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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI637
Library AF
(Link to library)
Clone ID AFI637
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15617-1
Original site URL
Representative seq. ID AFI637P
(Link to Original site)
Representative DNA sequence 
>AFI637 (AFI637Q) /CSM/AF/AFI6-B/AFI637Q.Seq.d/
ATTATATTTTATATTATTATTTTTATTATTATTATTTTATTACTAACTTAATATCATATT
AAGTTTACATTAAAAATTTAAAAAATAAATAAAAAAATAAAAAATTAAATTAAAAAAAAA
TAAAAAAAATAAAAAAAAAGAAAAAAAAANTAAAAANCTAATANTTAAAAAAATTAANTN
TTGTGGTAAAAAAATAAATAAATAAATAAATAANTAAATAANTAAATAANTAAATAANCA
AATAANTAAATAANTAANTAAATAANTAAXXXXXXXXXXAAATCATTCAAAGCAAAGGTC
ATGGTAAAGCGGTCGATTGGTGGGCACTTGGTATTCTAATATTTGAAATGTTGGCAGGGT
TATCCACCTTTNTATGATGATGATACCTTTGCAATCTATAATAAAATTTTGGCTGGTAGA
ATCACTTTTCCATTAGGTTTTGATGTTGATGCTAAAGATTTAATTAAAAGATTATTAACT
GCTGATAGAACTCGTAGATTGGGTGCTTTAAAGGATGGTGCATTAGATGTCAAAAATCAT
AGATGGTTTTCTGATATTAATTGGGAAAGATTATATCAGAGAAGAGATAATGGACCATTC
ATTCCAAAGATTCAACATCAAGGTGATTCTTCAAACTTTGAAATGTATGATGAAGAAGAA
ATGGTTGAAGAGCCTCCATCTTCAAATTATGTTGATCCATATGCACATCTTNTCAAGGAT
TTTTAAAAAATAAAAGATAATAACAAATAAAACAAATTATAATAATTAAAAATAATAATA
ATAAAAGTGTCAATTAAAAATAACAAAAATAATAATAATAATAATAATAATAATAATAAT
AATAATAATAATAATAATAATAATAATAATAATAATATATGATAAACTAATATGGTTTGT
GTCAATATCTACAAATAAATAAGTTTTCATATCATAATAACACCAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
yilyyyfyyyyfitnlisy*vyiknlknk*knkkln*kkikkikkkkkkxkx*xlkklxx
vvkk*ink*IXk*xnx*ixk*xnx*xnx*---

---IIQSKGHGKAVDWWALGILIFEMLAGLSTFX***ylcnl**nfgw*nhfsirf*c*c
*rfn*kiinc**ns*igcfkgwcircqks*mvf*y*lgkiisekr*wtihskdstsr*ff
kl*nv**rrng*rasifklc*sictsxqgflknkr**qikqiiiiknnnnksvn*k*qk*
*********************ymin*yglcqylqinkfsyhnnt
Translated Amino Acid sequence (All Frames) 
Frame A:
iifyiiifiiiilllt*yhikftlki*kinkkikn*ikkk*kk*kkrkkx*kxnx*kn*x
lw*knk*ink*xnx*IXk*xnx*ixx*ix---

---ksfkakvmvkrsigghlvf*ylkcwqgyppxydddtfaiynkilagritfplgfdvd
akdlikrlltadrtrrlgalkdgaldvknhrwfsdinwerlyqrrdngpfipkiqhqgds
snfemydeeemveeppssnyvdpyahlxkdf*kikdnnk*nkl**lkiiiikvsiknnkn
nnnnnnnnnnnnnnnnnnnnnni**tnmvcvniyk*isfhiiitp

Frame B:
lyfillfllllfyy*lniilslh*kfkk*ikk*kiklkknkknkkkekkxkxlIXkkixx
cgkkink*inx*ixk*xnxqixk*xxk*x---

---nhskqrsw*sgrlvgtwysni*nvgrvihlxmmmiplqsiikfwlveslfh*vlmlm
lki*lkdy*llielvdwvl*rmvh*mskiidgfliligkdyireeimdhsfqrfnikvil
qtlkcmmkkkwlkslhlqimlihmhixsrifkk*kiitnktnynn*k****kcqlkitki
iiiiiiiiiiiiiiiiiiiiiiydkliwfvsistnk*vfis**hq

Frame C:
yilyyyfyyyyfitnlisy*vyiknlknk*knkkln*kkikkikkkkkkxkx*xlkklxx
vvkk*ink*IXk*xnx*ixk*xnx*xnx*---

---IIQSKGHGKAVDWWALGILIFEMLAGLSTFX***ylcnl**nfgw*nhfsirf*c*c
*rfn*kiinc**ns*igcfkgwcircqks*mvf*y*lgkiisekr*wtihskdstsr*ff
kl*nv**rrng*rasifklc*sictsxqgflknkr**qikqiiiiknnnnksvn*k*qk*
*********************ymin*yglcqylqinkfsyhnnt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI637 (AFI637Q) /CSM/AF/AFI6-B/AFI637Q.Seq.d/                        866   0.0
SFH701 (SFH701Q) /CSM/SF/SFH7-A/SFH701Q.Seq.d/                        850   0.0
CHP121 (CHP121Q) /CSM/CH/CHP1-A/CHP121Q.Seq.d/                        844   0.0
CHL103 (CHL103Q) /CSM/CH/CHL1-A/CHL103Q.Seq.d/                        844   0.0
CHI186 (CHI186Q) /CSM/CH/CHI1-D/CHI186Q.Seq.d/                        839   0.0
AHM265 (AHM265Q) /CSM/AH/AHM2-C/AHM265Q.Seq.d/                        839   0.0
CHH347 (CHH347Q) /CSM/CH/CHH3-B/CHH347Q.Seq.d/                        809   0.0
CHM664 (CHM664Q) /CSM/CH/CHM6-C/CHM664Q.Seq.d/                        799   0.0
CHK312 (CHK312Q) /CSM/CH/CHK3-A/CHK312Q.Seq.d/                        644   0.0
CHJ469 (CHJ469Q) /CSM/CH/CHJ4-C/CHJ469Q.Seq.d/                        628   e-179
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M38703|M38703.1 Dictyostelium discoideum protein kinase (Dd GPK2) gene, exons 1 and 2.   412   0.0    4
CR354088|CR354088.1 Gallus gallus finished cDNA, clone ChEST371o8.    70   7e-08  1
BU401108|BU401108.1 603482232F1 CSEQCHN59 Gallus gallus cDNA clone ChEST371o8 5', mRNA sequence.    60   7e-05  1
CD167015|CD167015.1 MM1-0008P-R014-B11-U.B MM1-0008 Schistosoma mansoni cDNA clone MM1-0008P-R014-B11.B, mRNA sequence.    50   0.066  1
AP004173|AP004173.1 Mycoplasma penetrans HF-2 DNA, complete genome, section 4/5.    32   0.092  12
AA581319|AA581319.1 nd43b09.r1 NCI_CGAP_AR1 Homo sapiens cDNA clone IMAGE:803033 5', mRNA sequence.    34   0.15   3
AQ635628|AQ635628.1 RPCI-11-480H19.TJ RPCI-11 Homo sapiens genomic clone RPCI-11-480H19, genomic survey sequence.    34   0.19   3
CA801489|CA801489.1 sau06d12.y2 Gm-c1062 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1062-7464 5' similar to TR:P93489 P93489 PROTEIN KINASE HOMOLOG PSPK4. ;, mRNA sequence.    48   0.26   1
BM886851|BM886851.1 sam31a06.y1 Gm-c1068 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1068-6179 5' similar to TR:P93489 P93489 PROTEIN KINASE HOMOLOG PSPK4. ;, mRNA sequence.    48   0.26   1
BQ741249|BQ741249.1 saq16b06.y1 Gm-c1045 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1045-4692 5' similar to TR:P93489 P93489 PROTEIN KINASE HOMOLOG PSPK4. ;, mRNA sequence.    48   0.26   1
dna update 2004. 9.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

JQ1150(JQ1150)protein kinase (EC 2.7.1.37) cAMP-dependent, catal...   223   6e-68
(P34099) RecName: Full=cAMP-dependent protein kinase catalytic s...   223   6e-68
BX322647_6(BX322647|pid:none) Zebrafish DNA sequence from clone ...   119   2e-36
F31751(F31751) protein kinase catalytic chain homolog DC2 - frui...   111   4e-33
AE014296_2746(AE014296|pid:none) Drosophila melanogaster chromos...   111   4e-33
Y12638_2(Y12638|pid:none) Trypanosoma brucei brucei brucei AnTat...   109   2e-31
AJ297564_1(AJ297564|pid:none) Leishmania major (Friedlin) PKAC2a...   107   3e-31
AM494971_399(AM494971|pid:none) Leishmania braziliensis chromoso...   107   3e-31
CP000081_398(CP000081|pid:none) Leishmania major chromosome 35, ...   107   3e-31
A84453_1(A84453|pid:none) Sequence 68 from Patent WO9845704.  &A...   109   6e-31
protein update 2009. 4.23
PSORT 

 psg: 0.80  gvh: 0.49  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.11  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: Golgi

