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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL195
Library SF
(Link to library)
Clone ID SFL195
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16317-1
Original site URL
Representative seq. ID SFL195P
(Link to Original site)
Representative DNA sequence 
>SFL195 (SFL195Q) /CSM/SF/SFL1-D/SFL195Q.Seq.d/
GTATACGGCCATACTAGGTTGGAAACACATCATCCCGTTCGATCTGATAAGTAAATCGAC
CTCAGGCCTTCCAAGTACTCTGGTTGGAGACAACAGGGGAACATAGGGTGCTGTATACTA
TTTTTTTAAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGTA
TTCATATTAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAAT
TTACAACCAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATT
TAGTGAACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATTAT
CAGACTATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCAC
CACAACAAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACAAC
GTCAACCACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAACA
ATCCTGAAGXXXXXXXXXXGNGAAATTTCAAGAATTATTGATANTAGATTTCATGGTATT
GNTAAAGGTGTGGGTACATCAAAAATTATAGGTAGAGTTCATAGTACAGACTTAAAACTT
GGCAACTCTTTATTCTCTGNATCATTATCTATCCTTCAAAATCAAGATGTTGACTTCATC
CTTGGTTTGGATATGTTAAAGCGTCNTCAAGTCATTTTGGATTTAAATAGAGGTGTATTA
CAAATTGCAAATGAAAAGATTGAATTCCTTCATGAAAAAGATTTAAAGGAGATTTTAAAT
AAAGAACAAAATGATTTAGAAGAAGATACTAAAAAAGCAACTGCTAATAGTCTTTCACCT
TCAACAACTTCAACCACTAACACTACCACTACCACTACTTCACCCCAAACTCCAACTAAT
GCCACTTCTCCTGTAAATAATAATAATAATAATAATAACAACAACAACAACACTAGACCT
CCAATTAATTCACCACCAGTTGCAAAGACACCGCAACCAACACCACAACCAACTACAAAT
CAATATGCTTCTGAATCCAATATCCAAACCCTTATTGGACTTGGTTGTTCAAGAGATAAG
GCAATTAGACTTTNACTCAAGCTAAAGGCAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
irpy*vgntssrsi**vnrpqafqvlwlettgehrvlytiflntlthk*ylsiyphtqsi
hingrnnnin*k*kfhsskfttrsns*rskt*sri*NHNLVNNQVLTLDGKVLDNEKKLS
DYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQPQQQRQPQRDPLNSPQDILDHFTNN
PE---

---EISRIIDXRFHGIXKGVGTSKIIGRVHSTDLKLGNSLFSXSLSILQNQDVDFILGLD
MLKRXQVILDLNRGVLQIANEKIEFLHEKDLKEILNKEQNDLEEDTKKATANSLSPSTTS
TTNTTTTTTSPQTPTNATSPVNNNNNNNNNNNNTRPPINSPPVAKTPQPTPQPTTNQYAS
ESNIQTLIGLGCSRDKAIRLXLKLKA
Translated Amino Acid sequence (All Frames) 
Frame A:
vyghtrlethhpvrsdk*idlrpskysgwrqqgnigccilff*th*hinniylsihtpkv
filmaeitisienenfiqvnlqpdqtvedlkrrvefetti**tikf*hlmeky*imkrny
qtiqlkvvisy**qkmf*ehhnkdhnnpnnhnnhnnhnnnvnhkeilsihhkif*ttlqt
ilk---

---xkfqellixdfmvlxkvwvhqkl*vefivqt*nlatlyslxhylsfkikmltsslvw
ic*svxksfwi*ievyyklqmkrlnsfmkki*rrf*iknkmi*kkilkkqllivfhlqql
qpltlplpllhpklqlmplll*iiiiiiitttttldlqlihhqlqrhrnqhhnqlqinml
lnpiskplldlvvqeirqldfxss*rq

Frame B:
ytailgwkhiipfdliskstsglpstlvgdnrgt*gavyyffkhtnt*iifiylsthpky
sy*wqk*qyqlkmkisfk*iynqikqlki*nve*nlkpqfseqssfnt*wksir**keii
rlfn*rw*flidnkkcfkstttkitttpttttttttttttsttkrssqfttryfrplykq
s*---

---xnfkny*x*iswyx*rcgyiknyr*ss*yrlktwqlfilxiiiypsksrc*lhpwfg
yvkassshfgfk*rcitnck*kd*ips*krfkgdfk*rtk*frrry*ksnc**sftfnnf
nh*hyhyhyftpnsn*chfsck*******qqqqh*tsn*fttsckdtatntttnyksicf
*iqypnpywtwlfkr*gn*txtqakg

Frame C:
irpy*vgntssrsi**vnrpqafqvlwlettgehrvlytiflntlthk*ylsiyphtqsi
hingrnnnin*k*kfhsskfttrsns*rskt*sri*NHNLVNNQVLTLDGKVLDNEKKLS
DYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQPQQQRQPQRDPLNSPQDILDHFTNN
PE---

---EISRIIDXRFHGIXKGVGTSKIIGRVHSTDLKLGNSLFSXSLSILQNQDVDFILGLD
MLKRXQVILDLNRGVLQIANEKIEFLHEKDLKEILNKEQNDLEEDTKKATANSLSPSTTS
TTNTTTTTTSPQTPTNATSPVNNNNNNNNNNNNTRPPINSPPVAKTPQPTPQPTTNQYAS
ESNIQTLIGLGCSRDKAIRLXLKLKA
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL195 (SFL195Q) /CSM/SF/SFL1-D/SFL195Q.Seq.d/                        866   0.0
VFE196 (VFE196Q) /CSM/VF/VFE1-D/VFE196Q.Seq.d/                        785   0.0
SFL211 (SFL211Q) /CSM/SF/SFL2-A/SFL211Q.Seq.d/                        785   0.0
CHD380 (CHD380Q) /CSM/CH/CHD3-D/CHD380Q.Seq.d/                        785   0.0
CHA270 (CHA270Q) /CSM/CH/CHA2-C/CHA270Q.Seq.d/                        785   0.0
CHA120 (CHA120Q) /CSM/CH/CHA1-A/CHA120Q.Seq.d/                        785   0.0
CFI315 (CFI315Q) /CSM/CF/CFI3-A/CFI315Q.Seq.d/                        785   0.0
AFJ439 (AFJ439Q) /CSM/AF/AFJ4-B/AFJ439Q.Seq.d/                        785   0.0
AFJ488 (AFJ488Q) /CSM/AF/AFJ4-D/AFJ488Q.Seq.d/                        773   0.0
SFJ734 (SFJ734Q) /CSM/SF/SFJ7-B/SFJ734Q.Seq.d/                        771   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

V00191|V00191.1 5S ribosomal RNA of slime mold (D. discoideum).   236   6e-58  1
M25202|M25202.1 D.discoideum 5S rRNA.                             236   6e-58  1
AY171066|AY171066.1 Dictyostelium discoideum extrachromosomal palindromic ribosomal RNA gene sequence, central segment.   236   6e-58  1
X67782|X67782.1 D.discoideum 5S ribosomal RNA gene.               236   6e-58  1
AY293825|AY293825.1 Dictyostelium discoideum transposon cluster; tRNA-Leu gene, 5S ribosomal RNA pseudogene, and tRNA-Ser gene, complete sequence.   192   8e-45  1
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    54   1e-11  11
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    44   2e-07  16
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    40   5e-05  8
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    38   2e-04  12
U30821|U30821.1 Cyanophora paradoxa cyanelle, complete genome.     30   2e-04  13
dna update 2004. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JB0) RecName: Full=Protein DDI1 homolog;                          253   7e-66
AM910995_387(AM910995|pid:none) Plasmodium knowlesi strain H chr...    92   3e-17
(Q4WGS4) RecName: Full=DNA damage-inducible protein 1;                 91   6e-17
(A1DCU5) RecName: Full=DNA damage-inducible protein 1;                 91   6e-17
(Q7S906) RecName: Full=DNA damage-inducible protein 1;                 91   1e-16
(Q6CFI3) RecName: Full=DNA damage-inducible protein 1;  &CR38212...    90   1e-16
AP007151_401(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...    90   2e-16
(Q2USD7) RecName: Full=DNA damage-inducible protein 1;                 90   2e-16
(A1CDT9) RecName: Full=DNA damage-inducible protein 1;                 90   2e-16
(Q10255) RecName: Full=Uncharacterized protein C56F8.07;               87   8e-16
protein update 2009. 5.28
PSORT 

 psg: 0.74  gvh: 0.41  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for SFL195 is nuc
5' end seq. ID SFL195F
5' end seq. 
>SFL195F.Seq
GTATACGGCCATACTAGGTTGGAAACACATCATCCCGTTCGATCTGATAAGTAAATCGAC
CTCAGGCCTTCCAAGTACTCTGGTTGGAGACAACAGGGGAACATAGGGTGCTGTATACTA
TTTTTTTAAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGTA
TTCATATTAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAAT
TTACAACCAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATT
TAGTGAACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATTAT
CAGACTATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCAC
CACAACAAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACAAC
GTCAACCACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAACA
ATCCTGAAG----------
Length of 5' end seq. 549
3' end seq. ID SFL195Z
3' end seq. 
>SFL195Z.Seq
----------GNGAAATTTCAAGAATTATTGATANTAGATTTCATGGTATTGNTAAAGGT
GTGGGTACATCAAAAATTATAGGTAGAGTTCATAGTACAGACTTAAAACTTGGCAACTCT
TTATTCTCTGNATCATTATCTATCCTTCAAAATCAAGATGTTGACTTCATCCTTGGTTTG
GATATGTTAAAGCGTCNTCAAGTCATTTTGGATTTAAATAGAGGTGTATTACAAATTGCA
AATGAAAAGATTGAATTCCTTCATGAAAAAGATTTAAAGGAGATTTTAAATAAAGAACAA
AATGATTTAGAAGAAGATACTAAAAAAGCAACTGCTAATAGTCTTTCACCTTCAACAACT
TCAACCACTAACACTACCACTACCACTACTTCACCCCAAACTCCAACTAATGCCACTTCT
CCTGTAAATAATAATAATAATAATAATAACAACAACAACAACACTAGACCTCCAATTAAT
TCACCACCAGTTGCAAAGACACCGCAACCAACACCACAACCAACTACAAATCAATATGCT
TCTGAATCCAATATCCAAACCCTTATTGGACTTGGTTGTTCAAGAGATAAGGCAATTAGA
CTTTNACTCAAGCTAAAGGCAA
Length of 3' end seq. 612
Connected seq. ID SFL195P
Connected seq. 
>SFL195P.Seq
GTATACGGCCATACTAGGTTGGAAACACATCATCCCGTTCGATCTGATAAGTAAATCGAC
CTCAGGCCTTCCAAGTACTCTGGTTGGAGACAACAGGGGAACATAGGGTGCTGTATACTA
TTTTTTTAAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGTA
TTCATATTAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAAT
TTACAACCAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATT
TAGTGAACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATTAT
CAGACTATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCAC
CACAACAAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACAAC
GTCAACCACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAACA
ATCCTGAAG----------GNGAAATTTCAAGAATTATTGATANTAGATTTCATGGTATT
GNTAAAGGTGTGGGTACATCAAAAATTATAGGTAGAGTTCATAGTACAGACTTAAAACTT
GGCAACTCTTTATTCTCTGNATCATTATCTATCCTTCAAAATCAAGATGTTGACTTCATC
CTTGGTTTGGATATGTTAAAGCGTCNTCAAGTCATTTTGGATTTAAATAGAGGTGTATTA
CAAATTGCAAATGAAAAGATTGAATTCCTTCATGAAAAAGATTTAAAGGAGATTTTAAAT
AAAGAACAAAATGATTTAGAAGAAGATACTAAAAAAGCAACTGCTAATAGTCTTTCACCT
TCAACAACTTCAACCACTAACACTACCACTACCACTACTTCACCCCAAACTCCAACTAAT
GCCACTTCTCCTGTAAATAATAATAATAATAATAATAACAACAACAACAACACTAGACCT
CCAATTAATTCACCACCAGTTGCAAAGACACCGCAACCAACACCACAACCAACTACAAAT
CAATATGCTTCTGAATCCAATATCCAAACCCTTATTGGACTTGGTTGTTCAAGAGATAAG
GCAATTAGACTTTNACTCAAGCTAAAGGCAA
Length of connected seq. 1161
Full length Seq ID -
Full length Seq. -
Length of full length seq. -