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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFK591
Library SF
(Link to library)
Clone ID SFK591
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFK591P
(Link to Original site)
Representative DNA sequence 
>SFK591 (SFK591Q) /CSM/SF/SFK5-D/SFK591Q.Seq.d/
ATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTT
TATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCA
AAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAG
ATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAA
AAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTT
TCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTG
ATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATG
GTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTC
ACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTG
AAACCGATCAATTAACTCATCAATACACTTTXXXXXXXXXXAGAGATCCAAGCAGGTAAC
CTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAAA
CCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGT
CATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAAT
GAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAA
TGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATT
GATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATC
GGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGAC
TCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAA
AAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAA
AAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAA
GAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCA
ACCAAAAAAGTGATCANACGAACTTTAAANAAATAAATAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYT---

---EIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDP
EAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNEL
YLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAK
AEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVIXRTLXK
*i
Translated Amino Acid sequence (All Frames) 
Frame A:
ifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikk
mvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkl
ivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstkl
kpin*lintl---

---rdpsr*pr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*s
rscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*t
lpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrq
s*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n**qkrrsqkinqksdxtnfkx
ink

Frame B:
yl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rr
w*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*
lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*
nrsinssihf---

---EIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDP
EAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNEL
YLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAK
AEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVIXRTLXK
*i

Frame C:
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYT---

---rskqvtslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliq
klsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmns
tslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppk
lkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklmtkkkksknqpkk*sxel*xn
k*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK591 (SFK591Q) /CSM/SF/SFK5-D/SFK591Q.Seq.d/                       2161   0.0
VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                       1132   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                       1132   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                       1132   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                       1132   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                       1132   0.0
VFH220 (VFH220Q) /CSM/VF/VFH2-A/VFH220Q.Seq.d/                       1132   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                       1132   0.0
VFH121 (VFH121Q) /CSM/VF/VFH1-A/VFH121Q.Seq.d/                       1132   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                       1132   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U36937) Dictyostelium discoideum calreticulin mRNA, complet...  1049   0.0    1
(BJ433933) Dictyostelium discoideum cDNA clone:ddv23k07, 3' ...  1049   0.0    1
(BJ432219) Dictyostelium discoideum cDNA clone:ddv18e06, 3' ...  1049   0.0    1
(BJ431077) Dictyostelium discoideum cDNA clone:ddv9e22, 3' e...  1049   0.0    1
(BJ430883) Dictyostelium discoideum cDNA clone:ddv9k05, 3' e...  1049   0.0    1
(BJ401298) Dictyostelium discoideum cDNA clone:dds22e23, 3' ...  1049   0.0    1
(BJ400978) Dictyostelium discoideum cDNA clone:dds15m17, 3' ...  1049   0.0    1
(BJ400775) Dictyostelium discoideum cDNA clone:dds14b20, 3' ...  1049   0.0    1
(BJ377902) Dictyostelium discoideum cDNA clone:ddc26i09, 3' ...  1049   0.0    1
(BJ347672) Dictyostelium discoideum cDNA clone:dda27f21, 3' ...  1049   0.0    1
dna update 2009. 1.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   329   0.0
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   204   e-103
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   206   e-103
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   207   e-103
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   200   e-103
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   201   e-103
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   204   e-102
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   204   e-102
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   197   e-101
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   202   e-101
protein update 2009. 5.27
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for SFK591 is exc
5' end seq. ID SFK591F
5' end seq. 
>SFK591F.Seq
ATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTT
TATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCA
AAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAG
ATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAA
AAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTT
TCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTG
ATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATG
GTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTC
ACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTG
AAACCGATCAATTAACTCATCAATACACTTT----------
Length of 5' end seq. 571
3' end seq. ID SFK591Z
3' end seq. 
>SFK591Z.Seq
----------AGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAA
AACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCG
ATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATC
CAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAA
CCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAAT
ACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAAC
TCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTT
CCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAA
AGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCA
AAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAG
AAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTG
ATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTGATCANACGAACTTTAAANA
AATAAATAAA
Length of 3' end seq. 720
Connected seq. ID SFK591P
Connected seq. 
>SFK591P.Seq
ATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTT
TATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCA
AAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAG
ATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAA
AAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTT
TCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTG
ATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATG
GTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTC
ACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTG
AAACCGATCAATTAACTCATCAATACACTTT----------AGAGATCCAAGCAGGTAAC
CTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAAA
CCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGT
CATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAAT
GAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAA
TGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATT
GATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATC
GGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGAC
TCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAA
AAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAA
AAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAA
GAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCA
ACCAAAAAAGTGATCANACGAACTTTAAANAAATAAATAAA
Length of connected seq. 1291
Full length Seq ID -
Full length Seq. -
Length of full length seq. -