>SFI255 (SFI255Q) /CSM/SF/SFI2-C/SFI255Q.Seq.d/
AAAGTGATGAAGATGTAAAAACCACTACAACCACTACTACTTCAACATCAACAAAACCAC
TCACTGAAATTTTTCGATGGAATTGTATTTTGTTTTTCAGGTACATTTTCAGTAACACAA
ACTGAATTAACAAAAAAAGTTGAAGGTTGTGGCGCATCAACCTCAAXXXXXXXXXXGAAA
CCAACCATTGTAAACATTTTCAAGATTGATCGTGATGGTGAAGCAGATCGTTATAAAACT
AAAAAACATNTTGGTAATAGAAAACTCTTATGGCATGGTTCTCGTTTAACAAATTATGCT
TCCATCATTTCACAAGGTTTAANAATTGCTCCACCTGAAGCTCCAGTTTCTGGTTATANA
TTTGGAAAAGGTGTTTATTTTGCTGATTGTATGTCACTTTCTGCAAATTATTGTAGAACA
GTTGGATCTAATGATTTTTGTATGTTATTAGGTGATGTTGCATTGGGTAAAACTGCAGAT
TTGGCTANAGATACTTATATGGAGAAACCACAACCAAATTNTCATTCAACTTGGGCTTTA
GGTACTGTTGAACCAGATCCAAAAGTATTTTTCCAAGATACTGAAGGTTGTATCACTCCA
TACGGCCAAATGATTCCATCTCAACATAAAGGTGTTTCNTGTTATGAACATCAATATGTC
GTTTATGATGTTGCTCAAGTTCATTTGAAATATCTTTTGCAATTAAGAAATTAATAAACT
ATATTTAATTGTATAATAATAATAATAATAATAAAATAAAAAAAATAAAAAAATAAAATA
AAAANAATAATAAAA

kvmkm*KPLQPLLLQHQQNHSLKFFDGIVFCFSGTFSVTQTELTKKVEGCGASTS---

---KPTIVNIFKIDRDGEADRYKTKKHXGNRKLLWHGSRLTNYASIISQGLXIAPPEAPV
SGYXFGKGVYFADCMSLSANYCRTVGSNDFCMLLGDVALGKTADLAXDTYMEKPQPNXHS
TWALGTVEPDPKVFFQDTEGCITPYGQMIPSQHKGVSCYEHQYVVYDVAQVHLKYLLQLR
N**tifnciiiiiiik*kk*knkikxiik

Frame A:
kvmkm*KPLQPLLLQHQQNHSLKFFDGIVFCFSGTFSVTQTELTKKVEGCGASTS---

---etnhckhfqd*s*w*srsl*n*ktxw**ktlmawfsfnklcfhhftrfxncst*sss
fwlxiwkrclfc*lyvtfckll*nswi**flyvir*ccig*ncrfgxrylygetttkxsf
nlgfryc*trsksifpry*rlyhsirpndsist*rcfxl*tsicrl*ccsssfeisfaik
klinyi*lynnnnnnkikkikk*nkxnnk

Frame B:
k**rcknhynhyyfninktth*nfsmelyfvfqvhfq*hkln*qkklkvvahqpq---

---KPTIVNIFKIDRDGEADRYKTKKHXGNRKLLWHGSRLTNYASIISQGLXIAPPEAPV
SGYXFGKGVYFADCMSLSANYCRTVGSNDFCMLLGDVALGKTADLAXDTYMEKPQPNXHS
TWALGTVEPDPKVFFQDTEGCITPYGQMIPSQHKGVSCYEHQYVVYDVAQVHLKYLLQLR
N**tifnciiiiiiik*kk*knkikxiik

Frame C:
sdedvktttttttststkplteifrwncilffryifsntn*inkks*rlwrinl---

---nqpl*tfsrlivmvkqiviklknxlviensygmvlv*qimlpsfhkv*xllhlklqf
lvixlekvfillivchflqiiveqldlmifvcy*vmlhwvklqiwlxiliwrnhnqixiq
lgl*vllnqiqkyfskilkvvslhtak*fhlnikvfxvmninmsfmmllkfi*nifcn*e
inklyliv*******nkknkkik*kx**

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI255 (SFI255Q) /CSM/SF/SFI2-C/SFI255Q.Seq.d/                       1314   0.0
VHG617 (VHG617Q) /CSM/VH/VHG6-A/VHG617Q.Seq.d/                       1066   0.0
VHH451 (VHH451Q) /CSM/VH/VHH4-C/VHH451Q.Seq.d/                       1031   0.0
CHS286 (CHS286Q) /CSM/CH/CHS2-D/CHS286Q.Seq.d/                       1013   0.0
CHO733 (CHO733Q) /CSM/CH/CHO7-B/CHO733Q.Seq.d/                       1005   0.0
VHB207 (VHB207Q) /CSM/VH/VHB2-A/VHB207Q.Seq.d/                        987   0.0
CHO833 (CHO833Q) /CSM/CH/CHO8-B/CHO833Q.Seq.d/                        880   0.0
SLD235 (SLD235Q) /CSM/SL/SLD2-B/SLD235Q.Seq.d/                        359   4e-98
VHM584 (VHM584Q) /CSM/VH/VHM5-D/VHM584Q.Seq.d/                         56   8e-07
VHL657 (VHL657Q) /CSM/VH/VHL6-C/VHL657Q.Seq.d/                         56   8e-07

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ520099|AJ520099.1 Dictyostelium discoideum mRNA for (ADP-ribosyl)transferase (adprt2  gene).  1067   0.0    3
CD169660|CD169660.1 MM1-0024T-M080-E06-U.G MM1-0024 Schistosoma mansoni cDNA clone MM1-0024T-M080-E06.G, mRNA sequence.    84   3e-12  1
CD169680|CD169680.1 MM1-0024T-M080-G03-U.G MM1-0024 Schistosoma mansoni cDNA clone MM1-0024T-M080-G03.G, mRNA sequence.    84   3e-12  1
CD068585|CD068585.1 MA2-0002P-V059-B02-U.B MA2-0002 Schistosoma mansoni cDNA clone MA2-0002P-V059-B02.B, mRNA sequence.    84   3e-12  1
AJ534948|AJ534948.1 Dictyostelium discoideum mRNA for NAD(+) ADP-ribosyltransferase-1A (adprt-1A gene).    56   3e-11  3
CF445056|CF445056.1 EST681401 normalized cDNA library of onion Allium cepa cDNA clone ACAIF62, mRNA sequence.    46   2e-09  3
CF439652|CF439652.1 EST675997 normalized cDNA library of onion Allium cepa cDNA clone ACAAS01, mRNA sequence.    46   2e-09  3
BU715787|PPOL_ARATH POLY [ADP-RIBOSE] POLYMERASE (PARP) (ADPRT) (NAD(+) ADP-RIBOSYLTRANSFERASE) (POLY[ADP-RIBOSE] SYNTHETASE), mRNA sequence.    56   4e-09  2
BJ222440|BJ222440.1 Triticum aestivum cDNA clone:whdl13g14, 5' end, single read.    44   5e-05  2
CD129952|CD129952.1 MF1-0031U-L048-F06-U.B MF1-0031 Schistosoma mansoni cDNA clone MF1-0031U-L048-F06.B, mRNA sequence.    60   5e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ520099_1(AJ520099|pid:none) Dictyostelium discoideum mRNA for ...   364   1e-99
(Q9R152) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   172   1e-41
AK159433_1(AK159433|pid:none) Mus musculus osteoclast-like cell ...   171   2e-41
(P11103) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   171   2e-41
AK169326_1(AK169326|pid:none) Mus musculus 17 days pregnant adul...   171   2e-41
AK167699_1(AK167699|pid:none) Mus musculus 14 days pregnant adul...   171   2e-41
AK030889_1(AK030889|pid:none) Mus musculus adult male thymus cDN...   171   2e-41
AF126717_1(AF126717|pid:none) Mus musculus msPARP mRNA, complete...   171   3e-41
AK312339_1(AK312339|pid:none) Homo sapiens cDNA, FLJ92658, highl...   170   4e-41
(P35875) RecName: Full=Poly [ADP-ribose] polymerase;          Sh...   170   4e-41

 psg: 0.78  gvh: 0.60  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal

>> prediction for SFI255 is cyt

>SFI255F.Seq
AAAGTGATGAAGATGTAAAAACCACTACAACCACTACTACTTCAACATCAACAAAACCAC
TCACTGAAATTTTTCGATGGAATTGTATTTTGTTTTTCAGGTACATTTTCAGTAACACAA
ACTGAATTAACAAAAAAAGTTGAAGGTTGTGGCGCATCAACCTCAA----------

>SFI255Z.Seq
NNNNNNNNNNGAAACCAACCATTGTAAACATTTTCAAGATTGATCGTGATGGTGAAGCAG
ATCGTTATAAAACTAAAAAACATNTTGGTAATAGAAAACTCTTATGGCATGGTTCTCGTT
TAACAAATTATGCTTCCATCATTTCACAAGGTTTAANAATTGCTCCACCTGAAGCTCCAG
TTTCTGGTTATANATTTGGAAAAGGTGTTTATTTTGCTGATTGTATGTCACTTTCTGCAA
ATTATTGTAGAACAGTTGGATCTAATGATTTTTGTATGTTATTAGGTGATGTTGCATTGG
GTAAAACTGCAGATTTGGCTANAGATACTTATATGGAGAAACCACAACCAAATTNTCATT
CAACTTGGGCTTTAGGTACTGTTGAACCAGATCCAAAAGTATTTTTCCAAGATACTGAAG
GTTGTATCACTCCATACGGCCAAATGATTCCATCTCAACATAAAGGTGTTTCNTGTTATG
AACATCAATATGTCGTTTATGATGTTGCTCAAGTTCATTTGAAATATCTTTTGCAATTAA
GAAATTAATAAACTATATTTAATTGTATAATAATAATAATAATAATAAAATAAAAAAAAT
AAAAAAATAAAATAAAAANAATAATAAAA

>SFI255P.Seq
AAAGTGATGAAGATGTAAAAACCACTACAACCACTACTACTTCAACATCAACAAAACCAC
TCACTGAAATTTTTCGATGGAATTGTATTTTGTTTTTCAGGTACATTTTCAGTAACACAA
ACTGAATTAACAAAAAAAGTTGAAGGTTGTGGCGCATCAACCTCAA----------GAAA
CCAACCATTGTAAACATTTTCAAGATTGATCGTGATGGTGAAGCAGATCGTTATAAAACT
AAAAAACATNTTGGTAATAGAAAACTCTTATGGCATGGTTCTCGTTTAACAAATTATGCT
TCCATCATTTCACAAGGTTTAANAATTGCTCCACCTGAAGCTCCAGTTTCTGGTTATANA
TTTGGAAAAGGTGTTTATTTTGCTGATTGTATGTCACTTTCTGCAAATTATTGTAGAACA
GTTGGATCTAATGATTTTTGTATGTTATTAGGTGATGTTGCATTGGGTAAAACTGCAGAT
TTGGCTANAGATACTTATATGGAGAAACCACAACCAAATTNTCATTCAACTTGGGCTTTA
GGTACTGTTGAACCAGATCCAAAAGTATTTTTCCAAGATACTGAAGGTTGTATCACTCCA
TACGGCCAAATGATTCCATCTCAACATAAAGGTGTTTCNTGTTATGAACATCAATATGTC
GTTTATGATGTTGCTCAAGTTCATTTGAAATATCTTTTGCAATTAAGAAATTAATAAACT
ATATTTAATTGTATAATAATAATAATAATAATAAAATAAAAAAAATAAAAAAATAAAATA
AAAANAATAATAAAA