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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL209
Library SF
(Link to library)
Clone ID SFL209
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID SFL209P
(Link to Original site)
Representative DNA sequence 
>SFL209 (SFL209Q) /CSM/SF/SFL2-A/SFL209Q.Seq.d/
AAATTNATTTTTATTNTACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAAANCCCCTTTTTNGAAAAAAAAAAX
XXXXXXXXXATGTAAAAACNAAANTTCAAGATAANGAAGGTATTCCCCCNGATCAACAAC
GTNTCATTTTNCGTTGGTAANCAATTNAGAANATGTTNGNACTCTCTCTGATTACAATAT
CCAAAAAGAATCNAACTNTCCCACTTGGTCNTCAGATTAAGAGGGTGGTATGNAAATCTT
TGTCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGA
AAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAAT
TTTCCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGG
AATCCNCTCTNCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCC
TCACTGGTAAGACTATTACACTNGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAG
CCAAAATNCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTA
AACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCC
ATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAA
CCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAG
ACAAAGAAGGTATTCCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nxflxyky*ihilffyil*HFIKKKKKKKXKKXPFXKKK---

---VKTKXQDXEGIPPDQQRXIXRW*xixxmxxlslitiskknxtxplgxqikrvvxksl
skh*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fsagkqledgrtlsdyniqke
sxlhlvlrlrggmqifvktltgktitlevegsdnienvkakxqdkegippdqqrlifagk
qledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvktkiqd
kegip
Translated Amino Acid sequence (All Frames) 
Frame A:
kxifixqilnsyfiflyivtfhkkkkkkkx*kxpfxekk---

---m*kxxfkixkvfpxinnvsfxvgxqxrxcxxsl*lqypkrixlshlvxrlrgwyxnl
cqninw*dhhs*s*glr*y*kc*nkdsrqrryptrptkinfplvnn*kmvvlslittfkr
nplxi*ssd*evvckflskpslvrllhxklkvlitlrmlkpkxktkkvfhqinnvsfspv
nnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkfk
tkkvf

Frame B:
nxflxyky*ihilffyil*HFIKKKKKKKXKKXPFXKKK---

---cknxxsr*xryspxsttxhfxlvxnxexvxtlsdyniqkesnxptwssd*eggmxif
vktltgktitlevegsdnienvktkiqdkegippdqqrlifrw*tirrwsysl*lqhskg
ixsxfspqikrwyanfcqnphw*dyytxs*rf**h*ec*sqnxrqrrystrsttshfrr*
tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*ecknknsr
qrrys

Frame C:
ixfyxtnikfifyffiycnis*kkkkkkxlkxplfxkkk---

---VKTKXQDXEGIPPDQQRXIXRW*xixxmxxlslitiskknxtxplgxqikrvvxksl
skh*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fsagkqledgrtlsdyniqke
sxlhlvlrlrggmqifvktltgktitlevegsdnienvkakxqdkegippdqqrlifagk
qledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvktkiqd
kegip
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL209 (SFL209Q) /CSM/SF/SFL2-A/SFL209Q.Seq.d/                       1330   0.0
SFB295 (SFB295Q) /CSM/SF/SFB2-D/SFB295Q.Seq.d/                       1227   0.0
CHB622 (CHB622Q) /CSM/CH/CHB6-A/CHB622Q.Seq.d/                       1225   0.0
AFO836 (AFO836Q) /CSM/AF/AFO8-B/AFO836Q.Seq.d/                       1219   0.0
CFB804 (CFB804Q) /CSM/CF/CFB8-A/CFB804Q.Seq.d/                       1217   0.0
CFG260 (CFG260Q) /CSM/CF/CFG2-C/CFG260Q.Seq.d/                       1213   0.0
CFF493 (CFF493Q) /CSM/CF/CFF4-D/CFF493Q.Seq.d/                       1213   0.0
SFK774 (SFK774Q) /CSM/SF/SFK7-D/SFK774Q.Seq.d/                       1209   0.0
CFJ521 (CFJ521Q) /CSM/CF/CFJ5-A/CFJ521Q.Seq.d/                       1207   0.0
CFA741 (CFA741Q) /CSM/CF/CFA7-B/CFA741Q.Seq.d/                       1207   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.   819   0.0    6
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.   811   0.0    6
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.   811   0.0    6
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   517   0.0    6
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   494   e-179  4
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   478   e-174  5
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   392   e-154  4
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   392   e-154  4
Z54197|Z54197.1 C.albicans UBI4 gene encoding polyubiquitin.      202   3e-84  6
CF181989|CF181989.1 ISO4G3F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.   161   3e-77  4
dna update 2004. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

