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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL182
Library SF
(Link to library)
Clone ID SFL182
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16294-1
Original site URL
Representative seq. ID SFL182P
(Link to Original site)
Representative DNA sequence 
>SFL182 (SFL182Q) /CSM/SF/SFL1-D/SFL182Q.Seq.d/
TATTTTCCATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAG
AATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAA
TTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAG
CAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAA
TTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTG
AAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATG
CATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTATGGTC
GTGGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTG
CAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGGAAGGTTCCGAAGAATC
TGGTTCAATCCATTTACCACAATATGTTGXXXXXXXXXXTAGATCGTGCTGAAAATCAAT
TAACTGGTGAAGTCGATAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAA
TCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAA
ATACCACTCCAGTCACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAA
CTATTTGCATCACCGGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGA
GAACTCACACCACCGTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATG
CATCAACTGTATTAAAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCT
TCAAAATGGATAAATGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAA
AAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAG
GTGGTTCCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTA
CTGGTTTATTAGGTCCAGGTTCAAATGCTCAGGTCCAAATGAATTCTTAGATATTCCATT
TGCCAAGAAATTATTAAGTTGTGTATTATTTATTAGAGCTCATTCCAAGTTNATTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
yfplikln*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmviqkk
qfny*rngs*hkvskvfhvklkelkd*hqlfsw*SKQPKLKKLKLYFYMVTWINNHHSLM
HGMKDYIHTKQSSRITNSMVVGGADDGYSTFASISAIKALQEQSIPHDRYVIIIGRFRRI
WFNPFTTIC---

---DRAENQLTGEVDKRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKDLTQ
LMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEIFET
NPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMKMLH
DRFNCQFIVTGLLGPGSNAQVQMNS*ifhlprny*vvyyllelipsxl
Translated Amino Acid sequence (All Frames) 
Frame A:
yfplikln*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmviqkk
qfny*rngs*hkvskvfhvklkelkd*hqlfsw*SKQPKLKKLKLYFYMVTWINNHHSLM
HGMKDYIHTKQSSRITNSMVVGGADDGYSTFASISAIKALQEQSIPHDRYVIIIGRFRRI
WFNPFTTIC---

---*ivlkin*lvksikdytlkfhniv*iksqnvlkf*akpsmmnshgtkiplqslki*p
n**stklgiqlfaspvlkvfhtlqmlvms*eltpplklvfvyhhhskqlmhqly*kkfsk
lihhmvlklpskwinvlqvgihhkldqn*kkh*eklqifslakttytwvkvvpshs*kcy
mtvsivnlsllvy*vqvqmlrsk*ilrysicqeiiklciiy*ssfqvx*

Frame B:
ifh**n*ik*nviky*ks*ri***smg*kyftntfkiy*nsksitii*crmgnqwlyrrs
nsiiremdlstryqryfm*n*kn*rintnyfhgsrsnqn*ks*nctfiwshg*tttth*c
mg*ritsiqsshqe*qtlwswvvqmmviqhllqslqskhcknnqfhmivm*lslegsees
gsihlpqyv---

---rsc*ksinw*sr*kitr*nsttsfrsnrkmc*nfrrnhl**ilmvqkyhssh*rfnp
iddqqnlvsnylhhrc*rysthfkcw*chenshhr*s*csfttiiqss*cincikrnfrn
*stiwc*sylqng*mcfrlgfttn*trirksirrsfksflwqrprihg*rwfhpihenvt
*pfqlsiyrywfirsrfkcsgpnefldipfakkllscvlfirahskxi

Frame C:
fsidkiklnkmslniekvkefsnkawdenilptlskyieipnqsplydaewatngyteea
iqllekwilaqgikgisceikriegltpiifmvveatktekvktvllyghmdkqppltda
wdeglhpykaviknnklygrgwcr*wlfnicfnlcnqsiartinst*slcnyhwkvpknl
vqsiyhnml---

---DRAENQLTGEVDKRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKDLTQ
LMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEIFET
NPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMKMLH
DRFNCQFIVTGLLGPGSNAQVQMNS*ifhlprny*vvyyllelipsxl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL182 (SFL182Q) /CSM/SF/SFL1-D/SFL182Q.Seq.d/                       2300   0.0
CFG835 (CFG835Q) /CSM/CF/CFG8-B/CFG835Q.Seq.d/                       1316   0.0
AFB648 (AFB648Q) /CSM/AF/AFB6-B/AFB648Q.Seq.d/                       1316   0.0
AFJ819 (AFJ819Q) /CSM/AF/AFJ8-A/AFJ819Q.Seq.d/                       1302   0.0
AFD881 (AFD881Q) /CSM/AF/AFD8-D/AFD881Q.Seq.d/                       1296   0.0
SFG389 (SFG389Q) /CSM/SF/SFG3-D/SFG389Q.Seq.d/                       1292   0.0
AFB802 (AFB802Q) /CSM/AF/AFB8-A/AFB802Q.Seq.d/                       1291   0.0
VFI168 (VFI168Q) /CSM/VF/VFI1-C/VFI168Q.Seq.d/                       1285   0.0
SFI811 (SFI811Q) /CSM/SF/SFI8-A/SFI811Q.Seq.d/                       1285   0.0
CFJ404 (CFJ404Q) /CSM/CF/CFJ4-A/CFJ404Q.Seq.d/                       1285   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    40   0.011  9
AL354810|AL354810.8 Human DNA sequence from clone RP11-527N12 on chromosome 13.    38   0.069  10
AX489336|AX489336.1 Sequence 6636 from Patent WO02053728.          36   0.27   3
CK266014|CK266014.1 EST712092 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAC219 3' end, mRNA sequence.    48   0.31   1
CK261663|CK261663.1 EST707741 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABB34 3' end, mRNA sequence.    48   0.31   1
BI433279|BI433279.1 EST536040 P. infestans-challenged potato leaf, compatible reaction Solanum tuberosum cDNA clone PPCBC62 5' sequence, mRNA sequence.    48   0.31   1
BQ046631|BQ046631.1 EST595749 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI15C18 5' end, mRNA sequence.    48   0.31   1
BI435717|BI435717.1 EST538478 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCI86 5' sequence, mRNA sequence.    48   0.31   1
BI435853|BI435853.1 EST538614 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCL22 5' sequence, mRNA sequence.    48   0.31   1
BG591390|BG591390.1 EST499232 P. infestans-challenged leaf Solanum tuberosum cDNA clone BPLI8D17 5' sequence, mRNA sequence.    48   0.31   1
dna update 2004. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM743169_1072(AM743169|pid:none) Stenotrophomonas maltophilia K2...   181   6e-47
AM746676_157(AM746676|pid:none) Sorangium cellulosum 'So ce 56' ...   184   8e-47
CP000113_526(CP000113|pid:none) Myxococcus xanthus DK 1622, comp...   180   1e-46
CP001111_978(CP001111|pid:none) Stenotrophomonas maltophilia R55...   179   2e-46
AM039952_3097(AM039952|pid:none) Xanthomonas campestris pv. vesi...   181   2e-46
AE008923_2905(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   179   5e-46
DQ384330_1(DQ384330|pid:none) Hartmannella vermiformis succinyl-...   153   7e-43
CP000267_395(CP000267|pid:none) Rhodoferax ferrireducens T118, c...   164   7e-42
AE016828_97(AE016828|pid:none) Coxiella burnetii RSA 493, comple...   163   7e-42
CP001020_1727(CP001020|pid:none) Coxiella burnetii CbuK_Q154, co...   163   7e-42
protein update 2009. 5.28
PSORT 

 psg: 0.90  gvh: 0.29  alm: 0.48  top: 0.47  tms: 0.00  mit: 0.29  mip: 0.08
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for SFL182 is cyt
5' end seq. ID SFL182F
5' end seq. 
>SFL182F.Seq
TATTTTCCATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAG
AATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAA
TTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAG
CAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAA
TTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTG
AAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATG
CATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTATGGTC
GTGGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTG
CAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGGAAGGTTCCGAAGAATC
TGGTTCAATCCATTTACCACAATATGTTG----------
Length of 5' end seq. 569
3' end seq. ID SFL182Z
3' end seq. 
>SFL182Z.Seq
----------TAGATCGTGCTGAAAATCAATTAACTGGTGAAGTCGATAAAAGATTACAC
GTTGAAATTCCACAACATCGTTTAGATCAAATCGCAAAATGTGCTGAAATTTTAGGCGAA
ACCATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAAAGATTTAACC
CAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGCTGAAGGTATT
CCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAAAGTTAGTGTT
CGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGAAATTTTCGAA
ACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGCTTCAGGTTGG
GATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTTCAAATCTTTTC
TTTGGCAAAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCATTCATGAAAATGTTA
CATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCT
CAGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTATTAT
TTATTAGAGCTCATTCCAAGTTNATTAA
Length of 3' end seq. 678
Connected seq. ID SFL182P
Connected seq. 
>SFL182P.Seq
TATTTTCCATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAG
AATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAA
TTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAG
CAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAA
TTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTG
AAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATG
CATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTATGGTC
GTGGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTG
CAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGGAAGGTTCCGAAGAATC
TGGTTCAATCCATTTACCACAATATGTTG----------TAGATCGTGCTGAAAATCAAT
TAACTGGTGAAGTCGATAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAA
TCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAA
ATACCACTCCAGTCACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAA
CTATTTGCATCACCGGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGA
GAACTCACACCACCGTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATG
CATCAACTGTATTAAAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCT
TCAAAATGGATAAATGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAA
AAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAG
GTGGTTCCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTA
CTGGTTTATTAGGTCCAGGTTCAAATGCTCAGGTCCAAATGAATTCTTAGATATTCCATT
TGCCAAGAAATTATTAAGTTGTGTATTATTTATTAGAGCTCATTCCAAGTTNATTAA
Length of connected seq. 1247
Full length Seq ID -
Full length Seq. -
Length of full length seq. -