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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFJ161
Library SF
(Link to library)
Clone ID SFJ161
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09860-1
Original site URL
Representative seq. ID SFJ161P
(Link to Original site)
Representative DNA sequence 
>SFJ161 (SFJ161Q) /CSM/SF/SFJ1-C/SFJ161Q.Seq.d/
ATTTTTAAGCATTATAATTAATACACATTTTGTTTTTTTTCCTCTTTTTTATTCTTTTAT
TTTCATTTTATTTTCCATTGTAAATATATTTAGTAAAAAATAAAATAAAATAAAATAGAA
TAATAAATAAAATAAAAAAGAATGTCATCTCCACCAACAACAACAACAACAACCACCACT
TCAACAGGCGGCAACCAAGAAACACCAAAAGCAATACCTCCAGCACCAATTGAAGATAAA
TCAGCTGAAAGAATAAAGAAAAGAGATCCAGGTAATATTACAAAGGTTATTAAATGTGGT
GTTGTAGGTGATGGTACTGTTGGAAAAACAACACTCTTATTATCTTATATCACTCAAGCA
TTCATTACAGAGTATACACCAACTGTATTTGATAACTTTTCAGCAATTGAAGAGGTTGAT
GATGGTAAGTTAGTAAATGTTATATTGTGGGATACTGCTGGTCAAGATGACTATGCACAA
ATCAGAACAACTTGTTATACAAATTGTAGATATGATGTATTTTTACTTTGTTTCTCTGTT
GTAAATCGTGATTCATTTGACAATGTTAAATATAAGTXXXXXXXXXXTATTTTAGTTGGT
ACAAAAACTGATATGAGAGATCAACAAACAGGAGGTTCACCAACCAATACTACATCATCA
CCACCAATTGCAGGTAATAATCAAACATTAAATAATATCATTACATTTAAAGAGGGTCAA
AAGAGAGCCAAAGAGATTAAAGCATTCAACTATATGGAATGTACATCTCGTGACTCTTCA
AGTGTTGCCAAGGTAGTTTTGGAGGCAATCAATATCATCATTGAAAAGGAAAAATTAAAA
AGAGTAAACAATGAAAAACTATGGAAGAGTCAAGTGAAAAAAGAAGAGAAAGAATCTAAA
AAAGAGGAAAAAGAAAGAGCAAAATATATTGAAAAACAAAAATTAAAAGAAGAAAAATCG
AATGGTTCAGTAAATAATAGTAATAATAATAGTAATAGTAACAATAATAATAGTAATAAT
AATAATAATAATAGTAATAGTAGTAGTAATAATAATATAAGTGGAAATAAAGACAATAAT
AATTCAACACCAAATAAATCATTATCTTCCTCCTCATCTTCTGATAAAAAACAACAACCT
TCATCACCTTCCTCTGCTGCCTCTCCTTCGACACCTTCTTCACCTTCTCCCTTAGAAGGC
ACTTCTCCAAAACAAGATAAAAAAATTAACGCNCATATTAAATAGTAAAATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ifkhyn*ytfcffssflffyfhfifhckyi**kik*nkie**ik*KRMSSPPTTTTTTTT
STGGNQETPKAIPPAPIEDKSAERIKKRDPGNITKVIKCGVVGDGTVGKTTLLLSYITQA
FITEYTPTVFDNFSAIEEVDDGKLVNVILWDTAGQDDYAQIRTTCYTNCRYDVFLLCFSV
VNRDSFDNVKYK---

---ILVGTKTDMRDQQTGGSPTNTTSSPPIAGNNQTLNNIITFKEGQKRAKEIKAFNYME
CTSRDSSSVAKVVLEAINIIIEKEKLKRVNNEKLWKSQVKKEEKESKKEEKERAKYIEKQ
KLKEEKSNGSVNNSNNNSNSNNNNSNNNNNNSNSSSNNNISGNKDNNNSTPNKSLSSSSS
SDKKQQPSSPSSAASPSTPSSPSPLEGTSPKQDKKINAHIK**n
Translated Amino Acid sequence (All Frames) 
Frame A:
ifkhyn*ytfcffssflffyfhfifhckyi**kik*nkie**ik*KRMSSPPTTTTTTTT
STGGNQETPKAIPPAPIEDKSAERIKKRDPGNITKVIKCGVVGDGTVGKTTLLLSYITQA
FITEYTPTVFDNFSAIEEVDDGKLVNVILWDTAGQDDYAQIRTTCYTNCRYDVFLLCFSV
VNRDSFDNVKYK---

---yfswykn*yerstnrrftnqyyiittncr**snik*yhyi*rgskesqrd*siqlyg
myis*lfkccqgsfggnqyhh*kgkikkskq*ktmeessekrreri*krgkrkskiy*kt
kikrrkiewfsk*********q*****************ykwk*rq**fntk*iiiflli
f**ktttfitflcclsfdtfftfslrrhfsktr*kn*rxy*ivk

Frame B:
flsiiinthfvffplfysfifilfsivnifskk*nkik*nnk*nkkechlhqqqqqqppl
qqaatkkhqkqylqhqlkinqlke*rkeiqvilqrllnvvl*vmvllekqhsyylislkh
slqsihqlylitfqqlkrlmmvs**mlycgillvkmtmhkseqlviqivdmmyfyfvsll
*ivihltmlnis---

---ILVGTKTDMRDQQTGGSPTNTTSSPPIAGNNQTLNNIITFKEGQKRAKEIKAFNYME
CTSRDSSSVAKVVLEAINIIIEKEKLKRVNNEKLWKSQVKKEEKESKKEEKERAKYIEKQ
KLKEEKSNGSVNNSNNNSNSNNNNSNNNNNNSNSSSNNNISGNKDNNNSTPNKSLSSSSS
SDKKQQPSSPSSAASPSTPSSPSPLEGTSPKQDKKINAHIK**n

Frame C:
f*al*lihilffflffillfsfyfpl*iylvknkik*nriinkikknvistnnnnnnhhf
nrrqprntksntsstn*r*is*knkekrsr*yykgy*mwccr*wycwknntliilyhssi
hyrvytnci**lfsn*rg**w*vskcyivgycwsr*lctnqnnllykl*i*ciftlflcc
ks*fi*qc*i*---

