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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI444
Library AF
(Link to library)
Clone ID AFI444
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID AFI444Z
(Link to Original site)
Representative DNA sequence 
>AFI444 (AFI444Q) /CSM/AF/AFI4-B/AFI444Q.Seq.d/
XXXXXXXXXXAAACTATCACTTTGNAAGTTGAAGGTTTTNATAATATTNAAAATGTAAAG
GCTAAAATCCAAGATAAAAAAGGTATTNCACCNGATCAACAAANNTTNATTTTTGCCGGT
AANCAATTAGNAGATGGTCGNCTCTTTNTGACTACAATATTCAAAAAGAATCCNCTCTCC
ATTTAGTTTTAAGATTAAGAGGTGGTATGCAAATCTTTGTTAAAACTCNNACTGGNNAAN
CAATCACTTTANAAGTTGAAGGTTCTGANAACATTGGNAATGTTAAAGCCAAGATCCAAG
ATAAAGAAGGTATTCCNCCAGATCAACAAAGATTAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
---nyhfxs*rfx*yxkckg*npr*kryxtxstxxxfcr*XIXRWSXLXDYNIQKESXLH
LVLRLRGGMQIFVKTXTGXXITLXVEGSXNIGNVKAKIQDKEGIPPDQQRL
Translated Amino Acid sequence (All Frames) 
Frame A:
---nyhfxs*rfx*yxkckg*npr*kryxtxstxxxfcr*XIXRWSXLXDYNIQKESXLH
LVLRLRGGMQIFVKTXTGXXITLXVEGSXNIGNVKAKIQDKEGIPPDQQRL


Frame B:
---titlxvegfxnixnvkakiqdkkgixpdqqxxifagxqlxdgrlfxttifkknplsi
*f*d*evvcksllklxlxxqslxklkvlxtlxmlkprskikkvfxqinkd*


Frame C:
---lslxklkvxiixkm*rlkskikkvxhxinkxxflpvxn*xmvxsx*lqyskrixspf
sfkikrwyanlc*nsxwxxnhfxs*rf*xhwxc*sqdpr*rrysxrstki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI444 (AFI444Q) /CSM/AF/AFI4-B/AFI444Q.Seq.d/                        515   e-145
VFK831 (VFK831Q) /CSM/VF/VFK8-B/VFK831Q.Seq.d/                        472   e-132
SFJ463 (SFJ463Q) /CSM/SF/SFJ4-C/SFJ463Q.Seq.d/                        472   e-132
SFJ247 (SFJ247Q) /CSM/SF/SFJ2-B/SFJ247Q.Seq.d/                        472   e-132
SFI665 (SFI665Q) /CSM/SF/SFI6-C/SFI665Q.Seq.d/                        472   e-132
AFK824 (AFK824Q) /CSM/AF/AFK8-A/AFK824Q.Seq.d/                        472   e-132
VFI474 (VFI474Q) /CSM/VF/VFI4-D/VFI474Q.Seq.d/                        470   e-132
SSM248 (SSM248Q) /CSM/SS/SSM2-B/SSM248Q.Seq.d/                        470   e-132
SSI334 (SSI334Q) /CSM/SS/SSI3-B/SSI334Q.Seq.d/                        470   e-132
SSG823 (SSG823Q) /CSM/SS/SSG8-A/SSG823Q.Seq.d/                        470   e-132
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   313   e-128  2
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   274   2e-94  2
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   210   1e-88  2
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   210   1e-88  2
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   210   3e-73  3
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.   111   6e-62  3
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.   111   9e-62  3
M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.   111   9e-62  3
CF181874|CF181874.1 ISO1H6R Iso cDNA Isotricha sp. BBF-2003 cDNA similar to PfpUB polyubiquitin 3D7, mRNA sequence.   178   3e-41  1
CF181989|CF181989.1 ISO4G3F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.   178   3e-41  1
dna update 2004. 6.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   109   2e-36
B27806(B27806) ubiquitin (clone lambda229) - slime mold (Dictyos...   109   2e-36
A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   109   6e-36
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   109   6e-36
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   109   6e-36
AM117576_1(AM117576|pid:none) Alvinella caudata partial gene for...   104   3e-34
DQ363465_1(DQ363465|pid:none) Ictalurus punctatus clone C153BB02...   107   4e-34
Z35759_1(Z35759|pid:none) T.pyriformis (CGL) polyubiquitin gene ...   104   7e-34
X98063_1(X98063|pid:none) P.sylvestris mRNA for polyubiquitin.        105   9e-34
AC149642_19(AC149642|pid:none) Medicago truncatula chromosome 2 ...   105   9e-34
protein update 2009. 4.23
PSORT 

 psg: 0.52  gvh: 0.49  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for AFI444 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFI444Z
3' end seq. 
>AFI444Z.Seq
----------AAACTATCACTTTGNAAGTTGAAGGTTTTNATAATATTNAAAATGTAAAG
GCTAAAATCCAAGATAAAAAAGGTATTNCACCNGATCAACAAANNTTNATTTTTGCCGGT
AANCAATTAGNAGATGGTCGNCTCTTTNTGACTACAATATTCAAAAAGAATCCNCTCTCC
ATTTAGTTTTAAGATTAAGAGGTGGTATGCAAATCTTTGTTAAAACTCNNACTGGNNAAN
CAATCACTTTANAAGTTGAAGGTTCTGANAACATTGGNAATGTTAAAGCCAAGATCCAAG
ATAAAGAAGGTATTCCNCCAGATCAACAAAGATTAA
Length of 3' end seq. 326
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -