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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFI249
Library SF
(Link to library)
Clone ID SFI249
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID SFI249P
(Link to Original site)
Representative DNA sequence 
>SFI249 (SFI249Q) /CSM/SF/SFI2-C/SFI249Q.Seq.d/
ACTGTTGGCCTACTGGNATTTTTTCAAATTCATTTTTATTATACAAATATTAAATTCATA
TTTTATTTTTTTATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAAATTAAAA
AATCNCNTTTTTTGAAAAAAAAAAXXXXXXXXXXTAAAACCAAGATTCAAGATAAAGAAG
GTTTCCACCAGATCAACAACGTCTCATTTTCGCTGGTAACCAATTAGAAGATGGTCGTAC
TCTCTCTGATTACAATATCCAAAAAGAATCAACTCTCCACTTGGTCCTCAGATTGAGAGG
TGGTATGCAAATCTTTGTCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGG
CTCAGATAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGA
CCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTA
CAACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAAT
TTTTGTCAAAACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACAT
TGAGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCT
CATTTTCGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAA
GGAATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAAC
TCTCACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAA
AACAAAAATTCAAGACAAATAA
sequence update 2001.11.22
Translated Amino Acid sequence 
cwptgifsnsfllyky*ihilffyil*HFIKKKKKKKIKKSXFLKKK---

---KTKIQDKEGFHQINNVSFSLVTN*kmvvlslitiskknqlstwssd*evvckslskh
*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslittfkrnpls
i*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfspvnnwr
mvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkfktn
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxffqihfyytnikfifyffiycnis*kkkkkkklknxxf*kkk---

---*nqdsr*rrfppdqqrlifagnqledgrtlsdyniqkestlhlvlrlrggmqifvkt
ltgktitlevegsdnienvktkiqdkegippdqqrlifagkqledgrtlsdyniqkestl
hlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrlifagkqle
dgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvktkiqdk*

Frame B:
llaywxffkfifiiqilnsyfiflyivtfhkkkkkkkn*kixffekk---

---KTKIQDKEGFHQINNVSFSLVTN*kmvvlslitiskknqlstwssd*evvckslskh
*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslittfkrnpls
i*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfspvnnwr
mvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkfktn

Frame C:
cwptgifsnsfllyky*ihilffyil*HFIKKKKKKKIKKSXFLKKK---

---kprfkikkvstrsttshfrw*pirrwsysl*lqypkrinsplgpqierwyanlcqni
nw*dhhs*s*glr*y*kc*nkdsrqrryptrptkinfrw*tirrwsysl*lqhskgihsp
fspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnprqrrystrsttshfrr*tigg
wsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*ecknknsrqi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI249 (SFI249Q) /CSM/SF/SFI2-C/SFI249Q.Seq.d/                       1403   0.0
CFG260 (CFG260Q) /CSM/CF/CFG2-C/CFG260Q.Seq.d/                       1358   0.0
CFC580 (CFC580Q) /CSM/CF/CFC5-D/CFC580Q.Seq.d/                       1358   0.0
CFK168 (CFK168Q) /CSM/CF/CFK1-C/CFK168Q.Seq.d/                       1356   0.0
CFF493 (CFF493Q) /CSM/CF/CFF4-D/CFF493Q.Seq.d/                       1354   0.0
AFL649 (AFL649Q) /CSM/AF/AFL6-C/AFL649Q.Seq.d/                       1354   0.0
CHD734 (CHD734Q) /CSM/CH/CHD7-B/CHD734Q.Seq.d/                       1352   0.0
SFI459 (SFI459Q) /CSM/SF/SFI4-C/SFI459Q.Seq.d/                       1350   0.0
SFI250 (SFI250Q) /CSM/SF/SFI2-C/SFI250Q.Seq.d/                       1350   0.0
CFF858 (CFF858Q) /CSM/CF/CFF8-C/CFF858Q.Seq.d/                       1350   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.  1318   0.0    2
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.  1302   0.0    2
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.  1302   0.0    2
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   783   0.0    3
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   656   0.0    3
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   662   0.0    3
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   662   0.0    3
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   644   0.0    3
BJ528884|BJ528884.1 Oryzias latipes cDNA clone:MF01SSB034A18, 5' end single read.   192   e-108  4
BJ541175|BJ541175.1 Oryzias latipes cDNA clone:MF01SSB034A18, 3' end single read.   176   e-106  5
dna update 2003.12.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   437   e-121
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   437   e-121
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   437   e-121
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   434   e-120
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   422   e-117
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   420   e-116
S53719(S53719;S73107;S71069)polyubiquitin 6 - red alga (Gracilar...   420   e-116
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   420   e-116
S32020(S32020) polyubiquitin 6 - Geodia cydonium  &X70917_1(X709...   420   e-116
T16144(T16144) ubiquitin - Caenorhabditis elegans  &U23172_1(U23...   420   e-116
protein update 2009. 5.26
PSORT 

 psg: 0.59  gvh: 0.16  alm: 0.65  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.83  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: cytoplasmic

>> prediction for SFI249 is nuc
5' end seq. ID SFI249F
5' end seq. 
>SFI249F.Seq
ACTGTTGGCCTACTGGNATTTTTTCAAATTCATTTTTATTATACAAATATTAAATTCATA
TTTTATTTTTTTATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAAATTAAAA
AATCNCNTTTTTTGAAAAAAAAAA----------
Length of 5' end seq. 144
3' end seq. ID SFI249Z
3' end seq. 
>SFI249Z.Seq
NNNNNNNNNNTAAAACCAAGATTCAAGATAAAGAAGGTTTCCACCAGATCAACAACGTCT
CATTTTCGCTGGTAACCAATTAGAAGATGGTCGTACTCTCTCTGATTACAATATCCAAAA
AGAATCAACTCTCCACTTGGTCCTCAGATTGAGAGGTGGTATGCAAATCTTTGTCAAAAC
ATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGAAAATGTTAA
AACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTTTCGCTGG
TAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCT
CCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCACTGGTAA
GACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAAAATCCA
AGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACAATTGGA
GGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTTCT
CAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCATTACACT
CGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAAATAA
Length of 3' end seq. 718
Connected seq. ID SFI249P
Connected seq. 
>SFI249P.Seq
ACTGTTGGCCTACTGGNATTTTTTCAAATTCATTTTTATTATACAAATATTAAATTCATA
TTTTATTTTTTTATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAAATTAAAA
AATCNCNTTTTTTGAAAAAAAAAA----------TAAAACCAAGATTCAAGATAAAGAAG
GTTTCCACCAGATCAACAACGTCTCATTTTCGCTGGTAACCAATTAGAAGATGGTCGTAC
TCTCTCTGATTACAATATCCAAAAAGAATCAACTCTCCACTTGGTCCTCAGATTGAGAGG
TGGTATGCAAATCTTTGTCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGG
CTCAGATAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGA
CCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTA
CAACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAAT
TTTTGTCAAAACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACAT
TGAGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCT
CATTTTCGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAA
GGAATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAAC
TCTCACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAA
AACAAAAATTCAAGACAAATAA
Length of connected seq. 852
Full length Seq ID -
Full length Seq. -
Length of full length seq. -