---f*lvqkli*einkqevhqpilhhhhqlqviikh*iislhlkrvkrepkrlkhstiwn
vhlvtlqvlpr*fwrqsisslkrkn*ke*tmknygrvk*kkkrknlkkrkkkeqnilknk
n*kkknrmvq*iiviiivivtiiiviiiiiivivvviii*veiktiiiqhqinhylpphl
liknnnlhhlpllpllrhllhllp*kallqnkikkltxilnski
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFJ161 (SFJ161Q) /CSM/SF/SFJ1-C/SFJ161Q.Seq.d/                       1225   0.0
VFH820 (VFH820Q) /CSM/VF/VFH8-A/VFH820Q.Seq.d/                        801   0.0
AFA514 (AFA514Q) /CSM/AF/AFA5-A/AFA514Q.Seq.d/                        801   0.0
CFI357 (CFI357Q) /CSM/CF/CFI3-C/CFI357Q.Seq.d/                        793   0.0
AFM444 (AFM444Q) /CSM/AF/AFM4-B/AFM444Q.Seq.d/                        785   0.0
VSF750 (VSF750Q) /CSM/VS/VSF7-C/VSF750Q.Seq.d/                        268   2e-70
VSI802 (VSI802Q) /CSM/VS/VSI8-A/VSI802Q.Seq.d/                         48   3e-04
VSE610 (VSE610Q) /CSM/VS/VSE6-A/VSE610Q.Seq.d/                         48   3e-04
VSC436 (VSC436Q) /CSM/VS/VSC4-B/VSC436Q.Seq.d/                         48   3e-04
SSM636 (SSM636Q) /CSM/SS/SSM6-B/SSM636Q.Seq.d/                         48   3e-04
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L11594|L11594.1 Slime mold RacD protein mRNA, complete cds.        42   4e-15  4
L11592|L11592.1 Slime mold RacB protein mRNA, complete cds.        48   5e-15  4
AF153328|AF153328.1 Dictyostelium discoideum Rac1C (rac1C) mRNA, complete cds.    46   7e-12  3
AF310885|AF310885.1 Dictyostelium discoideum Rac1C (rac1C) gene, complete cds; and unknown gene.    46   2e-10  3
AF037042|AF037042.2 Dictyostelium discoideum ras-related GTPase RacF1 (racF1) gene, complete cds; tRNA-Glu gene, complete sequence; and unknown genes.    52   3e-10  4
AF310889|AF310889.1 Dictyostelium discoideum RacD (racD) gene, complete cds; and unknown genes.    42   2e-07  3
AF310892|AF310892.1 Dictyostelium discoideum citrate synthase (gltA) gene, partial cds; RacF2 (racF2) gene, complete cds; and unknown gene.    44   1e-06  5
AU289848|AU289848.1 Zinnia elegans cDNA, clone:Z4387, primer:M13 forward, sequence 1.    42   1e-06  3
AZ549190|AZ549190.1 ENTFF35TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38   1e-05  4
L11590|L11590.1 Slime mold Rac1C protein mRNA, 3' end.             46   2e-05  2
dna update 2004. 1. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q24817) RecName: Full=Rho-related protein racD; Flags: Precurso...   115   4e-24
(P34148) RecName: Full=Rho-related protein racB; Flags: Precurso...   114   5e-24
AF310887_1(AF310887|pid:none) Dictyostelium discoideum RacB (rac...   114   5e-24
AY438585_1(AY438585|pid:none) Fucus distichus Rho family GTPase ...   112   2e-23
DQ081716_1(DQ081716|pid:none) Paracoccidioides brasiliensis Rac1...   112   3e-23
AY540629_1(AY540629|pid:none) Aspergillus niger RacA gene, compl...   111   6e-23
L11594_1(L11594|pid:none) Slime mold RacD protein mRNA, complete...   110   1e-22
AF126054_1(AF126054|pid:none) Zea mays Rop3 small GTP binding pr...   109   2e-22
CS559854_1(CS559854|pid:none) Sequence 441 from Patent WO2006032...   109   2e-22
AC122722_29(AC122722|pid:none) Medicago truncatula clone mth1-3f...   109   2e-22
protein update 2009. 5.26
PSORT 

 psg: 0.85  gvh: 0.35  alm: 0.41  top: 0.27  tms: 0.00  mit: 0.47  mip: 0.02
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SFJ161 is mit
5' end seq. ID SFJ161F
5' end seq. 
>SFJ161F.Seq
ATTTTTAAGCATTATAATTAATACACATTTTGTTTTTTTTCCTCTTTTTTATTCTTTTAT
TTTCATTTTATTTTCCATTGTAAATATATTTAGTAAAAAATAAAATAAAATAAAATAGAA
TAATAAATAAAATAAAAAAGAATGTCATCTCCACCAACAACAACAACAACAACCACCACT
TCAACAGGCGGCAACCAAGAAACACCAAAAGCAATACCTCCAGCACCAATTGAAGATAAA
TCAGCTGAAAGAATAAAGAAAAGAGATCCAGGTAATATTACAAAGGTTATTAAATGTGGT
GTTGTAGGTGATGGTACTGTTGGAAAAACAACACTCTTATTATCTTATATCACTCAAGCA
TTCATTACAGAGTATACACCAACTGTATTTGATAACTTTTCAGCAATTGAAGAGGTTGAT
GATGGTAAGTTAGTAAATGTTATATTGTGGGATACTGCTGGTCAAGATGACTATGCACAA
ATCAGAACAACTTGTTATACAAATTGTAGATATGATGTATTTTTACTTTGTTTCTCTGTT
GTAAATCGTGATTCATTTGACAATGTTAAATATAAGT----------
Length of 5' end seq. 577
3' end seq. ID SFJ161Z
3' end seq. 
>SFJ161Z.Seq
----------TATTTTAGTTGGTACAAAAACTGATATGAGAGATCAACAAACAGGAGGTT
CACCAACCAATACTACATCATCACCACCAATTGCAGGTAATAATCAAACATTAAATAATA
TCATTACATTTAAAGAGGGTCAAAAGAGAGCCAAAGAGATTAAAGCATTCAACTATATGG
AATGTACATCTCGTGACTCTTCAAGTGTTGCCAAGGTAGTTTTGGAGGCAATCAATATCA
TCATTGAAAAGGAAAAATTAAAAAGAGTAAACAATGAAAAACTATGGAAGAGTCAAGTGA
AAAAAGAAGAGAAAGAATCTAAAAAAGAGGAAAAAGAAAGAGCAAAATATATTGAAAAAC
AAAAATTAAAAGAAGAAAAATCGAATGGTTCAGTAAATAATAGTAATAATAATAGTAATA
GTAACAATAATAATAGTAATAATAATAATAATAATAGTAATAGTAGTAGTAATAATAATA
TAAGTGGAAATAAAGACAATAATAATTCAACACCAAATAAATCATTATCTTCCTCCTCAT
CTTCTGATAAAAAACAACAACCTTCATCACCTTCCTCTGCTGCCTCTCCTTCGACACCTT
CTTCACCTTCTCCCTTAGAAGGCACTTCTCCAAAACAAGATAAAAAAATTAACGCNCATA
TTAAATAGTAAAATT
Length of 3' end seq. 665
Connected seq. ID SFJ161P
Connected seq. 
>SFJ161P.Seq
ATTTTTAAGCATTATAATTAATACACATTTTGTTTTTTTTCCTCTTTTTTATTCTTTTAT
TTTCATTTTATTTTCCATTGTAAATATATTTAGTAAAAAATAAAATAAAATAAAATAGAA
TAATAAATAAAATAAAAAAGAATGTCATCTCCACCAACAACAACAACAACAACCACCACT
TCAACAGGCGGCAACCAAGAAACACCAAAAGCAATACCTCCAGCACCAATTGAAGATAAA
TCAGCTGAAAGAATAAAGAAAAGAGATCCAGGTAATATTACAAAGGTTATTAAATGTGGT
GTTGTAGGTGATGGTACTGTTGGAAAAACAACACTCTTATTATCTTATATCACTCAAGCA
TTCATTACAGAGTATACACCAACTGTATTTGATAACTTTTCAGCAATTGAAGAGGTTGAT
GATGGTAAGTTAGTAAATGTTATATTGTGGGATACTGCTGGTCAAGATGACTATGCACAA
ATCAGAACAACTTGTTATACAAATTGTAGATATGATGTATTTTTACTTTGTTTCTCTGTT
GTAAATCGTGATTCATTTGACAATGTTAAATATAAGT----------TATTTTAGTTGGT
ACAAAAACTGATATGAGAGATCAACAAACAGGAGGTTCACCAACCAATACTACATCATCA
CCACCAATTGCAGGTAATAATCAAACATTAAATAATATCATTACATTTAAAGAGGGTCAA
AAGAGAGCCAAAGAGATTAAAGCATTCAACTATATGGAATGTACATCTCGTGACTCTTCA
AGTGTTGCCAAGGTAGTTTTGGAGGCAATCAATATCATCATTGAAAAGGAAAAATTAAAA
AGAGTAAACAATGAAAAACTATGGAAGAGTCAAGTGAAAAAAGAAGAGAAAGAATCTAAA
AAAGAGGAAAAAGAAAGAGCAAAATATATTGAAAAACAAAAATTAAAAGAAGAAAAATCG
AATGGTTCAGTAAATAATAGTAATAATAATAGTAATAGTAACAATAATAATAGTAATAAT
AATAATAATAATAGTAATAGTAGTAGTAATAATAATATAAGTGGAAATAAAGACAATAAT
AATTCAACACCAAATAAATCATTATCTTCCTCCTCATCTTCTGATAAAAAACAACAACCT
TCATCACCTTCCTCTGCTGCCTCTCCTTCGACACCTTCTTCACCTTCTCCCTTAGAAGGC
ACTTCTCCAAAACAAGATAAAAAAATTAACGCNCATATTAAATAGTAAAATT
Length of connected seq. 1242
Full length Seq ID -
Full length Seq. -
Length of full length seq. -