>AFI546 (AFI546Q) /CSM/AF/AFI5-B/AFI546Q.Seq.d/
XXXXXXXXXXCNCCNCCNCAACCNCGACCACAACAACAACAATTACCTCAACCAACTCAA
TTAAATAATCCACCACAACCACCAATTTCTTTAAGTAAAAATGATAGTAGTAATAGTATA
AATAGTAATAGTAATGGTGAAATTGTAAGAGCACTTTATGATTATAATGCAACAGAGGAG
AATGAAATTTCATTTAAAGCAAATGCTTTAATTAAAGTTGTTTTACGTGACGAAAGTGGT
TGGTGGCAAGGTATGGTAATTGGAGAGAGTGATCGTATTGGTGTTTTCCCAAGTAATTTT
ATTTCAGATTCTTCTGATTCTTCAAAGAAAAGAGTGGATGTAGCAGGTAGAAAATGTAAA
GTACTTTACGATTATCGTACAGATTGTGAGGGTGAATTAAATATTAAAGAAGGTGAAATT
TTAACAATTGAATATCCACATGAAGGTTGGCCCTTTGGTTCAAAT

---PPQPRPQQQQLPQPTQLNNPPQPPISLSKNDSSNSINSNSNGEIVRALYDYNATEEN
EISFKANALIKVVLRDESGWWQGMVIGESDRIGVFPSNFISDSSDSSKKRVDVAGRKCKV
LYDYRTDCEGELNIKEGEILTIEYPHEGWPFGSN

Frame A:
---xxxtxtttttitstnsik*sttttnffk*k*****yk****w*nckstl*l*cnrge
*nfi*skcfn*scft*rkwlvarygnwre*sywcfpk*fyfrff*ffkeksgcsr*km*s
tlrlsyrl*g*iky*rr*nfnn*ist*rlalwfk


Frame B:
---PPQPRPQQQQLPQPTQLNNPPQPPISLSKNDSSNSINSNSNGEIVRALYDYNATEEN
EISFKANALIKVVLRDESGWWQGMVIGESDRIGVFPSNFISDSSDSSKKRVDVAGRKCKV
LYDYRTDCEGELNIKEGEILTIEYPHEGWPFGSN


Frame C:
---xxnxdhnnnnylnqln*iihhnhqfl*vkmivviv*ivivmvkl*ehfmiimqqrrm
kfhlkqml*lklfyvtkvvggkvw*lervivlvfsqvilfqillilqrkewm*qvenvky
ftiivqivrvn*ilkkvkf*qlnihmkvgplvq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI546 (AFI546Q) /CSM/AF/AFI5-B/AFI546Q.Seq.d/                        880   0.0
AFH802 (AFH802Q) /CSM/AF/AFH8-A/AFH802Q.Seq.d/                        819   0.0
AFJ893 (AFJ893Q) /CSM/AF/AFJ8-D/AFJ893Q.Seq.d/                        803   0.0
SLE682 (SLE682Q) /CSM/SL/SLE6-D/SLE682Q.Seq.d/                        775   0.0
CFB209 (CFB209Q) /CSM/CF/CFB2-A/CFB209Q.Seq.d/                        731   0.0
AFK574 (AFK574Q) /CSM/AF/AFK5-D/AFK574Q.Seq.d/                        728   0.0
SSA528 (SSA528Q) /CSM/SS/SSA5-B/SSA528Q.Seq.d/                        638   0.0
SSK482 (SSK482Q) /CSM/SS/SSK4-D/SSK482Q.Seq.d/                         48   1e-04
VSF667 (VSF667Q) /CSM/VS/VSF6-C/VSF667Q.Seq.d/                         46   5e-04
SFE436 (SFE436Q) /CSM/SF/SFE4-B/SFE436Q.Seq.d/                         46   5e-04

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.   833   0.0    9
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    50   9e-09  13
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    34   1e-07  10
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    42   3e-07  10
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    34   1e-06  12
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    34   4e-06  11
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    40   1e-05  10
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    32   2e-05  9
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    44   7e-05  14
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    42   1e-04  13

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC085613_1(BC085613|pid:none) Homo sapiens SH3 domain containing...    60   2e-08
AK092528_1(AK092528|pid:none) Homo sapiens cDNA FLJ35209 fis, cl...    60   2e-08
AL133047_1(AL133047|pid:none) Homo sapiens mRNA; cDNA DKFZp434D0...    60   2e-08
BX647422_1(BX647422|pid:none) Homo sapiens mRNA; cDNA DKFZp686I0...    60   2e-08
AK294476_1(AK294476|pid:none) Homo sapiens cDNA FLJ61692 complet...    60   2e-08
AK291025_1(AK291025|pid:none) Homo sapiens cDNA FLJ76174 complet...    60   2e-08
BC108893_1(BC108893|pid:none) Homo sapiens SH3 domain containing...    60   2e-08
BC108890_1(BC108890|pid:none) Homo sapiens SH3 domain containing...    60   2e-08
AB168387_1(AB168387|pid:none) Macaca fascicularis testis cDNA cl...    59   4e-08
BC046842_1(BC046842|pid:none) Xenopus laevis myosin I e, mRNA (c...    57   2e-07

 psg: 0.78  gvh: 0.26  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for AFI546 is nuc

>AFI546Z.Seq
----------CNCCNCCNCAACCNCGACCACAACAACAACAATTACCTCAACCAACTCAA
TTAAATAATCCACCACAACCACCAATTTCTTTAAGTAAAAATGATAGTAGTAATAGTATA
AATAGTAATAGTAATGGTGAAATTGTAAGAGCACTTTATGATTATAATGCAACAGAGGAG
AATGAAATTTCATTTAAAGCAAATGCTTTAATTAAAGTTGTTTTACGTGACGAAAGTGGT
TGGTGGCAAGGTATGGTAATTGGAGAGAGTGATCGTATTGGTGTTTTCCCAAGTAATTTT
ATTTCAGATTCTTCTGATTCTTCAAAGAAAAGAGTGGATGTAGCAGGTAGAAAATGTAAA
GTACTTTACGATTATCGTACAGATTGTGAGGGTGAATTAAATATTAAAGAAGGTGAAATT
TTAACAATTGAATATCCACATGAAGGTTGGCCCTTTGGTTCAAAT