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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFJ301
Library SF
(Link to library)
Clone ID SFJ301
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFJ301Z
(Link to Original site)
Representative DNA sequence 
>SFJ301 (SFJ301Q) /CSM/SF/SFJ3-A/SFJ301Q.Seq.d/
XXXXXXXXXXAGATCCAAGCAGGTACCCTCGCTGATGATTGGGAATTACTTCCATCAAAA
CAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGAT
GACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCA
GAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACC
ATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATAC
AAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTC
TACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCC
ATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAG
ACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAA
GCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAA
GCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTANAACTGAG
ACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTGATCAGACGAACTTTAAANNAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---IQAGTLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPE
AVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELY
LFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKA
EERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVXTETKKKKSKNQPKK*sdel*x
Translated Amino Acid sequence (All Frames) 
Frame A:
---dpsrypr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*sr
scqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tl
pl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrqs
*rekes*rkiscrksc*krsrrs**rrrrsr*rrfsxn*dkkeevkkstkkvirrtlxx


Frame B:
---IQAGTLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPE
AVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELY
LFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKA
EERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVXTETKKKKSKNQPKK*sdel*x


Frame C:
---skqvpslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliqk
lsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmnst
slmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppkl
krerkltkn*lpkkllkkkpkklmkkkkkslkki*lxlrqkrrsqkinqksdqtnfkx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                       1059   0.0
SFJ301 (SFJ301Q) /CSM/SF/SFJ3-A/SFJ301Q.Seq.d/                       1059   0.0
AFN849 (AFN849Q) /CSM/AF/AFN8-C/AFN849Q.Seq.d/                       1059   0.0
AFN318 (AFN318Q) /CSM/AF/AFN3-A/AFN318Q.Seq.d/                       1059   0.0
AFI321 (AFI321Q) /CSM/AF/AFI3-A/AFI321Q.Seq.d/                       1059   0.0
AFE885 (AFE885Q) /CSM/AF/AFE8-D/AFE885Q.Seq.d/                       1059   0.0
AFD172 (AFD172Q) /CSM/AF/AFD1-C/AFD172Q.Seq.d/                       1059   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                       1057   0.0
SFI129 (SFI129Q) /CSM/SF/SFI1-B/SFI129Q.Seq.d/                       1053   0.0
SFA732 (SFA732Q) /CSM/SF/SFA7-B/SFA732Q.Seq.d/                       1053   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1051   0.0    2
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-15  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   5e-11  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   3e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74   3e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74   3e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   3e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   3e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74   3e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74   3e-09  1
dna update 2004. 1. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   323   4e-87
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   202   8e-51
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   199   5e-50
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   197   2e-49
AK230218_1(AK230218|pid:none) Arabidopsis thaliana mRNA for hypo...   196   6e-49
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   195   1e-48
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   195   1e-48
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   193   4e-48
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   193   4e-48
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   192   6e-48
protein update 2009. 5.26
PSORT 

 psg: 0.75  gvh: 0.25  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SFJ301 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SFJ301Z
3' end seq. 
>SFJ301Z.Seq
----------AGATCCAAGCAGGTACCCTCGCTGATGATTGGGAATTACTTCCATCAAAA
CAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGAT
GACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCA
GAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACC
ATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATAC
AAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTC
TACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCC
ATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAG
ACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAA
GCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAA
GCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTANAACTGAG
ACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTGATCAGACGAACTTTAAANNAA
Length of 3' end seq. 709
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -