>SFK882 (SFK882Q) /CSM/SF/SFK8-D/SFK882Q.Seq.d/
GGCCTACTGGAAAATTCCATGTATATAAACAAATAAATAAAATCCAAGTTTGCAGAGATT
TATCTATTCATTTATTTGTTGTGCCATGTTGGAGTTCTCTTTTCAACAAAAGCGTTCATA
CCTTCTTTTTGGTCATGCTAAGTAAGTAAATTAAAAATTTAGTTAGAAATCATATTATAC
ATACATATACATATACATATATAAATAAATATTTAATTTTAAAAAATGGTAATAAAAATA
ATAGTAATAAAATAATGATACCTACAGTACCAAAAGTAGAATGGAACATTCTACGTTCGA
ATCTAATACCTTCTTGAAGAGTTAATTCATATGATGCATTTACTGCTTCTTTTGCCATTG
CAACTATTTATTAAAATTATTTTTAAAAATTAATTATAATTAGTTACTATTAATTATTAT
TATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAAGATTGAATA
AATAAAAATAAATAATTACCAGNTAATTGAGAATAAGAAGCGATTTTTTCANNCATTTTA
GNGGCXXXXXXXXXXGCTTCAGTTAAAAGTTCTTCAACTGGAACAACTTTACTAACTAAA
CCAGCTTTTTCAGCTTCAACTGCAGTTAAATTATTACCTGTTAAAACTAATTCCATAGCT
TTTGATTTACCAATTGCTCTAATTAAACGTTGTGTACCACCACAACCTGGAATAGTACCT
AATTTAATCTCTGGTTGACCAAATACTGCTTTTTCACCAGCGATAATAATATCGCACATC
ATTGCTAATTCACAACCACCACCTAATGCATAACCATTTACAGCTGCAATAATTGGTTTA
CGAATCTTGGTAAGGTCATGCCATTGTGCTAAAAGATCATTATTGTAAGCATCTGAAATG
AATTACTATAACATAAATTTGTTTCTTCTTTTTTTATTATTTATTTACTTACCAGCAAAT
GCTTTTTCACTACCAGTGATAATGATTGAACCAACATCTTTATCTTCTTGGAAGAGTTTA
ACAGCACTGTTAATTTCTGAAATTAAACCATCAGAAAGTGCATTTAATGATTTTGGTCTA
TTTAAAGTAACAAGACCAACACGACCTTTCTTTTCTGCTAAAATATATTGAAAACTTGAA
TCTATTCCATTATTGAACCAATTAAGAATTGATTTAATTGGTTAGTATTAGTANNTTCAG
AANTAAAAAA

ptgkfhvykqinkiqvcrdlsihlfvvpcwsslfnksvhtfflvmlsk*iknlvrnhiih
tytytyinkylilkngnknnsnkimiptvpkvewnilrsnlips*rvnsydaftasfaia
tiy*nyf*kliiisyy*lllllllllllllllllllrlnk*k*iitx*LRIRSDFFXHFX
G---

---ASVKSSSTGTTLLTKPAFSASTAVKLLPVKTNSIAFDLPIALIKRCVPPQPGIVPNL
ISG*pntafspaiiishiiansqpppna*pftaaiiglrilvrschcakrsll*asemny
yninlfllfllfiylpanafslpvimieptslsswksltalliseikpsesafndfglfk
vtrptrpffsakiy*klesipllnqlridlig*y*xxqx*k

Frame A:
gllensmyink*ikskfaeiylfiyllchvgvlfstkafipsfwsc*vsklki*leiily
ihihihi*ini*f*kmvikiivik**ylqyqk*ngtfyvri*ylleelihmmhlllllpl
qlfikiifkn*l*lvtinyyyyyyyyyyyyyyyyyy*d*inknk*lpxn*e*eaifsxil
x---

---ASVKSSSTGTTLLTKPAFSASTAVKLLPVKTNSIAFDLPIALIKRCVPPQPGIVPNL
ISG*pntafspaiiishiiansqpppna*pftaaiiglrilvrschcakrsll*asemny
yninlfllfllfiylpanafslpvimieptslsswksltalliseikpsesafndfglfk
vtrptrpffsakiy*klesipllnqlridlig*y*xxqx*k

Frame B:
aywkipci*tnk*npslqrfiysficcamlefsfqqkrsyllfghak*vn*kfs*ksyyt
yiyiyiyk*ifnfkkw**k****nndtystksrmehstfesntflks*fi*ciycffchc
nyllklflkinyn*llliiiiiiiiiiiiiiiiiiikie*ikinnyqxienkkrffxxf*
x---

---lqlkvlqleqly*lnqlfqlqlqlnyyllklip*lliyqll*lnvvyhhnle*yli*
slvdqillfhqr**yrtsllihnhhlmhnhlqlq*lvyesw*ghaivlkdhyckhlk*it
it*icffffyylftyqqmlfhyq***lnqhlyllgrv*qhc*flklnhqkvhlmilvylk
*qdqhdlsfllkyienlnlfhy*tn*eli*lvsisxfrxkk

Frame C:
ptgkfhvykqinkiqvcrdlsihlfvvpcwsslfnksvhtfflvmlsk*iknlvrnhiih
tytytyinkylilkngnknnsnkimiptvpkvewnilrsnlips*rvnsydaftasfaia
tiy*nyf*kliiisyy*lllllllllllllllllllrlnk*k*iitx*LRIRSDFFXHFX
G---

---fs*kffnwnnftn*tsffsfncs*iitc*n*fhsf*ftncsn*tlcttttwnst*fn
lwltkycfftsdnniahhc*ftttt*citiyscnnwftnlgkvmplc*kiiivsi*nell
*hkfvssffiiylltskcffttsdnd*tnififleefnstvnf*n*tirkci**fwsi*s
nktnttflfc*nilkt*iysiiepikn*fnwlvlvxsexk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK882 (SFK882Q) /CSM/SF/SFK8-D/SFK882Q.Seq.d/                       1324   0.0
VSH733 (VSH733Q) /CSM/VS/VSH7-B/VSH733Q.Seq.d/                        674   0.0
SLA886 (SLA886Q) /CSM/SL/SLA8-D/SLA886Q.Seq.d/                        674   0.0
VFJ252 (VFJ252Q) /CSM/VF/VFJ2-C/VFJ252Q.Seq.d/                        375   e-102
VSB719 (VSB719Q) /CSM/VS/VSB7-A/VSB719Q.Seq.d/                        313   3e-84
VSH396 (VSH396Q) /CSM/VS/VSH3-D/VSH396Q.Seq.d/                         46   0.001
VSF855 (VSF855Q) /CSM/VS/VSF8-C/VSF855Q.Seq.d/                         46   0.001
VFO382 (VFO382Q) /CSM/VF/VFO3-D/VFO382Q.Seq.d/                         46   0.001
VFK420 (VFK420Q) /CSM/VF/VFK4-A/VFK420Q.Seq.d/                         46   0.001
VFA881 (VFA881Q) /CSM/VF/VFA8-D/VFA881Q.Seq.d/                         46   0.001

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BI508464|BI508464.1 BB170027B10C05.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170027B10C05 5', mRNA sequence.    94   1e-17  2
BI503829|BI503829.1 BB170025B20F12 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170025B20F12 5', mRNA sequence.    94   1e-17  2
BP510432|BP510432.1 Hydra magnipapillata cDNA, clone:hmp_05393.    50   6e-14  4
CB409062|CB409062.1 IPG015A02_251026 JH III-treated male I. pini midguts Ips pini cDNA clone IPG015A02 5, mRNA sequence.    78   5e-13  2
AK114153|AK114153.1 Ciona intestinalis cDNA, clone:cicl041i19, full insert sequence.    62   2e-12  2
BW260399|BW260399.1 Ciona intestinalis cDNA, clone:cign024h12, 5' end, single read.    62   2e-12  2
AV889339|AV889339.1 Ciona intestinalis cDNA, clone:rcicl47h12, 3' end, single read.    62   2e-12  2
AV998975|AV998975.1 Ciona intestinalis cDNA, clone:cicl47h12, 5' end, single read.    62   2e-12  2
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    44   5e-10  13
CA755504| (NM_078623) enoyl Coenzyme A hydratase, short chain 1; Enoyl-CoA, mRNA sequence.    62   2e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXF1) RecName: Full=Probable enoyl-CoA hydratase, mitochondri...   327   5e-88
(P14604) RecName: Full=Enoyl-CoA hydratase, mitochondrial;      ...   235   2e-60
AY892211_1(AY892211|pid:none) Synthetic construct Homo sapiens c...   234   4e-60
AY889749_1(AY889749|pid:none) Synthetic construct Homo sapiens c...   234   4e-60
AB170787_1(AB170787|pid:none) Macaca fascicularis brain cDNA clo...   234   6e-60
(Q5R646) RecName: Full=Enoyl-CoA hydratase, mitochondrial;      ...   233   1e-59
(Q58DM8) RecName: Full=Enoyl-CoA hydratase, mitochondrial;      ...   229   2e-58
AY049961_1(AY049961|pid:none) Bos taurus enoyl-CoA hydratase pre...   229   2e-58
D13900_1(D13900|pid:none) Homo sapiens mRNA for mitochondrial sh...   228   4e-58
DQ213433_1(DQ213433|pid:none) Taeniopygia guttata clone 0058P005...   224   6e-57

 psg: 0.87  gvh: 0.50  alm: 0.42  top: 0.50  tms: 0.00  mit: 0.40  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for SFK882 is mit

