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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFI213
Library SF
(Link to library)
Clone ID SFI213
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFI213P
(Link to Original site)
Representative DNA sequence 
>SFI213 (SFI213Q) /CSM/SF/SFI2-A/SFI213Q.Seq.d/
AGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAATTTTTTTGT
ATATAAATATTTTGTAAATAAATAAATANATAATAATTTTTTTTTTAATTCACACNCATA
CATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGNTTTAGGTA
CAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATC
XXXXXXXXXXTCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCA
AAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTG
TACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTG
AAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTT
CAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAAC
AACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAA
ATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTG
AATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATG
AATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCT
ATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCAC
CAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGA
sequence update 2001.11.22
Translated Amino Acid sequence 
fnliynnnnsini*rliflyinil*inkxiiifflihthtyky*iiky*KMSSIGIXLGT
TYSCVGVWQNDRVEIIAND---

---QPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAE
DKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKN
SIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLY
QEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfse
Translated Amino Acid sequence (All Frames) 
Frame A:
ssi*fiiiiiv*iykd*ffci*ifck*inx**fff*ftxihinik*lnikkchqlvlx*v
qhihvlvfgkmivlkllqmi---

---strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkcis*
r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc*k
fn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*tl
srrryatrwwyarwyvk*ftkefkqqsrrirlteffrg

Frame B:
vqfnl*****ykyikinffvykyfvnk*ixnnfffnshxyi*ilnn*ilknvinwyxfry
nifmcwclak*sc*nyck*---

---QPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAE
DKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKN
SIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLY
QEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfse

Frame C:
fnliynnnnsini*rliflyinil*inkxiiifflihthtyky*iiky*KMSSIGIXLGT
TYSCVGVWQNDRVEIIAND---

---nqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*myqlk
inqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsllki
qlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*vnsi
kkevchkvvvcqvvcqmihqrvqttk*tn*idgifqr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1279   0.0
VHQ240 (VHQ240Q) /CSM/VH/VHQ2-B/VHQ240Q.Seq.d/                       1279   0.0
VHP873 (VHP873Q) /CSM/VH/VHP8-D/VHP873Q.Seq.d/                       1279   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1279   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1279   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1279   0.0
VHP420 (VHP420Q) /CSM/VH/VHP4-A/VHP420Q.Seq.d/                       1279   0.0
VHP266 (VHP266Q) /CSM/VH/VHP2-C/VHP266Q.Seq.d/                       1279   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1279   0.0
VHP232 (VHP232Q) /CSM/VH/VHP2-B/VHP232Q.Seq.d/                       1279   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1279   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1271   0.0    1
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          129   1e-50  5
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 129   2e-50  5
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   129   3e-50  5
M84652|M84652.1 Entamoeba heat shock protein (Hsp70) mRNA, complete cds.   125   2e-49  5
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   111   1e-48  5
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   111   6e-46  11
AF278536|AF278536.1 Parastrongyloides trichosuri heat shock 70 protein gene, complete cds.   143   6e-45  5
AJ310880|AJ310880.1 Cryptosporidium baileyi partial hsp70 gene for heat shock protein  70.    96   3e-44  4
dna update 2003.12.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   347   2e-94
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   347   2e-94
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   253   8e-66
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   252   1e-65
EF506932_1(EF506932|pid:none) Fasciola hepatica heat shock prote...   251   2e-65
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   251   2e-65
(P22953) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   251   3e-65
S46302(S46302;S41402;JA0169;S36623) dnaK-type molecular chaperon...   251   3e-65
AY035123_1(AY035123|pid:none) Arabidopsis thaliana putative dnaK...   251   3e-65
EF506931_1(EF506931|pid:none) Fasciola gigantica heat shock prot...   250   4e-65
protein update 2009. 5.25
PSORT 

 psg: 0.91  gvh: 0.43  alm: 0.43  top: 0.27  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SFI213 is cyt
5' end seq. ID SFI213F
5' end seq. 
>SFI213F.Seq
AGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAATTTTTTTGT
ATATAAATATTTTGTAAATAAATAAATANATAATAATTTTTTTTTTAATTCACACNCATA
CATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGNTTTAGGTA
CAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATC
----------
Length of 5' end seq. 240
3' end seq. ID SFI213Z
3' end seq. 
>SFI213Z.Seq
NNNNNNNNNNTCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCA
AAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTG
TACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTG
AAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTT
CAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAAC
AACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAA
ATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTG
AATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATG
AATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCT
ATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCAC
CAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGA
Length of 3' end seq. 655
Connected seq. ID SFI213P
Connected seq. 
>SFI213P.Seq
AGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAATTTTTTTGT
ATATAAATATTTTGTAAATAAATAAATANATAATAATTTTTTTTTTAATTCACACNCATA
CATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGNTTTAGGTA
CAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATC
----------TCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCA
AAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTG
TACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTG
AAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTT
CAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAAC
AACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAA
ATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTG
AATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATG
AATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCT
ATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCAC
CAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGA
Length of connected seq. 885
Full length Seq ID -
Full length Seq. -
Length of full length seq. -