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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFK294
Library SF
(Link to library)
Clone ID SFK294
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16528-1
Original site URL
Representative seq. ID SFK294F
(Link to Original site)
Representative DNA sequence 
>SFK294 (SFK294Q) /CSM/SF/SFK2-D/SFK294Q.Seq.d/
AATAAAAAATTTTCAAAATATTTAGTGTATTCTGACAATTAATTATTGTTTCAGTACAAT
TTAAAAAAGATGAAATTAAATCCAAACGCTTCATCGTTTGTACCAAAATTTGTTGCTAAA
CCAGCAGCACCAGCACCAGCAGCACCAGTTGCTGTTGTTGCCGAACCAGTAGCAGCACCA
GTTGTTGCAGAACCAACACCAACACCAGCACCAGTAGAAACTAAAGAAGAACCAACTACC
ACAGCTACTACAACTTCAGTATCATCAATTAAAACTGAAGAAGATTTAGAAAATATTAAA
CCAACTGAATCAGTATATAAAGTTGAAGATGATGACATCGAAGATGATGATGAAGTTGAT
GAAGTTGCAGAGAAAATTGAACAAGTTGTAAAAGTATTACCAGAAGATAGCAGAGAACAT
CTTAATATAGTATTTTTAGGACATGTAGATGCAGGTAAATCAACACTTTCAGGTAGTATT
ATGGTTTTAACAGGACAAGTTGATCCACATACTCTTGCAAAATATGAAAGAGAAGCCAAA
GAGAACCATCGTGAAGGTTGGATCTATGCATACATTATGGATACAAACGAAGAAGAACGT
ACCAAAGGTAAGACAGTAGAGGTTGGGTCGTGCACATTTTGAAXXXXXXXXXX
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nkkfskylvysdn*LLFQYNLKKMKLNPNASSFVPKFVAKPAAPAPAAPVAVVAEPVAAP
VVAEPTPTPAPVETKEEPTTTATTTSVSSIKTEEDLENIKPTESVYKVEDDDIEDDDEVD
EVAEKIEQVVKVLPEDSREHLNIVFLGHVDAGKSTLSGSIMVLTGQVDPHTLAKYEREAK
ENHREGWIYAYIMDTNEEERTKGKTVEVGSCTF*---
Translated Amino Acid sequence (All Frames) 
Frame A:
nkkfskylvysdn*LLFQYNLKKMKLNPNASSFVPKFVAKPAAPAPAAPVAVVAEPVAAP
VVAEPTPTPAPVETKEEPTTTATTTSVSSIKTEEDLENIKPTESVYKVEDDDIEDDDEVD
EVAEKIEQVVKVLPEDSREHLNIVFLGHVDAGKSTLSGSIMVLTGQVDPHTLAKYEREAK
ENHREGWIYAYIMDTNEEERTKGKTVEVGSCTF*---


Frame B:
iknfqni*ciltinycfsti*kr*n*iqtlhrlyqnlllnqqhqhqqhqllllpnq*qhq
llqnqhqhqhq*klkknqlpqllqlqyhqlklkki*kilnqlnqyiklkmmtskmmmklm
klqrklnkl*kyyqkiaenili*yf*dm*mqvnqhfqvvlwf*qdklihillqnmkekpk
rtivkvgsmhtlwiqtkknvpkvrq*rlgrahf---


Frame C:
*kifkifsvf*qliivsvqfkkdeikskrfivctkicc*tsststsstsccccrtsssts
ccrtntntstsrn*rrtnyhsyynfsiin*n*rrfrky*tn*isi*s*r**hrr***s**
scren*tscksitrr*qrts*ysifrtcrcr*intfr*yygfnrts*styscki*krsqr
eps*rldlcihygykrrrtyqr*dsrgwvvhil---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK294 (SFK294Q) /CSM/SF/SFK2-D/SFK294Q.Seq.d/                       1275   0.0
AFI501 (AFI501Q) /CSM/AF/AFI5-A/AFI501Q.Seq.d/                       1259   0.0
AFH445 (AFH445Q) /CSM/AF/AFH4-B/AFH445Q.Seq.d/                       1259   0.0
VFL286 (VFL286Q) /CSM/VF/VFL2-D/VFL286Q.Seq.d/                       1251   0.0
AFB363 (AFB363Q) /CSM/AF/AFB3-C/AFB363Q.Seq.d/                       1251   0.0
AFA743 (AFA743Q) /CSM/AF/AFA7-B/AFA743Q.Seq.d/                       1251   0.0
AFA544 (AFA544Q) /CSM/AF/AFA5-B/AFA544Q.Seq.d/                       1251   0.0
AFO174 (AFO174Q) /CSM/AF/AFO1-D/AFO174Q.Seq.d/                       1243   0.0
AFE348 (AFE348Q) /CSM/AF/AFE3-B/AFE348Q.Seq.d/                       1235   0.0
AFH761 (AFH761Q) /CSM/AF/AFH7-C/AFH761Q.Seq.d/                       1231   0.0
own update 2001.11.27
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ390291) Dictyostelium discoideum cDNA clone:dds21k24, 5' ...   910   0.0    2
(BJ415831) Dictyostelium discoideum cDNA clone:ddv23k22, 5' ...   876   0.0    3
(BJ410435) Dictyostelium discoideum cDNA clone:ddv12d17, 5' ...   876   0.0    3
(BJ326775) Dictyostelium discoideum cDNA clone:dda18a01, 5' ...   876   0.0    3
(BJ326477) Dictyostelium discoideum cDNA clone:dda16j15, 5' ...   876   0.0    3
(BJ326136) Dictyostelium discoideum cDNA clone:dda15j12, 5' ...   876   0.0    3
(BJ325798) Dictyostelium discoideum cDNA clone:dda2g11, 5' e...   876   0.0    3
(BJ325795) Dictyostelium discoideum cDNA clone:dda2f11, 5' e...   876   0.0    3
(BJ325057) Dictyostelium discoideum cDNA clone:dda9p11, 5' e...   876   0.0    3
(BJ328845) Dictyostelium discoideum cDNA clone:dda29c19, 5' ...   868   0.0    3
dna update 2008.12.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q7YZN9) RecName: Full=Eukaryotic peptide chain release factor G...   201   2e-50
DQ489316_1(DQ489316|pid:none) Triticum aestivum GTP-binding prot...   113   5e-24
AL663014_10(AL663014|pid:none) Oryza sativa genomic DNA, chromos...   112   9e-24
AP008210_354(AP008210|pid:none) Oryza sativa (japonica cultivar-...   112   9e-24
CR940353_470(CR940353|pid:none) Theileria annulata strain Ankara...   110   3e-23
AC034107_8(AC034107|pid:none) Sequence of BAC T10F20 from Arabid...   110   3e-23
AE014186_226(AE014186|pid:none) Plasmodium falciparum 3D7 chromo...   110   3e-23
AM910991_208(AM910991|pid:none) Plasmodium knowlesi strain H chr...   110   3e-23
BC053244_1(BC053244|pid:none) Danio rerio G1 to S phase transiti...   109   6e-23
L37045_1(L37045|pid:none) Xenopus laevis (clone XLSUP35) SUP35 m...   109   6e-23
protein update 2009. 5.27
PSORT 

 psg: 0.79  gvh: 0.54  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for SFK294 is nuc
5' end seq. ID SFK294F
5' end seq. 
>SFK294F.Seq
AATAAAAAATTTTCAAAATATTTAGTGTATTCTGACAATTAATTATTGTTTCAGTACAAT
TTAAAAAAGATGAAATTAAATCCAAACGCTTCATCGTTTGTACCAAAATTTGTTGCTAAA
CCAGCAGCACCAGCACCAGCAGCACCAGTTGCTGTTGTTGCCGAACCAGTAGCAGCACCA
GTTGTTGCAGAACCAACACCAACACCAGCACCAGTAGAAACTAAAGAAGAACCAACTACC
ACAGCTACTACAACTTCAGTATCATCAATTAAAACTGAAGAAGATTTAGAAAATATTAAA
CCAACTGAATCAGTATATAAAGTTGAAGATGATGACATCGAAGATGATGATGAAGTTGAT
GAAGTTGCAGAGAAAATTGAACAAGTTGTAAAAGTATTACCAGAAGATAGCAGAGAACAT
CTTAATATAGTATTTTTAGGACATGTAGATGCAGGTAAATCAACACTTTCAGGTAGTATT
ATGGTTTTAACAGGACAAGTTGATCCACATACTCTTGCAAAATATGAAAGAGAAGCCAAA
GAGAACCATCGTGAAGGTTGGATCTATGCATACATTATGGATACAAACGAAGAAGAACGT
ACCAAAGGTAAGACAGTAGAGGTTGGGTCGTGCACATTTTGAA----------
Length of 5' end seq. 643
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -