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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFK821
Library SF
(Link to library)
Clone ID SFK821
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U01009-1|Contig-U15657-1
Original site URL
Representative seq. ID SFK821P
(Link to Original site)
Representative DNA sequence 
>SFK821 (SFK821Q) /CSM/SF/SFK8-A/SFK821Q.Seq.d/
AATAATTATTCCTCATAATCCATGTATTTAAAAAAATAAAAAAATTATTCTTCATAATAC
ATGTATTTTAAAAAATAAAATATATATATATATAAATATATTTATTTATTCAAAATAAAA
TGAATAAAACTATCATACTTTTATTAATTTCAATTATTTTTGAAATAGTAATATCATCAT
CCGTTGATGGTAATTATCCAGCTGGAGGTTTATATTTTGGTGTATCATCAAATTTCTTGG
TTGGTGAATCTGAAAAGTTTACTCAATATGTTCAAGATGAATTATTAGCAATGGATTTGC
CAGATCAATCAGGTGATTCAGATGGAGTACAATATTCATTTACTCATTTTAAACTTGCAT
TAAATTTACAAGATTTCTTTTATGCACAATTAGGACCTGGTGTTTTTCAAATGGGATGGG
ATACTATTACATTTAATTTACAATGGGAATATGAAATTTGTGTTAAAAAAGTTGTAAAAC
TTTGTGAATCAGGCACAATTACAGTTTATACAGCAAGTGGTCAAAGTGTATCATTAGGAA
CTACATTAGATGTTTTATTTAATTCATCAAATGCAAAGATTGAGGCACCATCAACTGTTA
TGCCATTTGAGGCGGXXXXXXXXXXAAACACCATCCTATGTACCATACGATTCACAAATG
GAGTCTTTCACATCAGACTACTGTATCACTATCACTGGTTACTTTATTGAGACCCTGTTG
GATGCTGTATTGGTACCAGAGTTTCCAATGACAATTCAACCATCACAAATACCAGCTTCA
TCACCAGTTCAATTGAATACAAGTTCTGATTTCTTTAGTGGTATCGCTCCAAATTTAACC
TCTAAATATCCAAATGTTGGTATTCAAGTGAATTTAATGCCACCAACAATACCAACGGTT
ACGATCAATTCAAGTGCTATCATTTTAACAGATTTTGAAATTTCAACTTCATTTTTAGTG
TTAACTGATAATGATGATGCTATTCCTGTTTTCAATGTTTTGTTCAAATTTGATGCTGAA
ATCCAAACCACCCTTTATACTGATAGTGTCTCTGTTTTCTCTTTAAATTCAACATTACTA
TCAATAACACCAAATGTAACCATAACTGATTCAAACGTTGGTTCTGTCGATGCCACTGGT
TTTGTTCAACTAATTGAAATGGCACAATCTATAATTAAAATTCCATCAATAACTTATCCT
GTACCTTCAAAATATTCAATAACAAATGTAAATTCACAACTTGGTNATCAAATAATTCAA
CTTACTTTNATTTAATNAAAATTCAACNAATCAAAAATATCTTAAAAAATCTGTAAATTT
A
sequence update 2001. 6. 1
Translated Amino Acid sequence 
*lfliihvfkkikklffiihvf*kikyiyi*IYLFIQNKMNKTIILLLISIIFEIVISSS
VDGNYPAGGLYFGVSSNFLVGESEKFTQYVQDELLAMDLPDQSGDSDGVQYSFTHFKLAL
NLQDFFYAQLGPGVFQMGWDTITFNLQWEYEICVKKVVKLCESGTITVYTASGQSVSLGT
TLDVLFNSSNAKIEAPSTVMPFEA---

---TPSYVPYDSQMESFTSDYCITITGYFIETLLDAVLVPEFPMTIQPSQIPASSPVQLN
TSSDFFSGIAPNLTSKYPNVGIQVNLMPPTIPTVTINSSAIILTDFEISTSFLVLTDNDD
AIPVFNVLFKFDAEIQTTLYTDSVSVFSLNSTLLSITPNVTITDSNVGSVDATGFVQLIE
MAQSIIKIPSITYPVPSKYSITNVNSQLGXQIIQLTXI*xkfnxskis*kickf
Translated Amino Acid sequence (All Frames) 
Frame A:
nnyss*smylkk*knyss*ymyfkk*niyiykyiylfkik*iklsyfy*fqlflk**yhh
plmviiqlevyilvyhqiswlvnlksllnmfkmny*qwicqinqviqmeynihllilnlh
*iykisfmhn*dlvffkwdgillhliyngnmkfvlkkl*nfvnqaqlqfiqqvvkvyh*e
lh*mfylihqmqrlrhhqllchlrr---

---khhpmyhtihkwslshqttvslslvtllrpcwmlywyqsfq*qfnhhkyqlhhqfn*
iqvlislvvslqi*plniqmlvfk*i*chqqyqrlrsiqvlsf*qilkfqlhf*c*limm
mlflfsmfcsnlmlkskppfilivslfsl*iqhyyq*hqm*p*liqtlvlsmplvlfn*l
kwhnl*lkfhq*lilylqniq*qm*ihnlvik*fnllxfnxnstnqkylkksvnl

Frame B:
iiiphnpci*knkkiilhntcilknkiyiyinifiysk*ne*nyhtfinfnyf*nsniii
r*w*lsswrfifwciikflgw*i*kvysicsr*iisngfarsir*frwstifiysf*tci
kftrfllctirtwcfsngmgyyyi*ftmgi*nlc*kscktl*irhnyslyskwskciirn
yircfi*fikckd*gtincyai*g---

---ntilctirftngvfhirllyhyhwlly*dpvgccigtrvsndnstitntsfitssie
ykf*fl*wyrskfnl*iskcwyssefnatnntngydqfkcyhfnrf*nfnfifsvn****
cyscfqcfvqi*c*npnhply**clcflfkfnitinntkcnhn*fkrwfcrchwfcstn*
ngtiyn*nsinnlsctfkifnnkckfttwxsnnstyfxlxkiqxiknilknl*i

Frame C:
*lfliihvfkkikklffiihvf*kikyiyi*IYLFIQNKMNKTIILLLISIIFEIVISSS
VDGNYPAGGLYFGVSSNFLVGESEKFTQYVQDELLAMDLPDQSGDSDGVQYSFTHFKLAL
NLQDFFYAQLGPGVFQMGWDTITFNLQWEYEICVKKVVKLCESGTITVYTASGQSVSLGT
TLDVLFNSSNAKIEAPSTVMPFEA---

