>SFI846 (SFI846Q) /CSM/SF/SFI8-B/SFI846Q.Seq.d/
XXXXXXXXXXTTATCAATTCAACATGGTTTTAAATTACCAATAATTGAAAAGGTATTAGA
TCTTGTATTTTTCTCAAAAATTAAACAAGCATTAGGTGGAAAAGTTAGAGTCATTCTATC
AGGTTCTGCTCCATTAAGTTTTGATACTGAAGTATTTTTAAGAGTTGTTATGTGTTGTTG
TGTTTTACAAAGTTATGGTGCAAGTGAAGGCTGTGGTGGTGATGCATGTAAAAGATTAGA
TGATGAATCAGTTGGTACAATTGGACCACCATTTGCAAGTAATGAAATTAAATTAGTTGA
TGTACCAGAGTTGGGTTATGATAGTAATGGTGAAGTACAAACTGGTGAAGTATGTCTTCG
TGGTCCAAGTATCTCAAGTGGTTATTATAAAGATGAGGAGAAAACTAGAGAAGAATTCAA
AGATGGTTGGTTCCATACTGGTGATATTGGTCGTTGGAATCGTGATGGTAGTCTTAGCAT
TGTTGATCGTAAGAAAAATATCTTTAAATTATCTCAAGGTGAATATGTTGCAGTTGAAAA
GATTGAAACAATAGTTGTAAAGAGTGAATACGTTGAACAAGTTTGTATTTATGG

---SIQHGFKLPIIEKVLDLVFFSKIKQALGGKVRVILSGSAPLSFDTEVFLRVVMCCCV
LQSYGASEGCGGDACKRLDDESVGTIGPPFASNEIKLVDVPELGYDSNGEVQTGEVCLRG
PSISSGYYKDEEKTREEFKDGWFHTGDIGRWNRDGSLSIVDRKKNIFKLSQGEYVAVEKI
ETIVVKSEYVEQVCIY

Frame A:
---yqfnmvlnyq*lkry*ilyfsqklnkh*veklesfyqvllh*vlilkyf*ellcvvv
fykvmvqvkavvvmhvkd*mmnqlvqldhhlqvmkln*lmyqswvmivmvkyklvkyvfv
vqvsqvviikmrrkleknskmvgsilvilvvgivmvvlallivrkislnylkvnmlqlkr
lkq*l*rvntlnkfvfm


Frame B:
---instwf*itnn*kgirsciflkn*tsirwks*shsirfcsikf*y*sifkscyvllc
ftklwck*rlww*cm*kir**iswynwttick**n*is*ctrvgl***w*stnw*smssw
skylkwll*r*gen*rriqrwlvpyw*ywsles*w*s*hc*s*ekyl*iisr*iccs*kd
*nnscke*ir*tslylw


Frame C:
---SIQHGFKLPIIEKVLDLVFFSKIKQALGGKVRVILSGSAPLSFDTEVFLRVVMCCCV
LQSYGASEGCGGDACKRLDDESVGTIGPPFASNEIKLVDVPELGYDSNGEVQTGEVCLRG
PSISSGYYKDEEKTREEFKDGWFHTGDIGRWNRDGSLSIVDRKKNIFKLSQGEYVAVEKI
ETIVVKSEYVEQVCIY

