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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI655
Library AF
(Link to library)
Clone ID AFI655
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10768-1
Original site URL
Representative seq. ID AFI655P
(Link to Original site)
Representative DNA sequence 
>AFI655 (AFI655Q) /CSM/AF/AFI6-C/AFI655Q.Seq.d/
ATTTATAATTTTATATATTTGAATACATAATTTATATTTATTATTTTATAAATATATATT
TTTAATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAGAA
GAAGAAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAGTT
ACAGTTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTATT
AATGCTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTTAT
GATCCAGGTTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTGAT
AAAGGAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACTTT
TTAGAGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATGGT
TGGAGTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGAAA
ACATTTAGATATGATGCTCATCCAATGGGTATGTTAATXXXXXXXXXXCGTGCATTGNAA
ATTTTTATTCATTCTTCATGCAGATCATGAATTAAATTGTTCAACCGCTGCAATGAGACA
TATTTCATCATCAGGTACCGATCCATACACAAGTGTTGCTGGTGCCGCTGGTGCATTATA
TGGTCCATCTCATGGTGGTGCCAATGAGGCCGTATTGGATATGTTAATTCATATCGGTTC
CAAAGAGAATATTCCACAATTCATTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGG
TTTCGGTCATAGAATCTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGC
TTATGAAGTTTTCGAAAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGA
GAAACAAGCCTTGGAAGATGAATACTTTGTAAGCAGAAAACTTTATCCAAATGTTGATTT
CTACTCTGGTCTCATCTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATT
CACTATTCCACGTGCCGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGA
AACTAAAATCTATCGTCCACGTCAAGTTTACAAAGGTGAATGGTTTAGAAACTATGTCCC
AATCGATGGTAGACCACCAGCTAAAGTTCGTTCTCAGGATAGTTATTCGTCTGCTACCAC
TAAAAGATATTCAAAAGTTACTTCTCATTAAAATTTTAAAATTTAAAATTTAAGTTTTCA
T
sequence update 2001. 6. 2
Translated Amino Acid sequence 
iynfiylnt*fifiil*IYIFNMAHIDRINILANHLSKEEEEENELLTSPVSADKKKETV
TVTDNRNGKSYDFKIKNDTINALNFKELQLAKGDGGLMIYDPGFQNTAVVTSYITYIDGD
KGILRYRGYPIEELAERSNFLEVAYLLINGNLPNKSQLDGWSNKIMTHTFLHENLVGLMK
TFRYDAHPMGML---

---VHXKFLFILHADHELNCSTAAMRHISSSGTDPYTSVAGAAGALYGPSHGGANEAVLD
MLIHIGSKENIPQFISDVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEVFESLGKEPLI
EVATELEKQALEDEYFVSRKLYPNVDFYSGLIYKAMGFPTDMFPVLFTIPRAVGWLAHWV
EHLEDPETKIYRPRQVYKGEWFRNYVPIDGRPPAKVRSQDSYSSATTKRYSKVTSH*nfk
i*nlsfh
Translated Amino Acid sequence (All Frames) 
Frame A:
iynfiylnt*fifiil*IYIFNMAHIDRINILANHLSKEEEEENELLTSPVSADKKKETV
TVTDNRNGKSYDFKIKNDTINALNFKELQLAKGDGGLMIYDPGFQNTAVVTSYITYIDGD
KGILRYRGYPIEELAERSNFLEVAYLLINGNLPNKSQLDGWSNKIMTHTFLHENLVGLMK
TFRYDAHPMGML---

---ralxifihsscrs*iklfnrcnetyfiiryrsihkccwcrwciiwsiswwcq*grig
yvnsyrfqreystihfrckeqkeeingfrs*nlqkl*sksqdhs*ssl*sfrkfr*rtid
*ssh*vgetslgr*ilckqktlskc*fllwshl*sngfpn*yvpsfihystcrwlvsslg
rtfrrsrn*nlsstsslqr*mv*klcpnrw*tts*ssfsg*lfvcyh*kifksyfslkf*
nlkfkfs

Frame B:
fiilyi*ihnlyllfykyifliwhilivliylpiifqrkkkkkmny*lhqfllikkkrql
qlqiiemvkamisklkmtllml*tlknyn*pkvmvv**fmiqvskiqql*hhtlhilmvi
kef*dteviqlkn*qkdqtf*rllif*smvtyqinhn*mvgvikl*lilffmkiwlvl*k
hldmmliqwvc*---

