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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFJ133
Library SF
(Link to library)
Clone ID SFJ133
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13863-1
Original site URL
Representative seq. ID SFJ133P
(Link to Original site)
Representative DNA sequence 
>SFJ133 (SFJ133Q) /CSM/SF/SFJ1-B/SFJ133Q.Seq.d/
AATAGTAATTATTTTTTTTTTCCTTCCCTCATTATTTTTTTTAAATAATAAATAAACATA
TNCAATGATTGATTCAGATNTAGAATTAAATAAAATAATAGTAGTACCATTAATATCAAA
AGATTCATTAGATATTAATGATGCAAAAAATTACTATGGAACCGAGTTNTACCCAAATCA
ACCAATTCCAACCATAGTTTCAGCTCAATCTGAAGTAGATTCTTTAATTAATCAAACTTN
TGGTAATAATGATGAANAAAGATTAGTTTTATTNTTAAAACANCANCXXXXXXXXXXCTC
CATTGGGATGTTTGTTAATGTCATTCAAATGTAAACAAACTGATGGTAATAAATTACCAT
TCCTTCAATATTTGGCTGGTATGGCAATGGTCGAAGCAATTCACTCTTTCCCAAGTGCAT
CTGATTTAAATCTTCGTTTGAAGTGGCCAAACGATATCTATAGTAATGAACCAGGTATGA
AAGTTGGTGGTGTCCTCTGTGAATCAAATTATCTTAACAATGAATTTGATGTTGTCATTG
GTGTTGGTGTTAATCTTTCAAATTCAAATAATCCATCAACAACTATAAATCAATTAATTC
AAAAGAAAAAATATGGATTTTCATCATCATCATTGGCAACAAGTACTGATATTCCAATTT
ATATTAGTAGAGAAGAATTGGTTTCAAAATTCTTTAACAAATTCGAACCAATGTTTATGG
AATTTACAAGAGATGGCTTTAATGCAGATCTTGAAAATAGATACACTGACTTATGGATGC
ATACAAATCAAATTGTAAAATTTAAAGAAAGAGATAACCATCATGTTAAAATCATTGGTA
TTTCTGATAGTGGTTTCCTTAAAGCTATTGAATGTGATGCTGATGGTAATACATCTTCTG
ACTCTGAAACCGTTGAGCTTCACCCAGATGGTACTTCATTNATATTCAAAACTTAATTTT
AAGCAAAAATCACAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iviiffflpslfflnnk*TYXMIDSDXELNKIIVVPLISKDSLDINDAKNYYGTEXYPNQ
PIPTIVSAQSEVDSLINQTXGNNDEXRLVLXLKXX---

---PLGCLLMSFKCKQTDGNKLPFLQYLAGMAMVEAIHSFPSASDLNLRLKWPNDIYSNE
PGMKVGGVLCESNYLNNEFDVVIGVGVNLSNSNNPSTTINQLIQKKKYGFSSSSLATSTD
IPIYISREELVSKFFNKFEPMFMEFTRDGFNADLENRYTDLWMHTNQIVKFKERDNHHVK
IIGISDSGFLKAIECDADGNTSSDSETVELHPDGTSXIFKT*f*akit
Translated Amino Acid sequence (All Frames) 
Frame A:
nsnyfffpsliiffk**inixnd*frxrik*nnsstinikrfiry**ckkllwnrvxpks
tnsnhsfssi*srffn*snxw****xkisfixktx---

---lhwdvc*chsnvnklmvinyhsfniwlvwqwskqftlsqvhli*ifv*sgqtisivm
nqv*klvvssvnqiiltmnlmlslvlvlifqiqiihqql*in*fkrknmdfhhhhwqqvl
ifqfilveknwfqnsltnsnqclwnlqemalmqilkidtltygciqikl*nlkkeitiml
kslvflivvslkllnvmlmvihlltlkplsftqmvlhxysklnfkqksq

Frame B:
iviiffflpslfflnnk*TYXMIDSDXELNKIIVVPLISKDSLDINDAKNYYGTEXYPNQ
PIPTIVSAQSEVDSLINQTXGNNDEXRLVLXLKXX---

---sigmfvnviqm*tn*w**itipsifgwygngrsnslfpkci*fkssfevakryl***
tryeswwcpl*ikls*q*i*cchwcwc*sfkfk*sinnyksinskekiwifiiiignky*
ysnly**rrigfkil*qirtnvygiykrwl*crs*k*ih*lmdayksncki*rkr*psc*
nhwyf**wfp*sy*m*c*w*yif*l*nr*asprwyfixiqnlilsknh

Frame C:
**lfffsfphyff*iinkhxq*liqx*n*ik***yh*yqkih*ilmmqkitmepsxtqin
qfqp*fqlnlk*il*liklxvimmxkd*fyx*nxx---

---PLGCLLMSFKCKQTDGNKLPFLQYLAGMAMVEAIHSFPSASDLNLRLKWPNDIYSNE
PGMKVGGVLCESNYLNNEFDVVIGVGVNLSNSNNPSTTINQLIQKKKYGFSSSSLATSTD
IPIYISREELVSKFFNKFEPMFMEFTRDGFNADLENRYTDLWMHTNQIVKFKERDNHHVK
IIGISDSGFLKAIECDADGNTSSDSETVELHPDGTSXIFKT*f*akit
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFJ133 (SFJ133Q) /CSM/SF/SFJ1-B/SFJ133Q.Seq.d/                       1737   0.0
SFD294 (SFD294Q) /CSM/SF/SFD2-D/SFD294Q.Seq.d/                       1338   0.0
SFF405 (SFF405Q) /CSM/SF/SFF4-A/SFF405Q.Seq.d/                       1279   0.0
CHB590 (CHB590Q) /CSM/CH/CHB5-D/CHB590Q.Seq.d/                       1279   0.0
AFC713 (AFC713Q) /CSM/AF/AFC7-A/AFC713Q.Seq.d/                       1174   0.0
SFE209 (SFE209Q) /CSM/SF/SFE2-A/SFE209Q.Seq.d/                       1102   0.0
AFN413 (AFN413Q) /CSM/AF/AFN4-A/AFN413Q.Seq.d/                        918   0.0
AFO680 (AFO680Q) /CSM/AF/AFO6-D/AFO680Q.Seq.d/                        914   0.0
AFH686 (AFH686Q) /CSM/AF/AFH6-D/AFH686Q.Seq.d/                        842   0.0
SFI426 (SFI426Q) /CSM/SF/SFI4-B/SFI426Q.Seq.d/                        827   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   9e-09  24
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    34   5e-08  16
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   3e-07  20
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36   7e-07  18
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    36   9e-07  18
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    34   2e-06  15
AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    42   4e-06  13
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   6e-06  21
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    38   3e-05  14
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           34   3e-05  25
dna update 2004. 1. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP008208_425(AP008208|pid:none) Oryza sativa (japonica cultivar-...   137   7e-31
AP005804_18(AP005804|pid:none) Oryza sativa Japonica Group genom...   134   3e-30
AC006053_17(AC006053|pid:none) Arabidopsis thaliana chromosome 2...   131   3e-29
G84651(G84651)biotin holocarboxylase synthetase [imported] - Ara...   129   1e-28
AF414940_1(AF414940|pid:none) Arabidopsis thaliana holocarboxyla...   129   1e-28
CP000582_324(CP000582|pid:none) Ostreococcus lucimarinus CCE9901...   124   4e-27
