>AFI610 (AFI610Q) /CSM/AF/AFI6-A/AFI610Q.Seq.d/
ATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTAAC
CACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAATGG
TCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAACG
TATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTTAC
CTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGAAC
ACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAAAG
AGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTCGG
TAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCATAC
ACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTAGG
TCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCAAA
ATCATATCGTCATATGCATGGTXXXXXXXXXXTTCCATTCAATTGATGGAATTTGAAGAT
GCCTTAAAGTATCGTTTCAATCCATTCGATGTAACAAAGATTTGGTCACACAAAGACTAT
CCATTAATTCAAATTGGTCGTATGGTTTTGAATCGTAATCCAGAGAATTACTTTGCTGAA
GTTGAACAAGCTGCCTTCTCACCATCCCACATGGTTCCAGGTATTGAACCATCACCAGAT
AAGATGTTACAAGGTCGTCTCTTCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTA
AACTATCAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTAC
CAACGTGATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCA
AACTCTTTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAACATAAATTTGACGTCTCT
GGTTTCGCTGCTCGTCAACCATACAATCATCCAAATGACGACTTTGTTCAACCAGGTGAT
TTATATAGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCAC
ATGTCCGGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCT
GACAAAGATTTAGGTGCTCGTTTATGTAAGGGTCTGGTATTTATGTTAANAGTCATCAAA
TTCGCGCAGATCAAACT

yfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRER
IPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKGSSDSER
DPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFLG
QTPESTHQVSILFSDRGTPKSYRHMHG---

---SIQLMEFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLNRNPENYFAEVEQAAFSP
SHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQRDGFMAV
NGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLYRLMSED
AKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLVFMLXVIKFAQIK

Frame A:
ifylgkink*inkyvsssfnhq*wftn**qfklndrrc*wsnfnsrfhfn**tcsfrs*t
yprtcctc*rcwcpwlfrsylk*ctkmv*s*ilk*sw*entnlhpflycww*krfirf*k
rskrfrcqily*rr*l*ygr**yssflys*sikiprfhsytkt*stnkl*rskyvlgffr
sntrintssinfil**rytkiissyaw---

---fhsidgi*rclkvsfqsircnkdlvtqrlsinsnwsygfes*srellc*s*tscllt
iphgsry*titr*dvtrsslllsryssssfrcklstnpsqlsirrkgwc*klpt*wfhgc
qw*ww*rsklstklfrwsratsricst*i*rlwfrcsstiqssk*rlcstr*fi*thv*r
cqisfrl*fsrshvrchhqrnssscrfkllqg*qrfrcsfm*gsgiyvxshqiradqt

Frame B:
yfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRER
IPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKGSSDSER
DPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFLG
QTPESTHQVSILFSDRGTPKSYRHMHG---

---SIQLMEFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLNRNPENYFAEVEQAAFSP
SHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQRDGFMAV
NGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLYRLMSED
AKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLVFMLXVIKFAQIK

Frame C:
ilfgknk*ink*icqlqf*ppvvvhqliti*tq*pqvlmvqf*fkisl*linllisivnv
sqnvlymlkvlvpmvisklpqvmyqngvklns*iklvrehqsspvslllvvkkvhpilke
iqevslsnfilkkvtliwsviilqfslfvihqnsqisfihknvihkqivkiqicfgif*v
khqnqhikyqfyslievhqnhivicm---

---pfn*wnlkmp*sivsihsm*qrfghtktih*fklvvwf*iviqritllklnklpshh
ptwfqvlnhhqircykvvssliqiliviv*v*tinksqsivhsp*rvvlkttnvmvswls
mvmvvkvqtinqtlsvvqshiqnllninltslvsllvnhtiiqmttlfnqviyidsclkm
pnlvssli*svtcpvspskkfkfvpfqtftrltki*vlvyvrvwylc*xssnsrrsn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI610 (AFI610Q) /CSM/AF/AFI6-A/AFI610Q.Seq.d/                       2504   0.0
AFO835 (AFO835Q) /CSM/AF/AFO8-B/AFO835Q.Seq.d/                       1362   0.0
SFL238 (SFL238Q) /CSM/SF/SFL2-B/SFL238Q.Seq.d/                       1354   0.0
AFD795 (AFD795Q) /CSM/AF/AFD7-D/AFD795Q.Seq.d/                       1348   0.0
AFK218 (AFK218Q) /CSM/AF/AFK2-A/AFK218Q.Seq.d/                       1342   0.0
AFE376 (AFE376Q) /CSM/AF/AFE3-D/AFE376Q.Seq.d/                       1334   0.0
CFI634 (CFI634Q) /CSM/CF/CFI6-B/CFI634Q.Seq.d/                       1332   0.0
CFI568 (CFI568Q) /CSM/CF/CFI5-C/CFI568Q.Seq.d/                       1332   0.0
CFA669 (CFA669Q) /CSM/CF/CFA6-C/CFA669Q.Seq.d/                       1332   0.0
AFH524 (AFH524Q) /CSM/AF/AFH5-A/AFH524Q.Seq.d/                       1332   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF090443|AF090443.1 Dictyostelium discoideum catalase (cat) mRNA, complete cds.  1314   0.0    2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1314   0.0    13
AB064338|AB064338.1 Candida boidinii CTA1 gene for peroxisomal catalase, complete cds.   111   1e-64  7
E65314|E65314.1 Novel catalase, gene of said catalase and composition containing  said  catalase, and process for preparing catalase using the genetic engineering  technology.    86   9e-33  4
AP004595|AP004595.1 Oceanobacillus iheyensis HTE831 genomic DNA, section 3/13.    72   2e-31  9
AX536932|AX536932.1 Sequence 533 from Patent WO02064766.          101   7e-29  3
AR375090|AR375090.1 Sequence 96 from patent US 6605709.            76   7e-28  3
AJ400965|AJ400965.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, strain PR.    76   4e-27  3
AJ400964|AJ400964.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, wild type.    76   4e-27  3
AB006327|AB006327.1 Candida albicans mRNA for catalase, complete cds.    94   2e-26  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF090443_1(AF090443|pid:none) Dictyostelium discoideum catalase ...   469   e-130
AY378080_1(AY378080|pid:none) Tetrahymena thermophila putative c...   313   8e-84
(O93662) RecName: Full=Catalase;          EC=1.11.1.6;  &AJ00593...   309   1e-82
CP000300_481(CP000300|pid:none) Methanococcoides burtonii DSM 62...   301   3e-80
(Q9XZD5) RecName: Full=Peroxisomal catalase;          EC=1.11.1....   294   4e-78
CQ796333_1(CQ796333|pid:none) Sequence 109 from Patent WO2004027...   292   2e-77
AE017333_3935(AE017333|pid:none) Bacillus licheniformis DSM 13, ...   292   2e-77
CP000560_826(CP000560|pid:none) Bacillus amyloliquefaciens FZB42...   288   3e-76
(P26901) RecName: Full=Vegetative catalase;          EC=1.11.1.6...   287   6e-76
(P90682) RecName: Full=Catalase;          EC=1.11.1.6;  &Y10611_...   285   2e-75

 psg: 0.89  gvh: 0.57  alm: 0.34  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for AFI610 is cyt

>AFI610F.Seq
ATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTAAC
CACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAATGG
TCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAACG
TATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTTAC
CTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGAAC
ACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAAAG
AGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTCGG
TAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCATAC
ACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTAGG
TCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCAAA
ATCATATCGTCATATGCATGGT----------

>AFI610Z.Seq
----------TTCCATTCAATTGATGGAATTTGAAGATGCCTTAAAGTATCGTTTCAATC
CATTCGATGTAACAAAGATTTGGTCACACAAAGACTATCCATTAATTCAAATTGGTCGTA
TGGTTTTGAATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCAC
CATCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCT
TCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCA
ATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTG
TCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGC
CACATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCAT
ACAATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAG
ATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAG
AAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTT
TATGTAAGGGTCTGGTATTTATGTTAANAGTCATCAAATTCGCGCAGATCAAACT

>AFI610P.Seq
ATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTAAC
CACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAATGG
TCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAACG
TATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTTAC
CTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGAAC
ACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAAAG
AGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTCGG
TAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCATAC
ACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTAGG
TCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCAAA
ATCATATCGTCATATGCATGGT----------TTCCATTCAATTGATGGAATTTGAAGAT
GCCTTAAAGTATCGTTTCAATCCATTCGATGTAACAAAGATTTGGTCACACAAAGACTAT
CCATTAATTCAAATTGGTCGTATGGTTTTGAATCGTAATCCAGAGAATTACTTTGCTGAA
GTTGAACAAGCTGCCTTCTCACCATCCCACATGGTTCCAGGTATTGAACCATCACCAGAT
AAGATGTTACAAGGTCGTCTCTTCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTA
AACTATCAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTAC
CAACGTGATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCA
AACTCTTTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAACATAAATTTGACGTCTCT
GGTTTCGCTGCTCGTCAACCATACAATCATCCAAATGACGACTTTGTTCAACCAGGTGAT
TTATATAGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCAC
ATGTCCGGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCT
GACAAAGATTTAGGTGCTCGTTTATGTAAGGGTCTGGTATTTATGTTAANAGTCATCAAA
TTCGCGCAGATCAAACT