>SFL136 (SFL136Q) /CSM/SF/SFL1-B/SFL136Q.Seq.d/
XXXXXXXXXXATAGAAGCAAATAATGATTCATGTGTACATGGTATTTTAGTTTATTATCC
AGTATTTGGAGGTATGATGGATTCTTATTTACAAGATGTTGTTAGTCCAACTAAGGATAT
TGAAGGTTTATCAACCCAAAATCGTTTCAATCTCTATCACAACATTCGTTTCATGGATGG
CGAAACCGCTACCAAGAAATGTGTCATACCATGCACACCATTGGCAATGGTAAAGATAAT
TGATAATTTAGGAATCTACGATAAATCATTAGCAATGGGTGAGCATTTAAAAGGTAAGAC
TGTGACAATCGTCAATCGTAGTGAAATCGTGGGTCGTCCATTGGCTGCAATGTTGGCAAA
CGACGGTGCGATAGTGTATTCAATCGATATCAATGGCATCATCATATTTCAGTCTGGTAA
AAGACATGGTACTATTAAAATGTCTGAAACTAATGTTACACGTGAAGAAGCAATCTCAAA
ATCTGATATCCTAATATTGGGTGT

---EANNDSCVHGILVYYPVFGGMMDSYLQDVVSPTKDIEGLSTQNRFNLYHNIRFMDGE
TATKKCVIPCTPLAMVKIIDNLGIYDKSLAMGEHLKGKTVTIVNRSEIVGRPLAAMLAND
GAIVYSIDINGIIIFQSGKRHGTIKMSETNVTREEAISKSDILILG

Frame A:
---*kqimihvymvf*fiiqylev*wiliykmllvqlrilkvyqpkivsisittfvswma
kplprnvsyhahhwqw*r*lii*estinh*qwvsi*kvrl*qssivvkswvvhwlqcwqt
tvr*ciqsismassyfslvkdmvllkclklmlhvkkqsqnlis*ywv


Frame B:
---rsk**fmctwyfsllssiwrydgflftrcc*sn*gy*rfinpksfqslsqhsfhgwr
nryqemchtmhtigngkdn**frnlr*iisng*afkr*dcdnrqs**nrgssigcnvgkr
rcdsvfnryqwhhhisvw*ktwyy*nv*n*cyt*rsnlki*ypnigc


Frame C:
---EANNDSCVHGILVYYPVFGGMMDSYLQDVVSPTKDIEGLSTQNRFNLYHNIRFMDGE
TATKKCVIPCTPLAMVKIIDNLGIYDKSLAMGEHLKGKTVTIVNRSEIVGRPLAAMLAND
GAIVYSIDINGIIIFQSGKRHGTIKMSETNVTREEAISKSDILILG

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL136 (SFL136Q) /CSM/SF/SFL1-B/SFL136Q.Seq.d/                        979   0.0
VFA866 (VFA866Q) /CSM/VF/VFA8-C/VFA866Q.Seq.d/                        973   0.0
SSK575 (SSK575Q) /CSM/SS/SSK5-D/SSK575Q.Seq.d/                        898   0.0
SSJ381 (SSJ381Q) /CSM/SS/SSJ3-D/SSJ381Q.Seq.d/                        811   0.0
SSE878 (SSE878Q) /CSM/SS/SSE8-D/SSE878Q.Seq.d/                        722   0.0
VHP889 (VHP889Q) /CSM/VH/VHP8-D/VHP889Q.Seq.d/                         36   0.49
VHC848 (VHC848Q) /CSM/VH/VHC8-B/VHC848Q.Seq.d/                         36   0.49
VFK111 (VFK111Q) /CSM/VF/VFK1-A/VFK111Q.Seq.d/                         36   0.49
VSK491 (VSK491Q) /CSM/VS/VSK4-D/VSK491Q.Seq.d/                         34   1.9
VSK471 (VSK471Q) /CSM/VS/VSK4-C/VSK471Q.Seq.d/                         34   1.9

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.   979   0.0    1
AC009125|AC009125.5 Homo sapiens chromosome 16 clone RP11-492H8, WORKING DRAFT SEQUENCE, 60 unordered pieces.    38   0.46   3
AI096634|AI096634.1 qb57c10.x1 NCI_CGAP_Brn23 Homo sapiens cDNA clone IMAGE:1704210 3', mRNA sequence.    46   0.47   1
AW975114|AW975114.1 EST387220 MAGE resequences, MAGN Homo sapiens cDNA, mRNA sequence.    46   0.47   1
AI767001|AI767001.1 wi92c01.x1 NCI_CGAP_Kid12 Homo sapiens cDNA clone IMAGE:2400768 3', mRNA sequence.    46   0.47   1
AA658364|AA658364.1 nu22e04.s1 NCI_CGAP_Pr2 Homo sapiens cDNA clone IMAGE:1208766, mRNA sequence.    46   0.47   1
AL136366|AL136366.24 Human DNA sequence from clone RP11-413C16 on chromosome 9p22.3-23.    46   0.47   1
BD183913|BD183913.1 Novel genes associated with maintenance of smooth muscle cell differentiation.    44   1.9    1
AC098024|AC098024.7 Rattus norvegicus clone CH230-54C2, WORKING DRAFT SEQUENCE, 1 ordered piece.    34   2.8    4
BX120006|BX120006.10 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    42   5.1    3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86KU6) RecName: Full=Methylenetetrahydrofolate dehydrogenase [...   328   5e-89
FM992691_415(FM992691|pid:none) Candida dubliniensis CD36 chromo...   176   2e-43
AY973541_4(AY973541|pid:none) Hyaloperonospora parasitica isolat...   164   8e-40
CU633899_262(CU633899|pid:none) Podospora anserina genomic DNA c...   163   2e-39
BX842629_14(BX842629|pid:none) Neurospora crassa DNA linkage gro...   160   2e-38
AE017346_367(AE017346|pid:none) Cryptococcus neoformans var. neo...   158   6e-38
CR380953_86(CR380953|pid:none) Candida glabrata strain CBS138 ch...   157   1e-37
(Q02046) RecName: Full=Methylenetetrahydrofolate dehydrogenase [...   154   8e-37
CR382121_337(CR382121|pid:none) Kluyveromyces lactis strain NRRL...   152   5e-36
AE016817_614(AE016817|pid:none) Ashbya gossypii (= Eremothecium ...   150   2e-35

 psg: 0.75  gvh: 0.45  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.07  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for SFL136 is cyt

>SFL136Z.Seq
----------ATAGAAGCAAATAATGATTCATGTGTACATGGTATTTTAGTTTATTATCC
AGTATTTGGAGGTATGATGGATTCTTATTTACAAGATGTTGTTAGTCCAACTAAGGATAT
TGAAGGTTTATCAACCCAAAATCGTTTCAATCTCTATCACAACATTCGTTTCATGGATGG
CGAAACCGCTACCAAGAAATGTGTCATACCATGCACACCATTGGCAATGGTAAAGATAAT
TGATAATTTAGGAATCTACGATAAATCATTAGCAATGGGTGAGCATTTAAAAGGTAAGAC
TGTGACAATCGTCAATCGTAGTGAAATCGTGGGTCGTCCATTGGCTGCAATGTTGGCAAA
CGACGGTGCGATAGTGTATTCAATCGATATCAATGGCATCATCATATTTCAGTCTGGTAA
AAGACATGGTACTATTAAAATGTCTGAAACTAATGTTACACGTGAAGAAGCAATCTCAAA
ATCTGATATCCTAATATTGGGTGT