(P50747) RecName: Full=Biotin--protein ligase;          EC=6.3.4...   122   2e-26
AK168925_1(AK168925|pid:none) Mus musculus 16 days embryo kidney...   117   8e-25
(Q920N2) RecName: Full=Biotin--protein ligase;          EC=6.3.4...   117   8e-25
BC153857_1(BC153857|pid:none) Bos taurus similar to holocarboxyl...   114   5e-24
protein update 2009. 5.26
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SFJ133 is cyt
5' end seq. ID SFJ133F
5' end seq. 
>SFJ133F.Seq
AATAGTAATTATTTTTTTTTTCCTTCCCTCATTATTTTTTTTAAATAATAAATAAACATA
TNCAATGATTGATTCAGATNTAGAATTAAATAAAATAATAGTAGTACCATTAATATCAAA
AGATTCATTAGATATTAATGATGCAAAAAATTACTATGGAACCGAGTTNTACCCAAATCA
ACCAATTCCAACCATAGTTTCAGCTCAATCTGAAGTAGATTCTTTAATTAATCAAACTTN
TGGTAATAATGATGAANAAAGATTAGTTTTATTNTTAAAACANCANC----------
Length of 5' end seq. 287
3' end seq. ID SFJ133Z
3' end seq. 
>SFJ133Z.Seq
----------CTCCATTGGGATGTTTGTTAATGTCATTCAAATGTAAACAAACTGATGGT
AATAAATTACCATTCCTTCAATATTTGGCTGGTATGGCAATGGTCGAAGCAATTCACTCT
TTCCCAAGTGCATCTGATTTAAATCTTCGTTTGAAGTGGCCAAACGATATCTATAGTAAT
GAACCAGGTATGAAAGTTGGTGGTGTCCTCTGTGAATCAAATTATCTTAACAATGAATTT
GATGTTGTCATTGGTGTTGGTGTTAATCTTTCAAATTCAAATAATCCATCAACAACTATA
AATCAATTAATTCAAAAGAAAAAATATGGATTTTCATCATCATCATTGGCAACAAGTACT
GATATTCCAATTTATATTAGTAGAGAAGAATTGGTTTCAAAATTCTTTAACAAATTCGAA
CCAATGTTTATGGAATTTACAAGAGATGGCTTTAATGCAGATCTTGAAAATAGATACACT
GACTTATGGATGCATACAAATCAAATTGTAAAATTTAAAGAAAGAGATAACCATCATGTT
AAAATCATTGGTATTTCTGATAGTGGTTTCCTTAAAGCTATTGAATGTGATGCTGATGGT
AATACATCTTCTGACTCTGAAACCGTTGAGCTTCACCCAGATGGTACTTCATTNATATTC
AAAACTTAATTTTAAGCAAAAATCACAA
Length of 3' end seq. 678
Connected seq. ID SFJ133P
Connected seq. 
>SFJ133P.Seq
AATAGTAATTATTTTTTTTTTCCTTCCCTCATTATTTTTTTTAAATAATAAATAAACATA
TNCAATGATTGATTCAGATNTAGAATTAAATAAAATAATAGTAGTACCATTAATATCAAA
AGATTCATTAGATATTAATGATGCAAAAAATTACTATGGAACCGAGTTNTACCCAAATCA
ACCAATTCCAACCATAGTTTCAGCTCAATCTGAAGTAGATTCTTTAATTAATCAAACTTN
TGGTAATAATGATGAANAAAGATTAGTTTTATTNTTAAAACANCANC----------CTC
CATTGGGATGTTTGTTAATGTCATTCAAATGTAAACAAACTGATGGTAATAAATTACCAT
TCCTTCAATATTTGGCTGGTATGGCAATGGTCGAAGCAATTCACTCTTTCCCAAGTGCAT
CTGATTTAAATCTTCGTTTGAAGTGGCCAAACGATATCTATAGTAATGAACCAGGTATGA
AAGTTGGTGGTGTCCTCTGTGAATCAAATTATCTTAACAATGAATTTGATGTTGTCATTG
GTGTTGGTGTTAATCTTTCAAATTCAAATAATCCATCAACAACTATAAATCAATTAATTC
AAAAGAAAAAATATGGATTTTCATCATCATCATTGGCAACAAGTACTGATATTCCAATTT
ATATTAGTAGAGAAGAATTGGTTTCAAAATTCTTTAACAAATTCGAACCAATGTTTATGG
AATTTACAAGAGATGGCTTTAATGCAGATCTTGAAAATAGATACACTGACTTATGGATGC
ATACAAATCAAATTGTAAAATTTAAAGAAAGAGATAACCATCATGTTAAAATCATTGGTA
TTTCTGATAGTGGTTTCCTTAAAGCTATTGAATGTGATGCTGATGGTAATACATCTTCTG
ACTCTGAAACCGTTGAGCTTCACCCAGATGGTACTTCATTNATATTCAAAACTTAATTTT
AAGCAAAAATCACAA
Length of connected seq. 965
Full length Seq ID -
Full length Seq. -
Length of full length seq. -