 0.24  top: 0.47  tms: 0.33  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: endoplasmic reticulum
	 28.0 %: plasma membrane
	 16.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SFL222 is end

>SFL222F.Seq
TTACCTCATGTACTGAGGCAATTGAAATGATCTCAAAACTTCCACAAGAAGTATTTGAAA
AAAGTACAAGAACAGCAACAAAGAAATTATTAGCATCAATTGGTTTAGTGACAATTGGTA
CATATTTAGTATATGCACTTCCATGGTATTTGTTACCAATTGGATGGATATTTATGGGTA
CAGCATGTTGTGGATTATTCTCTGTTGGGTATGCATGTGGTAATGATTTATTTTTCAAGA
ATAAATTTGTTAACTATGTAGTTGGAACAATTTGTATGTTACCATTAATGTATCCATTAG
CCTATTGGAAGAAAAGAATTGAAAAGAAAGCAACAGCAAGTCGTGATTTCGTTCGTGAGA
TTGCAAGTGGTAATTTTTGGTTCCTCAGTTCATTACTTCAATGGTTATCATTAAATTTCA
GTTTCAAATTCTCCAAATCAATGATGGTCTCAGTCTCATTCCTTTATGTATTCATCGCAT
TATTCTTCCCAATTATGACCTACAGTATGGGCATTTGGGGTTTATTCAAGTACTATTTGA
TCCCATTGTTTGTGTACCACTTTTGGATGTCAACATTCCTCAAGGCTTCATCTCTCTCAA
----------

>SFL222Z.Seq
----------TCCAACATAAATATTTCATTCCATTGGCATTCAGTTTGGGTTGGTTCTTA
CCAATGTTCATTTGTGGTTTTGGTTGGAATGATTGGAAAGGTGGATTTTTCATCGCTGGT
GTTGCCTCCAAAGTACTCATGATGCAATGTACCTTTTGTATCAACTCTTTGGCTCATTAT
CTTGGTGAAGCAACTTACACCGACCAAAGATCTCCACGTGACTCTTTTATCACATCTTTG
GTCACTTTTGGTGAAGGTTATCATAACTTTCATCATGAATTCCCATATGATTACAGAAAT
GGTATTCATATGTCTGCTTACGATCCAGGTAAGTGGTTAATCAATTTCCTTAGTTGGTTT
GGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTAAAATTCAA
ATGCAAGAGAAAACCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAACCATTGGAT
CAATTACCATCATTCACTCGTGCTCAAGTTAAAGAGAAATGTCAAAAAGAAAAGAAACAA
CTCTTAATCATTGAAGATGTTATCTATGATATGTCTGAATTTGCTGAAAAACATCCAGGT
GGTAAACAATACATTGACGGTTATATTGGTAAAGATGCAACTAAAGCTTTTAATGGTTTA
GTTTAAATCATTCATTTGCTGCTAGAAATATTGGATCTTTATAGAG

>SFL222P.Seq
TTACCTCATGTACTGAGGCAATTGAAATGATCTCAAAACTTCCACAAGAAGTATTTGAAA
AAAGTACAAGAACAGCAACAAAGAAATTATTAGCATCAATTGGTTTAGTGACAATTGGTA
CATATTTAGTATATGCACTTCCATGGTATTTGTTACCAATTGGATGGATATTTATGGGTA
CAGCATGTTGTGGATTATTCTCTGTTGGGTATGCATGTGGTAATGATTTATTTTTCAAGA
ATAAATTTGTTAACTATGTAGTTGGAACAATTTGTATGTTACCATTAATGTATCCATTAG
CCTATTGGAAGAAAAGAATTGAAAAGAAAGCAACAGCAAGTCGTGATTTCGTTCGTGAGA
TTGCAAGTGGTAATTTTTGGTTCCTCAGTTCATTACTTCAATGGTTATCATTAAATTTCA
GTTTCAAATTCTCCAAATCAATGATGGTCTCAGTCTCATTCCTTTATGTATTCATCGCAT
TATTCTTCCCAATTATGACCTACAGTATGGGCATTTGGGGTTTATTCAAGTACTATTTGA
TCCCATTGTTTGTGTACCACTTTTGGATGTCAACATTCCTCAAGGCTTCATCTCTCTCAA
----------TCCAACATAAATATTTCATTCCATTGGCATTCAGTTTGGGTTGGTTCTTA
CCAATGTTCATTTGTGGTTTTGGTTGGAATGATTGGAAAGGTGGATTTTTCATCGCTGGT
GTTGCCTCCAAAGTACTCATGATGCAATGTACCTTTTGTATCAACTCTTTGGCTCATTAT
CTTGGTGAAGCAACTTACACCGACCAAAGATCTCCACGTGACTCTTTTATCACATCTTTG
GTCACTTTTGGTGAAGGTTATCATAACTTTCATCATGAATTCCCATATGATTACAGAAAT
GGTATTCATATGTCTGCTTACGATCCAGGTAAGTGGTTAATCAATTTCCTTAGTTGGTTT
GGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTAAAATTCAA
ATGCAAGAGAAAACCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAACCATTGGAT
CAATTACCATCATTCACTCGTGCTCAAGTTAAAGAGAAATGTCAAAAAGAAAAGAAACAA
CTCTTAATCATTGAAGATGTTATCTATGATATGTCTGAATTTGCTGAAAAACATCCAGGT
GGTAAACAATACATTGACGGTTATATTGGTAAAGATGCAACTAAAGCTTTTAATGGTTTA
GTTTAAATCATTCATTTGCTGCTAGAAATATTGGATCTTTATAGAG