>> prediction for AFI637 is cyt
5' end seq. ID AFI637F
5' end seq. 
>AFI637F.Seq
ATTATATTTTATATTATTATTTTTATTATTATTATTTTATTACTAACTTAATATCATATT
AAGTTTACATTAAAAATTTAAAAAATAAATAAAAAAATAAAAAATTAAATTAAAAAAAAA
TAAAAAAAATAAAAAAAAAGAAAAAAAAANTAAAAANCTAATANTTAAAAAAATTAANTN
TTGTGGTAAAAAAATAAATAAATAAATAAATAANTAAATAANTAAATAANTAAATAANCA
AATAANTAAATAANTAANTAAATAANTAA----------
Length of 5' end seq. 269
3' end seq. ID AFI637Z
3' end seq. 
>AFI637Z.Seq
----------AAATCATTCAAAGCAAAGGTCATGGTAAAGCGGTCGATTGGTGGGCACTT
GGTATTCTAATATTTGAAATGTTGGCAGGGTTATCCACCTTTNTATGATGATGATACCTT
TGCAATCTATAATAAAATTTTGGCTGGTAGAATCACTTTTCCATTAGGTTTTGATGTTGA
TGCTAAAGATTTAATTAAAAGATTATTAACTGCTGATAGAACTCGTAGATTGGGTGCTTT
AAAGGATGGTGCATTAGATGTCAAAAATCATAGATGGTTTTCTGATATTAATTGGGAAAG
ATTATATCAGAGAAGAGATAATGGACCATTCATTCCAAAGATTCAACATCAAGGTGATTC
TTCAAACTTTGAAATGTATGATGAAGAAGAAATGGTTGAAGAGCCTCCATCTTCAAATTA
TGTTGATCCATATGCACATCTTNTCAAGGATTTTTAAAAAATAAAAGATAATAACAAATA
AAACAAATTATAATAATTAAAAATAATAATAATAAAAGTGTCAATTAAAAATAACAAAAA
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAA
TAATAATATATGATAAACTAATATGGTTTGTGTCAATATCTACAAATAAATAAGTTTTCA
TATCATAATAACACCAA
Length of 3' end seq. 667
Connected seq. ID AFI637P
Connected seq. 
>AFI637P.Seq
ATTATATTTTATATTATTATTTTTATTATTATTATTTTATTACTAACTTAATATCATATT
AAGTTTACATTAAAAATTTAAAAAATAAATAAAAAAATAAAAAATTAAATTAAAAAAAAA
TAAAAAAAATAAAAAAAAAGAAAAAAAAANTAAAAANCTAATANTTAAAAAAATTAANTN
TTGTGGTAAAAAAATAAATAAATAAATAAATAANTAAATAANTAAATAANTAAATAANCA
AATAANTAAATAANTAANTAAATAANTAA----------AAATCATTCAAAGCAAAGGTC
ATGGTAAAGCGGTCGATTGGTGGGCACTTGGTATTCTAATATTTGAAATGTTGGCAGGGT
TATCCACCTTTNTATGATGATGATACCTTTGCAATCTATAATAAAATTTTGGCTGGTAGA
ATCACTTTTCCATTAGGTTTTGATGTTGATGCTAAAGATTTAATTAAAAGATTATTAACT
GCTGATAGAACTCGTAGATTGGGTGCTTTAAAGGATGGTGCATTAGATGTCAAAAATCAT
AGATGGTTTTCTGATATTAATTGGGAAAGATTATATCAGAGAAGAGATAATGGACCATTC
ATTCCAAAGATTCAACATCAAGGTGATTCTTCAAACTTTGAAATGTATGATGAAGAAGAA
ATGGTTGAAGAGCCTCCATCTTCAAATTATGTTGATCCATATGCACATCTTNTCAAGGAT
TTTTAAAAAATAAAAGATAATAACAAATAAAACAAATTATAATAATTAAAAATAATAATA
ATAAAAGTGTCAATTAAAAATAACAAAAATAATAATAATAATAATAATAATAATAATAAT
AATAATAATAATAATAATAATAATAATAATAATAATATATGATAAACTAATATGGTTTGT
GTCAATATCTACAAATAAATAAGTTTTCATATCATAATAACACCAA
Length of connected seq. 936
Full length Seq ID -
Full length Seq. -
Length of full length seq. -