---VHXKFLFILHADHELNCSTAAMRHISSSGTDPYTSVAGAAGALYGPSHGGANEAVLD
MLIHIGSKENIPQFISDVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEVFESLGKEPLI
EVATELEKQALEDEYFVSRKLYPNVDFYSGLIYKAMGFPTDMFPVLFTIPRAVGWLAHWV
EHLEDPETKIYRPRQVYKGEWFRNYVPIDGRPPAKVRSQDSYSSATTKRYSKVTSH*nfk
i*nlsfh

Frame C:
l*fyifeyiiyiyyfiniyf*ygty*sy*ytcqssfkgrrrrk*iinftsfc**kkrdsy
syr**kw*kl*fqn*k*hy*cfkl*ritisqr*wwfndl*srfpkysscniihyiy*w**
rnfkiqrlsn*risrkiklfrgclsfnqw*ltk*itirwle**nydsyfss*kfgwsyen
i*i*cssngyvn---

---cixnfysffmqimn*ivqplq*difhhqvpihtqvllvplvhymvhlmvvpmrpywi
c*fisvpkrifhnsfqm*rakrrn*wvsviestktmiqeprsfve*lmkfskv*vknh*l
k*plswrnkpwkmntl*aenfiqmlistlvssikqwvsqlicsqfyslfhvplvg*lig*
ni*kiqklksivhvkftkvngletmsqsmvdhqlkfvlrivirllplkdiqklllikilk
fki*vf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI655 (AFI655Q) /CSM/AF/AFI6-C/AFI655Q.Seq.d/                       2315   0.0
AFM803 (AFM803Q) /CSM/AF/AFM8-A/AFM803Q.Seq.d/                       1417   0.0
SSF821 (SSF821Q) /CSM/SS/SSF8-A/SSF821Q.Seq.d/                       1386   0.0
SSK530 (SSK530Q) /CSM/SS/SSK5-B/SSK530Q.Seq.d/                       1366   0.0
AFJ471 (AFJ471Q) /CSM/AF/AFJ4-C/AFJ471Q.Seq.d/                       1336   0.0
AFN717 (AFN717Q) /CSM/AF/AFN7-A/AFN717Q.Seq.d/                       1328   0.0
AFK171 (AFK171Q) /CSM/AF/AFK1-C/AFK171Q.Seq.d/                       1312   0.0
AFJ723 (AFJ723Q) /CSM/AF/AFJ7-A/AFJ723Q.Seq.d/                       1308   0.0
AFJ440 (AFJ440Q) /CSM/AF/AFJ4-B/AFJ440Q.Seq.d/                       1289   0.0
AFC579 (AFC579Q) /CSM/AF/AFC5-D/AFC579Q.Seq.d/                       1255   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY124377|AY124377.1 Dictyostelium discoideum citrate synthase (cshA) mRNA, complete cds.  1384   0.0    2
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.    80   2e-69  13
AB109907|AB109907.1 Tetrahymena thermophila tgCS mRNA for putative citrate synthase, complete cds.   101   2e-40  7
AF310892|AF310892.1 Dictyostelium discoideum citrate synthase (gltA) gene, partial cds; RacF2 (racF2) gene, complete cds; and unknown gene.    76   4e-21  2
AL385088|AL385088.1 Medicago truncatula EST MtBC26C06F1 : T3 end of clone MtBC26C06 of  cDNA library MtBC from arbuscular mycorrhiza of cultivar Jemalong of Medicago truncatula (barrel medic).   103   4e-21  2
Z70012|Z70012.1 Bartonella sp. gltA gene (strain N40).             52   7e-12  3
CV049517|CV049517.1 EST 14834 Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bgh001o07q 5', mRNA sequence.    62   7e-10  2
CV052502|CV052502.1 EST 11950 Half-Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bge016g02q 5', mRNA sequence.    62   7e-10  2
BU044708|BU044708.1 PP_LEa0020E04f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0020E04f, mRNA sequence.    62   7e-10  2
BU040616|BU040616.1 PP_LEa0006L02f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0006L02f, mRNA sequence.    62   8e-10  2
dna update 2004. 9.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK120755_1(AK120755|pid:none) Oryza sativa Japonica Group cDNA c...   315   3e-84
AB109907_1(AB109907|pid:none) Tetrahymena thermophila tgCS mRNA ...   315   3e-84
(P49299) RecName: Full=Citrate synthase, glyoxysomal;          E...   305   2e-81
(Q9SJH7) RecName: Full=Citrate synthase 3, peroxisomal;         ...   302   1e-80
AB334779_1(AB334779|pid:none) Glycine max mRNA for peroxisomal c...   301   3e-80
CR954217_232(CR954217|pid:none) Ostreococcus tauri strain OTTH05...   300   1e-79
CP000096_332(CP000096|pid:none) Pelodictyon luteolum DSM 273, co...   297   6e-79
CP000607_394(CP000607|pid:none) Chlorobium phaeovibrioides DSM 2...   296   8e-79
CP001110_2246(CP001110|pid:none) Pelodictyon phaeoclathratiforme...   296   1e-78
AB167269_1(AB167269|pid:none) Chlorobium limicola cit gene for c...   296   1e-78
protein update 2009. 4.23
PSORT 

 psg: 0.76  gvh: 0.30  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for AFI655 is cyt
5' end seq. ID AFI655F
5' end seq. 
>AFI655F.Seq
ATTTATAATTTTATATATTTGAATACATAATTTATATTTATTATTTTATAAATATATATT
TTTAATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAGAA
GAAGAAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAGTT
ACAGTTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTATT
AATGCTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTTAT
GATCCAGGTTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTGAT
AAAGGAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACTTT
TTAGAGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATGGT
TGGAGTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGAAA
ACATTTAGATATGATGCTCATCCAATGGGTATGTTAAT----------
Length of 5' end seq. 578
3' end seq. ID AFI655Z
3' end seq. 
>AFI655Z.Seq
----------CGTGCATTGNAAATTTTTATTCATTCTTCATGCAGATCATGAATTAAATT
GTTCAACCGCTGCAATGAGACATATTTCATCATCAGGTACCGATCCATACACAAGTGTTG
CTGGTGCCGCTGGTGCATTATATGGTCCATCTCATGGTGGTGCCAATGAGGCCGTATTGG
ATATGTTAATTCATATCGGTTCCAAAGAGAATATTCCACAATTCATTTCAGATGTAAAGA
GCAAAAAGAAGAAATTAATGGGTTTCGGTCATAGAATCTACAAAAACTATGATCCAAGAG
CCAAGATCATTCGTAGAGTAGCTTATGAAGTTTTCGAAAGTTTAGGTAAAGAACCATTGA
TTGAAGTAGCCACTGAGTTGGAGAAACAAGCCTTGGAAGATGAATACTTTGTAAGCAGAA
AACTTTATCCAAATGTTGATTTCTACTCTGGTCTCATCTATAAAGCAATGGGTTTCCCAA
CTGATATGTTCCCAGTTTTATTCACTATTCCACGTGCCGTTGGTTGGTTAGCTCATTGGG
TAGAACATTTAGAAGATCCAGAAACTAAAATCTATCGTCCACGTCAAGTTTACAAAGGTG
AATGGTTTAGAAACTATGTCCCAATCGATGGTAGACCACCAGCTAAAGTTCGTTCTCAGG
ATAGTTATTCGTCTGCTACCACTAAAAGATATTCAAAAGTTACTTCTCATTAAAATTTTA
AAATTTAAAATTTAAGTTTTCAT
Length of 3' end seq. 733
Connected seq. ID AFI655P
Connected seq. 
>AFI655P.Seq
ATTTATAATTTTATATATTTGAATACATAATTTATATTTATTATTTTATAAATATATATT
TTTAATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAGAA
GAAGAAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAGTT
ACAGTTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTATT
AATGCTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTTAT
GATCCAGGTTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTGAT
AAAGGAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACTTT
TTAGAGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATGGT
TGGAGTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGAAA
ACATTTAGATATGATGCTCATCCAATGGGTATGTTAAT----------CGTGCATTGNAA
ATTTTTATTCATTCTTCATGCAGATCATGAATTAAATTGTTCAACCGCTGCAATGAGACA
TATTTCATCATCAGGTACCGATCCATACACAAGTGTTGCTGGTGCCGCTGGTGCATTATA
TGGTCCATCTCATGGTGGTGCCAATGAGGCCGTATTGGATATGTTAATTCATATCGGTTC
CAAAGAGAATATTCCACAATTCATTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGG
TTTCGGTCATAGAATCTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGC
TTATGAAGTTTTCGAAAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGA
GAAACAAGCCTTGGAAGATGAATACTTTGTAAGCAGAAAACTTTATCCAAATGTTGATTT
CTACTCTGGTCTCATCTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATT
CACTATTCCACGTGCCGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGA
AACTAAAATCTATCGTCCACGTCAAGTTTACAAAGGTGAATGGTTTAGAAACTATGTCCC
AATCGATGGTAGACCACCAGCTAAAGTTCGTTCTCAGGATAGTTATTCGTCTGCTACCAC
TAAAAGATATTCAAAAGTTACTTCTCATTAAAATTTTAAAATTTAAAATTTAAGTTTTCA
T
Length of connected seq. 1311
Full length Seq ID -
Full length Seq. -
Length of full length seq. -