>AFI453 (AFI453Q) /CSM/AF/AFI4-C/AFI453Q.Seq.d/
XXXXXXXXXXCCCATTCGCTGNAACCACCGATGGTCAAAGTCATTTGTATGGATGCTAAA
ATCAATTTCGATGACAATGCCTCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACC
GCCGAAGAGGATCCACGTGAGGTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTC
GATGGTAACATTGGTTGTATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATC
ATTAAATTAAAGGGTGGTATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAA
CAAGCCGTCACTGAAGCCTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTC
GTAAACATTTTCGGTGGTATCATGAAATGTGATATCATTGCCTCTGGTATCGTAAATGCT
TCAAAACAAGTTGGTCTTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAAC
ATTGGTAAAGAAATCTTGGAAAAATGCTGGTTTAAATATTACTTCTGCTAGTGAATTTAG
ATGATGCTGCTATTANAGGCTGTTAATTGTTTAAA

---HSLXPPMVKVICMDAKINFDDNASFRQKEIFEMRDTAEEDPREVEAGKFGLNYIGLD
GNIGCMVNGAGLAMATMDIIKLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKCLLV
NIFGGIMKCDIIASGIVNASKQVGLKIPLVVRLEGTNVNIGKEILEKCWFKYYFC**i*m
mlllxavncl

Frame A:
---pfaxttdgqshlygc*nqfr*qclipskgdfrneryrrrgst*g*sw*iwsqlhwsr
w*hwlyg*wcwfgyghhgyh*ikgwypc*fprcrw*cl*tsrh*slqnph*rsks*mftr
khfrwyhem*yhclwyrkcfktsws*nsisctfrrykckhw*rnlgkmlv*illllvnld
daaixgc*lfk


Frame B:
---HSLXPPMVKVICMDAKINFDDNASFRQKEIFEMRDTAEEDPREVEAGKFGLNYIGLD
GNIGCMVNGAGLAMATMDIIKLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKCLLV
NIFGGIMKCDIIASGIVNASKQVGLKIPLVVRLEGTNVNIGKEILEKCWFKYYFC**i*m
mlllxavncl


Frame C:
---irxnhrwsksfvwmlksismtmphsvkrrfsk*eippkrihvrlklvnlvsitlvsm
vtlvvwlmvlvwlwppwisln*rvvsllissm*vvvplnkpslkpsksslkiqelnvys*
tfsvvs*nvislplvs*mlqnklvlkfh*lyv*kvqm*tlvkkswknaglnitsasefr*
ccyxrlliv*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI453 (AFI453Q) /CSM/AF/AFI4-C/AFI453Q.Seq.d/                       1108   0.0
SSI167 (SSI167Q) /CSM/SS/SSI1-C/SSI167Q.Seq.d/                       1045   0.0
VFH579 (VFH579Q) /CSM/VF/VFH5-D/VFH579Q.Seq.d/                       1039   0.0
VFG281 (VFG281Q) /CSM/VF/VFG2-D/VFG281Q.Seq.d/                       1029   0.0
VFN464 (VFN464Q) /CSM/VF/VFN4-C/VFN464Q.Seq.d/                       1023   0.0
VFM201 (VFM201Q) /CSM/VF/VFM2-A/VFM201Q.Seq.d/                       1023   0.0
VFL479 (VFL479Q) /CSM/VF/VFL4-D/VFL479Q.Seq.d/                       1023   0.0
VFC521 (VFC521Q) /CSM/VF/VFC5-A/VFC521Q.Seq.d/                       1023   0.0
SLG726 (SLG726Q) /CSM/SL/SLG7-B/SLG726Q.Seq.d/                       1023   0.0
SFJ195 (SFJ195Q) /CSM/SF/SFJ1-D/SFJ195Q.Seq.d/                       1023   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   928   0.0    5
X84222|X84222.2 Neocallimastix frontalis mRNA for beta-succinyl CoA synthetase  precursor.    74   1e-21  4
AF493055|AF493055.1 Neocallimastix patriciarum succinyl-CoA synthetase, beta subunit (scsB) mRNA, complete cds.    74   2e-21  4
AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.    62   3e-21  5
AX621832|AX621832.1 Sequence 4795 from Patent WO02094868.          52   1e-19  4
AR354123|AR354123.1 Sequence 241 from patent US 6593114.           52   1e-17  4
BJ519880|BJ519880.1 Oryzias latipes cDNA clone:MF01SSB014A03, 5' end single read.    70   2e-16  2
BJ532800|BJ532800.1 Oryzias latipes cDNA clone:MF01SSB015A03, 3' end single read.    70   2e-16  2
BJ520215|BJ520215.1 Oryzias latipes cDNA clone:MF01SSB015A03, 5' end single read.    70   2e-16  2
AP003133|AP003133.2 Staphylococcus aureus subsp. aureus N315 genomic DNA, complete genome, section 5/10.    52   4e-16  7

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q6K9N6) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   246   2e-64
BT039713_1(BT039713|pid:none) Zea mays full-length cDNA clone ZM...   245   5e-64
AK221149_1(AK221149|pid:none) Arabidopsis thaliana mRNA for succ...   241   1e-62
(O82662) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   241   1e-62
DQ200398_1(DQ200398|pid:none) Solanum tuberosum clone 071C05 suc...   239   4e-62
DQ294279_1(DQ294279|pid:none) Solanum tuberosum clone 180E05 suc...   239   4e-62
AY087596_1(AY087596|pid:none) Arabidopsis thaliana clone 36904 m...   238   6e-62
EF530042_1(EF530042|pid:none) Caragana jubata succinyl-CoA ligas...   237   2e-61
(A4IM83) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   231   8e-60
CP000922_1730(CP000922|pid:none) Anoxybacillus flavithermus WK1,...   231   8e-60

 psg: 0.73  gvh: 0.23  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for AFI453 is cyt

>AFI453Z.Seq
----------CCCATTCGCTGNAACCACCGATGGTCAAAGTCATTTGTATGGATGCTAAA
ATCAATTTCGATGACAATGCCTCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACC
GCCGAAGAGGATCCACGTGAGGTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTC
GATGGTAACATTGGTTGTATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATC
ATTAAATTAAAGGGTGGTATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAA
CAAGCCGTCACTGAAGCCTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTC
GTAAACATTTTCGGTGGTATCATGAAATGTGATATCATTGCCTCTGGTATCGTAAATGCT
TCAAAACAAGTTGGTCTTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAAC
ATTGGTAAAGAAATCTTGGAAAAATGCTGGTTTAAATATTACTTCTGCTAGTGAATTTAG
ATGATGCTGCTATTANAGGCTGTTAATTGTTTAAA