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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFK136
Library SF
(Link to library)
Clone ID SFK136
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID SFK136F
(Link to Original site)
Representative DNA sequence 
>SFK136 (SFK136Q) /CSM/SF/SFK1-B/SFK136Q.Seq.d/
ATTATTTTTTTATACATTAATTTTTTTTTAAAAAAAAATTATTTAATTTAAATTAACAAA
GATTTTGGCGCACAGATTTCAACAAAGATTTTTTTATTAATCAATTCCACATGATTCAAT
AAAGGGGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTTAGCTCA
AGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCC
ACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGA
TTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAA
AGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCAC
TGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGC
CAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCA
AGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGCCAXXXX
XXXXXX
sequence update 2001. 6. 1
Translated Amino Acid sequence 
lffytliff*kkii*fkltkilahrfqqrffy*SIPHDSIKGKMKLLILTLFLATIVLAQ
ALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTIPISDFEDAQYYGAITIGTPGQAFK
VVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASSTYVANGTDFTIQYGSGAMSGFVSQ
DSVTVGSLTVKDQLFAEA---
Translated Amino Acid sequence (All Frames) 
Frame A:
iiflyinfflkknyli*inkdfgaqistkifllinst*fnkgeneityfnfifsyycfss
sfnstiklpssfkri*kksstkmvkqiicsqcwyhnpnfrf*rcsilwchyhwyprsslq
ssfrywfiqlvdsikemsnhcccm*ft*qi*qrclkhicrqrn*fhhpir*wcyvrfcls
rfrhcwfinc*rsiir*s---


Frame B:
lffytliff*kkii*fkltkilahrfqqrffy*SIPHDSIKGKMKLLILTLFLATIVLAQ
ALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTIPISDFEDAQYYGAITIGTPGQAFK
VVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASSTYVANGTDFTIQYGSGAMSGFVSQ
DSVTVGSLTVKDQLFAEA---


Frame C:
yffih*fffkkklfnln*qrfwrtdfnkdffinqfhmiq*rgk*nylf*lyf*lllf*lk
l*qyh*tsiklqenleeefhkngqtdyllsmlvpqsqfqilkmlntmvplplvpqvkpsk
*fsilvhptcgfhqrnvqslllhviyitnitavpqahmsptelispsntvvvlcqvlslk
ipsllvh*llkinyslk---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK136 (SFK136Q) /CSM/SF/SFK1-B/SFK136Q.Seq.d/                       1031   0.0
SFG678 (SFG678Q) /CSM/SF/SFG6-D/SFG678Q.Seq.d/                       1031   0.0
SFF752 (SFF752Q) /CSM/SF/SFF7-C/SFF752Q.Seq.d/                       1031   0.0
SFE532 (SFE532Q) /CSM/SF/SFE5-B/SFE532Q.Seq.d/                       1031   0.0
SFB665 (SFB665Q) /CSM/SF/SFB6-C/SFB665Q.Seq.d/                       1031   0.0
SFB424 (SFB424Q) /CSM/SF/SFB4-A/SFB424Q.Seq.d/                       1031   0.0
SFA510 (SFA510Q) /CSM/SF/SFA5-A/SFA510Q.Seq.d/                       1031   0.0
CHD888 (CHD888Q) /CSM/CH/CHD8-D/CHD888Q.Seq.d/                       1031   0.0
CHD726 (CHD726Q) /CSM/CH/CHD7-B/CHD726Q.Seq.d/                       1031   0.0
CHA784 (CHA784Q) /CSM/CH/CHA7-D/CHA784Q.Seq.d/                       1031   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ358942) Dictyostelium discoideum cDNA clone:ddc12d04, 5' ...   993   0.0    2
(BJ360201) Dictyostelium discoideum cDNA clone:ddc4n22, 5' e...   993   0.0    2
(BJ386738) Dictyostelium discoideum cDNA clone:dds10o07, 5' ...   993   0.0    2
(BJ325945) Dictyostelium discoideum cDNA clone:dda3j02, 5' e...   993   0.0    2
(BJ387536) Dictyostelium discoideum cDNA clone:dds3p06, 5' e...   993   0.0    2
(BJ359585) Dictyostelium discoideum cDNA clone:ddc15p12, 5' ...   993   0.0    2
(BJ387722) Dictyostelium discoideum cDNA clone:dds4a18, 5' e...   993   0.0    2
(BJ328991) Dictyostelium discoideum cDNA clone:dda30i12, 5' ...   993   0.0    2
(BJ390150) Dictyostelium discoideum cDNA clone:dds21g09, 5' ...   993   0.0    2
(BJ359112) Dictyostelium discoideum cDNA clone:ddc12h22, 5' ...   993   0.0    2
dna update 2008.12.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   155   8e-37
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   153   3e-36
AF454831_1(AF454831|pid:none) Apriona germari cathepsin D mRNA, ...   153   3e-36
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   152   9e-36
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   150   2e-35
(O93428) RecName: Full=Cathepsin D;          EC=3.4.23.5; Flags:...   148   1e-34
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   148   1e-34
BT043515_1(BT043515|pid:none) Salmo salar clone HM4_0887 catheps...   146   5e-34
CP001577_36(CP001577|pid:none) Micromonas sp. RCC299 chromosome ...   145   6e-34
EF193385_1(EF193385|pid:none) Musca domestica aspartic proteinas...   145   6e-34
protein update 2009. 5.27
PSORT 

 psg: 0.86  gvh: 0.77  alm: 0.47  top: 0.60  tms: 0.00  mit: 0.32  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 24.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: nuclear

>> prediction for SFK136 is exc
5' end seq. ID SFK136F
5' end seq. 
>SFK136F.Seq
ATTATTTTTTTATACATTAATTTTTTTTTAAAAAAAAATTATTTAATTTAAATTAACAAA
GATTTTGGCGCACAGATTTCAACAAAGATTTTTTTATTAATCAATTCCACATGATTCAAT
AAAGGGGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTTAGCTCA
AGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCC
ACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGA
TTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAA
AGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCAC
TGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGC
CAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCA
AGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGCCA----
------
Length of 5' end seq. 596
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -