>AFI447 (AFI447Q) /CSM/AF/AFI4-B/AFI447Q.Seq.d/
XXXXXXXXXXCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAG
CTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCC
AAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAA
ATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCAC
CAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTC
CAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGA
TTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCA
TGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATC
ATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCAT
CTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCTAAACA

---SGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESN
DAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKI
DADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSS
QTDDNVNLKKILLLN

Frame A:
---SGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESN
DAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKI
DADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSS
QTDDNVNLKKILLLN


Frame B:
---lvklfqpslliqlpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqm
mlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rl
mptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhl
klmimxi*krffc*t


Frame C:
---w*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*
css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*
crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rch*iqitiis
n***cxskkdssak

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL328 (VFL328Q) /CSM/VF/VFL3-B/VFL328Q.Seq.d/                       1140   0.0
VFK816 (VFK816Q) /CSM/VF/VFK8-A/VFK816Q.Seq.d/                       1140   0.0
VFK745 (VFK745Q) /CSM/VF/VFK7-B/VFK745Q.Seq.d/                       1140   0.0
SFL246 (SFL246Q) /CSM/SF/SFL2-B/SFL246Q.Seq.d/                       1140   0.0
SFK855 (SFK855Q) /CSM/SF/SFK8-C/SFK855Q.Seq.d/                       1140   0.0
SFI119 (SFI119Q) /CSM/SF/SFI1-A/SFI119Q.Seq.d/                       1140   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1140   0.0
AFO474 (AFO474Q) /CSM/AF/AFO4-D/AFO474Q.Seq.d/                       1140   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1140   0.0
AFO351 (AFO351Q) /CSM/AF/AFO3-C/AFO351Q.Seq.d/                       1140   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   4e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   4e-08  1
CK029548|CK029548.1 AGENCOURT_16620510 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7051751 5', mRNA sequence.    70   4e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   4e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   168   1e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   162   8e-39
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   157   3e-37
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   155   7e-37
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   155   7e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   1e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   1e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   148   9e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   148   9e-35
BC097044_1(BC097044|pid:none) Danio rerio protein disulfide isom...   147   2e-34

 psg: 0.55  gvh: 0.38  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFI447 is nuc

>AFI447Z.Seq
----------CTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAG
CTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCC
AAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAA
ATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCAC
CAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTC
CAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGA
TTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCA
TGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATC
ATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCAT
CTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCTAAACA