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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL216
Library SF
(Link to library)
Clone ID SFL216
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16268-1
Original site URL
Representative seq. ID SFL216P
(Link to Original site)
Representative DNA sequence 
>SFL216 (SFL216Q) /CSM/SF/SFL2-A/SFL216Q.Seq.d/
ATTTTCATTTTTTTTATCGCCTCACTTTTTATTTTTGTATTATATTCTCTCTTTATTTCT
TTTCTATATATAGATAAATTTTTTTTAGAAAAACTAAAAAAAAAACCTCAAACAATTAAT
ACAAAATGGTAAACATTGGTGGAACTCAAGAAAAATGTACTGCATGTGCTAAAACTGTAT
ACTTAACAGAAAAGATTGTAGTTGAAGATAAAGAAGATAAGAAAACTTTCCACAAATTAT
GTTTAAAATGTACTCACTGTAAGCTTACATTAAGTCTTGGTAACTATGCATCATTAAATG
GNGTATTCTATTGTAAACCACATTTCAAACAATTATTCGCCACCAAAGGTAACTACGATG
AAGGTTTCGGTAAATCAAAACATTCTGAAAAATGGACACCACAAGCCACCCCAACTGGTA
CTTCTTCATTCATTCCAGTTGAAGAATCAAAATCATCAGAAAAGAAAGAAACACCAACAA
CAATTTCATCAAAATTCTCAGGATCAACTGAAAAATGTAATCTCTGTAATAAGACAGTAT
ATTTAACAGAAAAGATTGTTGTTGAAGATAAAGAAGATAAGAAAGTTTTACATAAACAAT
GTTTGAAATGTACACATTGGAGNGGTGGATTAAATCTTGGTACCTATGCATCAATGAAAA
GGNGTATTCTATTGGAAACCACATTTCAAACAATTATTCGCCCCCAANGGTACCTNTGAT
GNAAGGTTTXXXXXXXXXXTGCCAAGAAATTCTCAACTGGTAGTTCAGAGAAATGTCACG
ATTGTCAAAAATCAGTTTACCTCACAGAGAAAGTCGTACTTGAGGAATTGGAAAACAAGA
GAATCTTCCACAAGGCTTGTTTGAAATGTAGTAAATGTTCAGTCATTCTCACCTTGGGTA
CTTTGGTTCAATTGGATGGTGTTATCTATTGTAAACCACACTTTAAAGAACTCTATGCCA
CTCAAGGTAATTTAGATGGTGGTTTCGGTAAACCAAAACACTCTGAAAAATGGGAAAATA
ACCCATTCCCATATTTAGAAAAGGATAGCAGTTATGTACCATCCGTTGAAAAGGTTGAAT
CTCAAATGAATAATGCTTCATCTGAATCTGATGTTGATAGATTTAAAAATATTAACAATG
ATGATTCTGATAAACCAAGAAGACAACAACAACCAGTTTCTCAACCAGAAGAAGAACAAC
AACAACAAGAAGAAGAAAAAGTTGAAGAACCAATCTCTGAAGAAGAAGAAAAAGTTGAAG
AAAAAGTTGAAGAACCAGTCGCTGAACAAGAAGAAGAAAAGGTTGAGGAACCAGTTGTTG
CTGAAGAAGAAAAAGTTGAAGAACCAGTTACTGAAGAAGAAAAAGTTGAAGAACCAGTCG
TTGCCTCTAAGAACCAGTNAAGAAGAAACAAAAGCNAAGAAGAAAACT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fsfflsphflflyyilslflfyi*inff*kn*kknlkqliqngkhwwnsrkmycmc*nci
lnrkdcs*r*rr*enfpqimfkmysl*ayiksw*lciikwxill*ttfqtiirhqr*lr*
rfr*iktf*kmdttshpnwyffihss*rikiirkerntnnnfikilrin*km*sl**dsi
fnrkdcc*r*rr*esft*TMFEMYTLEXWIKSWYLCINEKXYSIGNHISNNYSPPXVPXM
XG---

---AKKFSTGSSEKCHDCQKSVYLTEKVVLEELENKRIFHKACLKCSKCSVILTLGTLVQ
LDGVIYCKPHFKELYATQGNLDGGFGKPKHSEKWENNPFPYLEKDSSYVPSVEKVESQMN
NASSESDVDRFKNINNDDSDKPRRQQQPVSQPEEEQQQQEEEKVEEPISEEEEKVEEKVE
EPVAEQEEEKVEEPVVAEEEKVEEPVTEEEKVEEPVVASKNQXRRNKSXEEN
Translated Amino Acid sequence (All Frames) 
Frame A:
ififfiaslfifvlyslfisflyidkffleklkkkpqtintkw*tlvelkknvlhvlkly
t*qkrl*lkikkirklstnyv*nvltvslh*vlvtmhh*mxysivnhisnnysppkvttm
kvsvnqnilknghhkppqlvllhsfqlknqnhqkrkkhqqqfhqnsqdqlknvisvirqy
i*qkrlllkikkirkfyinnv*nvhigxvd*ilvpmhq*kgvfywkphfkqlfapxgtxd
xrf---

---cqeilnw*fremsrlskislphresrt*gigkqenlpqglfem**mfshshlgyfgs
igwcyll*ttl*rtlchsr*frwwfr*tktl*kmgk*pipifrkg*qlctir*kg*isne
*cfi*i*c**i*ky*q**f**tkkttttsfstrrrttttrrrks*rtnl*rrrks*rks*
rtsr*trrrkg*gtscc*rrks*rtsy*rrks*rtsrcl*epvkkkqkxrrk

Frame B:
fsfflsphflflyyilslflfyi*inff*kn*kknlkqliqngkhwwnsrkmycmc*nci
lnrkdcs*r*rr*enfpqimfkmysl*ayiksw*lciikwxill*ttfqtiirhqr*lr*
rfr*iktf*kmdttshpnwyffihss*rikiirkerntnnnfikilrin*km*sl**dsi
fnrkdcc*r*rr*esft*TMFEMYTLEXWIKSWYLCINEKXYSIGNHISNNYSPPXVPXM
XG---

---AKKFSTGSSEKCHDCQKSVYLTEKVVLEELENKRIFHKACLKCSKCSVILTLGTLVQ
LDGVIYCKPHFKELYATQGNLDGGFGKPKHSEKWENNPFPYLEKDSSYVPSVEKVESQMN
NASSESDVDRFKNINNDDSDKPRRQQQPVSQPEEEQQQQEEEKVEEPISEEEEKVEEKVE
EPVAEQEEEKVEEPVVAEEEKVEEPVTEEEKVEEPVVASKNQXRRNKSXEEN

Frame C:
fhffyrltfyfciifslyffsiyr*iffrktkkktsnn*ykmvniggtqekctacaktvy
ltekivvedkedkktfhklclkcthckltlslgnyaslngvfyckphfkqlfatkgnyde
gfgkskhsekwtpqatptgtssfipveeskssekketpttisskfsgstekcnlcnktvy
ltekivvedkedkkvlhkqclkcthwxgglnlgtyasmkrxillettfqtiirpqxyl*x
kv---

---prnsqlvvqrnvtivknqftsqrksylrnwktresstrlv*nvvnvqsfspwvlwfn
wmvlsivnhtlknsmplkvi*mvvsvnqntlkngkithshi*kriavmyhplkrlnlk*i
mlhlnlmlidlkiltmmilinqednnnqflnqkknnnnkkkkklknqslkkkkklkkklk
nqslnkkkkrlrnqlllkkkklknqllkkkklknqslplrtsxeetkakkkt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL216 (SFL216Q) /CSM/SF/SFL2-A/SFL216Q.Seq.d/                       1996   0.0
SFD757 (SFD757Q) /CSM/SF/SFD7-C/SFD757Q.Seq.d/                       1158   0.0
SFK508 (SFK508Q) /CSM/SF/SFK5-A/SFK508Q.Seq.d/                       1144   0.0
AFI579 (AFI579Q) /CSM/AF/AFI5-D/AFI579Q.Seq.d/                       1144   0.0
SFH103 (SFH103Q) /CSM/SF/SFH1-A/SFH103Q.Seq.d/                       1142   0.0
AFL556 (AFL556Q) /CSM/AF/AFL5-C/AFL556Q.Seq.d/                       1138   0.0
AFL719 (AFL719Q) /CSM/AF/AFL7-A/AFL719Q.Seq.d/                       1136   0.0
AFM428 (AFM428Q) /CSM/AF/AFM4-B/AFM428Q.Seq.d/                       1134   0.0
VFK172 (VFK172Q) /CSM/VF/VFK1-C/VFK172Q.Seq.d/                       1132   0.0
AFN432 (AFN432Q) /CSM/AF/AFN4-B/AFN432Q.Seq.d/                       1132   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BE858201|BE858201.1 7g19d02.x1 NCI_CGAP_Brn23 Homo sapiens cDNA clone IMAGE:3306915 3'TR:Q9Y659 TR:Q9UIY0 ;, mRNA sequence.    52   0.022  1
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   0.046  16
AC093893|AC093893.2 Homo sapiens BAC clone RP11-707F2 from 4, complete sequence.    34   0.054  11
BI504534|BI504534.1 BB170004B20D05 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170004B20D05 5', mRNA sequence.    38   0.068  2
AL929351|AL929351.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 1/4.    34   0.090  14
AC124624|AC124624.3 Mus musculus clone RP23-417K9, WORKING DRAFT SEQUENCE, 5 unordered pieces.    44   0.10   10
AC137615|AC137615.1 Oryza sativa (japonica cultivar-group) chromosome 5 clone OSJNBa0053F13, *** SEQUENCING IN PROGRESS ***, 3 ordered pieces.    38   0.13   5
AX926714|AX926714.1 Sequence 4 from Patent WO03084989.             32   0.19   12
AY168955|AY168955.1 Mycoplasma fermentans strain PG18 ORF228 gene, complete cds; insertion sequence ISMf1, complete sequence; ORF700 gene, complete cds; ORF251 pseudogene and integrative conjugal element ICEF-IIA, partial sequence.    38   0.22   2
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    34   0.22   10
dna update 2004. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC123899_21(AC123899|pid:none) Medicago truncatula clone mth2-33...    99   5e-19
AC009273_19(AC009273|pid:none) Sequence of BAC T1N6 from Arabido...    98   8e-19
AK317367_1(AK317367|pid:none) Arabidopsis thaliana AT1G01780 mRN...    98   8e-19
AF116851_1(AF116851|pid:none) Nicotiana tabacum LIM domain prote...    96   2e-18
BC121434_1(BC121434|pid:none) Xenopus tropicalis epithelial prot...    96   3e-18
AK031698_1(AK031698|pid:none) Mus musculus 13 days embryo male t...    96   4e-18
AK049350_1(AK049350|pid:none) Mus musculus ES cells cDNA, RIKEN ...    96   4e-18
BT061506_1(BT061506|pid:none) Zea mays full-length cDNA clone ZM...    96   4e-18
AF307845_1(AF307845|pid:none) Mus musculus epithelial protein lo...    96   4e-18
BC031490_1(BC031490|pid:none) Mus musculus LIM domain and actin ...    96   4e-18
protein update 2009. 5.28
PSORT 

