>SFI222 (SFI222Q) /CSM/SF/SFI2-A/SFI222Q.Seq.d/
XXXXXXXXXXAGGTAGAATTAGAGCCATTCAATTAGAGTATGCTGAATCATTTAAATTCC
TCACACAAGCAATTAGAAAAGCACCACAAAACACTGCTGGTGGTTTCAGAAGAACTGTTT
ATAAACTTTTATCAATCGTTCAATTATTGATGGGTGAAATTCCAGAAAGAAATACTTTCT
CACAAAAACAATTAAAAATTGCACTCAAACCATACTTTCATTTAACTGAAGCCGTTAGAG
TTGGTGATTTAGGTTCTTTCAATCAAGCTTTGGAACAAAATTCAGACATTTTCAAATCAG
ATCAAACTTTTACCCTTGTTCAAAGATTACGTTCAAATGTTATCAAAGCTGGACTTAAAA
AATTAAATACTGCTTACTCTAGAATCTCTTTCAATGATATTTGTACAAAATTAAAATTTG
ATGGTACTACTCAAGATATCATGTTTATTATTGCAAAGACTATTAAAGATGGTGTTATTG
ATGCAACTATAAATTATGAAGGTGGTTACCTCCAAAGTAGAGAAAATATTGATGCTTATT
CAACTCAAGAACCATTACATGCTTTCTCCAATAGAATTGATATTTGTTTAAAGATTCACA
ATGATTCCCTTAAAGCTATGAGATTCCATCCAGATTTAAATAAAGCTGAAACTGAAAAGA
TTGCTGAAGTAGCCAAAACTATTAAAGAAGAAAGGAAAGACAAGCGAAGAAAGCTC

---GRIRAIQLEYAESFKFLTQAIRKAPQNTAGGFRRTVYKLLSIVQLLMGEIPERNTFS
QKQLKIALKPYFHLTEAVRVGDLGSFNQALEQNSDIFKSDQTFTLVQRLRSNVIKAGLKK
LNTAYSRISFNDICTKLKFDGTTQDIMFIIAKTIKDGVIDATINYEGGYLQSRENIDAYS
TQEPLHAFSNRIDICLKIHNDSLKAMRFHPDLNKAETEKIAEVAKTIKEERKDKRRKL

Frame A:
---GRIRAIQLEYAESFKFLTQAIRKAPQNTAGGFRRTVYKLLSIVQLLMGEIPERNTFS
QKQLKIALKPYFHLTEAVRVGDLGSFNQALEQNSDIFKSDQTFTLVQRLRSNVIKAGLKK
LNTAYSRISFNDICTKLKFDGTTQDIMFIIAKTIKDGVIDATINYEGGYLQSRENIDAYS
TQEPLHAFSNRIDICLKIHNDSLKAMRFHPDLNKAETEKIAEVAKTIKEERKDKRRKL


Frame B:
---velepfn*smlnhlnsshkqlekhhktllvvseelfinfyqsfny*wvkfqkeilsh
knn*klhsnhtfi*lkplelvi*vlsiklwnkiqtfsnqikllplfkdyvqmlskldlkn
*illtleslsmifvqn*nlmvllkisclllqrllkmvllmql*imkvvtskvekilmliq
lknhymlspielifv*rftmiplkl*dsiqi*iklklkrllk*pkllkkkgktsees


Frame C:
---*n*shsirvc*ii*iphtsn*ksttkhcwwfqkncl*tfinrsiidg*nsrkkyflt
ktiknctqtilsfn*sr*sw*frffqssfgtkfrhfqirsnfypcskitfkcyqswt*ki
kycll*nlfq*ylykiki*wyysryhvyyckdy*rwcy*cnykl*rwlppk*rky*clfn
srtitcflq*n*ylfkdsq*fp*syeipsrfk*s*n*kdc*ssqny*rrkerqakka

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI222 (SFI222Q) /CSM/SF/SFI2-A/SFI222Q.Seq.d/                       1400   0.0
VFJ116 (VFJ116Q) /CSM/VF/VFJ1-A/VFJ116Q.Seq.d/                       1394   0.0
AFL859 (AFL859Q) /CSM/AF/AFL8-C/AFL859Q.Seq.d/                       1394   0.0
VFD314 (VFD314Q) /CSM/VF/VFD3-A/VFD314Q.Seq.d/                       1390   0.0
AFE606 (AFE606Q) /CSM/AF/AFE6-A/AFE606Q.Seq.d/                       1384   0.0
AFC538 (AFC538Q) /CSM/AF/AFC5-B/AFC538Q.Seq.d/                       1384   0.0
SFD788 (SFD788Q) /CSM/SF/SFD7-D/SFD788Q.Seq.d/                       1376   0.0
AFG104 (AFG104Q) /CSM/AF/AFG1-A/AFG104Q.Seq.d/                       1291   0.0
SFK889 (SFK889Q) /CSM/SF/SFK8-D/SFK889Q.Seq.d/                       1263   0.0
AFK137 (AFK137Q) /CSM/AF/AFK1-B/AFK137Q.Seq.d/                       1253   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC093263|AC093263.3 Homo sapiens chromosome 5 clone RP11-259G23, complete sequence.    38   0.001  8
AC137994|AC137994.16 Medicago truncatula clone mth2-23f15, complete sequence.    32   0.004  9
AC139804|AC139804.1 Homo sapiens chromosome 5 clone RP11-1349B19, WORKING DRAFT SEQUENCE, 1 unordered piece.    38   0.005  8
BX255933|BX255933.7 Zebrafish DNA sequence from clone DKEY-38L7 in linkage group 6.    34   0.005  9
AE016747|AE016747.1 Staphylococcus epidermidis ATCC 12228, section 4 of 9 of the complete genome.    34   0.007  8
AZ528085|AZ528085.1 ENTCK89TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   0.013  3
BH164407|BH164407.1 ENTSU73TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   0.014  3
AL356096|AL356096.11 Human DNA sequence from clone RP11-366K1 on chromosome 13.    34   0.018  11
BX294180|BX294180.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    40   0.020  5
AR300198|AR300198.1 Sequence 1 from patent US 6537773.             32   0.020  14

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXD3) RecName: Full=26S proteasome non-ATPase regulatory subu...   445   e-124
AK145488_1(AK145488|pid:none) Mus musculus blastocyst blastocyst...   236   5e-61
D67025_1(D67025|pid:none) Homo sapiens mRNA for proteasome subun...   236   5e-61
AB169643_1(AB169643|pid:none) Macaca fascicularis brain cDNA, cl...   236   5e-61
AK298411_1(AK298411|pid:none) Homo sapiens cDNA FLJ55739 complet...   236   5e-61
AK054611_1(AK054611|pid:none) Homo sapiens cDNA FLJ30049 fis, cl...   236   5e-61
I49504(I49504) Tum-P91A antigen - mouse  &M25149_1(M25149|pid:no...   236   5e-61
BC085881_1(BC085881|pid:none) Rattus norvegicus proteasome (pros...   236   5e-61
AY891635_1(AY891635|pid:none) Synthetic construct Homo sapiens c...   236   5e-61
CR456772_1(CR456772|pid:none) Homo sapiens full open reading fra...   236   5e-61

 psg: 0.70  gvh: 0.50  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.02
 nuc: 0.04  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 24.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for SFI222 is nuc

>SFI222Z.Seq
NNNNNNNNNNAGGTAGAATTAGAGCCATTCAATTAGAGTATGCTGAATCATTTAAATTCC
TCACACAAGCAATTAGAAAAGCACCACAAAACACTGCTGGTGGTTTCAGAAGAACTGTTT
ATAAACTTTTATCAATCGTTCAATTATTGATGGGTGAAATTCCAGAAAGAAATACTTTCT
CACAAAAACAATTAAAAATTGCACTCAAACCATACTTTCATTTAACTGAAGCCGTTAGAG
TTGGTGATTTAGGTTCTTTCAATCAAGCTTTGGAACAAAATTCAGACATTTTCAAATCAG
ATCAAACTTTTACCCTTGTTCAAAGATTACGTTCAAATGTTATCAAAGCTGGACTTAAAA
AATTAAATACTGCTTACTCTAGAATCTCTTTCAATGATATTTGTACAAAATTAAAATTTG
ATGGTACTACTCAAGATATCATGTTTATTATTGCAAAGACTATTAAAGATGGTGTTATTG
ATGCAACTATAAATTATGAAGGTGGTTACCTCCAAAGTAGAGAAAATATTGATGCTTATT
CAACTCAAGAACCATTACATGCTTTCTCCAATAGAATTGATATTTGTTTAAAGATTCACA
ATGATTCCCTTAAAGCTATGAGATTCCATCCAGATTTAAATAAAGCTGAAACTGAAAAGA
TTGCTGAAGTAGCCAAAACTATTAAAGAAGAAAGGAAAGACAAGCGAAGAAAGCTC