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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFI228
Library SF
(Link to library)
Clone ID SFI228
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16243-1
Original site URL
Representative seq. ID SFI228P
(Link to Original site)
Representative DNA sequence 
>SFI228 (SFI228Q) /CSM/SF/SFI2-B/SFI228Q.Seq.d/
ACTGTTGGCCTACTGGNTTTTTCATATTTATATATAATATTACACCCCATAACTAATAAA
ATGTCATTCAATTCAAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGNACCAATTTT
AGAATCAAATCCAACTTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACC
ATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAGCATTCATAGAGACAGCACCAGATGA
TTTATTAGCACCAGTTATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAAT
TGGAGATGTTGTTATGGGAGCAGTATTACCAAGATCATCACAAGGTGCAACCXXXXXXXX
XXGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACA
AGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCG
TAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAA
CCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCG
CTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTA
CCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTG
TCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAG
CTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAG
CATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTG
GTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAA
GAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATNCTNCTATGTGTATTGGTACGGC
NTAAGGTGCTGCA
sequence update 2001.11.22
Translated Amino Acid sequence 
llaywxfhiyi*yytp*likchsiqelklflvt*ALXPILESNPTSGSVKSGDDIVIVAP
FRTAICKAKRGAFIETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGAT---

---AERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGIR
EKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFAA
VGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNPN
GSGIALGHPLGATGARQMATISRELHRRKGKYGVXLLCVLVRXKVL
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxfsylyiilhpitnkmsfnsrietisrhlstgtnfriksnfwis*irr*ycncct
isysnl*s*krsihrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcn---

---AERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGIR
EKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFAA
VGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNPN
GSGIALGHPLGATGARQMATISRELHRRKGKYGVXLLCVLVRXKVL

Frame B:
llaywxfhiyi*yytp*likchsiqelklflvt*ALXPILESNPTSGSVKSGDDIVIVAP
FRTAICKAKRGAFIETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGAT---

---qsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssiktkvsv
rkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*llll
svsnhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrfwvwi*tkliqm
alvsl*vih*vllvpvkwlpfqenyivvkvnmv*xxyvywygxrcc

Frame C:
cwptgffifiynitphn**nviqfkn*nyfsslkhwxqf*nqiqlldqlnqemil*llhh
fvqqfvklkeehs*rqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvq---

---rafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rrryp*
enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrsfccc
rcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs*skw
lwyrfrssircywcpsngyhfkritss*r*iwcnxxmcigta*gaa
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI228 (SFI228Q) /CSM/SF/SFI2-B/SFI228Q.Seq.d/                       1959   0.0
SFG835 (SFG835Q) /CSM/SF/SFG8-B/SFG835Q.Seq.d/                       1289   0.0
VFJ317 (VFJ317Q) /CSM/VF/VFJ3-A/VFJ317Q.Seq.d/                       1287   0.0
SFK576 (SFK576Q) /CSM/SF/SFK5-D/SFK576Q.Seq.d/                       1287   0.0
SFK756 (SFK756Q) /CSM/SF/SFK7-C/SFK756Q.Seq.d/                       1281   0.0
AFG267 (AFG267Q) /CSM/AF/AFG2-C/AFG267Q.Seq.d/                       1281   0.0
VFL606 (VFL606Q) /CSM/VF/VFL6-A/VFL606Q.Seq.d/                       1277   0.0
SFI740 (SFI740Q) /CSM/SF/SFI7-B/SFI740Q.Seq.d/                       1277   0.0
SFB522 (SFB522Q) /CSM/SF/SFB5-A/SFB522Q.Seq.d/                       1277   0.0
CFF140 (CFF140Q) /CSM/CF/CFF1-B/CFF140Q.Seq.d/                       1277   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1259   0.0    10
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44   2e-10  4
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68   2e-08  2
CA515447|CA515447.1 KS09042A06 KS09 Capsicum annuum cDNA, mRNA sequence.    68   2e-08  2
BM066045|BM066045.1 KS07010C02 KS07 Capsicum annuum cDNA, mRNA sequence.    68   2e-08  2
CF609274|CF609274.1 INFIO01_000052 Grape Inflorescence pSPORT1 Library Vitis vinifera cDNA 5', mRNA sequence.    56   3e-08  3
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60   9e-08  2
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60   9e-08  2
AI563047|AI563047.1 EST00171 watermelon lambda zap library Citrullus lanatus cDNA clone WMLS336 5' similar to acetyl-CoA acyltransferase, mRNA sequence.    56   1e-07  2
AX619566|AX619566.1 Sequence 2529 from Patent WO02094868.          52   3e-07  4
dna update 2003.12.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   385   e-106
FJ502019_1(FJ502019|pid:none) Aristichthys nobilis clone A101 ac...   236   9e-61
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   234   5e-60
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   234   5e-60
AC126019_22(AC126019|pid:none) Medicago truncatula clone mth2-22...   233   6e-60
AF062589_2(AF062589|pid:none) Arabidopsis thaliana 3-keto-acyl-C...   232   1e-59
AK230209_1(AK230209|pid:none) Arabidopsis thaliana mRNA for pero...   232   2e-59
BC054299_1(BC054299|pid:none) Xenopus laevis acetyl-Coenzyme A a...   231   2e-59
M32801_1(M32801|pid:none) Rat 3-ketoacyl-CoA thiolase 2 mRNA, co...   230   7e-59
J02749_1(J02749|pid:none) Rat peroxisomal 3-ketoacyl-CoA thiolas...   230   7e-59
protein update 2009. 5.26
PSORT 

 psg: 0.75  gvh: 0.53  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.22  mip: 0.00
 nuc: 0.02  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SFI228 is end
5' end seq. ID SFI228F
5' end seq. 
>SFI228F.Seq
ACTGTTGGCCTACTGGNTTTTTCATATTTATATATAATATTACACCCCATAACTAATAAA
ATGTCATTCAATTCAAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGNACCAATTTT
AGAATCAAATCCAACTTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACC
ATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAGCATTCATAGAGACAGCACCAGATGA
TTTATTAGCACCAGTTATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAAT
TGGAGATGTTGTTATGGGAGCAGTATTACCAAGATCATCACAAGGTGCAACC--------
--
Length of 5' end seq. 352
3' end seq. ID SFI228Z
3' end seq. 
>SFI228Z.Seq
NNNNNNNNNNGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAA
TTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCC
AGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCG
TGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTAC
AACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAA
AAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGC
TGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTT
AAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGC
TTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAA
TGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTAC
CATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATNCTNCTATGTGTATT
GGTACGGCNTAAGGTGCTGCA
Length of 3' end seq. 681
Connected seq. ID SFI228P
Connected seq. 
>SFI228P.Seq
ACTGTTGGCCTACTGGNTTTTTCATATTTATATATAATATTACACCCCATAACTAATAAA
ATGTCATTCAATTCAAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGNACCAATTTT
AGAATCAAATCCAACTTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACC
ATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAGCATTCATAGAGACAGCACCAGATGA
TTTATTAGCACCAGTTATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAAT
TGGAGATGTTGTTATGGGAGCAGTATTACCAAGATCATCACAAGGTGCAACC--------
--GCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACA
AGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCG
TAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAA
CCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCG
CTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTA
CCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTG
TCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAG
CTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAG
CATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTG
GTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAA
GAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATNCTNCTATGTGTATTGGTACGGC
NTAAGGTGCTGCA
Length of connected seq. 1023
Full length Seq ID -
Full length Seq. -
Length of full length seq. -