>SFK882F.Seq
GGCCTACTGGAAAATTCCATGTATATAAACAAATAAATAAAATCCAAGTTTGCAGAGATT
TATCTATTCATTTATTTGTTGTGCCATGTTGGAGTTCTCTTTTCAACAAAAGCGTTCATA
CCTTCTTTTTGGTCATGCTAAGTAAGTAAATTAAAAATTTAGTTAGAAATCATATTATAC
ATACATATACATATACATATATAAATAAATATTTAATTTTAAAAAATGGTAATAAAAATA
ATAGTAATAAAATAATGATACCTACAGTACCAAAAGTAGAATGGAACATTCTACGTTCGA
ATCTAATACCTTCTTGAAGAGTTAATTCATATGATGCATTTACTGCTTCTTTTGCCATTG
CAACTATTTATTAAAATTATTTTTAAAAATTAATTATAATTAGTTACTATTAATTATTAT
TATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAAGATTGAATA
AATAAAAATAAATAATTACCAGNTAATTGAGAATAAGAAGCGATTTTTTCANNCATTTTA
GNGGC----------

>SFK882Z.Seq
----------GCTTCAGTTAAAAGTTCTTCAACTGGAACAACTTTACTAACTAAACCAGC
TTTTTCAGCTTCAACTGCAGTTAAATTATTACCTGTTAAAACTAATTCCATAGCTTTTGA
TTTACCAATTGCTCTAATTAAACGTTGTGTACCACCACAACCTGGAATAGTACCTAATTT
AATCTCTGGTTGACCAAATACTGCTTTTTCACCAGCGATAATAATATCGCACATCATTGC
TAATTCACAACCACCACCTAATGCATAACCATTTACAGCTGCAATAATTGGTTTACGAAT
CTTGGTAAGGTCATGCCATTGTGCTAAAAGATCATTATTGTAAGCATCTGAAATGAATTA
CTATAACATAAATTTGTTTCTTCTTTTTTTATTATTTATTTACTTACCAGCAAATGCTTT
TTCACTACCAGTGATAATGATTGAACCAACATCTTTATCTTCTTGGAAGAGTTTAACAGC
ACTGTTAATTTCTGAAATTAAACCATCAGAAAGTGCATTTAATGATTTTGGTCTATTTAA
AGTAACAAGACCAACACGACCTTTCTTTTCTGCTAAAATATATTGAAAACTTGAATCTAT
TCCATTATTGAACCAATTAAGAATTGATTTAATTGGTTAGTATTAGTANNTTCAGAANTA
AAAAA

>SFK882P.Seq
GGCCTACTGGAAAATTCCATGTATATAAACAAATAAATAAAATCCAAGTTTGCAGAGATT
TATCTATTCATTTATTTGTTGTGCCATGTTGGAGTTCTCTTTTCAACAAAAGCGTTCATA
CCTTCTTTTTGGTCATGCTAAGTAAGTAAATTAAAAATTTAGTTAGAAATCATATTATAC
ATACATATACATATACATATATAAATAAATATTTAATTTTAAAAAATGGTAATAAAAATA
ATAGTAATAAAATAATGATACCTACAGTACCAAAAGTAGAATGGAACATTCTACGTTCGA
ATCTAATACCTTCTTGAAGAGTTAATTCATATGATGCATTTACTGCTTCTTTTGCCATTG
CAACTATTTATTAAAATTATTTTTAAAAATTAATTATAATTAGTTACTATTAATTATTAT
TATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAAGATTGAATA
AATAAAAATAAATAATTACCAGNTAATTGAGAATAAGAAGCGATTTTTTCANNCATTTTA
GNGGC----------GCTTCAGTTAAAAGTTCTTCAACTGGAACAACTTTACTAACTAAA
CCAGCTTTTTCAGCTTCAACTGCAGTTAAATTATTACCTGTTAAAACTAATTCCATAGCT
TTTGATTTACCAATTGCTCTAATTAAACGTTGTGTACCACCACAACCTGGAATAGTACCT
AATTTAATCTCTGGTTGACCAAATACTGCTTTTTCACCAGCGATAATAATATCGCACATC
ATTGCTAATTCACAACCACCACCTAATGCATAACCATTTACAGCTGCAATAATTGGTTTA
CGAATCTTGGTAAGGTCATGCCATTGTGCTAAAAGATCATTATTGTAAGCATCTGAAATG
AATTACTATAACATAAATTTGTTTCTTCTTTTTTTATTATTTATTTACTTACCAGCAAAT
GCTTTTTCACTACCAGTGATAATGATTGAACCAACATCTTTATCTTCTTGGAAGAGTTTA
ACAGCACTGTTAATTTCTGAAATTAAACCATCAGAAAGTGCATTTAATGATTTTGGTCTA
TTTAAAGTAACAAGACCAACACGACCTTTCTTTTCTGCTAAAATATATTGAAAACTTGAA
TCTATTCCATTATTGAACCAATTAAGAATTGATTTAATTGGTTAGTATTAGTANNTTCAG
AANTAAAAAA