---TPSYVPYDSQMESFTSDYCITITGYFIETLLDAVLVPEFPMTIQPSQIPASSPVQLN
TSSDFFSGIAPNLTSKYPNVGIQVNLMPPTIPTVTINSSAIILTDFEISTSFLVLTDNDD
AIPVFNVLFKFDAEIQTTLYTDSVSVFSLNSTLLSITPNVTITDSNVGSVDATGFVQLIE
MAQSIIKIPSITYPVPSKYSITNVNSQLGXQIIQLTXI*xkfnxskis*kickf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK821 (SFK821Q) /CSM/SF/SFK8-A/SFK821Q.Seq.d/                       2282   0.0
SFJ478 (SFJ478Q) /CSM/SF/SFJ4-D/SFJ478Q.Seq.d/                       1227   0.0
SFJ328 (SFJ328Q) /CSM/SF/SFJ3-B/SFJ328Q.Seq.d/                       1116   0.0
SFK638 (SFK638Q) /CSM/SF/SFK6-B/SFK638Q.Seq.d/                         58   4e-07
AFL467 (AFL467Q) /CSM/AF/AFL4-C/AFL467Q.Seq.d/                         58   4e-07
CHD879 (CHD879Q) /CSM/CH/CHD8-D/CHD879Q.Seq.d/                         50   9e-05
VHH517 (VHH517Q) /CSM/VH/VHH5-A/VHH517Q.Seq.d/                         38   0.33
VHF521 (VHF521Q) /CSM/VH/VHF5-A/VHF521Q.Seq.d/                         38   0.33
SSF256 (SSF256Q) /CSM/SS/SSF2-C/SSF256Q.Seq.d/                         38   0.33
SSC294 (SSC294Q) /CSM/SS/SSC2-D/SSC294Q.Seq.d/                         38   0.33
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ401096) Dictyostelium discoideum cDNA clone:dds22j06, 3' ...  1356   0.0    1
(BJ401941) Dictyostelium discoideum cDNA clone:dds19l20, 3' ...  1051   0.0    2
(BJ401767) Dictyostelium discoideum cDNA clone:dds19h07, 3' ...   373   0.0    4
(BJ390359) Dictyostelium discoideum cDNA clone:dds22j06, 5' ...   918   0.0    2
(BJ389741) Dictyostelium discoideum cDNA clone:dds19l20, 5' ...   852   0.0    2
(BJ345397) Dictyostelium discoideum cDNA clone:dda23f18, 3' ...    58   2e-10  4
(BJ401173) Dictyostelium discoideum cDNA clone:dds22k10, 3' ...    58   2e-10  4
(BJ376790) Dictyostelium discoideum cDNA clone:ddc29n20, 3' ...    50   1e-05  3
(AM457017) Vitis vinifera contig VV78X112955.9, whole genome...    54   0.014  1
(AC079308) Homo sapiens 3 BAC RP11-57P12 (Roswell Park Cance...    50   0.22   1
dna update 2009. 1.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JE8) RecName: Full=UPF0522 protein A; Flags: Precursor;           243   1e-62
AU279378_1(AU279378|pid:none) Cyprinus carpio BPI/LBP mRNA for b...    46   0.003
AB372877_1(AB372877|pid:none) Plecoglossus altivelis altivelis B...    44   0.013
FJ810853_1(FJ810853|pid:none) Sus scrofa bactericidal/permeabili...    43   0.021
EF436278_1(EF436278|pid:none) Sus scrofa bactericidal permeabili...    41   0.081
(O74346) RecName: Full=P cell-type agglutination protein map4; F...    41   0.081
AK315328_1(AK315328|pid:none) Homo sapiens cDNA, FLJ96367, highl...    41   0.11
AJ544235_2(AJ544235|pid:none) Shewanella frigidimarina omcA gene...    40   0.24
CP000447_2615(CP000447|pid:none) Shewanella frigidimarina NCIMB ...    40   0.24
AB042025_1(AB042025|pid:none) Oncorhynchus mykiss LBP/BPI-1 mRNA...    39   0.40
protein update 2009. 5.27
PSORT 

 psg: 0.98  gvh: 0.57  alm: 0.20  top: 0.20  tms: 0.07  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.42  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for SFK821 is end
5' end seq. ID SFK821F
5' end seq. 
>SFK821F.Seq
AATAATTATTCCTCATAATCCATGTATTTAAAAAAATAAAAAAATTATTCTTCATAATAC
ATGTATTTTAAAAAATAAAATATATATATATATAAATATATTTATTTATTCAAAATAAAA
TGAATAAAACTATCATACTTTTATTAATTTCAATTATTTTTGAAATAGTAATATCATCAT
CCGTTGATGGTAATTATCCAGCTGGAGGTTTATATTTTGGTGTATCATCAAATTTCTTGG
TTGGTGAATCTGAAAAGTTTACTCAATATGTTCAAGATGAATTATTAGCAATGGATTTGC
CAGATCAATCAGGTGATTCAGATGGAGTACAATATTCATTTACTCATTTTAAACTTGCAT
TAAATTTACAAGATTTCTTTTATGCACAATTAGGACCTGGTGTTTTTCAAATGGGATGGG
ATACTATTACATTTAATTTACAATGGGAATATGAAATTTGTGTTAAAAAAGTTGTAAAAC
TTTGTGAATCAGGCACAATTACAGTTTATACAGCAAGTGGTCAAAGTGTATCATTAGGAA
CTACATTAGATGTTTTATTTAATTCATCAAATGCAAAGATTGAGGCACCATCAACTGTTA
TGCCATTTGAGGCGG----------
Length of 5' end seq. 615
3' end seq. ID SFK821Z
3' end seq. 
>SFK821Z.Seq
----------AAACACCATCCTATGTACCATACGATTCACAAATGGAGTCTTTCACATCA
GACTACTGTATCACTATCACTGGTTACTTTATTGAGACCCTGTTGGATGCTGTATTGGTA
CCAGAGTTTCCAATGACAATTCAACCATCACAAATACCAGCTTCATCACCAGTTCAATTG
AATACAAGTTCTGATTTCTTTAGTGGTATCGCTCCAAATTTAACCTCTAAATATCCAAAT
GTTGGTATTCAAGTGAATTTAATGCCACCAACAATACCAACGGTTACGATCAATTCAAGT
GCTATCATTTTAACAGATTTTGAAATTTCAACTTCATTTTTAGTGTTAACTGATAATGAT
GATGCTATTCCTGTTTTCAATGTTTTGTTCAAATTTGATGCTGAAATCCAAACCACCCTT
TATACTGATAGTGTCTCTGTTTTCTCTTTAAATTCAACATTACTATCAATAACACCAAAT
GTAACCATAACTGATTCAAACGTTGGTTCTGTCGATGCCACTGGTTTTGTTCAACTAATT
GAAATGGCACAATCTATAATTAAAATTCCATCAATAACTTATCCTGTACCTTCAAAATAT
TCAATAACAAATGTAAATTCACAACTTGGTNATCAAATAATTCAACTTACTTTNATTTAA
TNAAAATTCAACNAATCAAAAATATCTTAAAAAATCTGTAAATTTA
Length of 3' end seq. 696
Connected seq. ID SFK821P
Connected seq. 
>SFK821P.Seq
AATAATTATTCCTCATAATCCATGTATTTAAAAAAATAAAAAAATTATTCTTCATAATAC
ATGTATTTTAAAAAATAAAATATATATATATATAAATATATTTATTTATTCAAAATAAAA
TGAATAAAACTATCATACTTTTATTAATTTCAATTATTTTTGAAATAGTAATATCATCAT
CCGTTGATGGTAATTATCCAGCTGGAGGTTTATATTTTGGTGTATCATCAAATTTCTTGG
TTGGTGAATCTGAAAAGTTTACTCAATATGTTCAAGATGAATTATTAGCAATGGATTTGC
CAGATCAATCAGGTGATTCAGATGGAGTACAATATTCATTTACTCATTTTAAACTTGCAT
TAAATTTACAAGATTTCTTTTATGCACAATTAGGACCTGGTGTTTTTCAAATGGGATGGG
ATACTATTACATTTAATTTACAATGGGAATATGAAATTTGTGTTAAAAAAGTTGTAAAAC
TTTGTGAATCAGGCACAATTACAGTTTATACAGCAAGTGGTCAAAGTGTATCATTAGGAA
CTACATTAGATGTTTTATTTAATTCATCAAATGCAAAGATTGAGGCACCATCAACTGTTA
TGCCATTTGAGGCGG----------AAACACCATCCTATGTACCATACGATTCACAAATG
GAGTCTTTCACATCAGACTACTGTATCACTATCACTGGTTACTTTATTGAGACCCTGTTG
GATGCTGTATTGGTACCAGAGTTTCCAATGACAATTCAACCATCACAAATACCAGCTTCA
TCACCAGTTCAATTGAATACAAGTTCTGATTTCTTTAGTGGTATCGCTCCAAATTTAACC
TCTAAATATCCAAATGTTGGTATTCAAGTGAATTTAATGCCACCAACAATACCAACGGTT
ACGATCAATTCAAGTGCTATCATTTTAACAGATTTTGAAATTTCAACTTCATTTTTAGTG
TTAACTGATAATGATGATGCTATTCCTGTTTTCAATGTTTTGTTCAAATTTGATGCTGAA
ATCCAAACCACCCTTTATACTGATAGTGTCTCTGTTTTCTCTTTAAATTCAACATTACTA
TCAATAACACCAAATGTAACCATAACTGATTCAAACGTTGGTTCTGTCGATGCCACTGGT
TTTGTTCAACTAATTGAAATGGCACAATCTATAATTAAAATTCCATCAATAACTTATCCT
GTACCTTCAAAATATTCAATAACAAATGTAAATTCACAACTTGGTNATCAAATAATTCAA
CTTACTTTNATTTAATNAAAATTCAACNAATCAAAAATATCTTAAAAAATCTGTAAATTT
A
Length of connected seq. 1311
Full length Seq ID -
Full length Seq. -
Length of full length seq. -