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI846 (SFI846Q) /CSM/SF/SFI8-B/SFI846Q.Seq.d/                       1158   0.0
SLE702 (SLE702Q) /CSM/SL/SLE7-A/SLE702Q.Seq.d/                        878   0.0
AFE482 (AFE482Q) /CSM/AF/AFE4-D/AFE482Q.Seq.d/                        823   0.0
CHN511 (CHN511Q) /CSM/CH/CHN5-A/CHN511Q.Seq.d/                        799   0.0
CHG452 (CHG452Q) /CSM/CH/CHG4-C/CHG452Q.Seq.d/                        799   0.0
AHB382 (AHB382Q) /CSM/AH/AHB3-D/AHB382Q.Seq.d/                        799   0.0
AFG590 (AFG590Q) /CSM/AF/AFG5-D/AFG590Q.Seq.d/                        799   0.0
AHJ607 (AHJ607Q) /CSM/AH/AHJ6-A/AHJ607Q.Seq.d/                        797   0.0
AFO720 (AFO720Q) /CSM/AF/AFO7-A/AFO720Q.Seq.d/                        781   0.0
CHR758 (CHR758Q) /CSM/CH/CHR7-C/CHR758Q.Seq.d/                        753   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY196478|AY196478.1 Dictyostelium discoideum fatty acyl-CoA synthetase (fcsA) mRNA, complete cds.    82   2e-30  2
BQ875501|BQ875501.1 QGI8E20.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGI8E20, mRNA sequence.    62   2e-12  2
BQ870927|BQ870927.1 QGI10G21.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGI10G21, mRNA sequence.    62   3e-12  2
BU014148|BU014148.1 QGJ6J03.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGJ6J03, mRNA sequence.    48   2e-08  2
BU723159|BU723159.1 SJMARH09 SJM Schistosoma japonicum cDNA, mRNA sequence.    50   6e-06  2
AL022304|AL022304.1 S.pombe chromosome II cosmid c18H10.           44   8e-06  2
AU010454|AU010454.1 Schizosaccharomyces pombe cDNA, clone spc05909.    44   1e-05  2
AU010407|AU010407.1 Schizosaccharomyces pombe cDNA, clone spc05821.    44   1e-05  2
CF923027|CF923027.1 gmrhRww24-02-SP6_G05_1_035 Soybean root hair subtracted cDNA library gmrhRww24 Glycine max cDNA, mRNA sequence.    60   4e-05  1
BG511003|BG511003.1 sac81c11.y1 Gm-c1072 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1072-1677 5' similar to TR:Q9XIA9 Q9XIA9 PUTATIVE ACYL COA SYNTHETASE. ;, mRNA sequence.    44   4e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY196478_1(AY196478|pid:none) Dictyostelium discoideum fatty acy...   204   1e-51
AL049876_1(AL049876|pid:none) Arabidopsis thaliana DNA chromosom...   204   2e-51
AF080118_2(AF080118|pid:none) Arabidopsis thaliana BAC F8M12.  &...   204   2e-51
DQ174259_1(DQ174259|pid:none) Gossypium hirsutum ACS-like protei...   203   3e-51
T07929(T07929) probable long-chain-fatty-acid-CoA ligase (EC 6.2...   196   3e-49
EU616571_1(EU616571|pid:none) Capsicum annuum clone a putative l...   196   4e-49
EU956770_1(EU956770|pid:none) Zea mays clone 1573252 ACS-like pr...   196   5e-49
DQ174260_1(DQ174260|pid:none) Gossypium hirsutum ACS-like protei...   194   2e-48
DQ900687_3(DQ900687|pid:none) Hordeum vulgare clone BAC 455J22, ...   194   2e-48
AP004363_17(AP004363|pid:none) Oryza sativa Japonica Group genom...   193   3e-48

 psg: 0.73  gvh: 0.56  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SFI846 is cyt

>SFI846Z.Seq
NNNNNNNNNNTTATCAATTCAACATGGTTTTAAATTACCAATAATTGAAAAGGTATTAGA
TCTTGTATTTTTCTCAAAAATTAAACAAGCATTAGGTGGAAAAGTTAGAGTCATTCTATC
AGGTTCTGCTCCATTAAGTTTTGATACTGAAGTATTTTTAAGAGTTGTTATGTGTTGTTG
TGTTTTACAAAGTTATGGTGCAAGTGAAGGCTGTGGTGGTGATGCATGTAAAAGATTAGA
TGATGAATCAGTTGGTACAATTGGACCACCATTTGCAAGTAATGAAATTAAATTAGTTGA
TGTACCAGAGTTGGGTTATGATAGTAATGGTGAAGTACAAACTGGTGAAGTATGTCTTCG
TGGTCCAAGTATCTCAAGTGGTTATTATAAAGATGAGGAGAAAACTAGAGAAGAATTCAA
AGATGGTTGGTTCCATACTGGTGATATTGGTCGTTGGAATCGTGATGGTAGTCTTAGCAT
TGTTGATCGTAAGAAAAATATCTTTAAATTATCTCAAGGTGAATATGTTGCAGTTGAAAA
GATTGAAACAATAGTTGTAAAGAGTGAATACGTTGAACAAGTTTGTATTTATGG