 psg: 0.75  gvh: 0.31  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SFL216 is nuc
5' end seq. ID SFL216F
5' end seq. 
>SFL216F.Seq
ATTTTCATTTTTTTTATCGCCTCACTTTTTATTTTTGTATTATATTCTCTCTTTATTTCT
TTTCTATATATAGATAAATTTTTTTTAGAAAAACTAAAAAAAAAACCTCAAACAATTAAT
ACAAAATGGTAAACATTGGTGGAACTCAAGAAAAATGTACTGCATGTGCTAAAACTGTAT
ACTTAACAGAAAAGATTGTAGTTGAAGATAAAGAAGATAAGAAAACTTTCCACAAATTAT
GTTTAAAATGTACTCACTGTAAGCTTACATTAAGTCTTGGTAACTATGCATCATTAAATG
GNGTATTCTATTGTAAACCACATTTCAAACAATTATTCGCCACCAAAGGTAACTACGATG
AAGGTTTCGGTAAATCAAAACATTCTGAAAAATGGACACCACAAGCCACCCCAACTGGTA
CTTCTTCATTCATTCCAGTTGAAGAATCAAAATCATCAGAAAAGAAAGAAACACCAACAA
CAATTTCATCAAAATTCTCAGGATCAACTGAAAAATGTAATCTCTGTAATAAGACAGTAT
ATTTAACAGAAAAGATTGTTGTTGAAGATAAAGAAGATAAGAAAGTTTTACATAAACAAT
GTTTGAAATGTACACATTGGAGNGGTGGATTAAATCTTGGTACCTATGCATCAATGAAAA
GGNGTATTCTATTGGAAACCACATTTCAAACAATTATTCGCCCCCAANGGTACCTNTGAT
GNAAGGTTT----------
Length of 5' end seq. 729
3' end seq. ID SFL216Z
3' end seq. 
>SFL216Z.Seq
----------TGCCAAGAAATTCTCAACTGGTAGTTCAGAGAAATGTCACGATTGTCAAA
AATCAGTTTACCTCACAGAGAAAGTCGTACTTGAGGAATTGGAAAACAAGAGAATCTTCC
ACAAGGCTTGTTTGAAATGTAGTAAATGTTCAGTCATTCTCACCTTGGGTACTTTGGTTC
AATTGGATGGTGTTATCTATTGTAAACCACACTTTAAAGAACTCTATGCCACTCAAGGTA
ATTTAGATGGTGGTTTCGGTAAACCAAAACACTCTGAAAAATGGGAAAATAACCCATTCC
CATATTTAGAAAAGGATAGCAGTTATGTACCATCCGTTGAAAAGGTTGAATCTCAAATGA
ATAATGCTTCATCTGAATCTGATGTTGATAGATTTAAAAATATTAACAATGATGATTCTG
ATAAACCAAGAAGACAACAACAACCAGTTTCTCAACCAGAAGAAGAACAACAACAACAAG
AAGAAGAAAAAGTTGAAGAACCAATCTCTGAAGAAGAAGAAAAAGTTGAAGAAAAAGTTG
AAGAACCAGTCGCTGAACAAGAAGAAGAAAAGGTTGAGGAACCAGTTGTTGCTGAAGAAG
AAAAAGTTGAAGAACCAGTTACTGAAGAAGAAAAAGTTGAAGAACCAGTCGTTGCCTCTA
AGAACCAGTNAAGAAGAAACAAAAGCNAAGAAGAAAACT
Length of 3' end seq. 689
Connected seq. ID SFL216P
Connected seq. 
>SFL216P.Seq
ATTTTCATTTTTTTTATCGCCTCACTTTTTATTTTTGTATTATATTCTCTCTTTATTTCT
TTTCTATATATAGATAAATTTTTTTTAGAAAAACTAAAAAAAAAACCTCAAACAATTAAT
ACAAAATGGTAAACATTGGTGGAACTCAAGAAAAATGTACTGCATGTGCTAAAACTGTAT
ACTTAACAGAAAAGATTGTAGTTGAAGATAAAGAAGATAAGAAAACTTTCCACAAATTAT
GTTTAAAATGTACTCACTGTAAGCTTACATTAAGTCTTGGTAACTATGCATCATTAAATG
GNGTATTCTATTGTAAACCACATTTCAAACAATTATTCGCCACCAAAGGTAACTACGATG
AAGGTTTCGGTAAATCAAAACATTCTGAAAAATGGACACCACAAGCCACCCCAACTGGTA
CTTCTTCATTCATTCCAGTTGAAGAATCAAAATCATCAGAAAAGAAAGAAACACCAACAA
CAATTTCATCAAAATTCTCAGGATCAACTGAAAAATGTAATCTCTGTAATAAGACAGTAT
ATTTAACAGAAAAGATTGTTGTTGAAGATAAAGAAGATAAGAAAGTTTTACATAAACAAT
GTTTGAAATGTACACATTGGAGNGGTGGATTAAATCTTGGTACCTATGCATCAATGAAAA
GGNGTATTCTATTGGAAACCACATTTCAAACAATTATTCGCCCCCAANGGTACCTNTGAT
GNAAGGTTT----------TGCCAAGAAATTCTCAACTGGTAGTTCAGAGAAATGTCACG
ATTGTCAAAAATCAGTTTACCTCACAGAGAAAGTCGTACTTGAGGAATTGGAAAACAAGA
GAATCTTCCACAAGGCTTGTTTGAAATGTAGTAAATGTTCAGTCATTCTCACCTTGGGTA
CTTTGGTTCAATTGGATGGTGTTATCTATTGTAAACCACACTTTAAAGAACTCTATGCCA
CTCAAGGTAATTTAGATGGTGGTTTCGGTAAACCAAAACACTCTGAAAAATGGGAAAATA
ACCCATTCCCATATTTAGAAAAGGATAGCAGTTATGTACCATCCGTTGAAAAGGTTGAAT
CTCAAATGAATAATGCTTCATCTGAATCTGATGTTGATAGATTTAAAAATATTAACAATG
ATGATTCTGATAAACCAAGAAGACAACAACAACCAGTTTCTCAACCAGAAGAAGAACAAC
AACAACAAGAAGAAGAAAAAGTTGAAGAACCAATCTCTGAAGAAGAAGAAAAAGTTGAAG
AAAAAGTTGAAGAACCAGTCGCTGAACAAGAAGAAGAAAAGGTTGAGGAACCAGTTGTTG
CTGAAGAAGAAAAAGTTGAAGAACCAGTTACTGAAGAAGAAAAAGTTGAAGAACCAGTCG
TTGCCTCTAAGAACCAGTNAAGAAGAAACAAAAGCNAAGAAGAAAACT
Length of connected seq. 1418
Full length Seq ID -
Full length Seq. -
Length of full length seq. -