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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFL144
Library SF
(Link to library)
Clone ID SFL144
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFL144P
(Link to Original site)
Representative DNA sequence 
>SFL144 (SFL144Q) /CSM/SF/SFL1-B/SFL144Q.Seq.d/
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTNAAAGTG
ATATGAAACATCGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTT
TAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACAT
TTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGTG
ATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAAT
TTAAACGTAAACATAAAAAAGATATTATGGGTAATTCAACGTGCTGXXXXXXXXXXTAGA
TGTTGCACCATTATCAATGGGTTTAGAAACTGCTGGTGGTGTTATGACAACTCTTATTCC
AAGAAATACAACAATTCCATGTAAGAAAACTCAAACCTTCTCTACATACTCTGATAATCA
ACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTT
ATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGA
AGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAAC
TGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAAT
TGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCG
TGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGA
TGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGA
AAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATNAAGATAA
GATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATNAGTAACTCTATCAAATAGATTTC
TTAAAAAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
VFDAKRLIGRKFSDKEVXSDMKHRPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVLL
KMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYGL
EKKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVDEF
KRKHKKDIMGNSTC---

---DVAPLSMGLETAGGVMTTLIPRNTTIPCKKTQTFSTYSDNQPGVLIQVYEGERAMTK
DNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLS
KEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIE
SETESVLKWLESNQTAEKDEYXDKMKALEAVVNPIXSNSIK*is*ki
Translated Amino Acid sequence (All Frames) 
Frame A:
qylmlnv*lvvnlvtkkxkvi*nighsklfqrmvishifklnskvklkfshqkkslqwys
*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*mfnvllmnqllllllmv
*krkvlvkrif*fsi*vvvhlmflslplkmvylklkpplvili*vvkiliivsfltllmn
lnvnikkilwviqra---

---*mlhhyqwv*kllvvl*qllfqeiqqfhvrklkpslhtliinqvy*fkfmkvnvl*p
kiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*myqlkinqlvtnkrlqlpmikvvf
qkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsllkiqlkmkklqpkfqiqinqll
nlklkvysng*nqikpqkrmnxkir*kp*kqlsiqsxvtlsnrflkk

Frame B:
si*c*tfnws*i**qrsxk*yetsaiqsyskgw**atyss*iqr*n*sfltrrnlfngtl
knernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkikcstyy**tncccyclwf
rkerfw*keyfnfrfrwwyi*cfspyh*rwci*s*shrw*ysfrw*rf**ssrfslc**i
*t*t*kryyg*fnvl---

---rcctiingfrncwwcydnsyskkynnsm*ensnllyil**strcinssl*r*tcydq
r**fig*i*tfwyststkrctts*syi*c*ckwylkcis*r*inw*qtkdynyq**rssf
krrn*kngc*creiqttr*ttkrsc*ike*igklcihc*kfn*r*kscsqnfrfr*iny*
i*n*kctqmvriksnrrkg*ixr*deslrsscqsnhx*lyqidflkn

Frame C:
VFDAKRLIGRKFSDKEVXSDMKHRPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVLL
KMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYGL
EKKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVDEF
KRKHKKDIMGNSTC---

---DVAPLSMGLETAGGVMTTLIPRNTTIPCKKTQTFSTYSDNQPGVLIQVYEGERAMTK
DNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLS
KEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIE
SETESVLKWLESNQTAEKDEYXDKMKALEAVVNPIXSNSIK*is*ki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL144 (SFL144Q) /CSM/SF/SFL1-B/SFL144Q.Seq.d/                       2440   0.0
SHE551 (SHE551Q) /CSM/SH/SHE5-C/SHE551Q.Seq.d/                       1273   0.0
SFG805 (SFG805Q) /CSM/SF/SFG8-A/SFG805Q.Seq.d/                       1273   0.0
CHQ192 (CHQ192Q) /CSM/CH/CHQ1-D/CHQ192Q.Seq.d/                       1267   0.0
SFB318 (SFB318Q) /CSM/SF/SFB3-A/SFB318Q.Seq.d/                       1265   0.0
AFE717 (AFE717Q) /CSM/AF/AFE7-A/AFE717Q.Seq.d/                       1265   0.0
SFC313 (SFC313Q) /CSM/SF/SFC3-A/SFC313Q.Seq.d/                       1257   0.0
CHL528 (CHL528Q) /CSM/CH/CHL5-B/CHL528Q.Seq.d/                       1227   0.0
VHB106 (VHB106Q) /CSM/VH/VHB1-A/VHB106Q.Seq.d/                       1223   0.0
VHP224 (VHP224Q) /CSM/VH/VHP2-A/VHP224Q.Seq.d/                       1217   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1269   0.0    4
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1261   0.0    4
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   285   0.0    8
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   285   e-179  12
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 133   e-133  12
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          133   e-133  12
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   133   e-133  12
AF278536|AF278536.1 Parastrongyloides trichosuri heat shock 70 protein gene, complete cds.   157   e-116  10
AJ310880|AJ310880.1 Cryptosporidium baileyi partial hsp70 gene for heat shock protein  70.   100   e-116  9
AF221539|AF221539.1 Cryptosporidium baileyi heat shock protein 70 (HSP70) gene, partial cds.   100   e-116  9
dna update 2004. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   384   e-105
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   384   e-105
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   347   7e-94
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   345   2e-93
AY582833_1(AY582833|pid:none) Chytriomyces confervae strain CBS ...   325   2e-87
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           323   8e-87
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   320   5e-86
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   320   5e-86
EU679414_1(EU679414|pid:none) Tetranychus cinnabarinus heat shoc...   318   2e-85
EU679412_1(EU679412|pid:none) Tetranychus cinnabarinus heat shoc...   317   7e-85
protein update 2009. 5.28
PSORT 

 psg: 0.56  gvh: 0.22  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.03
 nuc: 0.07  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for SFL144 is nuc
5' end seq. ID SFL144F
5' end seq. 
>SFL144F.Seq
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTNAAAGTG
ATATGAAACATCGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTT
TAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACAT
TTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGTG
ATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAAT
TTAAACGTAAACATAAAAAAGATATTATGGGTAATTCAACGTGCTG----------
Length of 5' end seq. 586
3' end seq. ID SFL144Z
3' end seq. 
>SFL144Z.Seq
----------TAGATGTTGCACCATTATCAATGGGTTTAGAAACTGCTGGTGGTGTTATG
ACAACTCTTATTCCAAGAAATACAACAATTCCATGTAAGAAAACTCAAACCTTCTCTACA
TACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACC
AAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGT
GTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCT
GAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTT
TCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAA
CAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAA
AATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATT
GAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGAT
GAATATNAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATNAGTAACTCT
ATCAAATAGATTTCTTAAAAAATA
Length of 3' end seq. 674
Connected seq. ID SFL144P
Connected seq. 
>SFL144P.Seq
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTNAAAGTG
ATATGAAACATCGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTT
TAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACAT
TTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGTG
ATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAAT
TTAAACGTAAACATAAAAAAGATATTATGGGTAATTCAACGTGCTG----------TAGA
TGTTGCACCATTATCAATGGGTTTAGAAACTGCTGGTGGTGTTATGACAACTCTTATTCC
AAGAAATACAACAATTCCATGTAAGAAAACTCAAACCTTCTCTACATACTCTGATAATCA
ACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTT
ATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGA
AGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAAC
TGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAAT
TGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCG
TGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGA
TGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGA
AAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATNAAGATAA
GATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATNAGTAACTCTATCAAATAGATTTC
TTAAAAAATA
Length of connected seq. 1260
Full length Seq ID -
Full length Seq. -
Length of full length seq. -