B27806(B27806) ubiquitin (clone lambda229) - slime mold (Dictyos...   276   2e-92
A29584(A29584) ubiquitin precursor - pig (fragment)  &M18159_1(M...   265   3e-92
AF088848_1(AF088848|pid:none) Capsicum chinense polyubiquitin (U...   264   2e-91
AF528710_1(AF528710|pid:none) Arabidopsis thaliana ecotype Ita-0...   262   8e-90
I45964(I45964) polyubiquitin - bovine (fragment)  &M62428_1(M624...   265   6e-89
AL117514_1(AL117514|pid:none) Homo sapiens mRNA; cDNA DKFZp434K0...   265   6e-89
EU306853_1(EU306853|pid:none) Drosophila silvestris clone sv3E-T...   265   6e-89
AJ965268_1(AJ965268|pid:none) Eudiplodinium maggii partial mRNA ...   256   8e-89
AF285161_1(AF285161|pid:none) Mus musculus cell-line C3H/He poly...   285   2e-87
DQ216234_1(DQ216234|pid:none) Taeniopygia guttata clone 0058P001...   281   2e-87
protein update 2009. 5.28
PSORT 

 psg: 0.52  gvh: 0.11  alm: 0.81  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.79  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for SFL209 is nuc
5' end seq. ID SFL209F
5' end seq. 
>SFL209F.Seq
AAATTNATTTTTATTNTACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAAANCCCCTTTTTNGAAAAAAAAAA-
---------
Length of 5' end seq. 119
3' end seq. ID SFL209Z
3' end seq. 
>SFL209Z.Seq
----------ATGTAAAAACNAAANTTCAAGATAANGAAGGTATTCCCCCNGATCAACAA
CGTNTCATTTTNCGTTGGTAANCAATTNAGAANATGTTNGNACTCTCTCTGATTACAATA
TCCAAAAAGAATCNAACTNTCCCACTTGGTCNTCAGATTAAGAGGGTGGTATGNAAATCT
TTGTCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTG
AAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAA
TTTTCCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAG
GAATCCNCTCTNCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACC
CTCACTGGTAAGACTATTACACTNGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAA
GCCAAAATNCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGT
AAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTC
CATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAA
ACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAA
GACAAAGAAGGTATTCCA
Length of 3' end seq. 728
Connected seq. ID SFL209P
Connected seq. 
>SFL209P.Seq
AAATTNATTTTTATTNTACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAAANCCCCTTTTTNGAAAAAAAAAA-
---------ATGTAAAAACNAAANTTCAAGATAANGAAGGTATTCCCCCNGATCAACAAC
GTNTCATTTTNCGTTGGTAANCAATTNAGAANATGTTNGNACTCTCTCTGATTACAATAT
CCAAAAAGAATCNAACTNTCCCACTTGGTCNTCAGATTAAGAGGGTGGTATGNAAATCTT
TGTCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGA
AAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAAT
TTTCCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGG
AATCCNCTCTNCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCC
TCACTGGTAAGACTATTACACTNGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAG
CCAAAATNCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTA
AACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCC
ATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAA
CCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAG
ACAAAGAAGGTATTCCA
Length of connected seq. 847
Full length Seq ID -
Full length Seq. -
Length of full length seq. -