>SFI219 (SFI219Q) /CSM/SF/SFI2-A/SFI219Q.Seq.d/
ACTGTTGGCCTACTGGNAATTTTTTTATTTTTTAATTTATTAATAACCAAAATTTCAAAT
ATTTCAACTATTTTGTATTTGTTTTTAATACACATAAAATGGATTCATTTGTATTAGATG
CACAAAACCCAAACCCAAAAAATTTAAAAAATTTATTAAAATATGAAGAACAACTCGAAA
GACAACATGTCTCATTAGATAATATTTTAGGCGCCCTTGATGTCAGAAATCATTCATTAG
GTCAACTTTTAGTATTAAAAGCCAAAGGCAGTGATCAAGGTAAAAATCGTTCTGTTTTTA
TTGATCAATGTAATAATTTCTTTAGAAATTGTAATGTTGAACAAGTTAGATTAGCACCAG
CTCAATTATCACAACTTAGTAAATTTTATACAGAAGCATTATATGAATTAAAGCAACCAA
TTAGAGGTGTTGCAGTACTCAAAGATGCACTTAATATTTTATCCGATAATAAACCAACAA
CCACTCTTACACCAATTCATACAGATTTTTTACAACTTTGTATCCTTTCAAAATGTTATC
ATCAAGCACTCCCATTAATTGAAAGTAACATCACTCACATTAATCXXXXXXXXXXCGCCA
TCAAAGATATTCTTTGTTACTTTTACTATTCCTGGTATCATTTTCACTGCTTTTAAGAAA
TATAAAAAAGCAATTGAAGCTTTTAAATTTGTTATTACTGCACCAGCATCAGCATTAAGT
GCAATTACAGTTGAAGCTTATAAAAAATATTTAATTGTATATTTAATTCAATTTGGTTCA
GTTCAACATTTCCCAAAATGTACACCAGCAGTTGTTCAAAGAAATATTAAATCTCATTGT
AAACCATACACAGAATTTGTTCAATCCTTTTCAAATGGTAATATAAATGATATTATGAAT
AAAGCTTCATCTGGTGCTGAATTCTTCCAAAAGGATAGCAACTGGGGTTTAGTTAAACTT
TCAATTAAATCAACCTATCGTAGAAATATCAAAAAACTCACTCAAACTTTTATGACTCTT
TCAATTAATGATATTGCTGAAAAGGTTTCTCTTCCAAAAGCAAAAGCTGAACAATTCGTT
TTAAAGATGATTGAAGAAGGTGAAATTTTTGCAACTATTAATCAAAAAGATGGTATGGTT
ACTTTTGATGAATGTTTTGAAGATTTTAGTGGTCCAACTATCTTAAATGATTTGGATCGT
AATATCAACAATATTGTTGGTTTAGAATCAAAGATTAAAGAGATGGATGAAAAGATTTCA
TTAAGTAATAATTANNAAAAAAAA

cwptgnffif*FINNQNFKYFNYFVFVFNTHKMDSFVLDAQNPNPKNLKNLLKYEEQLER
QHVSLDNILGALDVRNHSLGQLLVLKAKGSDQGKNRSVFIDQCNNFFRNCNVEQVRLAPA
QLSQLSKFYTEALYELKQPIRGVAVLKDALNILSDNKPTTTLTPIHTDFLQLCILSKCYH
QALPLIESNITHIN---

---PSKIFFVTFTIPGIIFTAFKKYKKAIEAFKFVITAPASALSAITVEAYKKYLIVYLI
QFGSVQHFPKCTPAVVQRNIKSHCKPYTEFVQSFSNGNINDIMNKASSGAEFFQKDSNWG
LVKLSIKSTYRRNIKKLTQTFMTLSINDIAEKVSLPKAKAEQFVLKMIEEGEIFATINQK
DGMVTFDECFEDFSGPTILNDLDRNINNIVGLESKIKEMDEKISLSNNXXKK

Frame A:
tvgllxiflffnllitkisnistilylflihikwihly*mhktqtqki*kiy*nmknnsk
dnmsh*iif*aplmseiih*vnf*y*kpkavikvkivlfllinviisleivmlnkld*hq
lnyhnlvnfiqkhymn*snqlevlqyskmhlifypiinqqpllhqfiqifynfvsfqnvi
ikhsh*lkvtsltli---

---rhqryslllllflvsfslllrnikkqlkllnllllhqhqh*vqlqlklikni*lyi*
fnlvqfnisqnvhqqlfkeilnlivnhtqnlfnpfqmvi*mil*iklhlvlnsskriatg
v*lnfqlnqpiveisknslkll*lfqlmillkrflfqkqklnnsf*r*lkkvkflqllik
kmvwlllmnvlkilvvqls*miwivistillv*nqrlkrwmkrfh*viixkk

Frame B:
llaywxffyfliy**pkfqifqlfcicf*yt*ngficirctkpkpkkfkkfiki*rttrk
ttclir*yfrrp*cqksfirstfsiksqrq*sr*ksfcfy*sm**fl*kl*c*ts*ists
siitt**ilyrsii*ikatn*rccstqrct*yfir**tnnhsytnsyrffttlypfkmls
sstpin*k*hhsh*---

---aikdilcyfyyswyhfhcf*ei*ksn*sf*icyyctsisikcnys*sl*kifncifn
siwfsstfpkmytsscskky*isl*tihricsilfkw*yk*yye*sfiwc*ilpkg*qlg
fs*tfn*inls*kyqkthsnfydsfn**yc*kgfssksks*tirfkdd*rr*nfcny*sk
rwygyf**mf*rf*wsnylk*fgs*yqqycwfrikd*rdg*kdfik**lxkk

Frame C:
cwptgnffif*FINNQNFKYFNYFVFVFNTHKMDSFVLDAQNPNPKNLKNLLKYEEQLER
QHVSLDNILGALDVRNHSLGQLLVLKAKGSDQGKNRSVFIDQCNNFFRNCNVEQVRLAPA
QLSQLSKFYTEALYELKQPIRGVAVLKDALNILSDNKPTTTLTPIHTDFLQLCILSKCYH
QALPLIESNITHIN---

---PSKIFFVTFTIPGIIFTAFKKYKKAIEAFKFVITAPASALSAITVEAYKKYLIVYLI
QFGSVQHFPKCTPAVVQRNIKSHCKPYTEFVQSFSNGNINDIMNKASSGAEFFQKDSNWG
LVKLSIKSTYRRNIKKLTQTFMTLSINDIAEKVSLPKAKAEQFVLKMIEEGEIFATINQK
DGMVTFDECFEDFSGPTILNDLDRNINNIVGLESKIKEMDEKISLSNNXXKK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI219 (SFI219Q) /CSM/SF/SFI2-A/SFI219Q.Seq.d/                       2147   0.0
VFI631 (VFI631Q) /CSM/VF/VFI6-B/VFI631Q.Seq.d/                       1340   0.0
VFH852 (VFH852Q) /CSM/VF/VFH8-C/VFH852Q.Seq.d/                       1338   0.0
VFI138 (VFI138Q) /CSM/VF/VFI1-B/VFI138Q.Seq.d/                       1330   0.0
SFG708 (SFG708Q) /CSM/SF/SFG7-A/SFG708Q.Seq.d/                       1330   0.0
SFE815 (SFE815Q) /CSM/SF/SFE8-A/SFE815Q.Seq.d/                       1330   0.0
CFA641 (CFA641Q) /CSM/CF/CFA6-B/CFA641Q.Seq.d/                       1330   0.0
AFO448 (AFO448Q) /CSM/AF/AFO4-B/AFO448Q.Seq.d/                       1330   0.0
AFH628 (AFH628Q) /CSM/AF/AFH6-B/AFH628Q.Seq.d/                       1330   0.0
VFH780 (VFH780Q) /CSM/VF/VFH7-D/VFH780Q.Seq.d/                       1324   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE014841|AE014841.1 Plasmodium falciparum 3D7 chromosome 11 section 6 of 8 of the complete sequence.    38   2e-13  18
AC004709|AC004709.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 3 unordered pieces.    38   2e-08  17
AE014847|AE014847.1 Plasmodium falciparum 3D7 chromosome 12, section 4 of 9 of the complete sequence.    38   3e-08  18
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    32   2e-06  25
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    32   2e-06  18
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           36   2e-06  24
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            38   2e-06  28
BX248585|BX248585.1 Blochmannia floridanus complete genome; segment 2/3.    36   3e-06  18
AL929356|AL929356.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 2/5.    36   5e-06  19
AP006628|AP006628.1 Onion yellows phytoplasma DNA, complete genome.    36   2e-05  27

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT065643_1(BT065643|pid:none) Zea mays full-length cDNA clone ZM...   173   1e-57
EU963506_1(EU963506|pid:none) Zea mays clone 263419 COP9 signalo...   170   6e-57
EU962982_1(EU962982|pid:none) Zea mays clone 247057 COP9 signalo...   170   8e-57
AC138464_5(AC138464|pid:none) Medicago truncatula clone mth2-29i...   154   1e-52
BT052055_1(BT052055|pid:none) Medicago truncatula clone MTYF9_FA...   149   5e-51
AF129081_1(AF129081|pid:none) Drosophila melanogaster COP9 signa...   154   4e-49
BT044093_1(BT044093|pid:none) Drosophila melanogaster FI02069 fu...   154   7e-49
AY071575_1(AY071575|pid:none) Drosophila melanogaster RE63441 fu...   154   9e-49
AF419585_1(AF419585|pid:none) Arabidopsis thaliana AT5g14250/F18...   152   1e-47
AL163817_4(AL163817|pid:none) Arabidopsis thaliana DNA chromosom...   144   4e-45

 psg: 0.90  gvh: 0.30  alm: 0.33  top: 0.63  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for SFI219 is nuc

>SFI219F.Seq
ACTGTTGGCCTACTGGNAATTTTTTTATTTTTTAATTTATTAATAACCAAAATTTCAAAT
ATTTCAACTATTTTGTATTTGTTTTTAATACACATAAAATGGATTCATTTGTATTAGATG
CACAAAACCCAAACCCAAAAAATTTAAAAAATTTATTAAAATATGAAGAACAACTCGAAA
GACAACATGTCTCATTAGATAATATTTTAGGCGCCCTTGATGTCAGAAATCATTCATTAG
GTCAACTTTTAGTATTAAAAGCCAAAGGCAGTGATCAAGGTAAAAATCGTTCTGTTTTTA
TTGATCAATGTAATAATTTCTTTAGAAATTGTAATGTTGAACAAGTTAGATTAGCACCAG
CTCAATTATCACAACTTAGTAAATTTTATACAGAAGCATTATATGAATTAAAGCAACCAA
TTAGAGGTGTTGCAGTACTCAAAGATGCACTTAATATTTTATCCGATAATAAACCAACAA
CCACTCTTACACCAATTCATACAGATTTTTTACAACTTTGTATCCTTTCAAAATGTTATC
ATCAAGCACTCCCATTAATTGAAAGTAACATCACTCACATTAATC----------

>SFI219Z.Seq
NNNNNNNNNNCGCCATCAAAGATATTCTTTGTTACTTTTACTATTCCTGGTATCATTTTC
ACTGCTTTTAAGAAATATAAAAAAGCAATTGAAGCTTTTAAATTTGTTATTACTGCACCA
GCATCAGCATTAAGTGCAATTACAGTTGAAGCTTATAAAAAATATTTAATTGTATATTTA
ATTCAATTTGGTTCAGTTCAACATTTCCCAAAATGTACACCAGCAGTTGTTCAAAGAAAT
ATTAAATCTCATTGTAAACCATACACAGAATTTGTTCAATCCTTTTCAAATGGTAATATA
AATGATATTATGAATAAAGCTTCATCTGGTGCTGAATTCTTCCAAAAGGATAGCAACTGG
GGTTTAGTTAAACTTTCAATTAAATCAACCTATCGTAGAAATATCAAAAAACTCACTCAA
ACTTTTATGACTCTTTCAATTAATGATATTGCTGAAAAGGTTTCTCTTCCAAAAGCAAAA
GCTGAACAATTCGTTTTAAAGATGATTGAAGAAGGTGAAATTTTTGCAACTATTAATCAA
AAAGATGGTATGGTTACTTTTGATGAATGTTTTGAAGATTTTAGTGGTCCAACTATCTTA
AATGATTTGGATCGTAATATCAACAATATTGTTGGTTTAGAATCAAAGATTAAAGAGATG
GATGAAAAGATTTCATTAAGTAATAATTANNAAAAAAAA

>SFI219P.Seq
ACTGTTGGCCTACTGGNAATTTTTTTATTTTTTAATTTATTAATAACCAAAATTTCAAAT
ATTTCAACTATTTTGTATTTGTTTTTAATACACATAAAATGGATTCATTTGTATTAGATG
CACAAAACCCAAACCCAAAAAATTTAAAAAATTTATTAAAATATGAAGAACAACTCGAAA
GACAACATGTCTCATTAGATAATATTTTAGGCGCCCTTGATGTCAGAAATCATTCATTAG
GTCAACTTTTAGTATTAAAAGCCAAAGGCAGTGATCAAGGTAAAAATCGTTCTGTTTTTA
TTGATCAATGTAATAATTTCTTTAGAAATTGTAATGTTGAACAAGTTAGATTAGCACCAG
CTCAATTATCACAACTTAGTAAATTTTATACAGAAGCATTATATGAATTAAAGCAACCAA
TTAGAGGTGTTGCAGTACTCAAAGATGCACTTAATATTTTATCCGATAATAAACCAACAA
CCACTCTTACACCAATTCATACAGATTTTTTACAACTTTGTATCCTTTCAAAATGTTATC
ATCAAGCACTCCCATTAATTGAAAGTAACATCACTCACATTAATC----------CGCCA
TCAAAGATATTCTTTGTTACTTTTACTATTCCTGGTATCATTTTCACTGCTTTTAAGAAA
TATAAAAAAGCAATTGAAGCTTTTAAATTTGTTATTACTGCACCAGCATCAGCATTAAGT
GCAATTACAGTTGAAGCTTATAAAAAATATTTAATTGTATATTTAATTCAATTTGGTTCA
GTTCAACATTTCCCAAAATGTACACCAGCAGTTGTTCAAAGAAATATTAAATCTCATTGT
AAACCATACACAGAATTTGTTCAATCCTTTTCAAATGGTAATATAAATGATATTATGAAT
AAAGCTTCATCTGGTGCTGAATTCTTCCAAAAGGATAGCAACTGGGGTTTAGTTAAACTT
TCAATTAAATCAACCTATCGTAGAAATATCAAAAAACTCACTCAAACTTTTATGACTCTT
TCAATTAATGATATTGCTGAAAAGGTTTCTCTTCCAAAAGCAAAAGCTGAACAATTCGTT
TTAAAGATGATTGAAGAAGGTGAAATTTTTGCAACTATTAATCAAAAAGATGGTATGGTT
ACTTTTGATGAATGTTTTGAAGATTTTAGTGGTCCAACTATCTTAAATGATTTGGATCGT
AATATCAACAATATTGTTGGTTTAGAATCAAAGATTAAAGAGATGGATGAAAAGATTTCA
TTAAGTAATAATTANNAAAAAAAA