>SFI247 (SFI247Q) /CSM/SF/SFI2-B/SFI247Q.Seq.d/
ACTGTTGGCCTACTGGNAAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAG
AATTGGAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTC
TGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTG
TAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTAT
TAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGG
AGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGC
AGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACA
AGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGG
CGGTGTAGAATCAATGAGTTTAAATCXXXXXXXXXXTCTGAAAATGTCGCAGAGCGTTTT
GGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCC
GCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGAT
GGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGAT
TTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGT
CAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTG
GGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATT
ATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACT
AAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGT
AAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGT
CATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAAGAGAATTACATCGT
CGNAAAGGTAAATAT

LLAYWXILHPITNKMSFNSRIGTISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLN---

---SENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKD
EGIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFV
AFAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNK
VNPNGSGIALGHPLGATGARQMATISRELHRRKGKY

Frame A:
tvgllxnitphn**nviqfknwnyfsslkhwtnfriksnfwis*irr*ycncctisysnl
*s*krsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*schiis
rftkgstmlysqqtmfirftsncqlcsssqsrfl*hwfsrrcrinefk---

---SENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKD
EGIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFV
AFAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNK
VNPNGSGIALGHPLGATGARQMATISRELHRRKGKY

Frame B:
LLAYWXILHPITNKMSFNSRIGTISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLN---

---lkmsqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssikt
kvsvrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*
llllsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrfwvwi*tk
liqmalvsl*vih*vllvpvkwlpfqenyivxkvn

Frame C:
cwptgkyytp*likchsiqelelflvt*aldqf*nqiqlldqlnqemil*llhhfvqqfv
klkeehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*q
vyqrkyhviqstdnvhqvykqlpivqqqskqvsmtlv*qav*nq*v*i---

---*kcrrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rr
ryp*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrs
fcccrcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs
*skwlwyrfrssircywcpsngyhfkritssxr*i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI247 (SFI247Q) /CSM/SF/SFI2-B/SFI247Q.Seq.d/                       2218   0.0
VFL606 (VFL606Q) /CSM/VF/VFL6-A/VFL606Q.Seq.d/                       1261   0.0
VFJ317 (VFJ317Q) /CSM/VF/VFJ3-A/VFJ317Q.Seq.d/                       1261   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       1261   0.0
VFD865 (VFD865Q) /CSM/VF/VFD8-C/VFD865Q.Seq.d/                       1261   0.0
SLG762 (SLG762Q) /CSM/SL/SLG7-C/SLG762Q.Seq.d/                       1261   0.0
SFK756 (SFK756Q) /CSM/SF/SFK7-C/SFK756Q.Seq.d/                       1261   0.0
SFH501 (SFH501Q) /CSM/SF/SFH5-A/SFH501Q.Seq.d/                       1261   0.0
SFG758 (SFG758Q) /CSM/SF/SFG7-C/SFG758Q.Seq.d/                       1261   0.0
SFG482 (SFG482Q) /CSM/SF/SFG4-D/SFG482Q.Seq.d/                       1261   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1261   0.0    7
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60   4e-11  4
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44   3e-10  4
CA917334|CA917334.1 EST641481 GPOD Medicago truncatula cDNA clone GPOD-35H18, mRNA sequence.    38   5e-10  5
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68   1e-09  3
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60   2e-09  3
CD007738|CD007738.1 VVB078G11_334604 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB078G11 5, mRNA sequence.    60   2e-08  2
CA515447|CA515447.1 KS09042A06 KS09 Capsicum annuum cDNA, mRNA sequence.    68   2e-08  2
CD716621|CD716621.1 VVB129E11_405541 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB129E11 5, mRNA sequence.    60   2e-08  2
CD008871|CD008871.1 VVB097G11_339261 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB097G11 5, mRNA sequence.    60   2e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   390   0.0
AF062589_2(AF062589|pid:none) Arabidopsis thaliana 3-keto-acyl-C...   239   e-102
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   235   e-102
AK230209_1(AK230209|pid:none) Arabidopsis thaliana mRNA for pero...   239   e-102
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   241   e-102
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   241   e-102
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   235   e-102
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   233   e-102
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   232   e-100
BT070433_1(BT070433|pid:none) Picea sitchensis clone WS02729_F15...   233   e-100

 psg: 0.90  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.36  mip: 0.05
 nuc: 0.02  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane

>> prediction for SFI247 is cyt

>SFI247F.Seq
ACTGTTGGCCTACTGGNAAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAG
AATTGGAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTC
TGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTG
TAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTAT
TAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGG
AGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGC
AGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACA
AGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGG
CGGTGTAGAATCAATGAGTTTAAATC----------

>SFI247Z.Seq
NNNNNNNNNNTCTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGA
ATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGA
TGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGA
CGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAA
AGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTG
TTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGT
AGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAAT
TCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAA
TGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAA
AGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCG
TCAAATGGCTACCATTTCAAGAGAATTACATCGTCGNAAAGGTAAATAT

>SFI247P.Seq
ACTGTTGGCCTACTGGNAAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAG
AATTGGAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTC
TGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTG
TAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTAT
TAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGG
AGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGC
AGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACA
AGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGG
CGGTGTAGAATCAATGAGTTTAAATC----------TCTGAAAATGTCGCAGAGCGTTTT
GGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCC
GCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGAT
GGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGAT
TTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGT
CAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTG
GGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATT
ATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACT
AAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGT
AAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGT
CATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAAGAGAATTACATCGT
CGNAAAGGTAAATAT