>SFJ731 (SFJ731Q) /CSM/SF/SFJ7-B/SFJ731Q.Seq.d/
ATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAG
AGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGG
AATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATT
AAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGT
AAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGA
TGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAAT
CGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTA
CCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAA
TGTTATTAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGT
ACCCATTGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTC
ACTCAAAGAGATXXXXXXXXXXGATGCTATCAATCATTCGGGCTATCAAGATGTTATTAA
AATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATAT
GGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATT
AATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATT
TGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCA
AATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGA
GAAAACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTAT
TGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGG
TGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAA
ATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGA
AGAAGAACTTGAAAAACTTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGNAAGAG
TTTTATCATTTTTAATAAAAATTAAAAAAAAA

flif**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFEL
RDGDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAI
VGVSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLV
PIGAPKFKEALRYGSEVYHSLKE---

---DAINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSGEQLMSRY
LEILKKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKAISEKTVN
SLLLKINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGLGTGEIKSGAP
CRFERLSKYNRLMKIEEELEKLHRSNGNSFEYAGKSFIIFNKN*kk

Frame A:
ifniliiiilkney*in*s*rnfg**r*snirs*smdk*rnvfsqstkwsinwys*si*i
krw**kslfwiwcfecnc*ck*fnitnieryes**sksnr**nd*igwyke*iktwvqcn
rwcingsgkssrfy*rcalvplhftigwk**ysftstmf*cy*wwktcr**ttisrvyas
thwctki*rsialwfrslpftqrd---

---DAINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSGEQLMSRY
LEILKKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKAISEKTVN
SLLLKINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGLGTGEIKSGAP
CRFERLSKYNRLMKIEEELEKLHRSNGNSFEYAGKSFIIFNKN*kk

Frame B:
flif**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFEL
RDGDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAI
VGVSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLV
PIGAPKFKEALRYGSEVYHSLKE---

---mlsiiraikmllklvlilllqnfgiqrlqnmiwisri*dphi*slv*ivvsn*cldi
*rf*ksiqlhslkihlvkmigkimvklqlqlvikfkslvmiyfvqiqkelkkqflrkl*i
hyy*k*iksvh*lkllklqnlqkklvgvv*lvivqvklmiisllil*lvlvlvk*nlvhl
vdlkdyqniid**klkknlknfidqmvihlsmlxrvlsflikikkk

Frame C:
f*yfnnynfkk*vlnqlklekfwiveviqh*klkygqvkecfqpkyqvehqlvfmkhln*
emvikvvildmvf*mqllm*ii*yhqh*kv*eliikkq*mik*lnwmvqrinqnlgpmqs
lvyqwqwqeqplllkvcpctvtfhnwleivifvyqyhvlmllmvenmqvidyhfkslc*y
plvhqnlkkhcvmvqkstihskr---

---cyqsfglsrcy*nwy*fccfrileskdfki*ygfqeyriltfev*fk*w*ainv*if
rdfkkvsncil*rsiw*r*lgksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enckf
iiienksnrytn*ny*scktckrswlglfs*ssfr*n**sfhc*scswswyw*nkiwctl
*i*kiiki**inen*rrt*kts*ikw*fi*vcwxefyhf**klkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFJ731 (SFJ731Q) /CSM/SF/SFJ7-B/SFJ731Q.Seq.d/                       2478   0.0
CHB680 (CHB680Q) /CSM/CH/CHB6-D/CHB680Q.Seq.d/                       1289   0.0
CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/                       1283   0.0
CHB459 (CHB459Q) /CSM/CH/CHB4-C/CHB459Q.Seq.d/                       1271   0.0
CHC156 (CHC156Q) /CSM/CH/CHC1-C/CHC156Q.Seq.d/                       1265   0.0
CFH624 (CFH624Q) /CSM/CF/CFH6-A/CFH624Q.Seq.d/                       1265   0.0
CFK240 (CFK240Q) /CSM/CF/CFK2-B/CFK240Q.Seq.d/                       1261   0.0
CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/                       1251   0.0
CFG491 (CFG491Q) /CSM/CF/CFG4-D/CFG491Q.Seq.d/                       1223   0.0
CHD848 (CHD848Q) /CSM/CH/CHD8-B/CHD848Q.Seq.d/                       1221   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    56   1e-26  5
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   7e-19  6
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   7e-19  6
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   7e-19  6
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   7e-19  6
BM162118|BM162118.1 EST564641 PyBS Plasmodium yoelii yoelii cDNA clone PYCKN41 5' end, mRNA sequence.    70   5e-18  3
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    70   6e-18  3
BM164058|BM164058.1 EST566581 PyBS Plasmodium yoelii yoelii cDNA clone PYCLP21 5' end, mRNA sequence.    70   7e-16  2
BM166362|BM166362.1 EST568885 PyBS Plasmodium yoelii yoelii cDNA clone PYCNG69 5' end, mRNA sequence.    70   1e-15  2
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           58   4e-15  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   439   e-122
(Q27727) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   437   e-121
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   435   e-120
BC054169_1(BC054169|pid:none) Xenopus laevis enolase 1, alpha, m...   435   e-120
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   435   e-120
(P17183) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   434   e-120
(P07323) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   434   e-120
AM910990_161(AM910990|pid:none) Plasmodium knowlesi strain H chr...   434   e-120
(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   434   e-120
JC1039(JC1039)phosphopyruvate hydratase (EC 4.2.1.11) - rat           433   e-120

 psg: 0.56  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SFJ731 is cyt

>SFJ731F.Seq
ATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAG
AGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGG
AATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATT
AAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGT
AAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGA
TGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAAT
CGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTA
CCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAA
TGTTATTAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGT
ACCCATTGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTC
ACTCAAAGAGAT----------

>SFJ731Z.Seq
----------GATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAATTGGTATTGA
TTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAA
TATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAATGTCTAGATA
TTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGA
TTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGA
TGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAA
TTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAA
ACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGA
TCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATCTGGTGCACC
TTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACTTGA
AAAACTTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGNAAGAGTTTTATCATTTT
TAATAAAAATTAAAAAAAAA

>SFJ731P.Seq
ATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAG
AGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGG
AATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATT
AAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGT
AAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGA
TGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAAT
CGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTA
CCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAA
TGTTATTAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGT
ACCCATTGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTC
ACTCAAAGAGAT----------GATGCTATCAATCATTCGGGCTATCAAGATGTTATTAA
AATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATAT
GGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATT
AATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATT
TGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCA
AATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGA
GAAAACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTAT
TGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGG
TGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAA
ATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGA
AGAAGAACTTGAAAAACTTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGNAAGAG
TTTTATCATTTTTAATAAAAATTAAAAAAAAA