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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFK804
Library SF
(Link to library)
Clone ID SFK804
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID SFK804P
(Link to Original site)
Representative DNA sequence 
>SFK804 (SFK804Q) /CSM/SF/SFK8-A/SFK804Q.Seq.d/
ATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAA
TTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACA
CCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTG
GTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTG
TACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAG
TTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAG
GTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTG
CTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGAT
TACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGCAGGTAATAAATTAGCT
ATGCAAGAATTTATGATTTXXXXXXXXXXANAATTCATTAAANAANATCCNATCATTTCA
ATTNAANATCCNTTTGATCAANATGATTGGGAATCATACNCTNAATTAACAGCATCAGTT
GATATTCAAATTGTTGGTGATGATTTACTTGTTACCNACCCANAAAAAATTAANACTGGT
ATTNAAAAAAAAGCATGTAANGCTNTTTTATTAAAAGTTAACCNAATTGGNTCAGTCNCT
GAANCAATTNGNGCCGCTCTTGACNCAAANAATGCATCATGGGGNGTCATGGTTAGCCNT
CGNTCAGGTGAAACTGAANANACCNTCANTGCTGATTTAGNTGTTGGNTTAGGGACNGGN
CNAATTAAAACTGGNGCNCCATGTAGATCANAAAGATTAGC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhql
vfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqik
vn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*DYQFQHSMLSMGGSHAGNKLA
MQEFMI---

---XFIKXXPIISIXXPFDQXDWESYXXLTASVDIQIVGDDLLVTXPXKIXTGIXKKACX
AXLLKVNXIGSVXEXIXAALDXXNASWGVMVSXRSGETEXTXXADLXVGLGTGXIKTGAP
CRSXRL
Translated Amino Acid sequence (All Frames) 
Frame A:
iffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhql
vfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqik
vn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*DYQFQHSMLSMGGSHAGNKLA
MQEFMI---

---xih*xxsxhfnxxsx*sx*lgiixxinsis*ysncw**ftcyxpxkn*xwyxkksm*
xxfiks*pnwxsx*xnxxrs*xkxcimgxhg*psxr*n*xxxxc*fxcwxrdxxn*nwxx
m*ixkis

Frame B:
fffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssircinw
yl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk*r
*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwvvhmqvin*l
cknl*f---

---XFIKXXPIISIXXPFDQXDWESYXXLTASVDIQIVGDDLLVTXPXKIXTGIXKKACX
AXLLKVNXIGSVXEXIXAALDXXNASWGVMVSXRSGETEXTXXADLXVGLGTGXIKTGAP
CRSXRL

Frame C:
fflslsiyisffkmsviksikareildsrgnptvevdlytekdgvvssfraavpsgastg
iyeavelrdgdksrylgkgvlkaiknileviqpavigksvsdqaaidklmidldgtpnkg
klganailavslavcragaadrnlplykyiseiagtkmrlpvpafnvingwftcr**isy
ariyd---

---nslxxixsfqxxixlixmignhtln*qhqlifkllvmiyllptxkklxlvxkkkhvx
lfy*kltxlxqslxqxxplltqxmhhgxswlaxxqvklxxpsxli*xlx*gxxxlklxxh
vdxkd*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK804 (SFK804Q) /CSM/SF/SFK8-A/SFK804Q.Seq.d/                       1507   0.0
AFN685 (AFN685Q) /CSM/AF/AFN6-D/AFN685Q.Seq.d/                       1088   0.0
AFN159 (AFN159Q) /CSM/AF/AFN1-C/AFN159Q.Seq.d/                       1088   0.0
AFL547 (AFL547Q) /CSM/AF/AFL5-B/AFL547Q.Seq.d/                       1088   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1088   0.0
AFK532 (AFK532Q) /CSM/AF/AFK5-B/AFK532Q.Seq.d/                       1088   0.0
VFL491 (VFL491Q) /CSM/VF/VFL4-D/VFL491Q.Seq.d/                       1080   0.0
VFF123 (VFF123Q) /CSM/VF/VFF1-A/VFF123Q.Seq.d/                       1072   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1072   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1072   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ390346) Dictyostelium discoideum cDNA clone:dds22h02, 5' ...  1088   0.0    1
(BJ330150) Dictyostelium discoideum cDNA clone:dda27e15, 5' ...  1088   0.0    1
(BJ329281) Dictyostelium discoideum cDNA clone:dda24m11, 5' ...  1088   0.0    1
(BJ328620) Dictyostelium discoideum cDNA clone:dda28i22, 5' ...  1088   0.0    1
(BJ328050) Dictyostelium discoideum cDNA clone:dda22b22, 5' ...  1088   0.0    1
(BJ327975) Dictyostelium discoideum cDNA clone:dda22o07, 5' ...  1088   0.0    1
(BJ415809) Dictyostelium discoideum cDNA clone:ddv23f24, 5' ...  1080   0.0    1
(BJ412614) Dictyostelium discoideum cDNA clone:ddv9a10, 5' e...   993   0.0    2
(BJ411466) Dictyostelium discoideum cDNA clone:ddv4f14, 5' e...   993   0.0    2
(BJ410089) Dictyostelium discoideum cDNA clone:ddv11m05, 5' ...   993   0.0    2
dna update 2009. 1.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   300   2e-84
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   204   9e-56
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   204   1e-55
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   206   2e-55
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   203   3e-55
(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   203   6e-55
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   201   8e-55
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   201   8e-55
CR859195_1(CR859195|pid:none) Pongo abelii mRNA; cDNA DKFZp459B1...   201   8e-55
BC017249_1(BC017249|pid:none) Homo sapiens enolase 3 (beta, musc...   201   1e-54
protein update 2009. 5.27
PSORT 

 psg: 0.75  gvh: 0.51  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SFK804 is cyt
5' end seq. ID SFK804F
5' end seq. 
>SFK804F.Seq
ATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAA
TTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACA
CCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTG
GTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTG
TACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAG
TTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAG
GTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTG
CTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGAT
TACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGCAGGTAATAAATTAGCT
ATGCAAGAATTTATGATTT----------
Length of 5' end seq. 559
3' end seq. ID SFK804Z
3' end seq. 
>SFK804Z.Seq
----------ANAATTCATTAAANAANATCCNATCATTTCAATTNAANATCCNTTTGATC
AANATGATTGGGAATCATACNCTNAATTAACAGCATCAGTTGATATTCAAATTGTTGGTG
ATGATTTACTTGTTACCNACCCANAAAAAATTAANACTGGTATTNAAAAAAAAGCATGTA
ANGCTNTTTTATTAAAAGTTAACCNAATTGGNTCAGTCNCTGAANCAATTNGNGCCGCTC
TTGACNCAAANAATGCATCATGGGGNGTCATGGTTAGCCNTCGNTCAGGTGAAACTGAAN
ANACCNTCANTGCTGATTTAGNTGTTGGNTTAGGGACNGGNCNAATTAAAACTGGNGCNC
CATGTAGATCANAAAGATTAGC
Length of 3' end seq. 372
Connected seq. ID SFK804P
Connected seq. 
>SFK804P.Seq
ATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAA
TTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACA
CCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTG
GTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTG
TACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAG
TTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAG
GTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTG
CTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGAT
TACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGCAGGTAATAAATTAGCT
ATGCAAGAATTTATGATTT----------ANAATTCATTAAANAANATCCNATCATTTCA
ATTNAANATCCNTTTGATCAANATGATTGGGAATCATACNCTNAATTAACAGCATCAGTT
GATATTCAAATTGTTGGTGATGATTTACTTGTTACCNACCCANAAAAAATTAANACTGGT
ATTNAAAAAAAAGCATGTAANGCTNTTTTATTAAAAGTTAACCNAATTGGNTCAGTCNCT
GAANCAATTNGNGCCGCTCTTGACNCAAANAATGCATCATGGGGNGTCATGGTTAGCCNT
CGNTCAGGTGAAACTGAANANACCNTCANTGCTGATTTAGNTGTTGGNTTAGGGACNGGN
CNAATTAAAACTGGNGCNCCATGTAGATCANAAAGATTAGC
Length of connected seq. 931
Full length Seq ID -
Full length Seq. -
Length of full length seq. -